BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q86LE5 (110 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2KNJ-A nrPDB 189 2e-49 5MLK-A nrPDB 29 0.54 1OJM-A nrPDB 27 2.4 3L44-A nrPDB 26 3.2 6DEO-A nrPDB 26 4.2 2EWO-A nrPDB 25 6.5 1MJG-A nrPDB 25 7.8 >2KNJ-A nrPDB Length = 90 Score = 189 bits (481), Expect = 2e-49, Method: Compositional matrix adjust. Identities = 90/90 (100%), Positives = 90/90 (100%) Query: 21 HHQELCTKGDDALVTELECIRLRISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQA 80 HHQELCTKGDDALVTELECIRLRISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQA Sbjct: 1 HHQELCTKGDDALVTELECIRLRISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQA 60 Query: 81 MSVYFTNEQIKEIHDAATACDPEAHHEHDH 110 MSVYFTNEQIKEIHDAATACDPEAHHEHDH Sbjct: 61 MSVYFTNEQIKEIHDAATACDPEAHHEHDH 90 >5MLK-A nrPDB Length = 614 Score = 28.9 bits (63), Expect = 0.54, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 20/30 (66%) Query: 77 LEQAMSVYFTNEQIKEIHDAATACDPEAHH 106 +E+A + + T+ Q KEIHD+A EAH+ Sbjct: 263 VEEAPAPFLTDFQRKEIHDSAKRICKEAHY 292 >1OJM-A nrPDB Length = 729 Score = 26.6 bits (57), Expect = 2.4, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 17/34 (50%) Query: 73 ATNNLEQAMSVYFTNEQIKEIHDAATACDPEAHH 106 A NN M YF++E+IK+ D P+ H Sbjct: 132 AINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPEH 165 >3L44-A nrPDB Length = 434 Score = 26.2 bits (56), Expect = 3.2, Method: Composition-based stats. Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Query: 62 LNRACAYRKMCATNNLEQAMSVYFTNEQIKEIHDAATACDPE 103 L +A + N L+ A++VYFT I++ +DAA D E Sbjct: 341 LEQAAKHNIDITLNRLKGALTVYFTTNTIED-YDAAQDTDGE 381 >6DEO-A nrPDB Length = 682 Score = 25.8 bits (55), Expect = 4.2, Method: Composition-based stats. Identities = 13/38 (34%), Positives = 22/38 (57%) Query: 44 ISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQAM 81 I + +A+F+ + +L+ +A A K+C NN EQ M Sbjct: 540 IDIDGDASFNMTLTELSSAVQAGAPIKVCVLNNEEQGM 577 >2EWO-A nrPDB Length = 377 Score = 25.4 bits (54), Expect = 6.5, Method: Composition-based stats. Identities = 14/47 (29%), Positives = 21/47 (44%), Gaps = 3/47 (6%) Query: 47 ETNAAFDNAVQQLNCLNRACAYRKMCATNN---LEQAMSVYFTNEQI 90 E AA+D QQ + R KMC T L++A ++ + I Sbjct: 250 EAKAAYDFLSQQTDAKGRPLKVHKMCVTKEPCYLQEAATIDYVEGSI 296 >1MJG-A nrPDB Length = 674 Score = 25.0 bits (53), Expect = 7.8, Method: Compositional matrix adjust. Identities = 11/34 (32%), Positives = 19/34 (55%) Query: 12 VALVASTSAHHQELCTKGDDALVTELECIRLRIS 45 + LV S ++ +CT DA+ +++CI IS Sbjct: 328 IPLVTSFASQELAICTGAIDAMCVDVQCIMPSIS 361 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2KNJ-A nrPDB 170 1e-43 Sequences not found previously or not previously below threshold: 5MLK-A nrPDB 29 0.52 1OJM-A nrPDB 27 2.6 3L44-A nrPDB 26 3.4 1MJG-A nrPDB 26 3.8 3FHG-A nrPDB 26 4.5 6DEO-A nrPDB 26 4.5 2LD5-A nrPDB 26 4.8 6FWS-A nrPDB 25 5.6 2EWO-A nrPDB 25 7.3 5OQR-A nrPDB 25 9.2 CONVERGED! >2KNJ-A nrPDB Length = 90 Score = 170 bits (431), Expect = 1e-43, Method: Composition-based stats. Identities = 90/90 (100%), Positives = 90/90 (100%) Query: 21 HHQELCTKGDDALVTELECIRLRISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQA 80 HHQELCTKGDDALVTELECIRLRISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQA Sbjct: 1 HHQELCTKGDDALVTELECIRLRISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQA 60 Query: 81 MSVYFTNEQIKEIHDAATACDPEAHHEHDH 110 MSVYFTNEQIKEIHDAATACDPEAHHEHDH Sbjct: 61 MSVYFTNEQIKEIHDAATACDPEAHHEHDH 90 >5MLK-A nrPDB Length = 614 Score = 28.9 bits (63), Expect = 0.52, Method: Composition-based stats. Identities = 13/30 (43%), Positives = 20/30 (66%) Query: 77 LEQAMSVYFTNEQIKEIHDAATACDPEAHH 106 +E+A + + T+ Q KEIHD+A EAH+ Sbjct: 263 VEEAPAPFLTDFQRKEIHDSAKRICKEAHY 