BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8N4Q1 (142 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2K3J-A nrPDB 294 8e-81 3A3C-A nrPDB 92 8e-20 4ZJC-A nrPDB 28 1.5 3N6R-B nrPDB 27 3.7 5WS3-A nrPDB 27 4.0 4UEE-A nrPDB 26 4.8 6GMH-I nrPDB 26 5.5 5OM9-A nrPDB 26 5.9 4KNA-A nrPDB 25 9.8 >2K3J-A nrPDB Length = 146 Score = 294 bits (753), Expect = 8e-81, Method: Compositional matrix adjust. Identities = 142/142 (100%), Positives = 142/142 (100%) Query: 1 MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA 60 MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA Sbjct: 5 MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA 64 Query: 61 SGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP 120 SGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP Sbjct: 65 SGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP 124 Query: 121 AEQAEETAPIEATATKEEEGSS 142 AEQAEETAPIEATATKEEEGSS Sbjct: 125 AEQAEETAPIEATATKEEEGSS 146 >3A3C-A nrPDB Length = 451 Score = 92.0 bits (227), Expect = 8e-20, Method: Compositional matrix adjust. Identities = 39/62 (62%), Positives = 49/62 (79%) Query: 47 GNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYP 106 G INW+ P LGGMA GPCGE+FKSAFSCF YS E KG DCV++F+ MQ+C +KYP+ Y Sbjct: 388 GEINWDSPSLGGMAHGPCGEEFKSAFSCFVYSEAEPKGIDCVEKFQHMQDCFRKYPEHYA 447 Query: 107 QE 108 ++ Sbjct: 448 EQ 449 >4ZJC-A nrPDB Length = 553 Score = 28.1 bits (61), Expect = 1.5, Method: Composition-based stats. Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 3/35 (8%) Query: 40 HGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSC 74 H L+ N N P + SG EQFK+AFSC Sbjct: 507 HWLVYANSAAN---PIIYNFLSGKFREQFKAAFSC 538 >3N6R-B nrPDB Length = 531 Score = 26.6 bits (57), Expect = 3.7, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 15/30 (50%) Query: 14 FVTKEDHETPSSAELVADDPNDPYEEHGLI 43 F D PS LV D+PN PY+ LI Sbjct: 279 FFDDPDRIEPSLDTLVPDNPNTPYDMKELI 308 >5WS3-A nrPDB Length = 560 Score = 26.6 bits (57), Expect = 4.0, Method: Composition-based stats. Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 3/35 (8%) Query: 40 HGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSC 74 H L+ N N P + SG E+FK+AFSC Sbjct: 514 HWLVYANSAAN---PIIYNFLSGKFREEFKAAFSC 545 >4UEE-A nrPDB Length = 309 Score = 26.2 bits (56), Expect = 4.8, Method: Compositional matrix adjust. Identities = 29/104 (27%), Positives = 40/104 (38%), Gaps = 15/104 (14%) Query: 48 NINWNCPC-LGGMASGPCGEQFKSAFSCFHYSTEEI----KGSDCVDQFRAMQECMQ--K 100 N NW+ L G +S PC E + F+ + I K + F ++ Q Sbjct: 144 NRNWDAGFGLSGASSNPCSETYHGKFANSEVEVKSIVDFVKDHGNIKAFISIHSYSQLLM 203 Query: 101 YPDLY---PQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGS 141 YP Y P D+DE ++ K A TA TK GS Sbjct: 204 YPYGYKTEPVPDQDELDQLSK-----AAVTALASLYGTKFNYGS 242 >6GMH-I nrPDB Length = 125 Score = 26.2 bits (56), Expect = 5.5, Method: Compositional matrix adjust. Identities = 11/32 (34%), Positives = 17/32 (53%) Query: 5 RQEGKDRIIFVTKEDHETPSSAELVADDPNDP 36 +QE + I+V K HE +++AD DP Sbjct: 45 QQEADNSCIYVNKITHEVDELTQIIADVSQDP 76 >5OM9-A nrPDB Length = 419 Score = 26.2 bits (56), Expect = 5.9, Method: Compositional matrix adjust. Identities = 29/104 (27%), Positives = 40/104 (38%), Gaps = 15/104 (14%) Query: 48 NINWNCPC-LGGMASGPCGEQFKSAFSCFHYSTEEI----KGSDCVDQFRAMQECMQ--K 100 N NW+ L G +S PC E + F+ + I K + F ++ Q Sbjct: 254 NRNWDAGFGLSGASSNPCSETYHGKFANSEVEVKSIVDFVKDHGNIKAFISIHSYSQLLM 313 Query: 101 YPDLY---PQEDEDEEEEREKKPAEQAEETAPIEATATKEEEGS 141 YP Y P D+DE ++ K A TA TK GS Sbjct: 314 YPYGYKTEPVPDQDELDQLSK-----AAVTALASLYGTKFNYGS 352 >4KNA-A nrPDB Length = 495 Score = 25.4 bits (54), Expect = 9.8, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 22/47 (46%), Gaps = 1/47 (2%) Query: 25 SAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQFKSA 71 SA L+ADD + + G +NWN P G ++ P G +S Sbjct: 413 SAGLLADD-EQAWHTFRRAIRAGIVNWNRPTNGASSAAPFGGAGRSG 458 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2K3J-A nrPDB 256 2e-69 3A3C-A nrPDB 139 4e-34 Sequences not found previously or not previously below threshold: 5OOL-q nrPDB 29 1.1 3N6R-B nrPDB 29 1.2 4ZJC-A nrPDB 28 1.6 5WS3-A nrPDB 28 1.7 5OM9-A nrPDB 27 2.2 4UEE-A nrPDB 27 2.2 2WIN-B nrPDB 27 2.3 1JIF-A nrPDB 27 2.6 6GAW-Bv nrPDB 27 2.8 1B9C-A nrPDB 27 3.6 3NMS-C nrPDB 26 5.5 6GMH-I nrPDB 26 5.6 4CE4-u nrPDB 26 6.7 CONVERGED! >2K3J-A nrPDB Length = 146 Score = 256 bits (655), Expect = 2e-69, Method: Composition-based stats. Identities = 142/142 (100%), Positives = 142/142 (100%) Query: 1 MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA 60 MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA Sbjct: 5 MSYCRQEGKDRIIFVTKEDHETPSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMA 64 Query: 61 SGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP 120 SGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP Sbjct: 65 SGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYPQEDEDEEEEREKKP 124 Query: 121 AEQAEETAPIEATATKEEEGSS 142 AEQAEETAPIEATATKEEEGSS Sbjct: 125 AEQAEETAPIEATATKEEEGSS 146 >3A3C-A nrPDB Length = 451 Score = 139 bits (350), Expect = 4e-34, Method: Composition-based stats. Identities = 39/62 (62%), Positives = 49/62 (79%) Query: 47 GNINWNCPCLGGMASGPCGEQFKSAFSCFHYSTEEIKGSDCVDQFRAMQECMQKYPDLYP 106 G INW+ P LGGMA GPCGE+FKSAFSCF YS E KG DCV++F+ MQ+C +KYP+ Y Sbjct: 388 GEINWDSPSLGGMAHGPCGEEFKSAFSCFVYSEAEPKGIDCVEKFQHMQDCFRKYPEHYA 447 Query: 107 QE 108 ++ Sbjct: 448 EQ 449 >5OOL-q nrPDB Length = 222 Score = 28.5 bits (62), Expect = 1.1, Method: Composition-based stats. Identities = 13/48 (27%), Positives = 25/48 (52%), Gaps = 1/48 (2%) Query: 89 DQFRAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATK 136 ++ + + ECM K P + + + E EK A++ E A ++A A + Sbjct: 116 EREQHIAECMAKMPQMIVNWQQQQRENWEKAQADK-ERRARLQAEAQE 162 >3N6R-B nrPDB Length = 531 Score = 28.5 bits (62), Expect = 1.2, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 15/30 (50%) Query: 14 FVTKEDHETPSSAELVADDPNDPYEEHGLI 43 F D PS LV D+PN PY+ LI Sbjct: 279 FFDDPDRIEPSLDTLVPDNPNTPYDMKELI 308 >4ZJC-A nrPDB Length = 553 Score = 27.8 bits (60), Expect = 1.6, Method: Composition-based stats. Identities = 15/35 (42%), Positives = 18/35 (51%), Gaps = 3/35 (8%) Query: 40 HGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSC 74 H L+ N N P + SG EQFK+AFSC Sbjct: 507 HWLVYANSAAN---PIIYNFLSGKFREQFKAAFSC 538 >5WS3-A nrPDB Length = 560 Score = 27.8 bits (60), Expect = 1.7, Method: Composition-based stats. Identities = 14/35 (40%), Positives = 18/35 (51%), Gaps = 3/35 (8%) Query: 40 HGLILPNGNINWNCPCLGGMASGPCGEQFKSAFSC 74 H L+ N N P + SG E+FK+AFSC Sbjct: 514 HWLVYANSAAN---PIIYNFLSGKFREEFKAAFSC 545 >5OM9-A nrPDB Length = 419 Score = 27.4 bits (59), Expect = 2.2, Method: Composition-based stats. Identities = 30/107 (28%), Positives = 39/107 (36%), Gaps = 21/107 (19%) Query: 48 NINWNCP-CLGGMASGPCGEQFKSAFSCFHYSTEEIKGS-DCVDQFR--------AMQEC 97 N NW+ L G +S PC E + F+ S E+K D V Sbjct: 254 NRNWDAGFGLSGASSNPCSETYHGKFA---NSEVEVKSIVDFVKDHGNIKAFISIHSYSQ 