BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q969H6 (163 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AHR-E nrPDB 336 2e-93 6AH3-E nrPDB 46 7e-06 2CZV-C nrPDB 32 0.094 5O9H-A nrPDB 31 0.25 6C1Q-B nrPDB 30 0.44 2CZH-A nrPDB 27 4.8 3CVR-A nrPDB 26 7.6 >6AHR-E nrPDB Length = 163 Score = 336 bits (862), Expect = 2e-93, Method: Compositional matrix adjust. Identities = 163/163 (100%), Positives = 163/163 (100%) Query: 1 MVRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNA 60 MVRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNA Sbjct: 1 MVRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNA 60 Query: 61 YTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRR 120 YTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRR Sbjct: 61 YTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRR 120 Query: 121 QLLILLQNCTDEGEREAIQKSVTRSCLLEEEEESGEEAAEAME 163 QLLILLQNCTDEGEREAIQKSVTRSCLLEEEEESGEEAAEAME Sbjct: 121 QLLILLQNCTDEGEREAIQKSVTRSCLLEEEEESGEEAAEAME 163 >6AH3-E nrPDB Length = 173 Score = 45.8 bits (107), Expect = 7e-06, Method: Compositional matrix adjust. Identities = 37/144 (25%), Positives = 66/144 (45%), Gaps = 22/144 (15%) Query: 1 MVRFKHRYLLCELV--SDDPRC--------------RLSLDDRVLSSL---VRDTIARVH 41 MVR K RY+L E++ D R S D + S+ +R +++ Sbjct: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 Query: 42 GTFGAAACSIGFAVRYLNAYTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTL 101 G +G+A C+ ++Y + T ++RC +E LV AL ++ + G N + Sbjct: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKI---GDVDGLIVNPV 117 Query: 102 HVGGTIRTCQKFLIQYNRRQLLIL 125 V GTI+ ++F ++ N + L I+ Sbjct: 118 KVSGTIKKIEQFAMRRNSKILNII 141 >2CZV-C nrPDB Length = 120 Score = 32.3 bits (72), Expect = 0.094, Method: Compositional matrix adjust. Identities = 37/118 (31%), Positives = 53/118 (44%), Gaps = 12/118 (10%) Query: 2 VRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNAY 61 +R K+RY+ E++SD + D V L+ + V G G A N Sbjct: 12 LRDKNRYIAFEIISDGDFTK----DEV-KELIWKSSLEVLGETGTAIVKPWLIKFDPNTK 66 Query: 62 TGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTI-RTCQKFLIQYN 118 TGIV R +E+ + + AL ++ E G R TL V GTI R +KFL +Y Sbjct: 67 TGIV--RSDREYVEYLRFALMLVS--EFNGKR--LIIRTLGVSGTIKRLKRKFLAKYG 118 >5O9H-A nrPDB Length = 317 Score = 30.8 bits (68), Expect = 0.25, Method: Compositional matrix adjust. Identities = 16/39 (41%), Positives = 21/39 (53%) Query: 29 LSSLVRDTIARVHGTFGAAACSIGFAVRYLNAYTGIVLL 67 L +L + H FG AACSI ++ LN Y I+LL Sbjct: 60 LPALFTSIVQHHHWPFGGAACSILPSLILLNMYASILLL 98 >6C1Q-B nrPDB Length = 422 Score = 30.0 bits (66), Expect = 0.44, Method: Compositional matrix adjust. Identities = 14/27 (51%), Positives = 17/27 (62%) Query: 41 HGTFGAAACSIGFAVRYLNAYTGIVLL 67 H FG AACSI ++ LN Y I+LL Sbjct: 187 HWPFGGAACSILPSLILLNMYASILLL 213 >2CZH-A nrPDB Length = 299 Score = 26.6 bits (57), Expect = 4.8, Method: Compositional matrix adjust. Identities = 19/55 (34%), Positives = 26/55 (47%), Gaps = 2/55 (3%) Query: 3 RF-KHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVR 56 RF HR++ E + +C L+ + + T VH F A SIGFAVR Sbjct: 83 RFPSHRFIAEEAAASGAKCVLTHSPTWIIDPIDGTCNFVH-RFPTVAVSIGFAVR 136 >3CVR-A nrPDB Length = 571 Score = 26.2 bits (56), Expect = 7.6, Method: Compositional matrix adjust. Identities = 10/27 (37%), Positives = 20/27 (74%) Query: 13 LVSDDPRCRLSLDDRVLSSLVRDTIAR 39 ++S DP C + L+D LSS +R+++++ Sbjct: 226 ILSLDPTCTIILEDNPLSSRIRESLSQ 252 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AHR-E nrPDB 274 1e-74 6AH3-E nrPDB 162 8e-41 Sequences not found previously or not previously below threshold: 2CZV-C nrPDB 47 5e-06 3WZ0-C nrPDB 40 4e-04 4P1T-A nrPDB 28 2.6 5OC1-A nrPDB 27 3.0 5O9H-A nrPDB 27 4.4 5NJ6-A nrPDB 26 8.0 5NDD-A nrPDB 26 8.0 >6AHR-E nrPDB Length = 163 Score = 274 bits (701), Expect = 1e-74, Method: Composition-based stats. Identities = 163/163 (100%), Positives = 163/163 (100%) Query: 1 MVRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNA 60 MVRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNA Sbjct: 1 MVRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNA 60 Query: 61 YTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRR 120 YTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRR Sbjct: 61 YTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQKFLIQYNRR 120 Query: 121 QLLILLQNCTDEGEREAIQKSVTRSCLLEEEEESGEEAAEAME 163 QLLILLQNCTDEGEREAIQKSVTRSCLLEEEEESGEEAAEAME Sbjct: 121 QLLILLQNCTDEGEREAIQKSVTRSCLLEEEEESGEEAAEAME 163 >6AH3-E nrPDB Length = 173 Score = 162 bits (409), Expect = 8e-41, Method: Composition-based stats. Identities = 37/144 (25%), Positives = 66/144 (45%), Gaps = 22/144 (15%) Query: 1 MVRFKHRYLLCELV--SDDPRC--------------RLSLDDRVLSSL---VRDTIARVH 41 MVR K RY+L E++ D R S D + S+ +R +++ Sbjct: 1 MVRLKSRYILFEIIFPPTDTNVEESVSKADILLSHHRASPADVSIKSILQEIRRSLSLNL 60 Query: 42 GTFGAAACSIGFAVRYLNAYTGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTL 101 G +G+A C+ ++Y + T ++RC +E LV AL ++ + G N + Sbjct: 61 GDYGSAKCNSLLQLKYFSNKTSTGIIRCHREDCDLVIMALMLMSKI---GDVDGLIVNPV 117 Query: 102 HVGGTIRTCQKFLIQYNRRQLLIL 125 V GTI+ ++F ++ N + L I+ Sbjct: 118 KVSGTIKKIEQFAMRRNSKILNII 141 >2CZV-C nrPDB Length = 120 Score = 46.5 bits (109), Expect = 5e-06, Method: Composition-based stats. Identities = 28/120 (23%), Positives = 50/120 (41%), Gaps = 12/120 (10%) Query: 2 VRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNAY 61 +R K+RY+ E++SD + + L+ + V G G A + Sbjct: 12 LRDKNRYIAFEIISDGDFTKDE-----VKELIWKSSLEVLGETGTAIVKPWLI--KFDPN 64 Query: 62 TGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQ-KFLIQYNRR 120 T ++R +E+ + + AL ++ K TL V GTI+ + KFL +Y + Sbjct: 65 