BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8WV92 (249 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2YMB-A nrPDB 519 e-148 5XMK-A nrPDB 50 1e-06 1T8Q-A nrPDB 31 0.47 2ZAO-A nrPDB 30 0.66 4LIK-A nrPDB 29 2.2 4LCB-A nrPDB 29 2.4 3IG3-A nrPDB 28 2.7 5WLC-SG nrPDB 28 4.5 4J0X-A nrPDB 28 4.8 >2YMB-A nrPDB Length = 257 Score = 519 bits (1337), Expect = e-148, Method: Compositional matrix adjust. Identities = 249/249 (100%), Positives = 249/249 (100%) Query: 1 MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCN 60 MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCN Sbjct: 9 MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCN 68 Query: 61 LREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWI 120 LREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWI Sbjct: 69 LREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWI 128 Query: 121 EDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSH 180 EDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSH Sbjct: 129 EDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSH 188 Query: 181 GVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDI 240 GVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDI Sbjct: 189 GVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDI 248 Query: 241 FHKKHTKNI 249 FHKKHTKNI Sbjct: 249 FHKKHTKNI 257 >5XMK-A nrPDB Length = 437 Score = 49.7 bits (117), Expect = 1e-06, Method: Compositional matrix adjust. Identities = 23/73 (31%), Positives = 47/73 (64%) Query: 13 TAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRA 72 T ++++A++LD+ ++Y +A Y G+D L+ LK K+ + +R K ++Y++RA Sbjct: 8 TKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNPKSKDLIRAKFTEYLNRA 67 Query: 73 ENIKKYLDQEKED 85 E +KK+L+ E+ + Sbjct: 68 EQLKKHLESEEAN 80 >1T8Q-A nrPDB Length = 336 Score = 31.2 bits (69), Expect = 0.47, Method: Compositional matrix adjust. Identities = 20/64 (31%), Positives = 33/64 (51%), Gaps = 5/64 (7%) Query: 27 SESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAENIK-KYLDQEKED 85 E P + Y +G D L Q L TKD+ NL Y+DR ++ ++ D+ ++D Sbjct: 22 PEHTLPAKAMAYAQGADYLEQDLVMTKDD----NLVVLHDHYLDRVTDVADRFPDRARKD 77 Query: 86 GKYH 89 G+Y+ Sbjct: 78 GRYY 81 >2ZAO-A nrPDB Length = 444 Score = 30.4 bits (67), Expect = 0.66, Method: Compositional matrix adjust. Identities = 18/60 (30%), Positives = 32/60 (53%), Gaps = 1/60 (1%) Query: 15 AATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLK-GTKDNTKRCNLREKISKYMDRAE 73 A + +A + D Y +AL YQ + L V+K + + + ++R K ++Y+DRAE Sbjct: 11 AIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRAE 70 >4LIK-A nrPDB Length = 392 Score = 28.9 bits (63), Expect = 2.2, Method: Compositional matrix adjust. Identities = 16/44 (36%), Positives = 28/44 (63%), Gaps = 1/44 (2%) Query: 39 QEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAEN-IKKYLDQ 81 + GI L ++KG +D K+ +L EKI ++ ++ N IKKY ++ Sbjct: 186 RSGIVEYLSLVKGGQDVKKKVHLSEKIHPFIRKSINIIKKYFEE 229 >4LCB-A nrPDB Length = 367 Score = 28.9 bits (63), Expect = 2.4, Method: Compositional matrix adjust. Identities = 17/69 (24%), Positives = 32/69 (46%) Query: 22 AVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAENIKKYLDQ 81 AV D E +A+ Y++ ID+L Q++ D+ R + I +Y R ++ L + Sbjct: 15 AVRADKEGNRDEAITYYKKAIDVLTQIVVLYPDSPTRQAYEQMIDEYKKRVSVLENMLPE 74 Query: 82 EKEDGKYHK 90 ++ K Sbjct: 75 TPQEDNSQK 83 >3IG3-A nrPDB Length = 627 Score = 28.5 bits (62), Expect = 2.7, Method: Compositional matrix adjust. Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Query: 109 EYLNETVTEVWIEDPYIRHTHQLYNF-LRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQS 167 ++L+E + I DP +RHT + LRF +IK P V IH + D + V Q+ Sbjct: 451 DFLDEQADQRQISDPDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKNSITDACLSVVAQT 510 >5WLC-SG nrPDB Length = 573 Score = 27.7 bits (60), Expect = 4.5, Method: Compositional matrix adjust. Identities = 25/97 (25%), Positives = 40/97 (41%), Gaps = 4/97 (4%) Query: 104 ESLFREYLNETVTEVWIEDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQ 163 + L R ++ E E ED +++ + L FCE I V H +T D G Sbjct: 366 QKLLRRWMKENAKEG--EDGEVKYPDESEAPLFFCEGSIDVVSMVDDFHFITGSDNGNIC 423 Query: 164 VQQSRGLQEIEESLRSHGVLLEVQYS--SSIHDREIR 198 + + I +HG+L E ++ S D E+R Sbjct: 424 LWSLAKKKPIFTERIAHGILPEPSFNDISGETDEELR 460 >4J0X-A nrPDB Length = 451 Score = 27.7 bits (60), Expect = 4.8, Method: Compositional matrix adjust. Identities = 25/97 (25%), Positives = 40/97 (41%), Gaps = 4/97 (4%) Query: 104 ESLFREYLNETVTEVWIEDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQ 163 + L R ++ E E ED +++ + L FCE I V H +T D G Sbjct: 244 QKLLRRWMKENAKEG--EDGEVKYPDESEAPLFFCEGSIDVVSMVDDFHFITGSDNGNIC 301 Query: 164 VQQSRGLQEIEESLRSHGVLLEVQYS--SSIHDREIR 198 + + I +HG+L E ++ S D E+R Sbjct: 302 LWSLAKKKPIFTERIAHGILPEPSFNDISGETDEELR 338 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2YMB-A nrPDB 485 e-138 5XMK-A nrPDB 125 2e-29 Sequences not found previously or not previously below threshold: 2ZAO-A nrPDB 52 2e-07 1T8Q-A nrPDB 32 0.30 2C4R-L nrPDB 30 1.3 1YR7-A nrPDB 29 1.6 4LUS-A nrPDB 29 1.9 3IG3-A nrPDB 29 2.0 5MQ0-T nrPDB 28 3.4 1R19-A nrPDB 28 5.2 5HY3-A nrPDB 28 5.3 4B17-A nrPDB 27 8.1 >2YMB-A nrPDB Length = 257 Score = 485 bits (1248), Expect = e-138, Method: Composition-based stats. Identities = 249/249 (100%), Positives = 249/249 (100%) Query: 1 MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCN 60 MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCN Sbjct: 9 MAKSGLRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCN 68 Query: 61 LREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWI 120 LREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWI Sbjct: 69 LREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWI 128 Query: 121 EDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSH 180 EDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSH Sbjct: 129 EDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSH 188 Query: 181 GVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDI 240 GVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDI