BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q94524 (111 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3FM7-A nrPDB 232 2e-62 5WI4-A nrPDB 169 4e-43 5JPW-A nrPDB 164 7e-42 1XDX-A nrPDB 140 1e-34 1J71-A nrPDB 26 3.7 5U2G-A nrPDB 25 7.1 3TW5-A nrPDB 25 8.8 >3FM7-A nrPDB Length = 111 Score = 232 bits (592), Expect = 2e-62, Method: Compositional matrix adjust. Identities = 111/111 (100%), Positives = 111/111 (100%) Query: 1 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK 60 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK Sbjct: 1 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK 60 Query: 61 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV Sbjct: 61 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 >5WI4-A nrPDB Length = 145 Score = 169 bits (427), Expect = 4e-43, Method: Compositional matrix adjust. Identities = 76/113 (67%), Positives = 94/113 (83%), Gaps = 2/113 (1%) Query: 1 MDD--SREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKP 58 M+D + EE+ F+VD+VS +KEAIE+ IGGNAYQH KVN WT V+E L+ LTK +P Sbjct: 3 MEDFQASEETAFVVDEVSSIVKEAIESAIGGNAYQHSKVNQWTTNVLEQTLSQLTKLGRP 62 Query: 59 YKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 +KYIVT +IMQKNGAGLH+ASSC+W++ TDGSCTVRWENKTMYCIVS FGL++ Sbjct: 63 FKYIVTCVIMQKNGAGLHSASSCFWDSSTDGSCTVRWENKTMYCIVSTFGLSI 115 >5JPW-A nrPDB Length = 143 Score = 164 bits (415), Expect = 7e-42, Method: Compositional matrix adjust. Identities = 77/109 (70%), Positives = 90/109 (82%), Gaps = 2/109 (1%) Query: 1 MDD--SREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKP 58 M+D + EE+ F+VD+VS +KEAIE+ IGGNAYQH KVN WT VVE L+ LTK KP Sbjct: 3 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 62 Query: 59 YKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVF 107 +KYIVT +IMQKNGAGLHTASSC+W++ TDGSCTVRWENKTMYCIVS F Sbjct: 63 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAF 111 >1XDX-A nrPDB Length = 114 Score = 140 bits (353), Expect = 1e-34, Method: Compositional matrix adjust. Identities = 62/111 (55%), Positives = 83/111 (74%) Query: 1 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK 60 +D + EE+ F+ DDVS IKE+I+ + Y KV+ WT +E+C+ LT KP+K Sbjct: 4 VDPAVEEAAFVADDVSNIIKESIDAVLQNQQYSEAKVSQWTSSCLEHCIKRLTALNKPFK 63 Query: 61 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 Y+VT +IMQKNGAGLHTA+SC+W++ TDGS TVRWENK+MYCI +VFGLA+ Sbjct: 64 YVVTCIIMQKNGAGLHTAASCWWDSTTDGSRTVRWENKSMYCICTVFGLAI 114 >1J71-A nrPDB Length = 334 Score = 26.2 bits (56), Expect = 3.7, Method: Compositional matrix adjust. Identities = 18/51 (35%), Positives = 21/51 (41%), Gaps = 1/51 (1%) Query: 50 TVLTKEQKPYKY-IVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKT 99 T L E Y IV QK + T SS W DTD C V + +T Sbjct: 5 TTLINEGPSYAADIVVGSNQQKQTVVIDTGSSDLWVVDTDAECQVTYSGQT 55 >5U2G-A nrPDB Length = 838 Score = 25.0 bits (53), Expect = 7.1, Method: Composition-based stats. Identities = 13/40 (32%), Positives = 21/40 (52%), Gaps = 5/40 (12%) Query: 22 AIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYKY 61 AIE +GG +Y+ K N T +V+ ++ KP+ Y Sbjct: 399 AIEAVVGGFSYEQSKFNRATQSLVQVGSSI-----KPFIY 433 >3TW5-A nrPDB