BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8JFY9 (292 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2C1W-A nrPDB 609 e-175 1LUF-A nrPDB 33 0.21 5CG0-A nrPDB 32 0.48 4UN1-A nrPDB 29 2.9 4OO1-C nrPDB 28 4.9 2BIF-A nrPDB 27 8.7 5C9S-B nrPDB 27 9.6 >2C1W-A nrPDB Length = 292 Score = 609 bits (1571), Expect = e-175, Method: Compositional matrix adjust. Identities = 292/292 (100%), Positives = 292/292 (100%) Query: 1 MASNRGQLNHELSKLFNELWDADQNRMKSGKDYRISLQGKAGYVPAGSNQARDSASFPLF 60 MASNRGQLNHELSKLFNELWDADQNRMKSGKDYRISLQGKAGYVPAGSNQARDSASFPLF Sbjct: 1 MASNRGQLNHELSKLFNELWDADQNRMKSGKDYRISLQGKAGYVPAGSNQARDSASFPLF 60 Query: 61 QFVDEEKLKSRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHD 120 QFVDEEKLKSRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHD Sbjct: 61 QFVDEEKLKSRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHD 120 Query: 121 YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNW 180 YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNW Sbjct: 121 YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNW 180 Query: 181 VQFYLQEKRKNIDYKGYVARQNKSRPDEDDQVLNLQFNWKEMVKPVGSSFIGVSPEFEFA 240 VQFYLQEKRKNIDYKGYVARQNKSRPDEDDQVLNLQFNWKEMVKPVGSSFIGVSPEFEFA Sbjct: 181 VQFYLQEKRKNIDYKGYVARQNKSRPDEDDQVLNLQFNWKEMVKPVGSSFIGVSPEFEFA 240 Query: 241 LYTIVFLASQEKMSREVVRLEEYELQIVVNRHGRYIGTAYPVLLSTNNPDLY 292 LYTIVFLASQEKMSREVVRLEEYELQIVVNRHGRYIGTAYPVLLSTNNPDLY Sbjct: 241 LYTIVFLASQEKMSREVVRLEEYELQIVVNRHGRYIGTAYPVLLSTNNPDLY 292 >1LUF-A nrPDB Length = 343 Score = 32.7 bits (73), Expect = 0.21, Method: Compositional matrix adjust. Identities = 16/64 (25%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Query: 117 MAHD---YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQE 173 MAH+ Y VR + ++LYN+ +S+ P RP C + +R + Sbjct: 280 MAHEEVIYYVRDGNILACPENCPLELYNLMRLCWSKLPADRPSFCSIHRILQRMCERAEG 339 Query: 174 MMGL 177 +G+ Sbjct: 340 TVGV 343 >5CG0-A nrPDB Length = 489 Score = 31.6 bits (70), Expect = 0.48, Method: Compositional matrix adjust. Identities = 19/64 (29%), Positives = 30/64 (46%) Query: 86 MDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHDYLVRKNQAKPTRNDFKVQLYNIWFQ 145 + TG+A V P IA NN++D +L+ + + Y Q F L + WF+ Sbjct: 86 LPTGMANEVNPAGIAFYNNYIDEMLKYNITPLITLYHWDLPQKLQELGGFANPLISDWFE 145 Query: 146 LYSR 149 Y+R Sbjct: 146 DYAR 149 >4UN1-A nrPDB Length = 173 Score = 28.9 bits (63), Expect = 2.9, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 2/46 (4%) Query: 105 FLDAILETKVMKMAHDYLVRKNQAKP--TRNDFKVQLYNIWFQLYS 148 F+ + KV + D V + AKP T N + YNIWF L S