BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8WTW4 (380 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6CES-N nrPDB 788 0.0 5X7M-A nrPDB 30 2.3 5I4E-A nrPDB 29 3.5 5D98-C nrPDB 29 4.0 1KYQ-A nrPDB 28 5.2 2RFO-A nrPDB 28 7.9 >6CES-N nrPDB Length = 380 Score = 788 bits (2034), Expect = 0.0, Method: Compositional matrix adjust. Identities = 380/380 (100%), Positives = 380/380 (100%) Query: 1 MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAM 60 MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAM Sbjct: 1 MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAM 60 Query: 61 EKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVS 120 EKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVS Sbjct: 61 EKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVS 120 Query: 121 MEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKD 180 MEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKD Sbjct: 121 MEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKD 180 Query: 181 KEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQ 240 KEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQ Sbjct: 181 KEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQ 240 Query: 241 YSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGR 300 YSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGR Sbjct: 241 YSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGR 300 Query: 301 HPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGM 360 HPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGM Sbjct: 301 HPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGM 360 Query: 361 SYHELDERLENDPNIIICWK 380 SYHELDERLENDPNIIICWK Sbjct: 361 SYHELDERLENDPNIIICWK 380 >5X7M-A nrPDB Length = 453 Score = 29.6 bits (65), Expect = 2.3, Method: Compositional matrix adjust. Identities = 12/30 (40%), Positives = 19/30 (63%) Query: 164 PDPPVAQEYDVPVFTKDKEDFFNSQWDLTT 193 P P +A+EY P+F D++DF + D+ T Sbjct: 33 PLPDLAEEYGTPLFVVDEDDFRSRCRDMAT 62 >5I4E-A nrPDB Length = 980 Score = 28.9 bits (63), Expect = 3.5, Method: Compositional matrix adjust. Identities = 23/89 (25%), Positives = 38/89 (42%), Gaps = 6/89 (6%) Query: 101 IVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVI 160 I+KKL + TT +S ++ E+ + + L G C +SN+ L ++ Sbjct: 885 ILKKLENWATT---KSVYLGSNETGDSITAVQAKLKNLEAFDGECQSLEGQSNSDLLSIL 941 Query: 161 EQRPDPPVAQEYDVPVFTKDKEDFFNSQW 189 Q + VP T+ K+ FF QW Sbjct: 942 AQLTE---LNYNGVPELTERKDTFFAQQW 967 >5D98-C nrPDB Length = 782 Score = 28.9 bits (63), Expect = 4.0, Method: Compositional matrix adjust. Identities = 29/99 (29%), Positives = 46/99 (46%), Gaps = 8/99 (8%) Query: 80 ALLFNLGFVCDAQAKTCALEPI--VKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLE 137 ALL++ G V K + I K+ L + ++S + M ESK+K+ ++ LE Sbjct: 462 ALLYSRGIVETNPGKMGSSMGIDGSKRAIKSLRAVTIQSGKIDMPESKEKIHLELSDNLE 521 Query: 138 ELNASGRCT-----LPIDESNTIHLKVIEQRPDPPVAQE 171 ++SGR LP D+ T V Q PD V ++ Sbjct: 522 AFDSSGRIVATILDLPSDKKVTFQ-DVSFQHPDLAVLRD 559 >1KYQ-A nrPDB Length = 274 Score = 28.5 bits (62), Expect = 5.2, Method: Compositional matrix