Length = 367 Score = 24.6 bits (52), Expect = 8.8, Method: Compositional matrix adjust. Identities = 11/25 (44%), Positives = 17/25 (68%), Gaps = 1/25 (4%) Query: 87 TDGS-CTVRWENKTMYCIVSVFGLA 110 TDGS C++R + YCI + FG++ Sbjct: 104 TDGSSCSIRTGKTSGYCIPTWFGIS 128 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5WI4-A nrPDB 191 8e-50 5JPW-A nrPDB 188 4e-49 3FM7-A nrPDB 187 9e-49 1XDX-A nrPDB 184 6e-48 Sequences not found previously or not previously below threshold: 3TW5-A nrPDB 30 0.32 5U2G-A nrPDB 29 0.44 6MRT-A nrPDB 27 2.5 5ZRV-A nrPDB 26 3.0 6N8J-m nrPDB 26 3.5 5OHS-A nrPDB 25 7.1 3EBY-A nrPDB 25 8.7 6ETZ-A nrPDB 25 9.7 CONVERGED! >5WI4-A nrPDB Length = 145 Score = 191 bits (484), Expect = 8e-50, Method: Composition-based stats. Identities = 76/113 (67%), Positives = 94/113 (83%), Gaps = 2/113 (1%) Query: 1 MDD--SREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKP 58 M+D + EE+ F+VD+VS +KEAIE+ IGGNAYQH KVN WT V+E L+ LTK +P Sbjct: 3 MEDFQASEETAFVVDEVSSIVKEAIESAIGGNAYQHSKVNQWTTNVLEQTLSQLTKLGRP 62 Query: 59 YKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 +KYIVT +IMQKNGAGLH+ASSC+W++ TDGSCTVRWENKTMYCIVS FGL++ Sbjct: 63 FKYIVTCVIMQKNGAGLHSASSCFWDSSTDGSCTVRWENKTMYCIVSTFGLSI 115 >5JPW-A nrPDB Length = 143 Score = 188 bits (478), Expect = 4e-49, Method: Composition-based stats. Identities = 77/109 (70%), Positives = 90/109 (82%), Gaps = 2/109 (1%) Query: 1 MDD--SREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKP 58 M+D + EE+ F+VD+VS +KEAIE+ IGGNAYQH KVN WT VVE L+ LTK KP Sbjct: 3 MEDYQAAEETAFVVDEVSNIVKEAIESAIGGNAYQHSKVNQWTTNVVEQTLSQLTKLGKP 62 Query: 59 YKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVF 107 +KYIVT +IMQKNGAGLHTASSC+W++ TDGSCTVRWENKTMYCIVS F Sbjct: 63 FKYIVTCVIMQKNGAGLHTASSCFWDSSTDGSCTVRWENKTMYCIVSAF 111 >3FM7-A nrPDB Length = 111 Score = 187 bits (475), Expect = 9e-49, Method: Composition-based stats. Identities = 111/111 (100%), Positives = 111/111 (100%) Query: 1 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK 60 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK Sbjct: 1 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK 60 Query: 61 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV Sbjct: 61 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 >1XDX-A nrPDB Length = 114 Score = 184 bits (468), Expect = 6e-48, Method: Composition-based stats. Identities = 62/111 (55%), Positives = 83/111 (74%) Query: 1 MDDSREESQFIVDDVSKTIKEAIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYK 60 +D + EE+ F+ DDVS IKE+I+ + Y KV+ WT +E+C+ LT KP+K Sbjct: 4 VDPAVEEAAFVADDVSNIIKESIDAVLQNQQYSEAKVSQWTSSCLEHCIKRLTALNKPFK 63 Query: 61 YIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWENKTMYCIVSVFGLAV 111 Y+VT +IMQKNGAGLHTA+SC+W++ TDGS TVRWENK+MYCI +VFGLA+ Sbjct: 64 YVVTCIIMQKNGAGLHTAASCWWDSTTDGSRTVRWENKSMYCICTVFGLAI 114 >3TW5-A nrPDB Length = 367 Score = 29.7 bits (65), Expect = 0.32, Method: Composition-based stats. Identities = 22/85 (25%), Positives = 35/85 (41%), Gaps = 7/85 (8%) Query: 32 YQHDKVNNWTGQVVENCLTVLTKEQKPYKYIVTAMIMQKNGAGLHTA-SSCYWNND---- 86 YQ W+ K K + A+ +KNG + C ++D Sbjct: 45 