BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q93099 (445 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1EYB-A nrPDB 931 0.0 3ZDS-A nrPDB 426 e-120 1F2V-A nrPDB 32 0.64 2Z0Q-A nrPDB 29 3.7 >1EYB-A nrPDB Length = 471 Score = 931 bits (2405), Expect = 0.0, Method: Compositional matrix adjust. Identities = 444/445 (99%), Positives = 444/445 (99%) Query: 1 MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSW 60 MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSW Sbjct: 27 MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSW 86 Query: 61 LYRILPSVSHKPFESIDEGQVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLC 120 LYRILPSVSHKPFESIDEG VTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLC Sbjct: 87 LYRILPSVSHKPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLC 146 Query: 121 GAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEIC 180 GAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEIC Sbjct: 147 GAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEIC 206 Query: 181 VIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVP 240 VIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVP Sbjct: 207 VIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVP 266 Query: 241 GGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTV 300 GGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTV Sbjct: 267 GGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTV 326 Query: 301 LTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPG 360 LTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPG Sbjct: 327 LTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPG 386 Query: 361 GGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLD 420 GGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLD Sbjct: 387 GGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLD 446 Query: 421 ENYHKCWEPLKSHFTPNSRNPAEPN 445 ENYHKCWEPLKSHFTPNSRNPAEPN Sbjct: 447 ENYHKCWEPLKSHFTPNSRNPAEPN 471 >3ZDS-A nrPDB Length = 433 Score = 426 bits (1095), Expect = e-120, Method: Compositional matrix adjust. Identities = 224/440 (50%), Positives = 290/440 (65%), Gaps = 17/440 (3%) Query: 3 ELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLY 62 +L Y+SGFGNE +SE PG+LP GQN+PQ PY LYAE LSG+AFT RS +R+WLY Sbjct: 8 DLHYLSGFGNEFASEA--LPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSELRRTWLY 65 Query: 63 RILPSVSHKPFESIDEGQVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGA 122 RI PS H FE + + ++P N+LRW P IP + DF+ G + Sbjct: 66 RIRPSALHPRFERLARQPLGGPLGGINP--NRLRWSPQPIPA---EPTDFIEGWLPMAAN 120 Query: 123 GDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVI 182 + G++I+I+ N SME R F+N+DG+ L+VP++G L I TE G M V+P EI VI Sbjct: 121 AGAEKPAGVSIYIYRANRSME-RVFFNADGELLLVPEQGRLRIATELGVMEVEPLEIAVI 179 Query: 183 QRGMRFSIDVFE-ETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVPG 241 RGM+F +++ + + RGYI E +G LPDLGPIG+NGLANPRDFL P+A YE+ + P Sbjct: 180 PRGMKFRVELLDGQARGYIAENHGAPLRLPDLGPIGSNGLANPRDFLTPVAHYEEAEGP- 238 Query: 242 GYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTVL 301 ++ K+ G+ +A + SP +VVAWHG+ PYKY+L+ F I +V+FDH DPSIFTVL Sbjct: 239 -VQLVQKFLGEHWACELQHSPLDVVAWHGSNVPYKYDLRRFNTIGTVSFDHPDPSIFTVL 297 Query: 302 TAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPGG 361 T+ + G+A DFVIFPPRW VA+ TFRPP++HRN M+EFMGLI G Y+AK GFLPGG Sbjct: 298 TSPTSVHGMANMDFVIFPPRWMVAENTFRPPWFHRNLMNEFMGLINGAYDAKAEGFLPGG 357 Query: 362 GSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRC--L 419 SLH M+ HGPDA+ EKA LAP +I D TMAFMFE+S V + L+A C L Sbjct: 358 ASLHGVMSAHGPDAETCEKAIAADLAPHKI-DNTMAFMFETS---QVLRPSLQALECPQL 413 Query: 