Sbjct: 86 FVSTLCAAKVPQDKMDAFVAEVNAKPGVTHNYLREHDYNIWFTLIS 131 >4OO1-C nrPDB Length = 394 Score = 28.1 bits (61), Expect = 4.9, Method: Compositional matrix adjust. Identities = 26/96 (27%), Positives = 43/96 (44%), Gaps = 11/96 (11%) Query: 24 QNRMKSGKDYRI-SLQGKAGYVPAGSNQARDSASF---PLFQFVDEEKLKSRKTFATFIS 79 N +KSGK I S+ G G + S +D F LF+ EE + A + S Sbjct: 77 SNVLKSGKTIVITSITG--GIIEETSAAIKDLDDFGEEELFEVTKEEDI-----IANYAS 129 Query: 80 LLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVM 115 + E++ G T EE+ + D+IL ++++ Sbjct: 130 VYPVVEVERGRVGACTDEEMTISQKLHDSILHSRIL 165 >2BIF-A nrPDB Length = 469 Score = 27.3 bits (59), Expect = 8.7, Method: Compositional matrix adjust. Identities = 24/83 (28%), Positives = 40/83 (48%), Gaps = 16/83 (19%) Query: 35 ISLQGKAGYVPAGSNQARDSASFPLFQFVDEEKLKSRKTFA--------TFISLLDNYE- 85 ++L+G+ G P S + R+ + L QF+ ++ +K K F T +L YE Sbjct: 262 LNLKGRIGGDPGLSPRGREFSKH-LAQFISDQNIKDLKVFTSQMKRTIQTAEALSVPYEQ 320 Query: 86 ------MDTGVAEVVTPEEIAEN 102 +D GV E +T EEI ++ Sbjct: 321 FKVLNEIDAGVCEEMTYEEIQDH 343 >5C9S-B nrPDB Length = 572 Score = 26.9 bits (58), Expect = 9.6, Method: Compositional matrix adjust. Identities = 19/79 (24%), Positives = 32/79 (40%), Gaps = 6/79 (7%) Query: 136 KVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNWVQFYLQE-KRKNIDY 194 ++ L+N++ +A + FE + + R Q + W+QF E K I+Y Sbjct: 494 RIDLWNVYVDQEVKAKDKKKVEDLFERIITKKITRKQAKFFFNKWLQFEESEGDEKTIEY 553 Query: 195 -----KGYVARQNKSRPDE 208 YVA + DE Sbjct: 554 VKAKATEYVASHESQKADE 572 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2C1W-A nrPDB 631 0.0 Sequences not found previously or not previously below threshold: 5CG0-A nrPDB 32 0.40 1LUF-A nrPDB 31 0.80 4UN1-A nrPDB 29 2.2 5C9S-B nrPDB 28 3.2 1JMC-A nrPDB 28 4.5 4OO1-C nrPDB 28 4.7 2BIF-A nrPDB 28 5.1 4D4L-A nrPDB 28 6.7 2DWP-A nrPDB 28 6.7 CONVERGED! >2C1W-A nrPDB Length = 292 Score = 631 bits (1627), Expect = 0.0, Method: Composition-based stats. Identities = 292/292 (100%), Positives = 292/292 (100%) Query: 1 MASNRGQLNHELSKLFNELWDADQNRMKSGKDYRISLQGKAGYVPAGSNQARDSASFPLF 60 MASNRGQLNHELSKLFNELWDADQNRMKSGKDYRISLQGKAGYVPAGSNQARDSASFPLF Sbjct: 1 MASNRGQLNHELSKLFNELWDADQNRMKSGKDYRISLQGKAGYVPAGSNQARDSASFPLF 60 Query: 61 QFVDEEKLKSRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHD 120 QFVDEEKLKSRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHD Sbjct: 61 QFVDEEKLKSRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHD 120 Query: 121 YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNW 180 YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNW Sbjct: 121 YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNW 180 Query: 181 VQFYLQEKRKNIDYKGYVARQNKSRPDEDDQVLNLQFNWKEMVKPVGSSFIGVSPEFEFA 240 VQFYLQEKRKNIDYKGYVARQNKSRPDEDDQVLNLQFNWKEMVKPVGSSFIGVSPEFEFA Sbjct: 181 VQFYLQEKRKNIDYKGYVARQNKSRPDEDDQVLNLQFNWKEMVKPVGSSFIGVSPEFEFA 240 Query: 241 LYTIVFLASQEKMSREVVRLEEYELQIVVNRHGRYIGTAYPVLLSTNNPDLY 292 LYTIVFLASQEKMSREVVRLEEYELQIVVNRHGRYIGTAYPVLLSTNNPDLY Sbjct: 241 LYTIVFLASQEKMSREVVRLEEYELQIVVNRHGRYIGTAYPVLLSTNNPDLY 292 >5CG0-A nrPDB Length = 489 Score = 31.6 bits (70), Expect = 0.40, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 30/64 (46%) Query: 86 MDTGVAEVVTPEEIAENNNFLDAILETKVMKMAHDYLVRKNQAKPTRNDFKVQLYNIWFQ 145 + TG+A V P IA NN++D +L+ + + Y Q F L + WF+ Sbjct: 86 LPTGMANEVNPAGIAFYNNYIDEMLKYNITPLITLYHWDLPQKLQELGGFANPLISDWFE 145 Query: 146 LYSR 149 Y+R Sbjct: 146 DYAR 149 >1LUF-A nrPDB Length = 343 Score = 30.8 bits (68), Expect = 0.80, Method: Composition-based stats. Identities = 16/64 (25%), Positives = 28/64 (43%), Gaps = 3/64 (4%) Query: 117 MAHD---YLVRKNQAKPTRNDFKVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQE 173 MAH+ Y VR + ++LYN+ +S+ P RP C + +R + Sbjct: 280 MAHEEVIYYVRDGNILACPENCPLELYNLMRLCWSKLPADRPSFCSIHRILQRMCERAEG 339 Query: 174 MMGL 177 +G+ Sbjct: 340 TVGV 343 >4UN1-A nrPDB Length = 173 Score = 29.3 bits (64), Expect = 2.2, Method: Composition-based stats. Identities = 17/46 (36%), Positives = 22/46 (47%), Gaps = 2/46 (4%) Query: 105 FLDAILETKVMKMAHDYLVRKNQAKP--TRNDFKVQLYNIWFQLYS 148 F+ + KV + D V + AKP T N + YNIWF L S Sbjct: 86 FVSTLCAAKVPQDKMDAFVAEVNAKPGVTHNYLREHDYNIWFTLIS 131 >5C9S-B nrPDB Length = 572 Score = 28.5 bits (62), Expect = 3.2, Method: Composition-based stats. Identities = 19/79 (24%), Positives = 32/79 (40%), Gaps = 6/79 (7%) Query: 136 KVQLYNIWFQLYSRAPGSRPDSCGFEHVFVGESKRGQEMMGLHNWVQFYLQE-KRKNIDY 194 ++ L+N++ +A + FE + + R Q + W+QF E K I+Y Sbjct: 494 RIDLWNVYVDQEVKAKDKKKVEDLFERIITKKITRKQAKFFFNKWLQFEESEGDEKTIEY 553 Query: 195 -----KGYVARQNKSRPDE 208 YVA + DE Sbjct: 554 VKAKATEYVASHESQKADE 572 >1JMC-A nrPDB Length = 246 Score = 28.1 bits (61), Expect = 4.5, Method: Composition-based stats. Identities = 22/97 (22%), Positives = 41/97 (42%), Gaps = 15/97 (15%) Query: 14 KLFNELWDADQNRMKSGKDYRISLQGKAGYVPAGSNQARDSASFPLFQF----VDEEKLK 69 K+ N+ + A +N DY ++ + +P D P QF +D+ + K Sbjct: 87 KIANKQFTAVKN------DYEMTFNNETSVMPC-----EDDHHLPTVQFDFTGIDDLENK 135 Query: 70 SRKTFATFISLLDNYEMDTGVAEVVTPEEIAENNNFL 106 S+ + I + +YE T + E+A+ N +L Sbjct: 136 SKDSLVDIIGICKSYEDATKITVRSNNREVAKRNIYL 