BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8IV48 (349 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1ZBU-A nrPDB 724 0.0 3CG7-A nrPDB 98 4e-21 5NLY-A nrPDB 32 0.28 2P1J-A nrPDB 32 0.33 5UYS-A nrPDB 29 3.6 >1ZBU-A nrPDB Length = 349 Score = 724 bits (1868), Expect = 0.0, Method: Compositional matrix adjust. Identities = 348/349 (99%), Positives = 348/349 (99%) Query: 1 MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASD 60 MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASD Sbjct: 1 MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASD 60 Query: 61 FSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKES 120 FSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKES Sbjct: 61 FSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKES 120 Query: 121 NFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINT 180 NFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINT Sbjct: 121 NFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINT 180 Query: 181 QLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKFLN 240 QLSDFCISLTGITQDQVDRADTFPQVLKKVID MKLKELGTKYKYSLLTDGSWDMSKFLN Sbjct: 181 QLSDFCISLTGITQDQVDRADTFPQVLKKVIDLMKLKELGTKYKYSLLTDGSWDMSKFLN 240 Query: 241 IQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSK 300 IQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSK Sbjct: 241 IQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSK 300 Query: 301 NIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK 349 NIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK Sbjct: 301 NIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK 349 >3CG7-A nrPDB Length = 308 Score = 98.2 bits (243), Expect = 4e-21, Method: Compositional matrix adjust. Identities = 63/204 (30%), Positives = 109/204 (53%), Gaps = 11/204 (5%) Query: 127 YDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIED--TFQQYVRPEINTQLSD 184 +D + I+DFE T + N ++ E+I+F +V + +I + +F +YV+P +N L+ Sbjct: 18 FDTLLILDFETTSDAANQ-DYPCEVIQFAIVAYDVPNDKIREDISFNKYVKPVLNRTLTK 76 Query: 185 FCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKY-KYSLLTDGSWDMSKFLNIQC 243 C+ TGI Q +D ADTF V ++ W L LG + K++ + D D+ + Q Sbjct: 77 NCVDFTGIPQRSIDTADTFDVVYEQFQQW--LITLGLEEGKFAFVCDSRQDLWRIAQYQM 134 Query: 244 QLSRLKYPPFAKKWINIRKSYGN-FYKVPRSQTKLTIMLEKLGMDYD----GRPHCGLDD 298 +LS ++ P F +++IN+ K + N ++ + T + K+ YD GR H +DD Sbjct: 135 KLSNIQMPAFFRQYINLYKIFTNEMDRMGPKELSATTNIGKMNEYYDLPTIGRAHDAMDD 194 Query: 299 SKNIARIAVRMLQDGCELRINEKM 322 NIA I RM+ G ++ +NE + Sbjct: 195 CLNIATILQRMINMGAKVTVNELL 218 >5NLY-A nrPDB Length = 405 Score = 32.3 bits (72), Expect = 0.28, Method: Compositional matrix adjust. Identities = 32/144 (22%), Positives = 63/144 (43%), Gaps = 13/144 (9%) Query: 78 RMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFEA 137 + +++ L + E T + + L+K + + + Q K +++ CI + Sbjct: 104 QFNRDVLDCVVDEMDFSTMELDEALRK-FQAHIRVQGEAQKVERLIEAFSQRYCICNPGV 162 Query: 138 TCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINTQLSDFCISLTGITQDQV 197 + NP I+ F ++LLNT D + V+PE +L DF +L G+ Sbjct: 163 VRQFRNPDTIF--ILAFAIILLNT------DMYSPNVKPERKMKLEDFIKNLRGVD---- 210 Query: 198 DRADTFPQVLKKVIDWMKLKELGT 221 D D ++L + + ++ +EL T Sbjct: 211 DGEDIPREMLMGIYERIRKRELKT 234 >2P1J-A nrPDB Length = 186 Score = 32.3 bits (72), Expect = 0.33, Method: Compositional matrix adjust. Identities = 48/206 (23%), Positives = 89/206 (43%), Gaps = 50/206 (24%) Query: 119 ESNFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEI 178 +S F D+ + ++DFE T G P+ V EIIE V + +I D + ++P Sbjct: 6 DSTFGDATF---VVLDFETT---GLDPQ-VDEIIEIGAVKIQGG--QIVDEYHTLIKP-- 54 Query: 179 NTQLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKF 238 + ++S +TGITQ+ ++ + +VL + + ++ + S ++ Sbjct: 55 SREISRKSSEITGITQEMLENKRSIEEVLPEFLGFL---------------EDSIIVAHN 