292 >1OJM-A nrPDB Length = 729 Score = 26.6 bits (57), Expect = 2.6, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 17/34 (50%) Query: 73 ATNNLEQAMSVYFTNEQIKEIHDAATACDPEAHH 106 A NN M YF++E+IK+ D P+ H Sbjct: 132 AINNTLSLMKEYFSDEEIKKYTDVIEKFVPDPEH 165 >3L44-A nrPDB Length = 434 Score = 26.2 bits (56), Expect = 3.4, Method: Composition-based stats. Identities = 15/42 (35%), Positives = 23/42 (54%), Gaps = 1/42 (2%) Query: 62 LNRACAYRKMCATNNLEQAMSVYFTNEQIKEIHDAATACDPE 103 L +A + N L+ A++VYFT I++ +DAA D E Sbjct: 341 LEQAAKHNIDITLNRLKGALTVYFTTNTIED-YDAAQDTDGE 381 >1MJG-A nrPDB Length = 674 Score = 25.9 bits (55), Expect = 3.8, Method: Composition-based stats. Identities = 11/34 (32%), Positives = 19/34 (55%) Query: 12 VALVASTSAHHQELCTKGDDALVTELECIRLRIS 45 + LV S ++ +CT DA+ +++CI IS Sbjct: 328 IPLVTSFASQELAICTGAIDAMCVDVQCIMPSIS 361 >3FHG-A nrPDB Length = 207 Score = 25.9 bits (55), Expect = 4.5, Method: Composition-based stats. Identities = 14/45 (31%), Positives = 22/45 (48%) Query: 49 NAAFDNAVQQLNCLNRACAYRKMCATNNLEQAMSVYFTNEQIKEI 93 N++F +A Q LNCL + Y N+ ++ F N + K I Sbjct: 45 NSSFISAYQALNCLGQKIYYANEEEIRNILKSCKYRFYNLKAKYI 89 >6DEO-A nrPDB Length = 682 Score = 25.9 bits (55), Expect = 4.5, Method: Composition-based stats. Identities = 13/38 (34%), Positives = 22/38 (57%) Query: 44 ISPETNAAFDNAVQQLNCLNRACAYRKMCATNNLEQAM 81 I + +A+F+ + +L+ +A A K+C NN EQ M Sbjct: 540 IDIDGDASFNMTLTELSSAVQAGAPIKVCVLNNEEQGM 577 >2LD5-A nrPDB Length = 71 Score = 25.9 bits (55), Expect = 4.8, Method: Composition-based stats. Identities = 10/25 (40%), Positives = 19/25 (76%), Gaps = 1/25 (4%) Query: 69 RKMCATNNL-EQAMSVYFTNEQIKE 92 R++ AT NL E+ ++++F N ++KE Sbjct: 36 RRISATTNLSERQVTIWFQNRRVKE 60 >6FWS-A nrPDB Length = 716 Score = 25.5 bits (54), Expect = 5.6, Method: Composition-based stats. Identities = 20/79 (25%), Positives = 32/79 (40%), Gaps = 21/79 (26%) Query: 15 VASTSAHHQELCTKGDDALV-----TELECIRLR----------ISPETNAAFDN----- 54 +AST Q+L DD L + C +L+ + T+ A D+ Sbjct: 127 LASTEPTQQDLLAFLDDELTPNNQEEQKRCAKLKGDLDTYKWDGLRDHTDIAIDDDLWRR 186 Query: 55 -AVQQLNCLNRACAYRKMC 72 + + +CLNR C Y + C Sbjct: 187 LSTDKASCLNRNCYYYREC 205 >2EWO-A nrPDB Length = 377 Score = 25.1 bits (53), Expect = 7.3, Method: Composition-based stats. Identities = 14/47 (29%), Positives = 21/47 (44%), Gaps = 3/47 (6%) Query: 47 ETNAAFDNAVQQLNCLNRACAYRKMCATNN---LEQAMSVYFTNEQI 90 E AA+D QQ + R KMC T L++A ++ + I Sbjct: 250 EAKAAYDFLSQQTDAKGRPLKVHKMCVTKEPCYLQEAATIDYVEGSI 296 >5OQR-A nrPDB Length = 788 Score = 24.7 bits (52), Expect = 9.2, Method: Composition-based stats. Identities = 23/107 (21%), Positives = 43/107 (40%), Gaps = 8/107 (7%) Query: 4 IFVSALLVVALVASTSAHHQELCTKGDDALVTELECIRLRISPETNAAFDNAVQQLNCLN 63 +++ +L ++ + +H + KG LEC+ L + AF+N L+C Sbjct: 481 LYMVDMLKTLIIPAVRSHDLPIREKG-------LECLSLVCLLNADLAFENVPLYLHCYE 533 Query: 64 RACAYRKMCATNNLEQAMSVYFTNEQIKEIHDAATACDPEAHHEHDH 110 + K A L M + + E DA ++ EA E ++ Sbjct: 534 KGSVVLKCTAIRTLTD-MLIQHGKAKFTEYEDAISSILFEALGEFEN 579 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.128 0.379 Lambda K H 0.267 0.0418 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,830,679 Number of Sequences: 36641 Number of extensions: 174247 Number of successful extensions: 579 Number of sequences better than 10.0: 17 Number of HSP's better than 10.0 without gapping: 21 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 557 Number of HSP's gapped (non-prelim): 35 length of query: 110 length of database: 10,336,785 effective HSP length: 76 effective length of query: 34 effective length of database: 7,552,069 effective search space: 256770346 effective search space used: 256770346 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (25.0 bits)