BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8WVK7 (121 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4AJ5-K nrPDB 245 3e-66 5VHD-D nrPDB 29 0.58 3J7A-F nrPDB 27 2.8 2RBB-A nrPDB 25 5.6 >4AJ5-K nrPDB Length = 123 Score = 245 bits (626), Expect = 3e-66, Method: Compositional matrix adjust. Identities = 121/121 (100%), Positives = 121/121 (100%) Query: 1 MEAEVDKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTL 60 MEAEVDKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTL Sbjct: 3 MEAEVDKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTL 62 Query: 61 YARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEEKTAAEQFKFHMPD 120 YARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEEKTAAEQFKFHMPD Sbjct: 63 YARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEEKTAAEQFKFHMPD 122 Query: 121 L 121 L Sbjct: 123 L 123 >5VHD-D nrPDB Length = 870 Score = 28.9 bits (63), Expect = 0.58, Method: Compositional matrix adjust. Identities = 13/37 (35%), Positives = 22/37 (59%) Query: 66 PVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSP 102 P V+ K++KSR CA + +++I+ +K T L P Sbjct: 817 PHPVDWKDTKSRDCAVLSAIIDLIKTQEKATPRNLPP 853 >3J7A-F nrPDB Length = 261 Score = 26.6 bits (57), Expect = 2.8, Method: Compositional matrix adjust. Identities = 17/49 (34%), Positives = 27/49 (55%), Gaps = 6/49 (12%) Query: 43 PVTLLKELSVIKSR--YQTLY---ARFKPVAVEQKESKSRICATVKKTM 86 PV L+ + + KS ++ LY RF P + +ESK ++C VKK + Sbjct: 83 PVGLMDVIHITKSNEYFRLLYDIKGRFVPHRITNEESKYKLCK-VKKIL 130 >2RBB-A nrPDB Length = 141 Score = 25.4 bits (54), Expect = 5.6, Method: Compositional matrix adjust. Identities = 17/70 (24%), Positives = 37/70 (52%), Gaps = 2/70 (2%) Query: 9 ELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTLYARFKPVA 68 ELM S+ I++ L +++ T ++ + PV + ++IK+ Y+T Y ++ V Sbjct: 62 ELMQLAQFSETSGIKFLLNFDVDTK--EAVDKLVPVAIAAGATLIKAPYETYYHWYQAVL 119 Query: 69 VEQKESKSRI 78 ++ + + RI Sbjct: 120 LDPERNVFRI 129 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4AJ5-K nrPDB 210 2e-55 Sequences not found previously or not previously below threshold: 5VHD-D nrPDB 31 0.19 2RBB-A nrPDB 28 0.88 5B46-A nrPDB 25 5.6 1L1L-A nrPDB 25 7.3 CONVERGED! >4AJ5-K nrPDB Length = 123 Score = 210 bits (533), Expect = 2e-55, Method: Composition-based stats. Identities = 121/121 (100%), Positives = 121/121 (100%) Query: 1 MEAEVDKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTL 60 MEAEVDKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTL Sbjct: 3 MEAEVDKLELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTL 62 Query: 61 YARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEEKTAAEQFKFHMPD 120 YARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEEKTAAEQFKFHMPD Sbjct: 63 YARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEEKTAAEQFKFHMPD 122 Query: 121 L 121 L Sbjct: 123 L 123 >5VHD-D nrPDB Length = 870 Score = 30.5 bits (67), Expect = 0.19, Method: Composition-based stats. Identities = 13/37 (35%), Positives = 22/37 (59%) Query: 66 PVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSP 102 P V+ K++KSR CA + +++I+ +K T L P Sbjct: 817 PHPVDWKDTKSRDCAVLSAIIDLIKTQEKATPRNLPP 853 >2RBB-A nrPDB Length = 141 Score = 28.2 bits (61), Expect = 0.88, Method: Composition-based stats. Identities = 17/70 (24%), Positives = 37/70 (52%), Gaps = 2/70 (2%) Query: 9 ELMFQKAESDLDYIQYRLEYEIKTNHPDSASEKNPVTLLKELSVIKSRYQTLYARFKPVA 68 ELM S+ I++ L +++ T ++ + PV + ++IK+ Y+T Y ++ V Sbjct: 62 ELMQLAQFSETSGIKFLLNFDVDTK--EAVDKLVPVAIAAGATLIKAPYETYYHWYQAVL 119 Query: 69 VEQKESKSRI 78 ++ + + RI Sbjct: 120 LDPERNVFRI 129 >5B46-A nrPDB Length = 628 Score = 25.5 bits (54), Expect = 5.6, Method: Composition-based stats. Identities = 22/91 (24%), Positives = 39/91 (42%), Gaps = 8/91 (8%) Query: 9 ELMFQKAESDLDYIQYRLEYE------IKTNHPDSASEKNPVTLLKELS-VIKSRYQTLY 61 E +FQ +Y+ Y +EYE +K+ P+ A + + L IK + L Sbjct: 76 ETVFQHFTETKEYLIYNVEYENTTVDLVKSMEPEMAEQVKEALSKERLGFTIKDVLEYLK 135 Query: 62 AR-FKPVAVEQKESKSRICATVKKTMNMIQK 91 R K + E +I T K M+++++ Sbjct: 136 RRGVKVIGFNYTELIKKIADTFKVPMSVVER 166 >1L1L-A nrPDB Length = 739 Score = 25.1 bits (53), Expect = 7.3, Method: Composition-based stats. Identities = 14/51 (27%), Positives = 22/51 (43%) Query: 57 YQTLYARFKPVAVEQKESKSRICATVKKTMNMIQKLQKQTDLELSPLTKEE 107 Y+ YAR+ P + + V+ +N+ +LQ LEL EE Sbjct: 31 YKRTYARWLPEKGRSENWDETVKRVVEGNINLDPRLQDSPSLELKQSLTEE 81 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.126 0.334 Lambda K H 0.267 0.0396 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,871,479 Number of Sequences: 36641 Number of extensions: 116753 Number of successful extensions: 245 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 228 Number of HSP's gapped (non-prelim): 29 length of query: 121 length of database: 10,336,785 effective HSP length: 85 effective length of query: 36 effective length of database: 7,222,300 effective search space: 260002800 effective search space used: 260002800 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 52 (24.7 bits)