BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q95274 (44 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4PL7-A nrPDB 86 4e-18 4PL8-H nrPDB 83 4e-17 3TU5-B nrPDB 56 4e-09 3M3N-W nrPDB 55 1e-08 1T44-G nrPDB 41 1e-04 6NS2-A nrPDB 25 7.4 >4PL7-A nrPDB Length = 440 Score = 85.9 bits (211), Expect = 4e-18, Method: Compositional matrix adjust. Identities = 43/43 (100%), Positives = 43/43 (100%) Query: 2 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 44 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES Sbjct: 390 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 432 >4PL8-H nrPDB Length = 73 Score = 82.8 bits (203), Expect = 4e-17, Method: Compositional matrix adjust. Identities = 43/43 (100%), Positives = 43/43 (100%) Query: 2 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 44 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES Sbjct: 31 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 73 >3TU5-B nrPDB Length = 297 Score = 55.8 bits (133), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 26/29 (89%), Positives = 29/29 (100%) Query: 16 SKLKKTETQEKNPLPSKETIEQEKQAGES 44 ++L+KTETQEKNPLPSKETIEQEKQAGES Sbjct: 269 ARLRKTETQEKNPLPSKETIEQEKQAGES 297 >3M3N-W nrPDB Length = 101 Score = 54.7 bits (130), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 27/28 (96%), Positives = 28/28 (100%) Query: 17 KLKKTETQEKNPLPSKETIEQEKQAGES 44 +LKKTETQEKNPLPSKETIEQEKQAGES Sbjct: 74 QLKKTETQEKNPLPSKETIEQEKQAGES 101 >1T44-G nrPDB Length = 147 Score = 41.2 bits (95), Expect = 1e-04, Method: Composition-based stats. Identities = 21/25 (84%), Positives = 21/25 (84%) Query: 16 SKLKKTETQEKNPLPSKETIEQEKQ 40 S K ETQEKNPLPSKETIEQEKQ Sbjct: 123 SGFKHVETQEKNPLPSKETIEQEKQ 147 >6NS2-A nrPDB Length = 769 Score = 25.0 bits (53), Expect = 7.4, Method: Compositional matrix adjust. Identities = 9/23 (39%), Positives = 16/23 (69%) Query: 5 PDMAEIEKFDKSKLKKTETQEKN 27 P +A++EKF+K++ KT+ N Sbjct: 219 PTLADVEKFNKAQFPKTDGDASN 241 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4PL7-A nrPDB 98 1e-21 3TU5-B nrPDB 62 7e-11 1T44-G nrPDB 56 3e-09 4PL8-H nrPDB 53 4e-08 3M3N-W nrPDB 44 1e-05 Sequences not found previously or not previously below threshold: CONVERGED! >4PL7-A nrPDB Length = 440 Score = 97.6 bits (241), Expect = 1e-21, Method: Composition-based stats. Identities = 43/43 (100%), Positives = 43/43 (100%) Query: 2 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 44 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES Sbjct: 390 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 432 >3TU5-B nrPDB Length = 297 Score = 61.8 bits (148), Expect = 7e-11, Method: Composition-based stats. Identities = 26/29 (89%), Positives = 29/29 (100%) Query: 16 SKLKKTETQEKNPLPSKETIEQEKQAGES 44 ++L+KTETQEKNPLPSKETIEQEKQAGES Sbjct: 269 ARLRKTETQEKNPLPSKETIEQEKQAGES 297 >1T44-G nrPDB Length = 147 Score = 56.4 bits (134), Expect = 3e-09, Method: Composition-based stats. Identities = 21/25 (84%), Positives = 21/25 (84%) Query: 16 SKLKKTETQEKNPLPSKETIEQEKQ 40 S K ETQEKNPLPSKETIEQEKQ Sbjct: 123 SGFKHVETQEKNPLPSKETIEQEKQ 147 >4PL8-H nrPDB Length = 73 Score = 52.6 bits (124), Expect = 4e-08, Method: Composition-based stats. Identities = 43/43 (100%), Positives = 43/43 (100%) Query: 2 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 44 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES Sbjct: 31 SDKPDMAEIEKFDKSKLKKTETQEKNPLPSKETIEQEKQAGES 73 >3M3N-W nrPDB Length = 101 Score = 44.1 bits (102), Expect = 1e-05, Method: Composition-based stats. Identities = 27/28 (96%), Positives = 28/28 (100%) Query: 17 KLKKTETQEKNPLPSKETIEQEKQAGES 44 +LKKTETQEKNPLPSKETIEQEKQAGES Sbjct: 74 QLKKTETQEKNPLPSKETIEQEKQAGES 101 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.287 0.117 0.297 Lambda K H 0.267 0.0371 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 1,395,225 Number of Sequences: 36641 Number of extensions: 29143 Number of successful extensions: 46 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 1 Number of HSP's that attempted gapping in prelim test: 31 Number of HSP's gapped (non-prelim): 15 length of query: 44 length of database: 10,336,785 effective HSP length: 17 effective length of query: 27 effective length of database: 9,713,888 effective search space: 262274976 effective search space used: 262274976 T: 11 A: 40 X1: 16 ( 6.6 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 44 (21.3 bits) S2: 52 (24.8 bits)