99 Query: 239 LNIQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQT-------------KLTIMLEKLG 285 N + RL W I+K G ++ P T L ++EKLG Sbjct: 100 ANFDYRFLRL--------W--IKKVMGLDWERPYIDTLALAKSLLKLRSYSLDSVVEKLG 149 Query: 286 MDYDGRPHCGLDDSKNIARIAVRMLQ 311 + R H LDD++ A++ +R ++ Sbjct: 150 LG-PFRHHRALDDARVTAQVFLRFVE 174 >5UYS-A nrPDB Length = 494 Score = 28.9 bits (63), Expect = 3.6, Method: Compositional matrix adjust. Identities = 29/117 (24%), Positives = 47/117 (40%), Gaps = 21/117 (17%) Query: 217 KELGTKYKYSLLTDGSWDMSKFLNIQCQLSRL-KYPPFAKKWINIRKSYGNFYKVPRSQT 275 K+ G KY SLL+ L + L L ++ + ++K YG Y V R T Sbjct: 3 KKTGAKYPKSLLS---------LPLVGSLPFLPRHGHMHNNFFKLQKKYGPIYSV-RMGT 52 Query: 276 KLTIM----------LEKLGMDYDGRPHCGLDDSKNIARIAVRMLQDGCELRINEKM 322 K T++ L K G D+ GRP D + R + G +++ ++ Sbjct: 53 KTTVIVGHHQLAKEVLIKKGKDFSGRPQMATLDIASNNRKGIAFADSGAHWQLHRRL 109 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1ZBU-A nrPDB 667 0.0 3CG7-A nrPDB 310 6e-85 Sequences not found previously or not previously below threshold: 2P1J-A nrPDB 46 3e-05 6G3X-A nrPDB 32 0.34 4CY4-A nrPDB 29 2.4 3DRH-A nrPDB 29 3.0 4EGW-A nrPDB 29 3.3 4CCW-A nrPDB 28 8.0 1A76-A nrPDB 27 9.2 >1ZBU-A nrPDB Length = 349 Score = 667 bits (1720), Expect = 0.0, Method: Composition-based stats. Identities = 348/349 (99%), Positives = 348/349 (99%) Query: 1 MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASD 60 MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASD Sbjct: 1 MEDPQSKEPAGEAVALALLESPRPEGGEEPPRPSPEETQQCKFDGQETKGSKFITSSASD 60 Query: 61 FSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKES 120 FSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKES Sbjct: 61 FSDPVYKEIAITNGCINRMSKEELRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKES 120 Query: 121 NFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINT 180 NFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINT Sbjct: 121 NFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEINT 180 Query: 181 QLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKFLN 240 QLSDFCISLTGITQDQVDRADTFPQVLKKVID MKLKELGTKYKYSLLTDGSWDMSKFLN Sbjct: 181 QLSDFCISLTGITQDQVDRADTFPQVLKKVIDLMKLKELGTKYKYSLLTDGSWDMSKFLN 240 Query: 241 IQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSK 300 IQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSK Sbjct: 241 IQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDDSK 300 Query: 301 NIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK 349 NIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK Sbjct: 301 NIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPPPQMPHFRK 349 >3CG7-A nrPDB Length = 308 Score = 310 bits (794), Expect = 6e-85, Method: Composition-based stats. Identities = 63/204 (30%), Positives = 109/204 (53%), Gaps = 11/204 (5%) Query: 127 YDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIED--TFQQYVRPEINTQLSD 184 +D + I+DFE T + N ++ E+I+F +V + +I + +F +YV+P +N L+ Sbjct: 18 FDTLLILDFETTSDAANQ-DYPCEVIQFAIVAYDVPNDKIREDISFNKYVKPVLNRTLTK 76 Query: 185 FCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKY-KYSLLTDGSWDMSKFLNIQC 243 C+ TGI Q +D ADTF V ++ W L LG + K++ + D D+ + Q Sbjct: 77 NCVDFTGIPQRSIDTADTFDVVYEQFQQW--LITLGLEEGKFAFVCDSRQDLWRIAQYQM 134 Query: 244 QLSRLKYPPFAKKWINIRKSYGN-FYKVPRSQTKLTIMLEKLGMDYD----GRPHCGLDD 298 +LS ++ P F +++IN+ K + N ++ + T + K+ YD GR H +DD Sbjct: 135 KLSNIQMPAFFRQYINLYKIFTNEMDRMGPKELSATTNIGKMNEYYDLPTIGRAHDAMDD 194 Query: 299 SKNIARIAVRMLQDGCELRINEKM 322 NIA I RM+ G ++ +NE + Sbjct: 195 CLNIATILQRMINMGAKVTVNELL 218 >2P1J-A nrPDB Length = 186 Score = 45.8 bits (107), Expect = 3e-05, Method: Composition-based stats. Identities = 43/194 (22%), Positives = 89/194 (45%), Gaps = 24/194 (12%) Query: 119 ESNFADSYYDYICIIDFEATCEEGNPPEFVHEIIEFPVVLLNTHTLEIEDTFQQYVRPEI 178 +S