BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q91VS7 (155 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5IA9-A nrPDB 311 7e-86 5BQH-A nrPDB 100 2e-22 4BPM-A nrPDB 98 1e-21 3QXY-A nrPDB 30 0.55 4XGC-E nrPDB 29 0.96 >5IA9-A nrPDB Length = 155 Score = 311 bits (797), Expect = 7e-86, Method: Compositional matrix adjust. Identities = 149/155 (96%), Positives = 154/155 (99%) Query: 1 MADLRQLMDNEVLMAFTSYATIILTKMMFMSSATAFQRITNKVFANPEDCAGFGKGENAK 60 MADL+QLMDNEVLMAFTSYATIIL KMMF+SSATAFQR+TNKVFANPEDCAGFGKGENAK Sbjct: 1 MADLKQLMDNEVLMAFTSYATIILAKMMFLSSATAFQRLTNKVFANPEDCAGFGKGENAK 60 Query: 61 KFVRTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALMHFRIFVGARIYHTIA 120 KF+RTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTAL+HFRIFVGARIYHTIA Sbjct: 61 KFLRTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALIHFRIFVGARIYHTIA 120 Query: 121 YLTPLPQPNRGLAFFVGYGVTLSMAYRLLRSRLYL 155 YLTPLPQPNRGLAFFVGYGVTLSMAYRLLRSRLYL Sbjct: 121 YLTPLPQPNRGLAFFVGYGVTLSMAYRLLRSRLYL 155 >5BQH-A nrPDB Length = 154 Score = 100 bits (250), Expect = 2e-22, Method: Compositional matrix adjust. Identities = 55/143 (38%), Positives = 80/143 (55%), Gaps = 4/143 (2%) Query: 7 LMDNEVLMAFTSYATIILTKMMFMSSATAFQRITNKVFANPEDCAGFGKGENAKKFVRTD 66 +M + L AF +T+++ KM ++ T R+ K FANPED G ++ R+D Sbjct: 9 VMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHG----GPQYCRSD 64 Query: 67 EKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALMHFRIFVGARIYHTIAYLTPLP 126 VER RAH ND+E I PFL +G +YS GP+ A MHF +F+ R+ HT+AYL L Sbjct: 65 PDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLR 124 Query: 127 QPNRGLAFFVGYGVTLSMAYRLL 149 P R + + + SMA ++L Sbjct: 125 APIRSVTYTLAQLPCASMALQIL 147 >4BPM-A nrPDB Length = 178 Score = 98.2 bits (243), Expect = 1e-21, Method: Compositional matrix adjust. Identities = 54/140 (38%), Positives = 78/140 (55%), Gaps = 4/140 (2%) Query: 10 NEVLMAFTSYATIILTKMMFMSSATAFQRITNKVFANPEDCAGFGKGENAKKFVRTDEKV 69 + L AF +T+++ KM ++ T R+ K FANPED G ++ R+D V Sbjct: 8 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHG----GPQYCRSDPDV 63 Query: 70 ERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALMHFRIFVGARIYHTIAYLTPLPQPN 129 ER RAH ND+E I PFL +G +YS GP+ A MHF +F+ R+ HT+AYL L P Sbjct: 64 ERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPI 123 Query: 130 RGLAFFVGYGVTLSMAYRLL 149 R + + + SMA ++L Sbjct: 124 RSVTYTLAQLPCASMALQIL 143 >3QXY-A nrPDB Length = 449 Score = 29.6 bits (65), Expect = 0.55, Method: Compositional matrix adjust. Identities = 12/39 (30%), Positives = 22/39 (56%) Query: 2 ADLRQLMDNEVLMAFTSYATIILTKMMFMSSATAFQRIT 40 A RQL+ N VL+ +YAT + T +S+ + +++ Sbjct: 386 ASWRQLLQNSVLLTLQTYATDLKTDQGLLSNKEVYAKLS 424 >4XGC-E nrPDB Length = 460 Score = 28.9 bits (63), Expect = 0.96, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 26/54 (48%) Query: 51 AGFGKGENAKKFVRTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTAL 104 +G GK + F++ K + VR AHLN +E + + +L PD AL Sbjct: 43 SGTGKTALTRAFLKECGKRQNVRTAHLNAIECYTTKIMLEILLDSLAPDQGDAL 96 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5IA9-A nrPDB 256 4e-69 4BPM-A nrPDB 214 1e-56 5BQH-A nrPDB 213 3e-56 Sequences not found previously or not previously below threshold: 3NAW-A nrPDB 26 5.8 5CO4-B nrPDB 26 6.6 5TEY-A nrPDB 26 6.7 3ODM-A nrPDB 26 7.4 CONVERGED! >5IA9-A nrPDB Length = 155 Score = 256 bits (653), Expect = 4e-69, Method: Composition-based stats. Identities = 149/155 (96%), Positives = 154/155 (99%) Query: 1 MADLRQLMDNEVLMAFTSYATIILTKMMFMSSATAFQRITNKVFANPEDCAGFGKGENAK 60 MADL+QLMDNEVLMAFTSYATIIL KMMF+SSATAFQR+TNKVFANPEDCAGFGKGENAK Sbjct: 1 MADLKQLMDNEVLMAFTSYATIILAKMMFLSSATAFQRLTNKVFANPEDCAGFGKGENAK 