BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8IU54 (200 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3OG4-A nrPDB 363 e-101 3HHC-A nrPDB 270 3e-73 5T5W-C nrPDB 255 8e-69 6EML-S nrPDB 27 6.7 3JAM-E nrPDB 27 7.0 >3OG4-A nrPDB Length = 178 Score = 363 bits (931), Expect = e-101, Method: Compositional matrix adjust. Identities = 178/178 (100%), Positives = 178/178 (100%) Query: 23 PTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLL 82 PTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLL Sbjct: 1 PTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLL 60 Query: 83 QVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRP 142 QVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRP Sbjct: 61 QVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRP 120 Query: 143 RGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST 200 RGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST Sbjct: 121 RGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST 178 >3HHC-A nrPDB Length = 196 Score = 270 bits (691), Expect = 3e-73, Method: Compositional matrix adjust. Identities = 140/189 (74%), Positives = 153/189 (80%), Gaps = 5/189 (2%) Query: 8 VLVTLVLGLAVAGPVPTSK---PTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLK 64 VLV + L V G VP ++ +GCHI +FKSLSPQEL +FK+A+DALEESL LK Sbjct: 8 VLVLMAAVLTVTGAVPVARLRGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLK 67 Query: 65 NWSCSSPVFPGNWDLRLLQVRERPVALEAELALTLKVLEAAAG--PALEDVLDQPLHTLH 122 + C S +FP WDLR LQVRERPVALEAELALTLKVLEA A PAL DVLDQPLHTLH Sbjct: 68 DCKCRSRLFPRTWDLRQLQVRERPVALEAELALTLKVLEATADTDPALGDVLDQPLHTLH 127 Query: 123 HILSQLQACIQPQPTAGPRPRGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLK 182 HILSQL+ACIQPQPTAGPR RGRLHHWLHRLQEAPKKES GCLEASVTFNLFRLLTRDL Sbjct: 128 HILSQLRACIQPQPTAGPRTRGRLHHWLHRLQEAPKKESPGCLEASVTFNLFRLLTRDLN 187 Query: 183 YVADGNLCL 191 VA G+LC+ Sbjct: 188 CVASGDLCV 196 >5T5W-C nrPDB Length = 176 Score = 255 bits (652), Expect = 8e-69, Method: Compositional matrix adjust. Identities = 128/163 (78%), Positives = 140/163 (85%), Gaps = 2/163 (1%) Query: 30 TGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLLQVRERPV 89 + +GCHI +FKSLSP+EL +FK+A+DALEESL LK+ C S +FP WDLR LQVRERPV Sbjct: 2 SARGCHIAQFKSLSPRELQAFKRAKDALEESLLLKDCKCRSRLFPRTWDLRQLQVRERPV 61 Query: 90 ALEAELALTLKVLEAAAG--PALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRPRGRLH 147 ALEAELALTLKVL+A A PAL DVLDQPLHTLHHILSQL+ACIQPQPTAGPR RGRLH Sbjct: 62 ALEAELALTLKVLDATADTDPALGDVLDQPLHTLHHILSQLRACIQPQPTAGPRTRGRLH 121 Query: 148 HWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLC 190 WLHRLQEAPKKES GCLEASVTFNLFRLL RDL VA G+LC Sbjct: 122 RWLHRLQEAPKKESPGCLEASVTFNLFRLLARDLNCVASGDLC 164 >6EML-S nrPDB Length = 261 Score = 26.6 bits (57), Expect = 6.7, Method: Compositional matrix adjust. Identities = 13/36 (36%), Positives = 20/36 (55%), Gaps = 11/36 (30%) Query: 122 HH-ILSQLQACIQPQPTAGPRPRGRLHHWLHRLQEA 156 HH +L +L C P+P+AGP H+L+E+ Sbjct: 16 HHWLLDKLSGCYAPRPSAGP----------HKLRES 41 >3JAM-E nrPDB Length = 261 Score = 26.6 bits (57), Expect = 7.0, Method: