BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8T045 (411 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5F86-A nrPDB 813 0.0 5L0V-A nrPDB 421 e-118 2H5G-A nrPDB 31 1.0 2YF0-A nrPDB 31 1.3 2PX0-A nrPDB 29 4.4 2W45-A nrPDB 28 6.7 >5F86-A nrPDB Length = 402 Score = 813 bits (2101), Expect = 0.0, Method: Compositional matrix adjust. Identities = 388/391 (99%), Positives = 388/391 (99%) Query: 21 EDDGLCSADQKSCAQSEPDQINEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVL 80 EDDGLCSADQKSCAQSEPDQINEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVL Sbjct: 9 EDDGLCSADQKSCAQSEPDQINEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVL 68 Query: 81 KRDLAPYKSTGVTRQMIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATL 140 KRDLAPYKSTGVTRQMIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATL Sbjct: 69 KRDLAPYKSTGVTRQMIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATL 128 Query: 141 PDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGI 200 PDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGI Sbjct: 129 PDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGI 188 Query: 201 GRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQG 260 GRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQG Sbjct: 189 GRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQG 248 Query: 261 WKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFF 320 WKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFF Sbjct: 249 WKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFF 308 Query: 321 YDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYW 380 YDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYW Sbjct: 309 YDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYW 368 Query: 381 RKLLKRYVKLLQYEVKPEDQLIYIGPKKDEL 411 RKLLKRYVKLLQYEVKPEDQLIYIGPK L Sbjct: 369 RKLLKRYVKLLQYEVKPEDQLIYIGPKARAL 399 >5L0V-A nrPDB Length = 357 Score = 421 bits (1082), Expect = e-118, Method: Compositional matrix adjust. Identities = 208/356 (58%), Positives = 255/356 (71%), Gaps = 15/356 (4%) Query: 53 QIEKANADYKPCSSDPQDSDCSCHANVLKRDLAPYKSTGVTRQMIESSAR--YGTKYKIY 110 QI ++ +Y+PCSS +CSC+ V++ DL P++ G++R+M+ R GT Y+I Sbjct: 10 QINRSLENYEPCSS----QNCSCYHGVIEEDLTPFRG-GISRKMMAEVVRRKLGTHYQIT 64 Query: 111 GHRLYRDANCMFPARCEGIEHFLLPLVATLPDMDLIINTRDYPQLNAAWGNAAGGPVFSF 170 +RLYR+ +CMFP+RC G+EHF+L ++ LPDM+++IN RDYPQ+ W A PVFSF Sbjct: 65 KNRLYRENDCMFPSRCSGVEHFILEVIGRLPDMEMVINVRDYPQV-PKWMEPAI-PVFSF 122 Query: 171 SKTKEYRDIMYPAWTFWAGGPAT-KLHPRGIGRWDQMREKLEKRAAAIPWSQKRSLGFFR 229 SKT EY DIMYPAWTFW GGPA ++P G+GRWD RE L + AA PW +K S +FR Sbjct: 123 SKTSEYHDIMYPAWTFWEGGPAVWPIYPTGLGRWDLFREDLVRSAAQWPWKKKNSTAYFR 182 Query: 230 GSRTSDERDSLILLSRRNPELVEAQYTKNQGWKSPKDTLDAPAADEVSFEDHCKYKYLFN 289 GSRTS ERD LILLSR+NP+LV+A+YTKNQ WKS KDTL PAA +V DHCKYKYLFN Sbjct: 183 GSRTSPERDPLILLSRKNPKLVDAEYTKNQAWKSMKDTLGKPAAKDVHLVDHCKYKYLFN 242 Query: 290 FRGVAASFRLKHLFLCKSLVFHVGDEWQEFFYDQLKPWVHYVPLKSYPSQQEYEHILSFF 349 FRGVAASFR KHLFLC SLVFHVGDEW EFFY