BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= Q8GCH2
         (400 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

2HY7-A nrPDB                                                          819   0.0  
2DGM-A nrPDB                                                           31   0.96 
4PEL-B nrPDB                                                           28   5.8  

>2HY7-A nrPDB
          Length = 406

 Score =  819 bits (2116), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 397/400 (99%), Positives = 398/400 (99%)

Query: 1   MSVSPAAPASGIRRPCYLVLSAHDFRTPRRANIHFITDQLALRGTTRFFSLRYSRLSRMK 60
           M VSPAAPASGIRRPCYLVLS+HDFRTPRRANIHFITDQLALRGTTRFFSLRYSRLSRMK
Sbjct: 1   MGVSPAAPASGIRRPCYLVLSSHDFRTPRRANIHFITDQLALRGTTRFFSLRYSRLSRMK 60

Query: 61  GDMRLPLDDTANTVVSHNGVDCYLWRTTVHPFNTRRSWLRPVEDAMFRWYAAHPPKQLLD 120
           GDMRLPLDDTANTVVSHNGVDCYLWRTTVHPFNTRRSWLRPVEDAMFRWYAAHPPKQLLD
Sbjct: 61  GDMRLPLDDTANTVVSHNGVDCYLWRTTVHPFNTRRSWLRPVEDAMFRWYAAHPPKQLLD 120

Query: 121 WMRESDVIVFESGIAVAFIELAKRVNPAAKLVYRASDGLSTINVASYIEREFDRVAPTLD 180
           WMRESDVIVFESGIAVAFIELAKRVNPAAKLVYRASDGLSTINVASYIEREFDRVAPTLD
Sbjct: 121 WMRESDVIVFESGIAVAFIELAKRVNPAAKLVYRASDGLSTINVASYIEREFDRVAPTLD 180

Query: 181 VIALVSPAMAAEVASRDNVFHVGHGVDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVV 240
           VIALVSPAMAAEV SRDNVFHVGHGVDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVV
Sbjct: 181 VIALVSPAMAAEVVSRDNVFHVGHGVDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVV 240

Query: 241 ASKAFPQVTFHVIGSGMGRHPGYGDNVIVYGEMKHAQTIGYIKHARFGIAPYASEQVPVY 300
           ASKAFPQVTFHVIGSGMGRHPGYGDNVIVYGEMKHAQTIGYIKHARFGIAPYASEQVPVY
Sbjct: 241 ASKAFPQVTFHVIGSGMGRHPGYGDNVIVYGEMKHAQTIGYIKHARFGIAPYASEQVPVY 300

Query: 301 LADSSMKLLQYDFFGLPAVCPNAVVGPYKSRFGYTPGNADSVIAAITQALEAPRVRYRQC 360
           LADSSMKLLQYDFFGLPAVCPNAVVGPYKSRFGYTPGNADSVIAAITQALEAPRVRYRQC
Sbjct: 301 LADSSMKLLQYDFFGLPAVCPNAVVGPYKSRFGYTPGNADSVIAAITQALEAPRVRYRQC 360

Query: 361 LNWSDTTDRVLDPRAYPETRLYPHPPTAAPQLSSEAALSH 400
           LNWSDTTDRVLDPRAYPETRLYPHPPTAAPQLSSEAALSH
Sbjct: 361 LNWSDTTDRVLDPRAYPETRLYPHPPTAAPQLSSEAALSH 400


>2DGM-A nrPDB
          Length = 466

 Score = 30.8 bits (68), Expect = 0.96,   Method: Compositional matrix adjust.
 Identities = 25/71 (35%), Positives = 32/71 (45%), Gaps = 16/71 (22%)

Query: 276 AQTIGYIKHARFGIAPY--ASEQVPVYLADSSMKLLQYDFF-------GLPAVCPNAVVG 326
           AQ   +++  R G      AS QV  YLAD   KL  Y+F        G+PAVC      
Sbjct: 326 AQYYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVC------ 379

Query: 327 PYKSRFGYTPG 337
            +K + G  PG
Sbjct: 380 -FKLKDGEDPG 389


>4PEL-B nrPDB
          Length = 565

 Score = 28.5 bits (62), Expect = 5.8,   Method: Compositional matrix adjust.
 Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 1/42 (2%)

