BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q8GS60 (462 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5DQR-A nrPDB 914 0.0 4CI0-C nrPDB 55 8e-08 4YKF-A nrPDB 31 1.3 1D3Y-A nrPDB 31 1.4 5AWF-A nrPDB 30 3.2 >5DQR-A nrPDB Length = 437 Score = 914 bits (2361), Expect = 0.0, Method: Compositional matrix adjust. Identities = 437/437 (100%), Positives = 437/437 (100%) Query: 26 SKDMNLEPKKKVKLREDWREKSRPIPPGGTYPAKDHCSQCGLCDTYYIAHVKEACAFLGD 85 SKDMNLEPKKKVKLREDWREKSRPIPPGGTYPAKDHCSQCGLCDTYYIAHVKEACAFLGD Sbjct: 1 SKDMNLEPKKKVKLREDWREKSRPIPPGGTYPAKDHCSQCGLCDTYYIAHVKEACAFLGD 60 Query: 86 GMSRIESLEPVVHGRGRKADSLQDTYFGVHQEQLYARKLKPVEGAQWTGIVTTIAIEMLK 145 GMSRIESLEPVVHGRGRKADSLQDTYFGVHQEQLYARKLKPVEGAQWTGIVTTIAIEMLK Sbjct: 61 GMSRIESLEPVVHGRGRKADSLQDTYFGVHQEQLYARKLKPVEGAQWTGIVTTIAIEMLK 120 Query: 146 SNMVEAVVCVQSDPEDRLSPRPVLARTPEEVLAARGVKPTLSPNLNTLELIEASGVKRLL 205 SNMVEAVVCVQSDPEDRLSPRPVLARTPEEVLAARGVKPTLSPNLNTLELIEASGVKRLL Sbjct: 121 SNMVEAVVCVQSDPEDRLSPRPVLARTPEEVLAARGVKPTLSPNLNTLELIEASGVKRLL 180 Query: 206 FCGVGCQVQALRSVEQHLNLEKLYVLGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQ 265 FCGVGCQVQALRSVEQHLNLEKLYVLGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQ Sbjct: 181 FCGVGCQVQALRSVEQHLNLEKLYVLGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQ 240 Query: 266 DYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSCFDYTNALADLVIGYMGVPKYSGL 325 DYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSCFDYTNALADLVIGYMGVPKYSGL Sbjct: 241 DYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSCFDYTNALADLVIGYMGVPKYSGL 300 Query: 326 NMTDHPQYITVRNERGKEMLSLVENLLEITPTISSGDRRPFVTETVKADDAAKFGQGPAQ 385 NMTDHPQYITVRNERGKEMLSLVENLLEITPTISSGDRRPFVTETVKADDAAKFGQGPAQ Sbjct: 301 NMTDHPQYITVRNERGKEMLSLVENLLEITPTISSGDRRPFVTETVKADDAAKFGQGPAQ 360 Query: 386 PAPLFVGNIIAFILNLVGPKGLEFARYSLDYHTIRNYLYVNRKWGKQRANTHMPSYAKKI 445 PAPLFVGNIIAFILNLVGPKGLEFARYSLDYHTIRNYLYVNRKWGKQRANTHMPSYAKKI Sbjct: 361 PAPLFVGNIIAFILNLVGPKGLEFARYSLDYHTIRNYLYVNRKWGKQRANTHMPSYAKKI 420 Query: 446 VEMYNKNGQIDKMLSKK 462 VEMYNKNGQIDKMLSKK Sbjct: 421 VEMYNKNGQIDKMLSKK 437 >4CI0-C nrPDB Length = 281 Score = 54.7 bits (130), Expect = 8e-08, Method: Compositional matrix adjust. Identities = 49/198 (24%), Positives = 88/198 (44%), Gaps = 14/198 (7%) Query: 130 AQWTGIVTTIAIEMLKSNMVEAVVCVQSDPEDRLSPRPVLARTPEEVLAARGVKPTLSPN 189 AQ GIVT + L ++E V V E+ P+P++A + +E+ AA G K T SPN Sbjct: 23 AQDGGIVTGLLAYALDEGIIEGAV-VAGPGEEFWKPQPMVAMSSDELKAAAGTKYTFSPN 81 Query: 190 LNTL-ELIEASGVKRLLFCGVGCQVQALRSVEQH-----LNLEKLYVL-GTNCVDNGTRD 242 + L + + G+++L + CQ +R ++ + +K+ +L G C++N Sbjct: 82 VMMLKKAVRQYGIEKLGTVAIPCQTMGIRKMQTYPFGVRFLADKIKLLVGIYCMENFPYT 141 Query: 243 GLDKFLKAASKEPETVLHYEFMQDYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSC 302 L F+ + + E ++ + + ++ +LP + C C Sbjct: 142 SLQTFIC-----EKLGVSMELVEKMDIGKGKFWVYTQD-DVLTLPLKETHGYEQAGCKIC 195 Query: 303 FDYTNALADLVIGYMGVP 320 DY LAD+ G +G P Sbjct: 196 KDYVAELADVSTGSVGSP 213 >4YKF-A nrPDB Length = 521 Score = 30.8 bits (68), Expect = 1.3, Method: Compositional matrix adjust. Identities = 35/131 (26%), Positives = 52/131 (39%), Gaps = 29/131 (22%) Query: 252 SKEPETVLHYEFMQDYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSCFDYTNALAD 311 SKE +++L Q++H+DG E Y+SL SC++C D AL + Sbjct: 102 SKEAQSLLE---------QIRHIDGDFEFETYYSL-----------SCHNCPDVVQAL-N 140 Query: 312 LVIGYMGVPKYSGLNMTDHPQYITVRN--------ERGKEMLSLVENLLEITPTISSGDR 363 L+ K++ ++ IT RN GKE L EI I +G Sbjct: 141 LMSVLNPRIKHTAIDGGTFQNEITDRNVMGVPAVFVNGKEFGQGRMTLTEIVAKIDTGAE 200 Query: 364 RPFVTETVKAD 374 + E K D Sbjct: 201 KRAAEELNKRD 211 >1D3Y-A nrPDB Length = 301 Score = 30.8 bits (68), Expect = 1.4, Method: Compositional matrix adjust. Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 3/39 (7%) Query: 405 KGLEFARYSL---DYHTIRNYLYVNRKWGKQRANTHMPS 440 K LEFA+ L D+ T+R YV++ WG+ R + S Sbjct: 13 KMLEFAKQLLETDDFSTLREAYYVSKNWGEARFDDQQAS 51 >5AWF-A nrPDB Length = 495 Score = 29.6 bits (65), Expect = 3.2, Method: Compositional matrix adjust. Identities = 20/74 (27%), Positives = 34/74 (45%), Gaps = 1/74 (1%) Query: 231 LGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQDYKVQLKHLDGHIEEVPYFSLPAND 290 L T+ + G + + + + A EPE +L + + Y+ L+ + H + Y L D Sbjct: 29 LATDELAKGINEEVVRAISAKRNEPEWMLEFR-LNAYRAWLEMEEPHWLKAHYDKLNYQD 87 Query: 291 LVDVIAPSCYSCFD 304 APSC +C D Sbjct: 88 YSYYSAPSCGNCDD 101 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5DQR-A nrPDB 860 0.0 4CI0-C nrPDB 282 2e-76 Sequences not found previously or not previously below threshold: 1D3Y-A nrPDB 31 1.5 4LS7-A nrPDB 30 1.9 2OT8-A nrPDB 29 3.3 CONVERGED! >5DQR-A nrPDB Length = 437 Score = 860 bits (2223), Expect = 0.0, Method: Composition-based stats. Identities = 437/437 (100%), Positives = 437/437 (100%) Query: 26 SKDMNLEPKKKVKLREDWREKSRPIPPGGTYPAKDHCSQCGLCDTYYIAHVKEACAFLGD 85 SKDMNLEPKKKVKLREDWREKSRPIPPGGTYPAKDHCSQCGLCDTYYIAHVKEACAFLGD Sbjct: 1 SKDMNLEPKKKVKLREDWREKSRPIPPGGTYPAKDHCSQCGLCDTYYIAHVKEACAFLGD 60 Query: 86 GMSRIESLEPVVHGRGRKADSLQDTYFGVHQEQLYARKLKPVEGAQWTGIVTTIAIEMLK 145 GMSRIESLEPVVHGRGRKADSLQDTYFGVHQEQLYARKLKPVEGAQWTGIVTTIAIEMLK Sbjct: 61 GMSRIESLEPVVHGRGRKADSLQDTYFGVHQEQLYARKLKPVEGAQWTGIVTTIAIEMLK 120 Query: 146 SNMVEAVVCVQSDPEDRLSPRPVLARTPEEVLAARGVKPTLSPNLNTLELIEASGVKRLL 205 SNMVEAVVCVQSDPEDRLSPRPVLARTPEEVLAARGVKPTLSPNLNTLELIEASGVKRLL Sbjct: 121 SNMVEAVVCVQSDPEDRLSPRPVLARTPEEVLAARGVKPTLSPNLNTLELIEASGVKRLL 180 Query: 206 FCGVGCQVQALRSVEQHLNLEKLYVLGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQ 265 FCGVGCQVQALRSVEQHLNLEKLYVLGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQ Sbjct: 181 FCGVGCQVQALRSVEQHLNLEKLYVLGTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQ 240 Query: 266 DYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSCFDYTNALADLVIGYMGVPKYSGL 325 DYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSCFDYTNALADLVIGYMGVPKYSGL Sbjct: 241 DYKVQLKHLDGHIEEVPYFSLPANDLVDVIAPSCYSCFDYTNALADLVIGYMGVPKYSGL 300 Query: 326 NMTDHPQYITVRNERGKEMLSLVENLLEITPTISSGDRRPFVTETVKADDAAKFGQGPAQ 385 NMTDHPQYITVRNERGKEMLSLVENLLEITPTISSGDRRPFVTETVKADDAAKFGQGPAQ Sbjct: 