YQDSINVQWSQGQPSAAEKYAKAFGKDVKTFMDAV-SKKNGIDSQSGRKKCSSDDDCSTL 103 Query: 87 TDGS-CTVRWENKTMYCIVSVFGLA 110 TDGS C++R + YCI + FG++ Sbjct: 104 TDGSSCSIRTGKTSGYCIPTWFGIS 128 >5U2G-A nrPDB Length = 838 Score = 29.3 bits (64), Expect = 0.44, Method: Composition-based stats. Identities = 13/40 (32%), Positives = 18/40 (45%), Gaps = 5/40 (12%) Query: 22 AIETTIGGNAYQHDKVNNWTGQVVENCLTVLTKEQKPYKY 61 AIE +GG +Y+ K N T L + KP+ Y Sbjct: 399 AIEAVVGGFSYEQSKFNRAT-----QSLVQVGSSIKPFIY 433 >6MRT-A nrPDB Length = 324 Score = 26.6 bits (57), Expect = 2.5, Method: Composition-based stats. Identities = 16/69 (23%), Positives = 32/69 (46%), Gaps = 7/69 (10%) Query: 3 DSREESQFIVDDVSKTIKEAIETTIGG----NAYQHDKVNNWTGQVVENCLTVLTKEQKP 58 D+ ++ + D + +K AIET G N Y D+V W Q + + L++ ++ Sbjct: 18 DAGSMTEIVADKTVEVVKNAIETADGALDLYNKYL-DQVIPW--QTFDETIKELSRFKQE 74 Query: 59 YKYIVTAMI 67 Y + ++ Sbjct: 75 YSQAASVLV 83 >5ZRV-A nrPDB Length = 763 Score = 26.2 bits (56), Expect = 3.0, Method: Composition-based stats. Identities = 18/64 (28%), Positives = 25/64 (39%) Query: 30 NAYQHDKVNNWTGQVVENCLTVLTKEQKPYKYIVTAMIMQKNGAGLHTASSCYWNNDTDG 89 N K Q+ + ++ K +K K IV A+ G GL A SC + T Sbjct: 105 NMLAACKTLQEVTQLSQEAQRIVEKLEKSTKPIVAAINGSCLGGGLEVAISCQYRIATKD 164 Query: 90 SCTV 93 TV Sbjct: 165 RKTV 168 >6N8J-m nrPDB Length = 486 Score = 26.2 bits (56), Expect = 3.5, Method: Composition-based stats. Identities = 10/32 (31%), Positives = 15/32 (46%) Query: 20 KEAIETTIGGNAYQHDKVNNWTGQVVENCLTV 51 K+ ++ T+G Q DK N WT E + Sbjct: 174 KQVLDATLGLMGNQEDKENGWTSAAKEAIFSK 205 >5OHS-A nrPDB Length = 672 Score = 25.0 bits (53), Expect = 7.1, Method: Composition-based stats. Identities = 9/30 (30%), Positives = 15/30 (50%) Query: 13 DDVSKTIKEAIETTIGGNAYQHDKVNNWTG 42 D + I A+ + + GNAY H + +T Sbjct: 464 DGLVTVICGALSSGLMGNAYHHSDIGGYTS 493 >3EBY-A nrPDB Length = 163 Score = 24.6 bits (52), Expect = 8.7, Method: Composition-based stats. Identities = 9/46 (19%), Positives = 20/46 (43%), Gaps = 2/46 (4%) Query: 11 IVDDVSKTIKEAIE--TTIGGNAYQHDKVNNWTGQVVENCLTVLTK 54 + +V++ + AI+ G D++ W V++CL + Sbjct: 4 VAAEVAQVAQSAIDDFNAAYGLCLDDDRLEQWPTLFVDDCLYQVIA 49 >6ETZ-A nrPDB Length = 989 Score = 24.6 bits (52), Expect = 9.7, Method: Composition-based stats. Identities = 12/58 (20%), Positives = 24/58 (41%), Gaps = 1/58 (1%) Query: 39 NWTGQVVENCLTVLTKEQKPYKYIVTAMIMQKNGAGLHTASSCYWNNDTDGSCTVRWE 96 W +V+ + + K + +V + + G G + A+ W D D S + +E Sbjct: 390 QWEDALVDR-MRRTVERDKNHASVVMWSLGNEAGTGRNLAAMSRWTKDRDPSRPIHYE 446 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.133 0.408 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,501,517 Number of Sequences: 36641 Number of extensions: 156900 Number of successful extensions: 388 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 21 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 367 Number of HSP's gapped (non-prelim): 34 length of query: 111 length of database: 10,336,785 effective HSP length: 77 effective length of query: 34 effective length of database: 7,515,428 effective search space: 255524552 effective search space used: 255524552 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 52 (24.6 bits)