BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q92990 (594 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4F52-E nrPDB 1202 0.0 1JC9-A nrPDB 32 0.63 1R5O-A nrPDB 31 1.4 4M75-A nrPDB 30 3.0 4C8Q-A nrPDB 30 4.1 1QWG-A nrPDB 29 5.0 4L22-A nrPDB 29 6.4 >4F52-E nrPDB Length = 596 Score = 1202 bits (3109), Expect = 0.0, Method: Compositional matrix adjust. Identities = 594/594 (100%), Positives = 594/594 (100%) Query: 1 MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKN 60 MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKN Sbjct: 3 MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKN 62 Query: 61 MGWNLVGPVVRCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQ 120 MGWNLVGPVVRCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQ Sbjct: 63 MGWNLVGPVVRCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQ 122 Query: 121 SILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQCCKAL 180 SILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQCCKAL Sbjct: 123 SILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQCCKAL 182 Query: 181 IEFTKPFVEEVIDNKENSLENEKLKDELLKFCFKSLKCPLLTAQFFEQSEEGGNDPFRYF 240 IEFTKPFVEEVIDNKENSLENEKLKDELLKFCFKSLKCPLLTAQFFEQSEEGGNDPFRYF Sbjct: 183 IEFTKPFVEEVIDNKENSLENEKLKDELLKFCFKSLKCPLLTAQFFEQSEEGGNDPFRYF 242 Query: 241 ASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHI 300 ASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHI Sbjct: 243 ASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHI 302 Query: 301 DQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKS 360 DQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKS Sbjct: 303 DQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKS 362 Query: 361 FLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFI 420 FLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFI Sbjct: 363 FLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFI 422 Query: 421 IQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRY 480 IQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRY Sbjct: 423 IQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRY 482 Query: 481 LVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSIT 540 LVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSIT Sbjct: 483 LVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSIT 542 Query: 541 VSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK 594 VSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK Sbjct: 543 VSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK 596 >1JC9-A nrPDB Length = 269 Score = 32.3 bits (72), Expect = 0.63, Method: Compositional matrix adjust. Identities = 25/83 (30%), Positives = 39/83 (46%), Gaps = 11/83 (13%) Query: 461 ETDLLQNSDRIMASLNLLRYLV---IKDNENDNQTGLWTELGNIEN-NFLKPLHIGLNMS 516 E D +DRI A N Y++ +KD END + ++ + IEN ++L LHIG Sbjct: 120 EKDFWLGNDRIFALTNQRNYMIRFDLKDKENDTRYAIYQDFW-IENEDYLYCLHIG---- 174 Query: 517 KAHYEAEIKNSQEAQKSKDLCSI 539 +Y + NS + +I Sbjct: 175 --NYSGDAGNSFGRHNGHNFSTI 195 >1R5O-A nrPDB Length = 467 Score = 31.2 bits (69), Expect = 1.4, Method: Compositional matrix adjust. Identities = 14/41 (34%), Positives = 23/41 (56%), Gaps = 5/41 (12%) Query: 416 VEAFIIQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFL 456 V A+ QN+K+++D S+ W+ GP L+ LD + L Sbjct: 231 VSAYTGQNVKDRVDSSV-----CPWYQGPSLLEYLDSMTHL 266 >4M75-A nrPDB Length = 144 Score = 30.0 bits (66), Expect = 3.0, Method: Composition-based stats. Identities = 13/38 (34%), Positives = 20/38 (52%) Query: 6 LQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTD 43 LQ ++R EE + EED G+F + G+ + G D Sbjct: 51 LQDCVERIYFSEENKYAEEDRGIFMIRGENVVMLGEVD 88 >4C8Q-A nrPDB Length = 101 Score = 29.6 bits (65), Expect = 4.1, Method: Composition-based stats. Identities = 13/38 (34%), Positives = 20/38 (52%) Query: 6 LQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTD 43 LQ ++R EE + EED G+F + G+ + G D Sbjct: 35 LQDCVERIYFSEENKYAEEDRGIFMIRGENVVMLGEVD 72 >1QWG-A nrPDB Length = 251 Score = 29.3 bits (64), Expect = 5.0, Method: Compositional matrix adjust. Identities = 36/143 (25%), Positives = 68/143 (47%), Gaps = 20/143 (13%) Query: 397 SQGKYTLFRCLLNTSNHSGVEAFIIQNIKNQIDM-----SLKRTRNNKWFTGPQLISLLD 451 S+GK+ F LN G EA I + + I + ++KR ++N + L + Sbjct: 83 SKGKFDEF---LNECEKLGFEAVEISDGSSDISLEERNNAIKRAKDNGFMV------LTE 133 Query: 452 LVLFLPEGAETDLLQNSDRI-MASLNL---LRYLVIKDNENDNQTGLWTELGNIENNFLK 507 + +P+ + L DRI + + +L Y++I+ E+ GL+ + G ++ N L Sbjct: 134 VGKKMPD--KDKQLTIDDRIKLINFDLDAGADYVIIEGRESGKGKGLFDKEGKVKENELD 191 Query: 508 PLHIGLNMSKAHYEAEIKNSQEA 530 L ++++K +EA K+ Q A Sbjct: 192 VLAKNVDINKVIFEAPQKSQQVA 214 >4L22-A nrPDB Length = 758 Score = 28.9 bits (63), Expect = 6.4, Method: Compositional matrix adjust. Identities = 20/55 (36%), Positives = 33/55 (60%), Gaps = 4/55 (7%) Query: 78 KEDSKRKVYFLIFDLLV-KLCNPKELLLGLLELIEE---PSGKQISQSILLLLQP 128 K SKRKVY++ + L+ KL + + LGL + +++ +GK ISQ + L+P Sbjct: 45 KNTSKRKVYYISAEFLIGKLLSNNLINLGLYKTVKDELAAAGKSISQVEDVELEP 99 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4F52-E nrPDB 1211 0.0 Sequences not found previously or not previously below threshold: 1JC9-A nrPDB 33 0.31 4P1X-A nrPDB 31 1.5 4M75-A nrPDB 31 1.5 4C8Q-A nrPDB 31 1.6 1QWG-A nrPDB 31 1.7 3H32-B nrPDB 30 2.7 4L22-A nrPDB 30 4.4 1R5O-A nrPDB 29 5.5 5A0Z-A nrPDB 29 6.6 3KZ4-A nrPDB 28 8.0 6OJ5-A nrPDB 28 8.3 CONVERGED! >4F52-E nrPDB Length = 596 Score = 1211 bits (3133), Expect = 0.0, Method: Composition-based stats. Identities = 594/594 (100%), Positives = 594/594 (100%) Query: 1 MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKN 60 MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKN Sbjct: 3 MAVEELQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKN 62 Query: 61 MGWNLVGPVVRCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQ 120 MGWNLVGPVVRCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQ Sbjct: 63 MGWNLVGPVVRCLLCKDKEDSKRKVYFLIFDLLVKLCNPKELLLGLLELIEEPSGKQISQ 122 Query: 121 SILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQCCKAL 180 SILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQCCKAL Sbjct: 123 SILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDDYGLCQCCKAL 182 Query: 181 IEFTKPFVEEVIDNKENSLENEKLKDELLKFCFKSLKCPLLTAQFFEQSEEGGNDPFRYF 240 IEFTKPFVEEVIDNKENSLENEKLKDELLKFCFKSLKCPLLTAQFFEQSEEGGNDPFRYF Sbjct: 183 IEFTKPFVEEVIDNKENSLENEKLKDELLKFCFKSLKCPLLTAQFFEQSEEGGNDPFRYF 