QLKPWVHY+P+K+ + +L F Sbjct: 243 FRGVAASFRFKHLFLCGSLVFHVGDEWLEFFYPQLKPWVHYIPVKT--DLSNVQELLQFV 300 Query: 350 KKNDALAQEIAQRGYDFIWEHLRMKDIKCYWRKLLKRYVKLLQYEV---KPEDQLI 402 K ND +AQEIA+RG FI HL+M DI CYW LL Y K L Y V K DQ+I Sbjct: 301 KANDDVAQEIAERGSQFIRNHLQMDDITCYWENLLSEYSKFLSYNVTRRKGYDQII 356 >2H5G-A nrPDB Length = 463 Score = 30.8 bits (68), Expect = 1.0, Method: Compositional matrix adjust. Identities = 24/68 (35%), Positives = 32/68 (47%), Gaps = 4/68 (5%) Query: 113 RLYRDANCMFPARCEGIEHFLL--PLVATLPDMDLIINTRDYPQLNAAWG-NAAGGPVFS 169 RL RD+ C +PA C +E L+ L+ T P D II+ Q+ G A FS Sbjct: 267 RLVRDSKCEYPAACNALETLLIHRDLLRT-PLFDQIIDMLRVEQVKIHAGPKFASYLTFS 325 Query: 170 FSKTKEYR 177 S+ K R Sbjct: 326 PSEVKSLR 333 >2YF0-A nrPDB Length = 512 Score = 30.8 bits (68), Expect = 1.3, Method: Compositional matrix adjust. Identities = 33/123 (26%), Positives = 48/123 (39%), Gaps = 26/123 (21%) Query: 53 QIEKANADYKPCSSDPQDSDCSCHANVLKRDLAPYKSTGVTRQMIESSARYGTKYKI--- 109 Q+ AN DYK C + P R+L Y ++ +I S+++ +K + Sbjct: 145 QLSDANRDYKICETYP-------------REL--YVPRIASKPIIVGSSKFRSKGRFPVL 189 Query: 110 -YGHRLYRDANCM-------FPARCEGIEHFLLPLVATLPDMDLIINTRDYPQLNAAWGN 161 Y H+ A C F ARC EH L + P + P+LNA Sbjct: 190 SYYHQDKEAAICRCSQPLSGFSARCLEDEHLLQAISKANPVNRYMYVMDTRPKLNAMANR 249 Query: 162 AAG 164 AAG Sbjct: 250 AAG 252 >2PX0-A nrPDB Length = 296 Score = 28.9 bits (63), Expect = 4.4, Method: Compositional matrix adjust. Identities = 24/102 (23%), Positives = 43/102 (42%), Gaps = 8/102 (7%) Query: 271 PAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLC------KSLVFHVGDEWQEFFYDQL 324 P+AD+ H KY LF G + L L K + F D ++ +QL Sbjct: 94 PSADKWQEPIHSKYIVLFGSTGAGKTTTLAKLAAISMLEKHKKIAFITTDTYRIAAVEQL 153 Query: 325 KPWVHYV--PLKSYPSQQEYEHILSFFKKNDALAQEIAQRGY 364 K + + PL+ +++E++ F + D + + A R + Sbjct: 154 KTYAELLQAPLEVCYTKEEFQQAKELFSEYDHVFVDTAGRNF 195 >2W45-A nrPDB Length = 470 Score = 28.1 bits (61), Expect = 6.7, Method: Compositional matrix adjust. Identities = 19/68 (27%), Positives = 30/68 (44%), Gaps = 10/68 (14%) Query: 278 FEDHCKYKYLFN---FRGVAASFRLKHLFLCKSLVFHVGDEWQEFFYDQLKPWVHYVPLK 334 +E C++KYLF+ F + S+ + CK + F +P V YVP Sbjct: 224 YEIKCRFKYLFSKSEFDPIYPSYTALYKRPCKR-------SFIRFINSIARPTVEYVPDG 276 Query: 335 SYPSQQEY 342 PS+ +Y Sbjct: 277 RLPSEGDY 284 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5F86-A nrPDB 776 0.0 5L0V-A nrPDB 606 e-174 Sequences not found previously or not previously below threshold: 2H5G-A nrPDB 31 1.0 5ZHZ-A nrPDB 30 2.4 5KYM-A nrPDB 30 2.5 4PDK-A nrPDB 28 5.7 5MG3-D nrPDB 28 7.9 1FEP-A nrPDB 28 8.1 CONVERGED! >5F86-A nrPDB Length = 402 Score = 776 bits (2003), Expect = 0.0, Method: Composition-based stats. Identities = 388/391 (99%), Positives = 388/391 (99%) Query: 21 EDDGLCSADQKSCAQSEPDQINEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVL 80 EDDGLCSADQKSCAQSEPDQINEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVL Sbjct: 9 EDDGLCSADQKSCAQSEPDQINEDEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHANVL 68 Query: 81 KRDLAPYKSTGVTRQMIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATL 140 KRDLAPYKSTGVTRQMIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATL Sbjct: 69 KRDLAPYKSTGVTRQMIESSARYGTKYKIYGHRLYRDANCMFPARCEGIEHFLLPLVATL 128 Query: 141 PDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGI 200 PDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGI Sbjct: 129 PDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTFWAGGPATKLHPRGI 188 Query: 201 GRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQG 260 GRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQG Sbjct: 189 GRWDQMREKLEKRAAAIPWSQKRSLGFFRGSRTSDERDSLILLSRRNPELVEAQYTKNQG 248 Query: 261 WKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFF 320 WKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFF Sbjct: 249 WKSPKDTLDAPAADEVSFEDHCKYKYLFNFRGVAASFRLKHLFLCKSLVFHVGDEWQEFF 308 Query: 321 YDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYW 380 YDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYW Sbjct: 309 YDQLKPWVHYVPLKSYPSQQEYEHILSFFKKNDALAQEIAQRGYDFIWEHLRMKDIKCYW 368 Query: 381 RKLLKRYVKLLQYEVKPEDQLIYIGPKKDEL 411 RKLLKRYVKLLQYEVKPEDQLIYIGPK L Sbjct: 369 RKLLKRYVKLLQYEVKPEDQLIYIGPKARAL 399 >5L0V-A nrPDB Length = 357 Score = 606 bits (1562), Expect = e-174, Method: Composition-based stats. Identities = 208/356 (58%), Positives = 255/356 (71%), Gaps = 15/356 (4%) Query: 53 QIEKANADYKPCSSDPQDSDCSCHANVLKRDLAPYKSTGVTRQMIESSAR--YGTKYKIY 110 QI ++ +Y+PCSS +CSC+ V++ DL P++ G++R+M+ R GT Y+I Sbjct: 10 QINRSLENYEPCSS----QNCSCYHGVIEEDLTPFRG-GISRKMMAEVVRRKLGTHYQIT 64 Query: 111 GHRLYRDANCMFPARCEGIEHFLLPLVATLPDMDLIINTRDYPQLNAAWGNAAGGPVFSF 170 +RLYR+ +CMFP+RC G+EHF+L ++ LPDM+++IN RDYPQ+ W A PVFSF Sbjct: 65 KNRLYRENDCMFPSRCSGVEHFILEVIGRLPDMEMVINVRDYPQV-PKWMEPAI-PVFSF 122 Query: 171 SKTKEYRDIMYPAWTFWAGGPAT-KLHPRGIGRWDQMREKLEKRAAAIPWSQKRSLGFFR 229 SKT EY DIMYPAWTFW GGPA ++P G+GRWD RE L + AA PW +K S +FR Sbjct: 123 SKTSEYHDIMYPAWTFWEGGPAVWPIYPTGLGRWDLFREDLVRSAAQWPWKKKNSTAYFR 182 Query: 230 GSRTSDERDSLILLSRRNPELVEAQYTKNQGWKSPKDTLDAPAADEVSFEDHCKYKYLFN 289 GSRTS ERD LILLSR+NP+LV+A+YTKNQ WKS KDTL PAA +V DHCKYKYLFN Sbjct: 183 GSRTSPERDPLILLSRKNPKLVDAEYTKNQAWKSMKDTLGKPAAKDVHLVDHCKYKYLFN 242 Query: 290 FRGVAASFRLKHLFLCKSLVFHVGDEWQEFFYDQLKPWVHYVPLKSYPSQQEYEHILSFF 349 FRGVAASFR KHLFLC SLVFHVGDEW EFFY QLKPWVHY+P+K+ + +L F Sbjct: 243 FRGVAASFRFKHLFLCGSLVFHVGDEWLEFFYPQLKPWVHYIPVKT--DLSNVQELLQFV 300 Query: 350 KKNDALAQEIAQRGYDFIWEHLRMKDIKCYWRKLLKRYVKLLQYEV---KPEDQLI 402 K ND +AQEIA+RG FI HL+M DI CYW LL Y K L Y V K DQ+I Sbjct: 301 KANDDVAQEIAERGSQFIRNHLQMDDITCYWENLLSEYSKFLSYNVTRRKGYDQII 356 >2H5G-A nrPDB Length = 463 Score = 30.7 bits (68), Expect = 1.0, Method: Composition-based stats. Identities = 21/74 (28%), Positives = 32/74 (43%), Gaps = 8/74 (10%) Query: 113 RLYRDANCMFPARCEGIEHFLLPL-VATLPDMDLIINTRDYPQLNAAWGNAAGGPVFSFS 171 RL RD+ C +PA C +E L+ + P D II+ Q+ G +FS Sbjct: 267 RLVRDSKCEYPAACNALETLLIHRDLLRTPLFDQIIDMLRVEQVKIHAG-PKFASYLTFS 325 