adjust. Identities = 12/31 (38%), Positives = 16/31 (51%) Query: 170 QEYDVPVFTKDKEDFFNSQWDLTTQQILPYI 200 Q D P + +D + F N WD T +I YI Sbjct: 59 QNKDQPDYREDAKRFINPNWDPTKNEIYEYI 89 >2RFO-A nrPDB Length = 651 Score = 27.7 bits (60), Expect = 7.9, Method: Compositional matrix adjust. Identities = 26/96 (27%), Positives = 45/96 (46%), Gaps = 10/96 (10%) Query: 181 KEDFFNSQWDLTTQQ-----ILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYG---V 232 +E +FN QW T Q +LP+ D +K ++++ N+V+ I NLL + Sbjct: 536 RELYFNKQWQETLSQMELLDLLPFSDELSARKKAQDFSNLDDNIVK-NIPNLLIITLSCI 594 Query: 233 VTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSYV 268 ++ IL S Y + K Q + K++ C+ Y Sbjct: 595 SNMIHILNESK-YQSSTKGQQIDSLKNVARQCMIYA 629 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6CES-N nrPDB 812 0.0 Sequences not found previously or not previously below threshold: 5X7M-A nrPDB 30 1.5 2RFO-A nrPDB 30 1.5 1PYP-A nrPDB 30 2.4 1KYQ-A nrPDB 30 2.5 4EJS-B nrPDB 29 3.3 4A8J-B nrPDB 29 3.3 5I4E-A nrPDB 29 3.5 5D98-C nrPDB 28 6.5 CONVERGED! >6CES-N nrPDB Length = 380 Score = 812 bits (2098), Expect = 0.0, Method: Composition-based stats. Identities = 380/380 (100%), Positives = 380/380 (100%) Query: 1 MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAM 60 MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAM Sbjct: 1 MGSGCRIECIFFSEFHPTLGPKITYQVPEDFISRELFDTVQVYIITKPELQNKLITVTAM 60 Query: 61 EKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVS 120 EKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVS Sbjct: 61 EKKLIGCPVCIEHKKYSRNALLFNLGFVCDAQAKTCALEPIVKKLAGYLTTLELESSFVS 120 Query: 121 MEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKD 180 MEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKD Sbjct: 121 MEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKD 180 Query: 181 KEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQ 240 KEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQ Sbjct: 181 KEDFFNSQWDLTTQQILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYGVVTLVSILQ 240 Query: 241 YSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGR 300 YSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGR Sbjct: 241 YSNVYCPTPKVQDLVDDKSLQEACLSYVTKQGHKRASLRDVFQLYCSLSPGTTVRDLIGR 300 Query: 301 HPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGM 360 HPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGM Sbjct: 301 HPQQLQHVDERKLIQFGLMKNLIRRLQKYPVRVTREEQSHPARLYTGCHSYDEICCKTGM 360 Query: 361 SYHELDERLENDPNIIICWK 380 SYHELDERLENDPNIIICWK Sbjct: 361 SYHELDERLENDPNIIICWK 380 >5X7M-A nrPDB Length = 453 Score = 30.4 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 12/30 (40%), Positives = 19/30 (63%) Query: 164 PDPPVAQEYDVPVFTKDKEDFFNSQWDLTT 193 P P +A+EY P+F D++DF + D+ T Sbjct: 33 PLPDLAEEYGTPLFVVDEDDFRSRCRDMAT 62 >2RFO-A nrPDB Length = 651 Score = 30.4 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 26/95 (27%), Positives = 45/95 (47%), Gaps = 10/95 (10%) Query: 181 KEDFFNSQWDLTTQQ-----ILPYIDGFRHIQKISAEADVELNLVRIAIQNLLYYG---V 232 +E +FN QW T Q +LP+ D +K ++++ N+V+ I NLL + Sbjct: 536 