301 NMTDHPQYITVRNERGKEMLSLVENLLEITPTISSGDRRPFVTETVKADDAAKFGQGPAQ 360 Query: 386 PAPLFVGNIIAFILNLVGPKGLEFARYSLDYHTIRNYLYVNRKWGKQRANTHMPSYAKKI 445 PAPLFVGNIIAFILNLVGPKGLEFARYSLDYHTIRNYLYVNRKWGKQRANTHMPSYAKKI Sbjct: 361 PAPLFVGNIIAFILNLVGPKGLEFARYSLDYHTIRNYLYVNRKWGKQRANTHMPSYAKKI 420 Query: 446 VEMYNKNGQIDKMLSKK 462 VEMYNKNGQIDKMLSKK Sbjct: 421 VEMYNKNGQIDKMLSKK 437 >4CI0-C nrPDB Length = 281 Score = 282 bits (721), Expect = 2e-76, Method: Composition-based stats. Identities = 53/218 (24%), Positives = 98/218 (44%), Gaps = 16/218 (7%) Query: 112 FGVHQEQLYARKL-KPVEG-AQWTGIVTTIAIEMLKSNMVEAVVCVQSDPEDRLSPRPVL 169 G ++E + AR + ++ AQ GIVT + L ++E V V E+ P+P++ Sbjct: 3 LGTYKEIVSARSTDREIQKLAQDGGIVTGLLAYALDEGIIEGAV-VAGPGEEFWKPQPMV 61 Query: 170 ARTPEEVLAARGVKPTLSPNLNTL-ELIEASGVKRLLFCGVGCQVQALRSVEQH-----L 223 A + +E+ AA G K T SPN+ L + + G+++L + CQ +R ++ + Sbjct: 62 AMSSDELKAAAGTKYTFSPNVMMLKKAVRQYGIEKLGTVAIPCQTMGIRKMQTYPFGVRF 121 Query: 224 NLEKLYVL-GTNCVDNGTRDGLDKFLKAASKEPETVLHYEFMQDYKVQLKHLDGHIEEVP 282 +K+ +L G C++N L F+ + + E ++ + + ++ Sbjct: 122 LADKIKLLVGIYCMENFPYTSLQTFIC-----EKLGVSMELVEKMDIGKGKFWVYTQD-D 175 Query: 283 YFSLPANDLVDVIAPSCYSCFDYTNALADLVIGYMGVP 320 +LP + C C DY LAD+ G +G P Sbjct: 176 VLTLPLKETHGYEQAGCKICKDYVAELADVSTGSVGSP 213 >1D3Y-A nrPDB Length = 301 Score = 30.8 bits (68), Expect = 1.5, Method: Composition-based stats. Identities = 15/39 (38%), Positives = 22/39 (56%), Gaps = 3/39 (7%) Query: 405 KGLEFARYSL---DYHTIRNYLYVNRKWGKQRANTHMPS 440 K LEFA+ L D+ T+R YV++ WG+ R + S Sbjct: 13 KMLEFAKQLLETDDFSTLREAYYVSKNWGEARFDDQQAS 51 >4LS7-A nrPDB Length = 426 Score = 30.4 bits (67), Expect = 1.9, Method: Composition-based stats. Identities = 13/55 (23%), Positives = 23/55 (41%), Gaps = 3/55 (5%) Query: 110 TYFGVHQEQLYARKLKPVEG---AQWTGIVTTIAIEMLKSNMVEAVVCVQSDPED 161 T FG H +L K + G GI +I +K ++ + +Q+ E+ Sbjct: 333 TVFGEHAHKLAVSSTKSMTGHLLGAAGGIEAIFSILAIKEGVIPPTINIQTPDEE 387 >2OT8-A nrPDB Length = 852 Score = 29.3 bits (64), Expect = 3.3, Method: Composition-based stats. Identities = 21/88 (23%), Positives = 36/88 (40%), Gaps = 4/88 (4%) Query: 278 IEEVPYFSLPANDLV----DVIAPSCYSCFDYTNALADLVIGYMGVPKYSGLNMTDHPQY 333 E VPY + + LV + +D LAD V ++ P+Y + M Q Sbjct: 475 TELVPYLAYILDTLVFAFSKYQHKNLLILYDAIGTLADSVGHHLNKPEYIQMLMPPLIQK 534 Query: 334 ITVRNERGKEMLSLVENLLEITPTISSG 361 + + K++ L+E L + + SG Sbjct: 535 WNMLKDEDKDLFPLLECLSSVATALQSG 562 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.134 0.380 Lambda K H 0.267 0.0414 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 18,061,489 Number of Sequences: 36641 Number of extensions: 756879 Number of successful extensions: 1798 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 1788 Number of HSP's gapped (non-prelim): 13 length of query: 462 length of database: 10,336,785 effective HSP length: 100 effective length of query: 362 effective length of database: 6,672,685 effective search space: 2415511970 effective search space used: 2415511970 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 61 (28.1 bits)