Query: 172 KTK------EYRDI 179 ++ EY D+ Sbjct: 326 PSEVKSLRTEYGDL 339 >5ZHZ-A nrPDB Length = 258 Score = 29.6 bits (65), Expect = 2.4, Method: Composition-based stats. Identities = 17/61 (27%), Positives = 23/61 (37%), Gaps = 1/61 (1%) Query: 230 GSRTSDERDSLILLSRRNPELVEAQYTKNQGWKSPKDTLDAPAADEVSFEDHCKYKYLFN 289 GS S D L ++V+ Q WK+PK DA A + + YL N Sbjct: 4 GSHVSPT-DPLAAAEAEGADVVQIFLGNPQSWKAPKPRDDAAALKAATLPIYVHAPYLIN 62 Query: 290 F 290 Sbjct: 63 L 63 >5KYM-A nrPDB Length = 247 Score = 29.6 bits (65), Expect = 2.5, Method: Composition-based stats. Identities = 23/97 (23%), Positives = 36/97 (37%), Gaps = 11/97 (11%) Query: 127 EGIEHFLLPLVATLPDMDLIINTRDYPQLNAAWGNAAGGPVFSFSKTKEYRDIMYPAWTF 186 G+ + P PD ++ +D + G PV S Y I WTF Sbjct: 147 NGVTFIVFPEGTRSPDGKVLSFKKDSLMIAVK----TGVPVLPVSIWGTYHLIPKGRWTF 202 Query: 187 WAGGPATKLH----PRGIGRWDQMR---EKLEKRAAA 216 G K+H P+G +++R E++ KR Sbjct: 203 TPGKVFLKIHEPVDPKGFSSEEELRKYVEEVVKRGVE 239 >4PDK-A nrPDB Length = 279 Score = 28.4 bits (62), Expect = 5.7, Method: Composition-based stats. Identities = 21/92 (22%), Positives = 37/92 (40%), Gaps = 11/92 (11%) Query: 293 VAASFRLKHLFLCKSLVFHVGDEWQEFFYDQLKPWVHYVPLKSYPSQQEYEHILSFFKK- 351 +A +F L + L FH G++ ++ LK V + + Q E + F+++ Sbjct: 177 IAKTFNFYDYMLFQRLAFHSGNQIYGLIFNGLKKLYDRVGSYYFSNPQARELAMEFYRQL 236 Query: 352 --------NDALAQEIAQRG--YDFIWEHLRM 373 + L Q I Q G IW ++M Sbjct: 237 LAVCQSGEREHLPQVIRQYGIASGHIWNQMKM 268 >5MG3-D nrPDB Length = 622 Score = 28.0 bits (61), Expect = 7.9, Method: Composition-based stats. Identities = 20/69 (28%), Positives = 33/69 (47%), Gaps = 10/69 (14%) Query: 204 DQMREKLEKRAAAIPW---SQKRSLGF---FRGSRTSDERDSLILLSRRNPELVEAQYTK 257 D +R L R IP+ ++ + G FR ++ DE ++ LS+R+P+LV + Sbjct: 159 DSLRSDL--REKGIPYTTVRKENNYGLSITFRDAKARDE--AIAYLSKRHPDLVISSQGS 214 Query: 258 NQGWKSPKD 266 NQ D Sbjct: 215 NQLRAVMSD 223 >1FEP-A nrPDB Length = 724 Score = 28.0 bits (61), Expect = 8.1, Method: Composition-based stats. Identities = 25/104 (24%), Positives = 39/104 (37%), Gaps = 29/104 (27%) Query: 44 DEFSFKIRRQIEKANADYKPCSSDPQDSDCSCHA----------------NVLKRDLAPY 87 DEFSF++ ++K AD + Q + +A V++ D AP Sbjct: 185 DEFSFRLYGNLDKTQADAWDINQGHQSARAGTYATTLPAGREGVINKDINGVVRWDFAPL 244 Query: 88 KS----TGVTR---------QMIESSARYGTKYKIYGHRLYRDA 118 +S G +R Q S + +KY +RLYR Sbjct: 245 QSLELEAGYSRQGNLYAGDTQNTNSDSYTRSKYGDETNRLYRQN 288 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.140 0.435 Lambda K H 0.267 0.0426 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 18,509,713 Number of Sequences: 36641 Number of extensions: 829698 Number of successful extensions: 1701 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 1683 Number of HSP's gapped (non-prelim): 23 length of query: 411 length of database: 10,336,785 effective HSP length: 99 effective length of query: 312 effective length of database: 6,709,326 effective search space: 2093309712 effective search space used: 2093309712 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 60 (27.7 bits)