RELYFNKQWQETLSQMELLDLLPFSDELSARKKAQDFSNLDDNIVK-NIPNLLIITLSCI 594 Query: 233 VTLVSILQYSNVYCPTPKVQDLVDDKSLQEACLSY 267 ++ IL S Y + K Q + K++ C+ Y Sbjct: 595 SNMIHILNESK-YQSSTKGQQIDSLKNVARQCMIY 628 >1PYP-A nrPDB Length = 285 Score = 29.6 bits (65), Expect = 2.4, Method: Composition-based stats. Identities = 12/41 (29%), Positives = 23/41 (56%) Query: 146 TLPIDESNTIHLKVIEQRPDPPVAQEYDVPVFTKDKEDFFN 186 T I NT+ KV ++ PV+ +D+P++ +++ FN Sbjct: 4 TRQIGAKNTLEYKVYIEKDGKPVSAFHDIPLYADKEDNIFN 44 >1KYQ-A nrPDB Length = 274 Score = 29.6 bits (65), Expect = 2.5, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 16/31 (51%) Query: 170 QEYDVPVFTKDKEDFFNSQWDLTTQQILPYI 200 Q D P + +D + F N WD T +I YI Sbjct: 59 QNKDQPDYREDAKRFINPNWDPTKNEIYEYI 89 >4EJS-B nrPDB Length = 242 Score = 29.2 bits (64), Expect = 3.3, Method: Composition-based stats. Identities = 16/53 (30%), Positives = 26/53 (49%) Query: 110 TTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQ 162 T+ +L FV +SK PI+ I E +N CT ID + + +++Q Sbjct: 38 TSYKLIQEFVHQSKSKGNEYPIVYISFETVNKPSYCTQFIDATQMDFVHLVKQ 90 >4A8J-B nrPDB Length = 270 Score = 29.2 bits (64), Expect = 3.3, Method: Composition-based stats. Identities = 16/53 (30%), Positives = 26/53 (49%) Query: 110 TTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVIEQ 162 T+ +L FV +SK PI+ I E +N CT ID + + +++Q Sbjct: 34 TSYKLIQEFVHQSKSKGNEYPIVYISFETVNKPSYCTQFIDATQMDFVHLVKQ 86 >5I4E-A nrPDB Length = 980 Score = 28.8 bits (63), Expect = 3.5, Method: Composition-based stats. Identities = 24/90 (26%), Positives = 40/90 (44%), Gaps = 8/90 (8%) Query: 101 IVKKLAGYLTTLELESSFVSMEESKQKLVPIMTILLEELNASGRCTLPIDESNTIHLKVI 160 I+KKL + TT +S ++ E+ + + L G C +SN+ L ++ Sbjct: 885 ILKKLENWATT---KSVYLGSNETGDSITAVQAKLKNLEAFDGECQSLEGQSNSDLLSIL 941 Query: 161 EQRPDPPVAQEYD-VPVFTKDKEDFFNSQW 189 Q + Y+ VP T+ K+ FF QW Sbjct: 942 AQLTE----LNYNGVPELTERKDTFFAQQW 967 >5D98-C nrPDB Length = 782 Score = 28.1 bits (61), Expect = 6.5, Method: Composition-based stats. Identities = 30/108 (27%), Positives = 50/108 (46%), Gaps = 8/108 (7%) Query: 71 IEHKKYSRNALLFNLGFVCDAQAKTCALEPI--VKKLAGYLTTLELESSFVSMEESKQKL 128 I+ + + ALL++ G V K + I K+ L + ++S + M ESK+K+ Sbjct: 453 IQKSEDNLEALLYSRGIVETNPGKMGSSMGIDGSKRAIKSLRAVTIQSGKIDMPESKEKI 512 Query: 129 VPIMTILLEELNASGRCT-----LPIDESNTIHLKVIEQRPDPPVAQE 171 ++ LE ++SGR LP D+ T V Q PD V ++ Sbjct: 513 HLELSDNLEAFDSSGRIVATILDLPSDKKVTFQ-DVSFQHPDLAVLRD 559 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.138 0.414 Lambda K H 0.267 0.0433 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 16,494,896 Number of Sequences: 36641 Number of extensions: 711325 Number of successful extensions: 1519 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 11 Number of HSP's that attempted gapping in prelim test: 1507 Number of HSP's gapped (non-prelim): 23 length of query: 380 length of database: 10,336,785 effective HSP length: 99 effective length of query: 281 effective length of database: 6,709,326 effective search space: 1885320606 effective search space used: 1885320606 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 60 (27.6 bits)