420 DENYHKCWEPLKSHFTPNSR 439 +Y CW L S F PN R Sbjct: 414 QADYDSCWATLPSTFNPNRR 433 >1F2V-A nrPDB Length = 219 Score = 31.6 bits (70), Expect = 0.64, Method: Compositional matrix adjust. Identities = 19/64 (29%), Positives = 28/64 (43%), Gaps = 2/64 (3%) Query: 11 GNE--CSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLYRILPSV 68 GNE C+ DPR P E N L++E+L+GS + +L +L Sbjct: 102 GNEVICTLRDPRTPALAAEIGNTRSAAALKLWSERLAGSVVAIGNAPTALFFLLEMLRDG 161 Query: 69 SHKP 72 + KP Sbjct: 162 APKP 165 >2Z0Q-A nrPDB Length = 352 Score = 29.3 bits (64), Expect = 3.7, Method: Compositional matrix adjust. Identities = 10/30 (33%), Positives = 18/30 (60%) Query: 108 KKVDFVSGLHTLCGAGDIKSNNGLAIHIFL 137 +K + LC G++K+N G+ +H+FL Sbjct: 221 QKDSLIDSSRVLCCHGELKNNRGVKLHVFL 250 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1EYB-A nrPDB 903 0.0 3ZDS-A nrPDB 727 0.0 Sequences not found previously or not previously below threshold: 4QQR-A nrPDB 31 0.96 4E8C-A nrPDB 30 1.4 5UDG-A nrPDB 30 1.5 4F8B-A nrPDB 30 1.6 4E19-A nrPDB 30 2.2 CONVERGED! >1EYB-A nrPDB Length = 471 Score = 903 bits (2334), Expect = 0.0, Method: Composition-based stats. Identities = 444/445 (99%), Positives = 444/445 (99%) Query: 1 MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSW 60 MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSW Sbjct: 27 MAELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSW 86 Query: 61 LYRILPSVSHKPFESIDEGQVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLC 120 LYRILPSVSHKPFESIDEG VTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLC Sbjct: 87 LYRILPSVSHKPFESIDEGHVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLC 146 Query: 121 GAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEIC 180 GAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEIC Sbjct: 147 GAGDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEIC 206 Query: 181 VIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVP 240 VIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVP Sbjct: 207 VIQRGMRFSIDVFEETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVP 266 Query: 241 GGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTV 300 GGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTV Sbjct: 267 GGYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTV 326 Query: 301 LTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPG 360 LTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPG Sbjct: 327 LTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPG 386 Query: 361 GGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLD 420 GGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLD Sbjct: 387 GGSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRCLD 446 Query: 421 ENYHKCWEPLKSHFTPNSRNPAEPN 445 ENYHKCWEPLKSHFTPNSRNPAEPN Sbjct: 447 ENYHKCWEPLKSHFTPNSRNPAEPN 471 >3ZDS-A nrPDB Length = 433 Score = 727 bits (1878), Expect = 0.0, Method: Composition-based stats. Identities = 224/440 (50%), Positives = 290/440 (65%), Gaps = 17/440 (3%) Query: 3 ELKYISGFGNECSSEDPRCPGSLPEGQNNPQVCPYNLYAEQLSGSAFTCPRSTNKRSWLY 62 +L Y+SGFGNE +SE PG+LP GQN+PQ PY LYAE LSG+AFT RS +R+WLY Sbjct: 8 DLHYLSGFGNEFASEA--LPGALPVGQNSPQKAPYGLYAELLSGTAFTMARSELRRTWLY 65 Query: 63 RILPSVSHKPFESIDEGQVTHNWDEVDPDPNQLRWKPFEIPKASQKKVDFVSGLHTLCGA 122 RI PS H FE + + ++P N+LRW P IP + DF+ G + Sbjct: 66 RIRPSALHPRFERLARQPLGGPLGGINP--NRLRWSPQPIPA---EPTDFIEGWLPMAAN 120 Query: 123 GDIKSNNGLAIHIFLCNTSMENRCFYNSDGDFLIVPQKGNLLIYTEFGKMLVQPNEICVI 182 + G++I+I+ N SME R F+N+DG+ L+VP++G L I TE G M V+P EI VI Sbjct: 121 AGAEKPAGVSIYIYRANRSME-RVFFNADGELLLVPEQGRLRIATELGVMEVEPLEIAVI 179 Query: 183 