60 Query: 61 KFVRTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALMHFRIFVGARIYHTIA 120 KF+RTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTAL+HFRIFVGARIYHTIA Sbjct: 61 KFLRTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALIHFRIFVGARIYHTIA 120 Query: 121 YLTPLPQPNRGLAFFVGYGVTLSMAYRLLRSRLYL 155 YLTPLPQPNRGLAFFVGYGVTLSMAYRLLRSRLYL Sbjct: 121 YLTPLPQPNRGLAFFVGYGVTLSMAYRLLRSRLYL 155 >4BPM-A nrPDB Length = 178 Score = 214 bits (546), Expect = 1e-56, Method: Composition-based stats. Identities = 54/140 (38%), Positives = 78/140 (55%), Gaps = 4/140 (2%) Query: 10 NEVLMAFTSYATIILTKMMFMSSATAFQRITNKVFANPEDCAGFGKGENAKKFVRTDEKV 69 + L AF +T+++ KM ++ T R+ K FANPED G ++ R+D V Sbjct: 8 SPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHG----GPQYCRSDPDV 63 Query: 70 ERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALMHFRIFVGARIYHTIAYLTPLPQPN 129 ER RAH ND+E I PFL +G +YS GP+ A MHF +F+ R+ HT+AYL L P Sbjct: 64 ERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLRAPI 123 Query: 130 RGLAFFVGYGVTLSMAYRLL 149 R + + + SMA ++L Sbjct: 124 RSVTYTLAQLPCASMALQIL 143 >5BQH-A nrPDB Length = 154 Score = 213 bits (542), Expect = 3e-56, Method: Composition-based stats. Identities = 55/143 (38%), Positives = 80/143 (55%), Gaps = 4/143 (2%) Query: 7 LMDNEVLMAFTSYATIILTKMMFMSSATAFQRITNKVFANPEDCAGFGKGENAKKFVRTD 66 +M + L AF +T+++ KM ++ T R+ K FANPED G ++ R+D Sbjct: 9 VMSSPALPAFLLCSTLLVIKMYVVAIITGQVRLRKKAFANPEDALRHG----GPQYCRSD 64 Query: 67 EKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLSTALMHFRIFVGARIYHTIAYLTPLP 126 VER RAH ND+E I PFL +G +YS GP+ A MHF +F+ R+ HT+AYL L Sbjct: 65 PDVERCLRAHRNDMETIYPFLFLGFVYSFLGPNPFVAWMHFLVFLVGRVAHTVAYLGKLR 124 Query: 127 QPNRGLAFFVGYGVTLSMAYRLL 149 P R + + + SMA ++L Sbjct: 125 APIRSVTYTLAQLPCASMALQIL 147 >3NAW-A nrPDB Length = 613 Score = 26.1 bits (56), Expect = 5.8, Method: Composition-based stats. Identities = 7/33 (21%), Positives = 15/33 (45%) Query: 89 IGLLYSLSGPDLSTALMHFRIFVGARIYHTIAY 121 + + + P+ ST M F ++ + +T Y Sbjct: 381 LSHMVDMLSPNTSTNWMSFFLYKDGEVQNTFGY 413 >5CO4-B nrPDB Length = 178 Score = 26.1 bits (56), Expect = 6.6, Method: Composition-based stats. Identities = 13/29 (44%), Positives = 14/29 (48%) Query: 124 PLPQPNRGLAFFVGYGVTLSMAYRLLRSR 152 P+P P R L V GV AYR L R Sbjct: 150 PMPGPVRSLNLAVAVGVAAYEAYRQLTGR 178 >5TEY-A nrPDB Length = 598 Score = 26.1 bits (56), Expect = 6.7, Method: Composition-based stats. Identities = 11/37 (29%), Positives = 21/37 (56%) Query: 63 VRTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPD 99 + TD ++E+ HL+DL +P + + ++S PD Sbjct: 90 LATDPELEKKLLHHLSDLALTLPTDAVSICLAISTPD 126 >3ODM-A nrPDB Length = 560 Score = 25.7 bits (55), Expect = 7.4, Method: Composition-based stats. Identities = 12/52 (23%), Positives = 21/52 (40%), Gaps = 5/52 (9%) Query: 50 CAGFGKGENAKKFVRTDEKVERVRRAHLNDLENIVPFLGIGLLYSLSGPDLS 101 A F G +KK + E R+ + D++ + L +GL Y + Sbjct: 466 AARFANGGVSKKIID-----EEARQEYKEDMKYVNEILNLGLDYDFLNENEF 512 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.140 0.391 Lambda K H 0.267 0.0427 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,898,986 Number of Sequences: 36641 Number of extensions: 234354 Number of successful extensions: 672 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 656 Number of HSP's gapped (non-prelim): 15 length of query: 155 length of database: 10,336,785 effective HSP length: 89 effective length of query: 66 effective length of database: 7,075,736 effective search space: 466998576 effective search space used: 466998576 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.2 bits) S2: 55 (25.7 bits)