TKTGIVRSDREYVEYLRFALMLVSEFNGK----RLIIRTLGVSGTIKRLKRKFLAKYGWK 120 >3WZ0-C nrPDB Length = 120 Score = 40.0 bits (92), Expect = 4e-04, Method: Composition-based stats. Identities = 26/120 (21%), Positives = 54/120 (45%), Gaps = 12/120 (10%) Query: 2 VRFKHRYLLCELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNAY 61 +R K+RY+ +++ + P + D + ++ +++ G G+A F + Sbjct: 12 LRDKNRYIAFQVIGERPFKK----DEIKKAVWEASLS-ALGYLGSARAKPWFI--KFDEK 64 Query: 62 TGIVLLRCRKEFYQLVWSALPFITYLENKGHRYPCFFNTLHVGGTIRTCQ-KFLIQYNRR 120 + ++R ++ + + AL +T + F TL V GTI+ + KFL +Y R Sbjct: 65 SQTGIVRVDRKHVEELRFALTMLTEINGS----KVIFRTLGVSGTIKRLKRKFLAEYGWR 120 >4P1T-A nrPDB Length = 746 Score = 27.7 bits (60), Expect = 2.6, Method: Composition-based stats. Identities = 16/84 (19%), Positives = 30/84 (35%), Gaps = 2/84 (2%) Query: 18 PRCRLSLDDRVLSSLVRDTIARVHGTFGA-AACSIGFAVRYLNAYTGIVLLRCRKEFYQL 76 P C + D + + + +G + + +C +Y NA L C ++ + Sbjct: 334 PECISANFDFIFNDNIEYKTYYPYGDYSSICSCEQVKYYKYNNAEKKNNKLLCYEKDNDM 393 Query: 77 VWSALPFITYLENKGHRYPCFFNT 100 WS +I LEN + Sbjct: 394 TWSK-KYIKKLENGRSLEGVYVPP 416 >5OC1-A nrPDB Length = 565 Score = 27.3 bits (59), Expect = 3.0, Method: Composition-based stats. Identities = 12/40 (30%), Positives = 17/40 (42%) Query: 11 CELVSDDPRCRLSLDDRVLSSLVRDTIARVHGTFGAAACS 50 + DPR R DD + S +RD + G A+ S Sbjct: 470 FVIRPFDPRLRDPTDDAAIESYIRDNANTIFHPVGTASMS 509 >5O9H-A nrPDB Length = 317 Score = 26.9 bits (58), Expect = 4.4, Method: Composition-based stats. Identities = 17/44 (38%), Positives = 22/44 (50%) Query: 24 LDDRVLSSLVRDTIARVHGTFGAAACSIGFAVRYLNAYTGIVLL 67 L L +L + H FG AACSI ++ LN Y I+LL Sbjct: 55 LACLALPALFTSIVQHHHWPFGGAACSILPSLILLNMYASILLL 98 >5NJ6-A nrPDB Length = 437 Score = 26.1 bits (56), Expect = 8.0, Method: Composition-based stats. Identities = 9/26 (34%), Positives = 13/26 (50%) Query: 44 FGAAACSIGFAVRYLNAYTGIVLLRC 69 +G A C++ Y N Y I+ L C Sbjct: 90 YGEALCNVLIGFFYANMYCSILFLTC 115 >5NDD-A nrPDB Length = 619 Score = 26.1 bits (56), Expect = 8.0, Method: Composition-based stats. Identities = 9/26 (34%), Positives = 13/26 (50%) Query: 44 FGAAACSIGFAVRYLNAYTGIVLLRC 69 +G A C++ Y N Y I+ L C Sbjct: 272 YGEALCNVLIGFFYANMYCSILFLTC 297 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.139 0.369 Lambda K H 0.267 0.0427 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,134,644 Number of Sequences: 36641 Number of extensions: 222596 Number of successful extensions: 732 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 711 Number of HSP's gapped (non-prelim): 23 length of query: 163 length of database: 10,336,785 effective HSP length: 90 effective length of query: 73 effective length of database: 7,039,095 effective search space: 513853935 effective search space used: 513853935 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 55 (25.7 bits)