QRGMRFSIDVFE-ETRGYILEVYGVHFELPDLGPIGANGLANPRDFLIPIAWYEDRQVPG 241 RGM+F +++ + + RGYI E +G LPDLGPIG+NGLANPRDFL P+A YE+ + P Sbjct: 180 PRGMKFRVELLDGQARGYIAENHGAPLRLPDLGPIGSNGLANPRDFLTPVAHYEEAEGP- 238 Query: 242 GYTVINKYQGKLFAAKQDVSPFNVVAWHGNYTPYKYNLKNFMVINSVAFDHADPSIFTVL 301 ++ K+ G+ +A + SP +VVAWHG+ PYKY+L+ F I +V+FDH DPSIFTVL Sbjct: 239 -VQLVQKFLGEHWACELQHSPLDVVAWHGSNVPYKYDLRRFNTIGTVSFDHPDPSIFTVL 297 Query: 302 TAKSVRPGVAIADFVIFPPRWGVADKTFRPPYYHRNCMSEFMGLIRGHYEAKQGGFLPGG 361 T+ + G+A DFVIFPPRW VA+ TFRPP++HRN M+EFMGLI G Y+AK GFLPGG Sbjct: 298 TSPTSVHGMANMDFVIFPPRWMVAENTFRPPWFHRNLMNEFMGLINGAYDAKAEGFLPGG 357 Query: 362 GSLHSTMTPHGPDADCFEKASKVKLAPERIADGTMAFMFESSLSLAVTKWGLKASRC--L 419 SLH M+ HGPDA+ EKA LAP +I D TMAFMFE+S V + L+A C L Sbjct: 358 ASLHGVMSAHGPDAETCEKAIAADLAPHKI-DNTMAFMFETS---QVLRPSLQALECPQL 413 Query: 420 DENYHKCWEPLKSHFTPNSR 439 +Y CW L S F PN R Sbjct: 414 QADYDSCWATLPSTFNPNRR 433 >4QQR-A nrPDB Length = 301 Score = 31.2 bits (69), Expect = 0.96, Method: Composition-based stats. Identities = 13/37 (35%), Positives = 22/37 (59%), Gaps = 2/37 (5%) Query: 215 PIGANGLANPRDFLIPIAWYED-RQVPGGYTVINKYQ 250 PI ++ L NPR+F+ IA YE +P T++++ Sbjct: 169 PISSD-LTNPRNFITKIARYEKVVDIPNSMTILDELL 204 >4E8C-A nrPDB Length = 595 Score = 30.4 bits (67), Expect = 1.4, Method: Composition-based stats. Identities = 8/22 (36%), Positives = 13/22 (59%) Query: 95 LRWKPFEIPKASQKKVDFVSGL 116 L WK + +P + +K+DF G Sbjct: 480 LNWKHYPLPLDNPEKIDFSKGW 501 >5UDG-A nrPDB Length = 145 Score = 30.4 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 27/121 (22%), Positives = 46/121 (38%), Gaps = 15/121 (12%) Query: 288 VAFDHADPSIFTVLTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYY--------HRNCM 339 V F+ + FT L K+ +P A P V K+ + + H++CM Sbjct: 25 VKFNCPE---FTSLCPKTGQPDFATIYISYIPDEKMVESKSLKLYLFSFRNHGDFHQDCM 81 Query: 340 SEFMG----LIRGHYEAKQGGFLPGGGSLHSTMTPHGPDADCFEKASKVKLAPERIADGT 395 + M L+ Y G F P GG T +G +EK ++ ++ + T Sbjct: 82 NIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPET 141 Query: 396 M 396 + Sbjct: 142 I 142 >4F8B-A nrPDB Length = 165 Score = 30.4 bits (67), Expect = 1.6, Method: Composition-based stats. Identities = 27/121 (22%), Positives = 45/121 (37%), Gaps = 15/121 (12%) Query: 288 VAFDHADPSIFTVLTAKSVRPGVAIADFVIFPPRWGVADKTFRPPYY--------HRNCM 339 V F+ + FT L K+ +P A P V K+ + + H +CM Sbjct: 45 VKFNCPE---FTSLCPKTGQPDFATIYISYIPDEKMVESKSLKLYLFSFRNHGDFHEDCM 101 Query: 340 SEFMG----LIRGHYEAKQGGFLPGGGSLHSTMTPHGPDADCFEKASKVKLAPERIADGT 395 + M L+ Y G F P GG T +G +EK ++ ++ + T Sbjct: 102 NIIMNDLIELMDPRYIEVWGKFTPRGGISIDPYTNYGKPGTKYEKMAEYRMMNHDLYPET 161 Query: 396 M 396 + Sbjct: 162 I 162 >4E19-A nrPDB Length = 199 Score = 30.0 bits (66), Expect = 2.2, Method: Composition-based stats. Identities = 13/49 (26%), Positives = 21/49 (42%), Gaps = 12/49 (24%) Query: 75 SIDEGQVTHNWDEVDPDPNQLR------------WKPFEIPKASQKKVD 111 + E Q+T WD DPD + R W +P+A+ ++ D Sbjct: 141 QLVEKQLTGAWDTNDPDLRRKRVRARELLTGFDDWSITHVPRATNERAD 189 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.135 0.419 Lambda K H 0.267 0.0404 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 19,966,950 Number of Sequences: 36641 Number of extensions: 855004 Number of successful extensions: 1675 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 1651 Number of HSP's gapped (non-prelim): 13 length of query: 445 length of database: 10,336,785 effective HSP length: 100 effective length of query: 345 effective length of database: 6,672,685 effective search space: 2302076325 effective search space used: 2302076325 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.0 bits) S2: 61 (28.1 bits)