172 >4OO1-C nrPDB Length = 394 Score = 28.1 bits (61), Expect = 4.7, Method: Composition-based stats. Identities = 26/95 (27%), Positives = 43/95 (45%), Gaps = 11/95 (11%) Query: 25 NRMKSGKDYRI-SLQGKAGYVPAGSNQARDSASF---PLFQFVDEEKLKSRKTFATFISL 80 N +KSGK I S+ G G + S +D F LF+ EE + A + S+ Sbjct: 78 NVLKSGKTIVITSITG--GIIEETSAAIKDLDDFGEEELFEVTKEEDI-----IANYASV 130 Query: 81 LDNYEMDTGVAEVVTPEEIAENNNFLDAILETKVM 115 E++ G T EE+ + D+IL ++++ Sbjct: 131 YPVVEVERGRVGACTDEEMTISQKLHDSILHSRIL 165 >2BIF-A nrPDB Length = 469 Score = 28.1 bits (61), Expect = 5.1, Method: Composition-based stats. Identities = 23/83 (27%), Positives = 41/83 (49%), Gaps = 16/83 (19%) Query: 35 ISLQGKAGYVPAGSNQARDSASFPLFQFVDEEKLKSRKTFATFI--------SLLDNYE- 85 ++L+G+ G P S + R+ + L QF+ ++ +K K F + + +L YE Sbjct: 262 LNLKGRIGGDPGLSPRGREFSKH-LAQFISDQNIKDLKVFTSQMKRTIQTAEALSVPYEQ 320 Query: 86 ------MDTGVAEVVTPEEIAEN 102 +D GV E +T EEI ++ Sbjct: 321 FKVLNEIDAGVCEEMTYEEIQDH 343 >4D4L-A nrPDB Length = 449 Score = 27.7 bits (60), Expect = 6.7, Method: Composition-based stats. Identities = 26/81 (32%), Positives = 39/81 (48%), Gaps = 16/81 (19%) Query: 36 SLQGKAGYVPAGSNQARDSASFPLFQFVDEEKLKS--------RKTFATFISLLDNYE-- 85 +LQG+ G S++ + AS L +FV+E+ LK + T T +L YE Sbjct: 260 NLQGRIGGDSGLSSRGKKFAS-ALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQW 318 Query: 86 -----MDTGVAEVVTPEEIAE 101 +D GV E +T EEI + Sbjct: 319 KALNEIDAGVCEELTYEEIRD 339 >2DWP-A nrPDB Length = 520 Score = 27.7 bits (60), Expect = 6.7, Method: Composition-based stats. Identities = 26/81 (32%), Positives = 39/81 (48%), Gaps = 16/81 (19%) Query: 36 SLQGKAGYVPAGSNQARDSASFPLFQFVDEEKLKS--------RKTFATFISLLDNYE-- 85 +LQG+ G S++ + AS L +FV+E+ LK + T T +L YE Sbjct: 260 NLQGRIGGDSGLSSRGKKFAS-ALSKFVEEQNLKDLRVWTSQLKSTIQTAEALRLPYEQW 318 Query: 86 -----MDTGVAEVVTPEEIAE 101 +D GV E +T EEI + Sbjct: 319 KALNEIDAGVCEELTYEEIRD 339 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.134 0.392 Lambda K H 0.267 0.0412 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,126,774 Number of Sequences: 36641 Number of extensions: 496783 Number of successful extensions: 1211 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 1203 Number of HSP's gapped (non-prelim): 24 length of query: 292 length of database: 10,336,785 effective HSP length: 96 effective length of query: 196 effective length of database: 6,819,249 effective search space: 1336572804 effective search space used: 1336572804 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 59 (27.3 bits)