242 Query: 241 ASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHI 300 ASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHI Sbjct: 243 ASEIIGFLSAIGHPFPKMIFNHGRKKRTWNYLEFEEEENKQLADSMASLAYLVFVQGIHI 302 Query: 301 DQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKS 360 DQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKS Sbjct: 303 DQLPMVLSPLYLLQFNMGHIEVFLQRTEESVISKGLELLENSLLRIEDNSLLYQYLEIKS 362 Query: 361 FLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFI 420 FLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFI Sbjct: 363 FLTVPQGLVKVMTLCPIETLRKKSLAMLQLYINKLDSQGKYTLFRCLLNTSNHSGVEAFI 422 Query: 421 IQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRY 480 IQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRY Sbjct: 423 IQNIKNQIDMSLKRTRNNKWFTGPQLISLLDLVLFLPEGAETDLLQNSDRIMASLNLLRY 482 Query: 481 LVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSIT 540 LVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSIT Sbjct: 483 LVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHYEAEIKNSQEAQKSKDLCSIT 542 Query: 541 VSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK 594 VSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK Sbjct: 543 VSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEELIEIKTKSTSEENIGIK 596 >1JC9-A nrPDB Length = 269 Score = 33.5 bits (75), Expect = 0.31, Method: Composition-based stats. Identities = 18/55 (32%), Positives = 28/55 (50%), Gaps = 3/55 (5%) Query: 461 ETDLLQNSDRIMASLNLLRYLV---IKDNENDNQTGLWTELGNIENNFLKPLHIG 512 E D +DRI A N Y++ +KD END + ++ + ++L LHIG Sbjct: 120 EKDFWLGNDRIFALTNQRNYMIRFDLKDKENDTRYAIYQDFWIENEDYLYCLHIG 174 >4P1X-A nrPDB Length = 309 Score = 31.2 bits (69), Expect = 1.5, Method: Composition-based stats. Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 4/96 (4%) Query: 461 ETDLLQNSDRIMASLNLLRYLVIKDNENDNQTGLWTELGNIENNFLKPLHIGLNMSKAHY 520 +T +SD+ S +L + IKD D T + GNI + F+KP + SK ++ Sbjct: 30 KTTATADSDKFKIS-QILTFNFIKDKSYDKDTLVLKATGNINSGFVKPNPNDYDFSKLYW 88 Query: 521 EAEIKNSQEAQKSKDLCSITVSGEEIPNMPPEMQLK 556 A+ S +Q + S+ V N E Q++ Sbjct: 89 GAKYNVSISSQSND---SVNVVDYAPKNQNEEFQVQ 121 >4M75-A nrPDB Length = 144 Score = 31.2 bits (69), Expect = 1.5, Method: Composition-based stats. Identities = 23/78 (29%), Positives = 31/78 (39%), Gaps = 10/78 (12%) Query: 6 LQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTDQLLEIIQNEKNKVIIKNMGWNL 65 LQ ++R EE + EED G+F + G+ + G D E E + I W Sbjct: 51 LQDCVERIYFSEENKYAEEDRGIFMIRGENVVMLGEVDIDKEDQPLEAMERIPFKEAW-- 108 Query: 66 VGPVVRCLLCKDKEDSKR 83 L K K D KR Sbjct: 109 --------LTKQKNDEKR 118 >4C8Q-A nrPDB Length = 101 Score = 30.8 bits (68), Expect = 1.6, Method: Composition-based stats. Identities = 13/38 (34%), Positives = 20/38 (52%) Query: 6 LQSIIKRCQILEEQDFKEEDFGLFQLAGQRCIEEGHTD 43 LQ ++R EE + EED G+F + G+ + G D Sbjct: 35 LQDCVERIYFSEENKYAEEDRGIFMIRGENVVMLGEVD 72 >1QWG-A nrPDB Length = 251 Score = 30.8 bits (68), Expect = 1.7, Method: Composition-based stats. Identities = 36/143 (25%), Positives = 68/143 (47%), Gaps = 20/143 (13%) Query: 397 SQGKYTLFRCLLNTSNHSGVEAFIIQNIKNQIDM-----SLKRTRNNKWFTGPQLISLLD 451 S+GK+ F LN G EA I + + I + ++KR ++N + L + Sbjct: 83 