BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8W453 (102 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2RKN-A nrPDB 162 4e-41 1SIY-A nrPDB 30 0.17 2ABQ-A nrPDB 28 1.0 5HXB-X nrPDB 27 2.6 5WZS-A nrPDB 26 3.5 4YHJ-A nrPDB 26 3.9 1EP3-A nrPDB 26 5.1 2B5S-A nrPDB 25 5.7 4D61-i nrPDB 25 6.5 >2RKN-A nrPDB Length = 77 Score = 162 bits (409), Expect = 4e-41, Method: Compositional matrix adjust. Identities = 77/77 (100%), Positives = 77/77 (100%) Query: 26 AIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSFGVDPE 85 AIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSFGVDPE Sbjct: 1 AIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSFGVDPE 60 Query: 86 LASALPKQCGLANAPTC 102 LASALPKQCGLANAPTC Sbjct: 61 LASALPKQCGLANAPTC 77 >1SIY-A nrPDB Length = 91 Score = 30.4 bits (67), Expect = 0.17, Method: Compositional matrix adjust. Identities = 22/75 (29%), Positives = 33/75 (44%), Gaps = 10/75 (13%) Query: 30 CGMSQDELNECKPAVSKENPTSPSQPCCTALQH--------ADFACLCGYKNSPWLGSFG 81 CG Q L +C + K PS CCT +++ AD +C + G Sbjct: 3 CGQVQGNLAQCIGFLQKGGVVPPS--CCTGVKNILNSSRTTADRRAVCSCLKAAAGAVRG 60 Query: 82 VDPELASALPKQCGL 96 ++P A ALP +CG+ Sbjct: 61 INPNNAEALPGKCGV 75 >2ABQ-A nrPDB Length = 306 Score = 28.1 bits (61), Expect = 1.0, Method: Compositional matrix adjust. Identities = 14/49 (28%), Positives = 23/49 (46%), Gaps = 1/49 (2%) Query: 22 DTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCG 70 DT + + + G + ELN P + KE+ + + T L+ D L G Sbjct: 86 DTRINVKIKGKQETELNGTAPLIKKEHVQALLEQ-LTELEKGDVLVLAG 133 >5HXB-X nrPDB Length = 199 Score = 26.6 bits (57), Expect = 2.6, Method: Compositional matrix adjust. Identities = 17/65 (26%), Positives = 29/65 (44%), Gaps = 5/65 (7%) Query: 21 VDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSF 80 V+ + I L E ++ +P K++ Q C L+ A CL +K+ P +G F Sbjct: 126 VEITALICLVDKKSGEKSKTRPRFVKQD-----QVCIARLRTAGTICLETFKDFPQMGRF 180 Query: 81 GVDPE 85 + E Sbjct: 181 TLRDE 185 >5WZS-A nrPDB Length = 123 Score = 26.2 bits (56), Expect = 3.5, Method: Compositional matrix adjust. Identities = 9/19 (47%), Positives = 12/19 (63%) Query: 58 TALQHADFACLCGYKNSPW 76 +ALQ+ D+ C CG S W Sbjct: 16 SALQYNDYGCYCGIGGSHW 34 >4YHJ-A nrPDB Length = 584 Score = 25.8 bits (55), Expect = 3.9, Method: Composition-based stats. Identities = 12/47 (25%), Positives = 23/47 (48%) Query: 16 IMAMLVDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQH 62 I+ + +A L + D + EC+ + +ENP+ + CT + H Sbjct: 105 ILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAH 151 >1EP3-A nrPDB Length = 311 Score = 25.8 bits (55), Expect = 5.1, Method: Compositional matrix adjust. Identities = 10/16 (62%), Positives = 13/16 (81%) Query: 79 SFGVDPELASALPKQC 94 +FG DPE+A+AL K C Sbjct: 144 AFGTDPEVAAALVKAC 159 >2B5S-A nrPDB Length = 92 Score = 25.4 bits (54), Expect = 5.7, Method: Compositional matrix adjust. Identities = 22/78 (28%), Positives = 34/78 (43%), Gaps = 14/78 (17%) Query: 30 CGMSQDELNECKPAVSKENPTSPSQPCCTALQHAD----------FACLCGYKNSPWLGS 79 CG L C P V P+ CC +++ + AC C + S + Sbjct: 4 CGQVSSSLAPCIPYVRGGGAVPPA--CCNGIRNVNNLARTTPDRQAACNCLKQLSASV-- 59 Query: 80 FGVDPELASALPKQCGLA 97 GV+P A+ALP +CG++ Sbjct: 60 PGVNPNNAAALPGKCGVS 77 >4D61-i nrPDB Length = 426 Score = 25.4 bits (54), Expect = 6.5, Method: Composition-based stats. Identities = 17/65 (26%), Positives = 29/65 (44%), Gaps = 5/65 (7%) Query: 21 VDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSF 80 V+ + I L E ++ +P K++ Q C L+ A CL +K+ P +G F Sbjct: 352 VEITALICLVDKKSGEKSKTRPRFVKQD-----QVCIARLRTAGTICLETFKDFPQMGRF 406 Query: 81 GVDPE 85 + E Sbjct: 407 TLRDE 411 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2RKN-A nrPDB 147 1e-36 Sequences not found previously or not previously below threshold: 1SIY-A nrPDB 30 0.20 1EP3-A nrPDB 27 1.6 5WZS-A nrPDB 26 3.3 4YHJ-A nrPDB 26 3.7 5UE9-A nrPDB 26 4.2 CONVERGED! >2RKN-A nrPDB Length = 77 Score = 147 bits (371), Expect = 1e-36, Method: Composition-based stats. Identities = 77/77 (100%), Positives = 77/77 (100%) Query: 26 AIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSFGVDPE 85 AIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSFGVDPE Sbjct: 1 AIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQHADFACLCGYKNSPWLGSFGVDPE 60 Query: 86 LASALPKQCGLANAPTC 102 LASALPKQCGLANAPTC Sbjct: 61 LASALPKQCGLANAPTC 77 >1SIY-A nrPDB Length = 91 Score = 30.1 bits (66), Expect = 0.20, Method: Composition-based stats. Identities = 22/75 (29%), Positives = 33/75 (44%), Gaps = 10/75 (13%) Query: 30 CGMSQDELNECKPAVSKENPTSPSQPCCTALQH--------ADFACLCGYKNSPWLGSFG 81 CG Q L +C + K PS CCT +++ AD +C + G Sbjct: 3 CGQVQGNLAQCIGFLQKGGVVPPS--CCTGVKNILNSSRTTADRRAVCSCLKAAAGAVRG 60 Query: 82 VDPELASALPKQCGL 96 ++P A ALP +CG+ Sbjct: 61 INPNNAEALPGKCGV 75 >1EP3-A nrPDB Length = 311 Score = 27.4 bits (59), Expect = 1.6, Method: Composition-based stats. Identities = 10/16 (62%), Positives = 13/16 (81%) Query: 79 SFGVDPELASALPKQC 94 +FG DPE+A+AL K C Sbjct: 144 AFGTDPEVAAALVKAC 159 >5WZS-A nrPDB Length = 123 Score = 26.2 bits (56), Expect = 3.3, Method: Composition-based stats. Identities = 9/19 (47%), Positives = 12/19 (63%) Query: 58 TALQHADFACLCGYKNSPW 76 +ALQ+ D+ C CG S W Sbjct: 16 SALQYNDYGCYCGIGGSHW 34 >4YHJ-A nrPDB Length = 584 Score = 26.2 bits (56), Expect = 3.7, Method: Composition-based stats. Identities = 12/47 (25%), Positives = 23/47 (48%) Query: 16 IMAMLVDTSVAIDLCGMSQDELNECKPAVSKENPTSPSQPCCTALQH 62 I+ + +A L + D + EC+ + +ENP+ + CT + H Sbjct: 105 ILDRFFNDKLAAPLPEIPPDVVTECRLGLKEENPSKKAFEECTRVAH 151 >5UE9-A nrPDB Length = 306 Score = 25.8 bits (55), Expect = 4.2, Method: Composition-based stats. Identities = 9/16 (56%), Positives = 13/16 (81%) Query: 79 SFGVDPELASALPKQC 94 +FG DP++A+AL K C Sbjct: 140 AFGTDPDVAAALVKAC 155 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.129 0.409 Lambda K H 0.267 0.0405 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,587,815 Number of Sequences: 36641 Number of extensions: 117410 Number of successful extensions: 205 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 191 Number of HSP's gapped (non-prelim): 23 length of query: 102 length of database: 10,336,785 effective HSP length: 69 effective length of query: 33 effective length of database: 7,808,556 effective search space: 257682348 effective search space used: 257682348 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 52 (24.7 bits)