BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q86WV5 (123 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4JOI-D nrPDB 251 5e-68 4XIG-S nrPDB 27 3.0 3QUB-A nrPDB 26 3.4 1O92-A nrPDB 26 4.7 2YIB-D nrPDB 25 8.0 2YIB-A nrPDB 25 8.6 >4JOI-D nrPDB Length = 122 Score = 251 bits (641), Expect = 5e-68, Method: Compositional matrix adjust. Identities = 122/122 (100%), Positives = 122/122 (100%) Query: 2 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL 61 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL Sbjct: 1 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL 60 Query: 62 VEPFHAQVGSLYIVLGELQHQQDRGSVVKARVLTCVEGMNLPLLEQAIREQRLYKQERGG 121 VEPFHAQVGSLYIVLGELQHQQDRGSVVKARVLTCVEGMNLPLLEQAIREQRLYKQERGG Sbjct: 61 VEPFHAQVGSLYIVLGELQHQQDRGSVVKARVLTCVEGMNLPLLEQAIREQRLYKQERGG 120 Query: 122 SQ 123 SQ Sbjct: 121 SQ 122 >4XIG-S nrPDB Length = 363 Score = 26.6 bits (57), Expect = 3.0, Method: Compositional matrix adjust. Identities = 23/87 (26%), Positives = 36/87 (41%), Gaps = 25/87 (28%) Query: 2 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL 61 +LP+ GT+ P G+ ++ + VTL GS++ Q+ + Sbjct: 265 LLPRAGTHLAP-----------------GQEVVFGIRPEDVTLDGVEGSERAQIKATVDI 307 Query: 62 VEP------FHAQVG--SLYIVLGELQ 80 VEP HA VG SL + +G L Sbjct: 308 VEPLGSESILHATVGDHSLVVKVGGLN 334 >3QUB-A nrPDB Length = 243 Score = 26.2 bits (56), Expect = 3.4, Method: Compositional matrix adjust. Identities = 14/39 (35%), Positives = 18/39 (46%) Query: 18 GQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVL 56 G VP GS +R + L+DM M H HQV+ Sbjct: 12 GLVPRGSHMRKKLKAVLFDMDGVLFNSMPYHSEAWHQVM 50 >1O92-A nrPDB Length = 396 Score = 25.8 bits (55), Expect = 4.7, Method: Compositional matrix adjust. Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 6/40 (15%) Query: 79 LQHQQDRGSVVKARVLTCV------EGMNLPLLEQAIREQ 112 +Q+ QD G+V+ RV T V E + L + +A++EQ Sbjct: 187 VQYVQDNGAVIPVRVHTIVISVQHNEDITLEAMREALKEQ 226 >2YIB-D nrPDB Length = 770 Score = 25.0 bits (53), Expect = 8.0, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 18/32 (56%) Query: 88 VVKARVLTCVEGMNLPLLEQAIREQRLYKQER 119 +V+ + V G N PLLE A + L K++R Sbjct: 610 IVRYEAIRLVGGWNNPLLETAAKHMSLDKRKR 641 >2YIB-A nrPDB Length = 853 Score = 25.0 bits (53), Expect = 8.6, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 18/32 (56%) Query: 88 VVKARVLTCVEGMNLPLLEQAIREQRLYKQER 119 +V+ + V G N PLLE A + L K++R Sbjct: 610 IVRYEAIRLVGGWNNPLLETAAKHMSLDKRKR 641 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4JOI-D nrPDB 250 1e-67 Sequences not found previously or not previously below threshold: 1O92-A nrPDB 27 2.6 3QUB-A nrPDB 26 4.0 4XIG-S nrPDB 26 4.1 2YIB-D nrPDB 25 9.2 2YIB-A nrPDB 25 9.2 CONVERGED! >4JOI-D nrPDB Length = 122 Score = 250 bits (639), Expect = 1e-67, Method: Composition-based stats. Identities = 122/122 (100%), Positives = 122/122 (100%) Query: 2 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL 61 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL Sbjct: 1 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL 60 Query: 62 VEPFHAQVGSLYIVLGELQHQQDRGSVVKARVLTCVEGMNLPLLEQAIREQRLYKQERGG 121 VEPFHAQVGSLYIVLGELQHQQDRGSVVKARVLTCVEGMNLPLLEQAIREQRLYKQERGG Sbjct: 61 VEPFHAQVGSLYIVLGELQHQQDRGSVVKARVLTCVEGMNLPLLEQAIREQRLYKQERGG 120 Query: 122 SQ 123 SQ Sbjct: 121 SQ 122 >1O92-A nrPDB Length = 396 Score = 26.5 bits (57), Expect = 2.6, Method: Composition-based stats. Identities = 14/40 (35%), Positives = 23/40 (57%), Gaps = 6/40 (15%) Query: 79 LQHQQDRGSVVKARVLTCV------EGMNLPLLEQAIREQ 112 +Q+ QD G+V+ RV T V E + L + +A++EQ Sbjct: 187 VQYVQDNGAVIPVRVHTIVISVQHNEDITLEAMREALKEQ 226 >3QUB-A nrPDB Length = 243 Score = 26.2 bits (56), Expect = 4.0, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 18/39 (46%) Query: 18 GQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVL 56 G VP GS +R + L+DM M H HQV+ Sbjct: 12 GLVPRGSHMRKKLKAVLFDMDGVLFNSMPYHSEAWHQVM 50 >4XIG-S nrPDB Length = 363 Score = 26.2 bits (56), Expect = 4.1, Method: Composition-based stats. Identities = 23/86 (26%), Positives = 36/86 (41%), Gaps = 25/86 (29%) Query: 2 MLPKPGTYYLPWEVSAGQVPDGSTLRTFGRLCLYDMIQSRVTLMAQHGSDQHQVLVCTKL 61 +LP+ GT+ P G+ ++ + VTL GS++ Q+ + Sbjct: 265 LLPRAGTHLAP-----------------GQEVVFGIRPEDVTLDGVEGSERAQIKATVDI 307 Query: 62 VEP------FHAQVG--SLYIVLGEL 79 VEP HA VG SL + +G L Sbjct: 308 VEPLGSESILHATVGDHSLVVKVGGL 333 >2YIB-D nrPDB Length = 770 Score = 25.0 bits (53), Expect = 9.2, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 18/32 (56%) Query: 88 VVKARVLTCVEGMNLPLLEQAIREQRLYKQER 119 +V+ + V G N PLLE A + L K++R Sbjct: 610 IVRYEAIRLVGGWNNPLLETAAKHMSLDKRKR 641 >2YIB-A nrPDB Length = 853 Score = 25.0 bits (53), Expect = 9.2, Method: Composition-based stats. Identities = 12/32 (37%), Positives = 18/32 (56%) Query: 88 VVKARVLTCVEGMNLPLLEQAIREQRLYKQER 119 +V+ + V G N PLLE A + L K++R Sbjct: 610 IVRYEAIRLVGGWNNPLLETAAKHMSLDKRKR 641 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.137 0.409 Lambda K H 0.267 0.0420 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,933,961 Number of Sequences: 36641 Number of extensions: 171788 Number of successful extensions: 324 Number of sequences better than 10.0: 6 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 4 Number of HSP's that attempted gapping in prelim test: 316 Number of HSP's gapped (non-prelim): 12 length of query: 123 length of database: 10,336,785 effective HSP length: 85 effective length of query: 38 effective length of database: 7,222,300 effective search space: 274447400 effective search space used: 274447400 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (25.0 bits)