SKGKFDEF---LNECEKLGFEAVEISDGSSDISLEERNNAIKRAKDNGFMV------LTE 133 Query: 452 LVLFLPEGAETDLLQNSDRI-MASLNL---LRYLVIKDNENDNQTGLWTELGNIENNFLK 507 + +P+ + L DRI + + +L Y++I+ E+ GL+ + G ++ N L Sbjct: 134 VGKKMPD--KDKQLTIDDRIKLINFDLDAGADYVIIEGRESGKGKGLFDKEGKVKENELD 191 Query: 508 PLHIGLNMSKAHYEAEIKNSQEA 530 L ++++K +EA K+ Q A Sbjct: 192 VLAKNVDINKVIFEAPQKSQQVA 214 >3H32-B nrPDB Length = 458 Score = 30.0 bits (66), Expect = 2.7, Method: Composition-based stats. Identities = 14/58 (24%), Positives = 32/58 (55%) Query: 113 PSGKQISQSILLLLQPLQTVIQKLHNKAYSIGLALSTLWNQLSLLPVPYSKEQIQMDD 170 P+G Q+ +++L +P++ + +L+N ++ S+ + + LL + K Q Q+ D Sbjct: 77 PTGCQLQEALLQQERPIRNSVDELNNNVEAVSQTSSSSFQYMYLLKDLWQKRQKQVKD 134 >4L22-A nrPDB Length = 758 Score = 29.6 bits (65), Expect = 4.4, Method: Composition-based stats. Identities = 20/55 (36%), Positives = 33/55 (60%), Gaps = 4/55 (7%) Query: 78 KEDSKRKVYFLIFDLLV-KLCNPKELLLGLLELIEE---PSGKQISQSILLLLQP 128 K SKRKVY++ + L+ KL + + LGL + +++ +GK ISQ + L+P Sbjct: 45 KNTSKRKVYYISAEFLIGKLLSNNLINLGLYKTVKDELAAAGKSISQVEDVELEP 99 >1R5O-A nrPDB Length = 467 Score = 29.2 bits (64), Expect = 5.5, Method: Composition-based stats. Identities = 13/36 (36%), Positives = 21/36 (58%), Gaps = 5/36 (13%) Query: 416 VEAFIIQNIKNQIDMSLKRTRNNKWFTGPQLISLLD 451 V A+ QN+K+++D S+ W+ GP L+ LD Sbjct: 231 VSAYTGQNVKDRVDSSV-----CPWYQGPSLLEYLD 261 >5A0Z-A nrPDB Length = 792 Score = 28.8 bits (63), Expect = 6.6, Method: Composition-based stats. Identities = 20/60 (33%), Positives = 27/60 (45%), Gaps = 5/60 (8%) Query: 518 AHYEAEIKNSQEAQKSKDLCSITVSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEE 577 AH Q AQ+ +L +I E +P PP K L AL + +ES L +EE Sbjct: 223 AHARELAAKEQNAQRRAELLTIAEVNENVPANPP----KTLQEALQSIWTVES-LFEIEE 277 >3KZ4-A nrPDB Length = 880 Score = 28.5 bits (62), Expect = 8.0, Method: Composition-based stats. Identities = 19/63 (30%), Positives = 34/63 (53%), Gaps = 8/63 (12%) Query: 521 EAEIKNSQEAQKS-----KDLCSITVSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARV 575 + EIK + E +KS K L + + EE E+Q ++L + TF+ ES+L ++ Sbjct: 52 QEEIKIADEVKKSTKEESKQLLEVLKTKEE---HQKEIQYEILQKTIPTFEPKESILKKL 108 Query: 576 EEL 578 E++ Sbjct: 109 EDI 111 >6OJ5-A nrPDB Length = 887 Score = 28.5 bits (62), Expect = 8.3, Method: Composition-based stats. Identities = 17/56 (30%), Positives = 33/56 (58%), Gaps = 4/56 (7%) Query: 523 EIKNSQEAQKSKDLCSITVSGEEIPNMPPEMQLKVLHSALFTFDLIESVLARVEEL 578 E+K S + ++SK L + + EE E+Q ++L + TF+ ES+L ++E++ Sbjct: 67 EVKKSNK-EESKQLLEVLKTKEE---HQKEVQYEILQKTIPTFEPKESILKKLEDI 118 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.319 0.137 0.393 Lambda K H 0.267 0.0447 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 25,993,806 Number of Sequences: 36641 Number of extensions: 1204326 Number of successful extensions: 2741 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 2725 Number of HSP's gapped (non-prelim): 30 length of query: 594 length of database: 10,336,785 effective HSP length: 103 effective length of query: 491 effective length of database: 6,562,762 effective search space: 3222316142 effective search space used: 3222316142 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 62 (28.4 bits)