Query: 244 AFPQVTFHVIGS-GMGRHPGYGDNVIVYGEMKHAQTIGYIKH 284
           A+P + F   G+   G   G+GD+V ++ E   A+  GY +H
Sbjct: 51  AYPGLVFGHNGTISWGSTAGFGDDVDIFAEKLSAEKPGYYQH 92


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

2HY7-A nrPDB                                                          818   0.0  

Sequences not found previously or not previously below threshold:

2DGM-A nrPDB                                                           32   0.38 
5FV0-A nrPDB                                                           30   1.9  
2OQ5-A nrPDB                                                           29   3.7  
4PEL-B nrPDB                                                           28   6.9  
2DF5-A nrPDB                                                           28   7.1  


CONVERGED!
>2HY7-A nrPDB
          Length = 406

 Score =  818 bits (2114), Expect = 0.0,   Method: Composition-based stats.
 Identities = 397/400 (99%), Positives = 398/400 (99%)

Query: 1   MSVSPAAPASGIRRPCYLVLSAHDFRTPRRANIHFITDQLALRGTTRFFSLRYSRLSRMK 60
           M VSPAAPASGIRRPCYLVLS+HDFRTPRRANIHFITDQLALRGTTRFFSLRYSRLSRMK
Sbjct: 1   MGVSPAAPASGIRRPCYLVLSSHDFRTPRRANIHFITDQLALRGTTRFFSLRYSRLSRMK 60

Query: 61  GDMRLPLDDTANTVVSHNGVDCYLWRTTVHPFNTRRSWLRPVEDAMFRWYAAHPPKQLLD 120
           GDMRLPLDDTANTVVSHNGVDCYLWRTTVHPFNTRRSWLRPVEDAMFRWYAAHPPKQLLD
Sbjct: 61  GDMRLPLDDTANTVVSHNGVDCYLWRTTVHPFNTRRSWLRPVEDAMFRWYAAHPPKQLLD 120

Query: 121 WMRESDVIVFESGIAVAFIELAKRVNPAAKLVYRASDGLSTINVASYIEREFDRVAPTLD 180
           WMRESDVIVFESGIAVAFIELAKRVNPAAKLVYRASDGLSTINVASYIEREFDRVAPTLD
Sbjct: 121 WMRESDVIVFESGIAVAFIELAKRVNPAAKLVYRASDGLSTINVASYIEREFDRVAPTLD 180

Query: 181 VIALVSPAMAAEVASRDNVFHVGHGVDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVV 240
           VIALVSPAMAAEV SRDNVFHVGHGVDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVV
Sbjct: 181 VIALVSPAMAAEVVSRDNVFHVGHGVDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVV 240

Query: 241 ASKAFPQVTFHVIGSGMGRHPGYGDNVIVYGEMKHAQTIGYIKHARFGIAPYASEQVPVY 300
           ASKAFPQVTFHVIGSGMGRHPGYGDNVIVYGEMKHAQTIGYIKHARFGIAPYASEQVPVY
Sbjct: 241 ASKAFPQVTFHVIGSGMGRHPGYGDNVIVYGEMKHAQTIGYIKHARFGIAPYASEQVPVY 300

Query: 301 LADSSMKLLQYDFFGLPAVCPNAVVGPYKSRFGYTPGNADSVIAAITQALEAPRVRYRQC 360
           LADSSMKLLQYDFFGLPAVCPNAVVGPYKSRFGYTPGNADSVIAAITQALEAPRVRYRQC
Sbjct: 301 LADSSMKLLQYDFFGLPAVCPNAVVGPYKSRFGYTPGNADSVIAAITQALEAPRVRYRQC 360

Query: 361 LNWSDTTDRVLDPRAYPETRLYPHPPTAAPQLSSEAALSH 400
           LNWSDTTDRVLDPRAYPETRLYPHPPTAAPQLSSEAALSH
Sbjct: 361 LNWSDTTDRVLDPRAYPETRLYPHPPTAAPQLSSEAALSH 400


>2DGM-A nrPDB
          Length = 466

 Score = 32.3 bits (72), Expect = 0.38,   Method: Composition-based stats.
 Identities = 25/69 (36%), Positives = 30/69 (43%), Gaps = 19/69 (27%)