Sbjct: 189 GVLLEVQYSSSIHDREIRFNNGWMIKIGRGLDYFKKPQSRFSLGYCDFDLRPCHETTVDI 248 Query: 241 FHKKHTKNI 249 FHKKHTKNI Sbjct: 249 FHKKHTKNI 257 >5XMK-A nrPDB Length = 437 Score = 125 bits (314), Expect = 2e-29, Method: Composition-based stats. Identities = 23/74 (31%), Positives = 47/74 (63%) Query: 13 TAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRA 72 T ++++A++LD+ ++Y +A Y G+D L+ LK K+ + +R K ++Y++RA Sbjct: 8 TKGIELVQKAIDLDTATQYEEAYTAYYNGLDYLMLALKYEKNPKSKDLIRAKFTEYLNRA 67 Query: 73 ENIKKYLDQEKEDG 86 E +KK+L+ E+ + Sbjct: 68 EQLKKHLESEEANA 81 >2ZAO-A nrPDB Length = 444 Score = 52.3 bits (124), Expect = 2e-07, Method: Composition-based stats. Identities = 17/60 (28%), Positives = 31/60 (51%), Gaps = 1/60 (1%) Query: 14 AAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGT-KDNTKRCNLREKISKYMDRA 72 A + +A + D Y +AL YQ + L V+K + + + ++R K ++Y+DRA Sbjct: 10 KAIDLASKAAQEDKAGNYEEALQLYQHAVQYFLHVVKYEAQGDKAKQSIRAKCTEYLDRA 69 >1T8Q-A nrPDB Length = 336 Score = 31.5 bits (70), Expect = 0.30, Method: Composition-based stats. Identities = 24/81 (29%), Positives = 39/81 (48%), Gaps = 11/81 (13%) Query: 32 PQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAENI-KKYLDQEKEDGKYH- 89 P + Y +G D L Q L TKD NL Y+DR ++ ++ D+ ++DG+Y+ Sbjct: 27 PAKAMAYAQGADYLEQDLVMTKD----DNLVVLHDHYLDRVTDVADRFPDRARKDGRYYA 82 Query: 90 -----KQIKIEENATGFSYES 105 +IK + GF E+ Sbjct: 83 IDFTLDEIKSLKFTEGFDIEN 103 >2C4R-L nrPDB Length = 517 Score = 29.6 bits (65), Expect = 1.3, Method: Composition-based stats. Identities = 24/109 (22%), Positives = 47/109 (43%), Gaps = 13/109 (11%) Query: 19 LKRAVELDSESRYPQALVCYQ--EGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAENIK 76 + R +E D + +AL + EG+ L+++ K L+ +S + E IK Sbjct: 146 ISRRIEGDDRTELKEALASLELPEGMGLIVRTAGVGK---SAEALQWDLSFRLKHWEAIK 202 Query: 77 KYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWIEDPYI 125 K + H++ + A FR+YL + + E+ I++P + Sbjct: 203 KAAESRPAPFLIHQESNVIVRA--------FRDYLRQDIGEILIDNPKV 243 >1YR7-A nrPDB Length = 262 Score = 29.2 bits (64), Expect = 1.6, Method: Composition-based stats. Identities = 30/109 (27%), Positives = 43/109 (39%), Gaps = 18/109 (16%) Query: 56 TKRCNLREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYESLFREY---LN 112 L EK ++Y++ K L EKE+ + + G LF E+ L Sbjct: 86 ESYDRLMEKFNEYLN------KILRLEKENDY------VLIDTPGQMETFLFHEFGVRLM 133 Query: 113 ETVTE---VWIEDPYIRHTHQLYNFLRFCEMLIKRPCKVKTIHLLTSLD 158 E + V+I DP I Y F+RF +LI TI L +D Sbjct: 134 ENLPYPLVVYISDPEILKKPNDYCFVRFFALLIDLRLGATTIPALNKVD 182 >4LUS-A nrPDB Length = 385 Score = 28.8 bits (63), Expect = 1.9, Method: Composition-based stats. Identities = 20/88 (22%), Positives = 38/88 (43%), Gaps = 1/88 (1%) Query: 18 VLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKISKYMDRAENIKK 77 + K V+ + +R + + Q GI L + L T D +++ KI+ + E +K Sbjct: 54 LEKEKVDYLAVARTAEGIELRQNGITLPILNLGYTPDEAFEDSIKNKITMTVYSLETAQK 113 Query: 78 YLDQEKEDG-KYHKQIKIEENATGFSYE 104 + K G K +KI+ T ++ Sbjct: 114 INEIAKSLGEKACVHVKIDSGMTRIGFQ 141 >3IG3-A nrPDB Length = 627 Score = 28.8 bits (63), Expect = 2.0, Method: Composition-based stats. Identities = 20/60 (33%), Positives = 30/60 (50%), Gaps = 1/60 (1%) Query: 109 EYLNETVTEVWIEDPYIRHTHQLYNF-LRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQS 167 ++L+E + I DP +RHT + LRF +IK P V IH + D + V Q+ Sbjct: 451 DFLDEQADQRQISDPDVRHTWKSNCLPLRFWVNVIKNPQFVFDIHKNSITDACLSVVAQT 510 >5MQ0-T nrPDB Length = 877 Score = 28.1 bits (61), Expect = 3.4, Method: Composition-based stats. Identities = 32/132 (24%), Positives = 56/132 (42%), Gaps = 26/132 (19%) Query: 76 KKYLDQEKEDGKYHKQIKIEENATGFSYESLFREYLNETVTEVWIEDPYIR--------H 127 K+Y++ KE+G+ KQI+ L+ + ++ VT+ I + YIR Sbjct: 41 KRYIEYWKEEGRTDKQIRW-----------LYERFCSQFVTDTSIWEDYIRWESTKEVVE 89 Query: 128 THQLYNFLRFCEMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSHGVLLEVQ 187 T +++ + C R C L+ L+ IEQ L I +L S + +E + Sbjct: 90 TSRIFWLFQRCLKSCVRDCDRIC---LSYLELAIEQYD----LAMIRHALASSLMKMERE 142 Query: 188 YSSSIHDREIRF 199 + D I+F Sbjct: 143 MHRKVWDPVIKF 154 >1R19-A nrPDB Length = 343 Score = 27.7 bits (60), Expect = 5.2, Method: Composition-based stats. Identities = 20/67 (29%), Positives = 31/67 (46%), Gaps = 6/67 (8%) Query: 51 GTKDNTKRCNLREKISKYMDRAENIKKYLDQEKEDGKYHKQIKIEENATGFSYE-SLFRE 109 GT DNT + + + Y+D+ ENIK +L Y + K+ N T E Sbjct: 105 GTYDNTNK-QITYTFTDYVDKYENIKAHLKLTS----YIDKSKVPNNNTKLDVEYKTALS 159 Query: 110 YLNETVT 116 +N+T+T Sbjct: 160 SVNKTIT 166 >5HY3-A nrPDB Length = 346 Score = 27.7 bits (60), Expect = 5.3, Method: Composition-based stats. Identities = 11/82 (13%), Positives = 37/82 (45%), Gaps = 2/82 (2%) Query: 6 LRQDPQSTAAATVLKRAVELDSESRYPQALVCYQEGIDLLLQVLKGTKDNTKRCNLREKI 65 + +D + + +++ + ++++ E + + Q+L + + NL E Sbjct: 210 IHEDIKKQLSCSLIMKKIDVEMEDYSTYCFSALRAIEGFIYQILNDVCNPSSSKNLGEYF 269 Query: 66 SKYMDRAENIKKYLDQEKEDGK 87 ++ ++ + I + + QE +G+ Sbjct: 270 TE--NKPKYIIREIHQETINGE 289 >4B17-A nrPDB Length = 375 Score = 26.9 bits (58), Expect = 8.1, Method: Composition-based stats. Identities = 16/58 (27%), Positives = 27/58 (46%), Gaps = 2/58 (3%) Query: 139 EMLIKRPCKVKTIHLLTSLDEGIEQVQQSRGLQEIEESLRSHGVLLEVQYSSSIHDRE 196 + L+ C+ +L + + E+V S L I+ L HG+ ++Q HDRE Sbjct: 291 QWLVNGWCRETIFNLKLPMKKRYEEV--SHNLAYIQAQLDEHGINAQIQARQLYHDRE 346 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.137 0.389 Lambda K H 0.267 0.0421 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,920,064 Number of Sequences: 36641 Number of extensions: 400439 Number of successful extensions: 1128 Number of sequences better than 10.0: 19 Number of HSP's better than 10.0 without gapping: 11 Number of HSP's successfully gapped in prelim test: 26 Number of HSP's that attempted gapping in prelim test: 1109 Number of HSP's gapped (non-prelim): 41 length of query: 249 length of database: 10,336,785 effective HSP length: 95 effective length of query: 154 effective length of database: 6,855,890 effective search space: 1055807060 effective search space used: 1055807060 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 58 (26.9 bits)