F D+ + ++DFE T G P+ V EIIE + + +I D + ++P Sbjct: 6 DSTFGDATF---VVLDFETT---GLDPQ-VDEIIE--IGAVKIQGGQIVDEYHTLIKP-- 54 Query: 179 NTQLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGTKYKYSLLTDGSWDMSKF 238 + ++S +TGITQ+ ++ + +VL + LG ++ + +F Sbjct: 55 SREISRKSSEITGITQEMLENKRSIEEVLPEF--------LGFLEDSIIVAHNANFDYRF 106 Query: 239 LNIQCQLSRLKYPPFAKKWINIRKSYGNFYKVPRSQTKLTIMLEKLGMDYDGRPHCGLDD 298 L + + ++ + + +I+ + K+ L ++EKLG+ R H LDD Sbjct: 107 LRLWIK--KVMGLDWERPYIDTLALAKSLLKL--RSYSLDSVVEKLGLG-PFRHHRALDD 161 Query: 299 SKNIARIAVRMLQD 312 ++ A++ +R ++ Sbjct: 162 ARVTAQVFLRFVEM 175 >6G3X-A nrPDB Length = 318 Score = 32.3 bits (72), Expect = 0.34, Method: Composition-based stats. Identities = 23/134 (17%), Positives = 51/134 (38%), Gaps = 19/134 (14%) Query: 162 HTLEIEDTFQQYVRPEINTQLSDFCISLTGITQDQVDRADTFPQVLKKVIDWMKLKELGT 221 TLE +T +Y + + +SL + F +V +K L++ T Sbjct: 81 PTLEELETLHKYFQLD---------VSLAQLYSHWASVDSHFQRVAQKFQGVRLLRQDPT 131 Query: 222 KYKYSLLTDGSWDMSKFLNIQCQLSRLKYPPFAKKWINI----RKSYGNFYKVPRSQTKL 277 + +S + + ++++ + +L + F + I + + N + + + Sbjct: 132 ECLFSFICSSNNNIARITGMVERLCQ----AFGPRLIQLDDVTYHGFPNLHALAGPE--A 185 Query: 278 TIMLEKLGMDYDGR 291 L KLG+ Y R Sbjct: 186 ETHLRKLGLGYRAR 199 >4CY4-A nrPDB Length = 317 Score = 29.2 bits (64), Expect = 2.4, Method: Composition-based stats. Identities = 21/58 (36%), Positives = 24/58 (41%), Gaps = 1/58 (1%) Query: 84 LRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFEATCEE 141 LR L F +DVL LK Y NF D YYD + +ID AT E Sbjct: 180 LRKTLVYFHKSLIANRDVLV-LLKRKYLPITTKEDRENFEDLYYDTLQLIDMSATYRE 236 >3DRH-A nrPDB Length = 590 Score = 29.2 bits (64), Expect = 3.0, Method: Composition-based stats. Identities = 25/119 (21%), Positives = 42/119 (35%), Gaps = 19/119 (15%) Query: 237 KFLNIQCQLSRLKYPPFAKKWI----------NIRKSYGNFYKVPRSQTKLTIMLEK--- 283 KF N + PP K++ ++ K+ + K T EK Sbjct: 348 KFSNGLSTPANSLIPPIFKQFTSSSVKGYEKQDLDKANKLLDEDGWKLNKSTGYREKDGK 407 Query: 284 -LGMDYDGRPHCGLDDSKNIARIAVRMLQDGCELRINEKMHAGQLMSVSSSLPIEGTPP 341 L + Y R N IA +Q ++ + ++ G+LM +S + TPP Sbjct: 408 ELSLVYAARVGDA-----NAETIAQNYIQQWKKIGVKVSLYNGKLMEFNSWVDHMTTPP 461 >4EGW-A nrPDB Length = 280 Score = 28.9 bits (63), Expect = 3.3, Method: Composition-based stats. Identities = 21/58 (36%), Positives = 24/58 (41%), Gaps = 1/58 (1%) Query: 84 LRAKLSEFKLETRGVKDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFEATCEE 141 LR L F +DVL LK Y NF D YYD + +ID AT E Sbjct: 202 LRKTLVYFHKSLIANRDVLV-LLKRKYLPITTKEDRENFEDLYYDTLQLIDMSATYRE 258 >4CCW-A nrPDB Length = 299 Score = 27.7 bits (60), Expect = 8.0, Method: Composition-based stats. Identities = 12/35 (34%), Positives = 18/35 (51%) Query: 223 YKYSLLTDGSWDMSKFLNIQCQLSRLKYPPFAKKW 257 YKY+L S + KFLN + +P F K++ Sbjct: 166 YKYALGLTASNGVEKFLNWMMTDQNVLHPIFVKQF 200 >1A76-A nrPDB Length = 326 Score = 27.3 bits (59), Expect = 9.2, Method: Composition-based stats. Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 2/47 (4%) Query: 91 FKLETRGV-KDVLKKRLKNYYKKQKLMLKESNFADSYYDYICIIDFE 136 ++L GV KDVLKK ++ YY + K + KE D+Y + + D E Sbjct: 239 YELVRSGVAKDVLKKEVE-YYDEIKRIFKEPKVTDNYSLSLKLPDKE 284 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.135 0.388 Lambda K H 0.267 0.0400 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,820,652 Number of Sequences: 36641 Number of extensions: 551112 Number of successful extensions: 1494 Number of sequences better than 10.0: 12 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 1471 Number of HSP's gapped (non-prelim): 26 length of query: 349 length of database: 10,336,785 effective HSP length: 98 effective length of query: 251 effective length of database: 6,745,967 effective search space: 1693237717 effective search space used: 1693237717 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 59 (27.4 bits)