Query: 281 YIKHARFGIAPY-----ASEQVPVYLADSSMKLLQYDFF-------GLPAVCPNAVVGPY 328
           Y +  R G   Y     AS QV  YLAD   KL  Y+F        G+PAVC       +
Sbjct: 328 YYEFLRLGREGYTKVQNASYQVAAYLADEIAKLGPYEFICTGRPDEGIPAVC-------F 380

Query: 329 KSRFGYTPG 337
           K + G  PG
Sbjct: 381 KLKDGEDPG 389


>5FV0-A nrPDB
          Length = 517

 Score = 30.0 bits (66), Expect = 1.9,   Method: Composition-based stats.
 Identities = 40/180 (22%), Positives = 74/180 (41%), Gaps = 34/180 (18%)

Query: 141 LAKRVNPAAKLVYRASDGLSTINVASYIEREFDRVA------PTLDVIALVSPAMAAEVA 194
           L K+ N A   +Y  +DG   I V   ++RE +R+       P    I ++ P ++  V 
Sbjct: 224 LIKKDNAALTQIYLPADGEDDIEVLENVKREMERLKEVYQHIPKPKPIPMLPPRLSMSVF 283

Query: 195 SRDNVFHVGHG-VDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVV--ASKAFPQVTFH 251
           +   V H   G +   LD+        + +  VA+ +M  DP   +V  + K    V   
Sbjct: 284 TNTYVQHRASGFIPVGLDE--------QTVRPVAI-NMRTDPHCLIVGQSRKGKTNVVKV 334

Query: 252 VIGSGMGRHPGYGDNVIVYGEMKHAQTIGYIKHARFGIAPYASEQVPVYLADSSMKLLQY 311
           ++ S + + P               ++IG +     G+A YA+     Y+ ++  +L Q+
Sbjct: 335 ILESLLVQEP---------------ESIGLLDGIDRGLAGYANRDDITYI-EAKERLAQW 378


>2OQ5-A nrPDB
          Length = 232

 Score = 29.2 bits (64), Expect = 3.7,   Method: Composition-based stats.
 Identities = 21/67 (31%), Positives = 28/67 (41%), Gaps = 11/67 (16%)

Query: 201 HVGHGVDHNLDQLGDPSPYAEGIHAVAVGSMLFDPEFFVVASKAF-PQVTFHVIGSGMGR 259
           H  H  D +L +L  P PY   +H V +      P+    AS  F P     V G G  +
Sbjct: 80  HPSHDYDISLAELSSPVPYTNAVHRVCL------PD----ASYEFQPGDVMFVTGFGALK 129

Query: 260 HPGYGDN 266
           + GY  N
Sbjct: 130 NDGYSQN 136


>4PEL-B nrPDB
          Length = 565

 Score = 28.0 bits (61), Expect = 6.9,   Method: Composition-based stats.
 Identities = 14/42 (33%), Positives = 23/42 (54%), Gaps = 1/42 (2%)

Query: 244 AFPQVTFHVIGS-GMGRHPGYGDNVIVYGEMKHAQTIGYIKH 284
           A+P + F   G+   G   G+GD+V ++ E   A+  GY +H
Sbjct: 51  AYPGLVFGHNGTISWGSTAGFGDDVDIFAEKLSAEKPGYYQH 92


>2DF5-A nrPDB
          Length = 213

 Score = 28.0 bits (61), Expect = 7.1,   Method: Composition-based stats.
 Identities = 12/32 (37%), Positives = 18/32 (56%)

Query: 266 NVIVYGEMKHAQTIGYIKHARFGIAPYASEQV 297
           N ++Y  + H+ T GY K + F   PY  EQ+
Sbjct: 143 NYVMYLSLHHSATKGYPKMSGFIHVPYIPEQI 174


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.314    0.141    0.461 

Lambda     K      H
   0.267   0.0417    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 16,943,158
Number of Sequences: 36641
Number of extensions: 713122
Number of successful extensions: 1367
Number of sequences better than 10.0: 6
Number of HSP's better than 10.0 without gapping: 2
Number of HSP's successfully gapped in prelim test: 10
Number of HSP's that attempted gapping in prelim test: 1365
Number of HSP's gapped (non-prelim): 12
length of query: 400
length of database: 10,336,785
effective HSP length: 99
effective length of query: 301
effective length of database: 6,709,326
effective search space: 2019507126
effective search space used: 2019507126
T: 11
A: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.4 bits)
S2: 60 (27.7 bits)