310 Query: 98 MQKYPDLYPQE---DEDEEEEREKKPAEQAEETAPIEATATKEEEGS 141 + YP Y E D+DE ++ K A TA TK GS Sbjct: 311 LLMYPYGYKTEPVPDQDELDQLSK-----AAVTALASLYGTKFNYGS 352 >4UEE-A nrPDB Length = 309 Score = 27.4 bits (59), Expect = 2.2, Method: Composition-based stats. Identities = 30/107 (28%), Positives = 39/107 (36%), Gaps = 21/107 (19%) Query: 48 NINWNCP-CLGGMASGPCGEQFKSAFSCFHYSTEEIKGS-DCVDQFR--------AMQEC 97 N NW+ L G +S PC E + F+ S E+K D V Sbjct: 144 NRNWDAGFGLSGASSNPCSETYHGKFA---NSEVEVKSIVDFVKDHGNIKAFISIHSYSQ 200 Query: 98 MQKYPDLYPQE---DEDEEEEREKKPAEQAEETAPIEATATKEEEGS 141 + YP Y E D+DE ++ K A TA TK GS Sbjct: 201 LLMYPYGYKTEPVPDQDELDQLSK-----AAVTALASLYGTKFNYGS 242 >2WIN-B nrPDB Length = 915 Score = 27.4 bits (59), Expect = 2.3, Method: Composition-based stats. Identities = 7/17 (41%), Positives = 14/17 (82%) Query: 103 DLYPQEDEDEEEEREKK 119 + +P+EDE ++EE +K+ Sbjct: 881 EHWPEEDECQDEENQKQ 897 >1JIF-A nrPDB Length = 122 Score = 27.4 bits (59), Expect = 2.6, Method: Composition-based stats. Identities = 15/75 (20%), Positives = 34/75 (45%), Gaps = 6/75 (8%) Query: 13 IFVTKEDHET---PSSAELVADDPNDPYEEHGLILPNGNINWNCPCLGGMASGPCGEQF- 68 + +++ +H+ +SA + DP+ +EE + + + P + + P G +F Sbjct: 48 LHISRTEHQIVADNTSAWIEVTDPDALHEEWARAVSTDYADTSGPAMTPVGESPAGREFA 107 Query: 69 --KSAFSCFHYSTEE 81 A +C H++ E Sbjct: 108 VRDPAGNCVHFTAGE 122 >6GAW-Bv nrPDB Length = 222 Score = 27.0 bits (58), Expect = 2.8, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 27/45 (60%), Gaps = 1/45 (2%) Query: 92 RAMQECMQKYPDLYPQEDEDEEEEREKKPAEQAEETAPIEATATK 136 + ++ECM K P + + ++E REK+ A++ + A ++A A + Sbjct: 119 QLIEECMAKMPQMIENWRQQQQERREKEQADK-DRRARLQAEAQE 162 >1B9C-A nrPDB Length = 238 Score = 26.6 bits (57), Expect = 3.6, Method: Composition-based stats. Identities = 8/22 (36%), Positives = 10/22 (45%) Query: 88 VDQFRAMQECMQKYPDLYPQED 109 V F +C +YPD Q D Sbjct: 61 VTTFTYGVQCFSRYPDHMKQHD 82 >3NMS-C nrPDB Length = 343 Score = 26.2 bits (56), Expect = 5.5, Method: Composition-based stats. Identities = 7/17 (41%), Positives = 14/17 (82%) Query: 103 DLYPQEDEDEEEEREKK 119 + +P+EDE ++EE +K+ Sbjct: 309 EHWPEEDECQDEENQKQ 325 >6GMH-I nrPDB Length = 125 Score = 26.2 bits (56), Expect = 5.6, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 17/32 (53%) Query: 5 RQEGKDRIIFVTKEDHETPSSAELVADDPNDP 36 +QE + I+V K HE +++AD DP Sbjct: 45 QQEADNSCIYVNKITHEVDELTQIIADVSQDP 76 >4CE4-u nrPDB Length = 205 Score = 25.8 bits (55), Expect = 6.7, Method: Composition-based stats. Identities = 13/38 (34%), Positives = 21/38 (55%), Gaps = 1/38 (2%) Query: 78 STEEIKGSDCVD-QFRAMQECMQKYPDLYPQEDEDEEE 114 S E I S+C QFR + +C+QK D+ + + +E Sbjct: 129 SGELILTSECSRYQFRNLADCLQKLRDMIAEASQPAKE 166 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.306 0.131 0.389 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,473,222 Number of Sequences: 36641 Number of extensions: 286099 Number of successful extensions: 1119 Number of sequences better than 10.0: 34 Number of HSP's better than 10.0 without gapping: 58 Number of HSP's successfully gapped in prelim test: 26 Number of HSP's that attempted gapping in prelim test: 958 Number of HSP's gapped (non-prelim): 161 length of query: 142 length of database: 10,336,785 effective HSP length: 87 effective length of query: 55 effective length of database: 7,149,018 effective search space: 393195990 effective search space used: 393195990 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.5 bits) S2: 54 (25.4 bits)