Compositional matrix adjust. Identities = 9/19 (47%), Positives = 13/19 (68%) Query: 122 HHILSQLQACIQPQPTAGP 140 H +L +L C P+P+AGP Sbjct: 17 HWMLDKLSGCYAPRPSAGP 35 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3HHC-A nrPDB 321 2e-88 3OG4-A nrPDB 320 4e-88 5T5W-C nrPDB 293 4e-80 Sequences not found previously or not previously below threshold: 4ZN6-A nrPDB 30 0.64 6EML-S nrPDB 29 1.6 3JAM-E nrPDB 28 1.8 5ZHY-A nrPDB 28 3.4 2CST-A nrPDB 27 5.9 5YL9-A nrPDB 27 6.8 2X19-B nrPDB 26 9.2 CONVERGED! >3HHC-A nrPDB Length = 196 Score = 321 bits (822), Expect = 2e-88, Method: Composition-based stats. Identities = 140/189 (74%), Positives = 153/189 (80%), Gaps = 5/189 (2%) Query: 8 VLVTLVLGLAVAGPVPTSK---PTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLK 64 VLV + L V G VP ++ +GCHI +FKSLSPQEL +FK+A+DALEESL LK Sbjct: 8 VLVLMAAVLTVTGAVPVARLRGALPDARGCHIAQFKSLSPQELQAFKRAKDALEESLLLK 67 Query: 65 NWSCSSPVFPGNWDLRLLQVRERPVALEAELALTLKVLEAAAG--PALEDVLDQPLHTLH 122 + C S +FP WDLR LQVRERPVALEAELALTLKVLEA A PAL DVLDQPLHTLH Sbjct: 68 DCKCRSRLFPRTWDLRQLQVRERPVALEAELALTLKVLEATADTDPALGDVLDQPLHTLH 127 Query: 123 HILSQLQACIQPQPTAGPRPRGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLK 182 HILSQL+ACIQPQPTAGPR RGRLHHWLHRLQEAPKKES GCLEASVTFNLFRLLTRDL Sbjct: 128 HILSQLRACIQPQPTAGPRTRGRLHHWLHRLQEAPKKESPGCLEASVTFNLFRLLTRDLN 187 Query: 183 YVADGNLCL 191 VA G+LC+ Sbjct: 188 CVASGDLCV 196 >3OG4-A nrPDB Length = 178 Score = 320 bits (819), Expect = 4e-88, Method: Composition-based stats. Identities = 178/178 (100%), Positives = 178/178 (100%) Query: 23 PTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLL 82 PTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLL Sbjct: 1 PTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLL 60 Query: 83 QVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRP 142 QVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRP Sbjct: 61 QVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRP 120 Query: 143 RGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST 200 RGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST Sbjct: 121 RGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLCLRTSTHPEST 178 >5T5W-C nrPDB Length = 176 Score = 293 bits (750), Expect = 4e-80, Method: Composition-based stats. Identities = 128/163 (78%), Positives = 140/163 (85%), Gaps = 2/163 (1%) Query: 30 TGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPVFPGNWDLRLLQVRERPV 89 + +GCHI +FKSLSP+EL +FK+A+DALEESL LK+ C S +FP WDLR LQVRERPV Sbjct: 2 SARGCHIAQFKSLSPRELQAFKRAKDALEESLLLKDCKCRSRLFPRTWDLRQLQVRERPV 61 Query: 90 ALEAELALTLKVLEAAAG--PALEDVLDQPLHTLHHILSQLQACIQPQPTAGPRPRGRLH 147 ALEAELALTLKVL+A A PAL DVLDQPLHTLHHILSQL+ACIQPQPTAGPR RGRLH Sbjct: 62 ALEAELALTLKVLDATADTDPALGDVLDQPLHTLHHILSQLRACIQPQPTAGPRTRGRLH 121 Query: 148 HWLHRLQEAPKKESAGCLEASVTFNLFRLLTRDLKYVADGNLC 190 WLHRLQEAPKKES GCLEASVTFNLFRLL RDL VA G+LC Sbjct: 122 RWLHRLQEAPKKESPGCLEASVTFNLFRLLARDLNCVASGDLC 164 >4ZN6-A nrPDB Length = 406 Score = 30.0 bits (66), Expect = 0.64, Method: Composition-based stats. Identities = 35/142 (24%), Positives = 61/142 (42%), Gaps = 17/142 (11%) Query: 3 AAWTVVLVTLVLGLAVAGPVPTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLK 62 A+ T V + + + AG +PT GK + ++L + ARD ++L Sbjct: 98 ASHTDVDIVMAAIVGAAGLLPTLAAVKAGKRVLLANKEALVMSGEIMMQAARD--HQALL 155 Query: 63 LKNWSCSSPVF---PGNWDLRLLQVRERPVALEAELALTLKVLEAAAGPALEDVLDQPLH 119 L S + +F P N+ LQ + +L ++ +L A+ GP L L+Q +H Sbjct: 156 LPVDSEHNAIFQSLPHNY----LQADRTG---QPQLGVSKILLTASGGPFLNHSLEQLVH 208 Query: 120 TLHHILSQLQACIQPQPTAGPR 141 ++ QAC P + G + Sbjct: 209 -----VTPQQACKHPNWSMGQK 225 >6EML-S nrPDB Length = 261 Score = 28.8 bits (63), Expect = 1.6, Method: Composition-based stats. Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 10/35 (28%) Query: 122 HHILSQLQACIQPQPTAGPRPRGRLHHWLHRLQEA 156 H +L +L C P+P+AGP H+L+E+ Sbjct: 17 HWLLDKLSGCYAPRPSAGP----------HKLRES 41 >3JAM-E nrPDB Length = 261 Score = 28.5 bits (62), Expect = 1.8, Method: Composition-based stats. Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 10/35 (28%) Query: 122 HHILSQLQACIQPQPTAGPRPRGRLHHWLHRLQEA 156 H +L +L C P+P+AGP H+L+E+ Sbjct: 17 HWMLDKLSGCYAPRPSAGP----------HKLRES 41 >5ZHY-A nrPDB Length = 148 Score = 27.7 bits (60), Expect = 3.4, Method: Composition-based stats. Identities = 12/33 (36%), Positives = 21/33 (63%) Query: 104 AAAGPALEDVLDQPLHTLHHILSQLQACIQPQP 136 A A ++DV++Q ++L+H+ SQL+ Q P Sbjct: 39 ATALNKIQDVVNQQGNSLNHLTSQLRQNFQLVP 71 >2CST-A nrPDB Length = 411 Score = 26.9 bits (58), Expect = 5.9, Method: Composition-based stats. Identities = 14/61 (22%), Positives = 22/61 (36%), Gaps = 4/61 (6%) Query: 22 VPTSKPTTTGKGCHIGRFKSLSPQELASFKKARDALEESLKLKNWSCSSPV----FPGNW 77 V T+ +G I SPQ A +K + + + L S + PG W Sbjct: 290 VRTTWSNPPSQGARIVATTLTSPQLFAEWKDNVKTMADRVLLMRSELRSRLESLGTPGTW 349 Query: 78 D 78 + Sbjct: 350 N 350 >5YL9-A nrPDB Length = 89 Score = 26.9 bits (58), Expect = 6.8, Method: Composition-based stats. Identities = 11/30 (36%), Positives = 20/30 (66%) Query: 104 AAAGPALEDVLDQPLHTLHHILSQLQACIQ 133 A A ++DV++Q ++L+H+ SQL+ Q Sbjct: 44 ATALNKIQDVVNQQGNSLNHLTSQLRQNFQ 73 >2X19-B nrPDB Length = 963 Score = 26.1 bits (56), Expect = 9.2, Method: Composition-based stats. Identities = 16/47 (34%), Positives = 21/47 (44%) Query: 134 PQPTAGPRPRGRLHHWLHRLQEAPKKESAGCLEASVTFNLFRLLTRD 180 PQ +A R +H + H P E+ L SVT LF+ RD Sbjct: 739 PQASALDLTRQLVHIFAHEPAHFPPIEALFLLVTSVTLTLFQQGPRD 785 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.314 0.138 0.421 Lambda K H 0.267 0.0423 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,821,646 Number of Sequences: 36641 Number of extensions: 357263 Number of successful extensions: 840 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 16 Number of HSP's successfully gapped in prelim test: 2 Number of HSP's that attempted gapping in prelim test: 819 Number of HSP's gapped (non-prelim): 18 length of query: 200 length of database: 10,336,785 effective HSP length: 92 effective length of query: 108 effective length of database: 6,965,813 effective search space: 752307804 effective search space used: 752307804 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.4 bits) S2: 57 (26.5 bits)