BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q6TEC1 (212 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2A8R-A nrPDB 431 e-122 3KVH-A nrPDB 192 6e-50 2QJO-A nrPDB 33 0.097 5XXU-U nrPDB 33 0.12 3GZ5-A nrPDB 32 0.24 3N77-A nrPDB 30 0.69 6DT3-A nrPDB 30 0.87 3OGA-A nrPDB 30 0.91 3IGZ-B nrPDB 29 1.2 3EEU-A nrPDB 28 2.2 2RRK-A nrPDB 28 3.5 6M7X-A nrPDB 27 4.3 2ZUE-A nrPDB 27 5.0 4V14-A nrPDB 27 7.0 1UM9-A nrPDB 27 7.0 >2A8R-A nrPDB Length = 212 Score = 431 bits (1109), Expect = e-122, Method: Compositional matrix adjust. Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 1 MAESRSPDRGAKEDKPRPRNISREESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLM 60 MAESRSPDRGAKEDKPRPRNISREESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLM Sbjct: 1 MAESRSPDRGAKEDKPRPRNISREESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLM 60 Query: 61 MMRFDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKC 120 MMRFDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKC Sbjct: 61 MMRFDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKC 120 Query: 121 VTHFYIKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGN 180 VTHFYIKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGN Sbjct: 121 VTHFYIKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGN 180 Query: 181 SKSQLLYALRSLKLLREDQIQEVLKASHRLQY 212 SKSQLLYALRSLKLLREDQIQEVLKASHRLQY Sbjct: 181 SKSQLLYALRSLKLLREDQIQEVLKASHRLQY 212 >3KVH-A nrPDB Length = 214 Score = 192 bits (489), Expect = 6e-50, Method: Compositional matrix adjust. Identities = 95/193 (49%), Positives = 134/193 (69%), Gaps = 5/193 (2%) Query: 16 PRPRNISREESLQL-EGYKHACHALLHAPSQAKLFDRVPIRRVLLMMMRFDGRLGFPGGF 74 P + ISR E+++L G+ H+CHA+L+A + +LF R+P+R +LM MRFDG LGFPGGF Sbjct: 3 PELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGGF 62 Query: 75 VDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKCVTHFYIKELKLEEI 134 VD R SLE+GL R L L + +TE DY SS + E P + V H Y ++L LE++ Sbjct: 63 VDRRFWSLEDGLNRVLGL----GLGCLRLTEADYLSSHLTEGPHRVVAHLYARQLTLEQL 118 Query: 135 ERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGNSKSQLLYALRSLKL 194 +E AV+++DHGLEV+GL+RVPLYT +DRVGG P FL N F+ +K QLL+AL+ L + Sbjct: 119 HAVEISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNM 178 Query: 195 LREDQIQEVLKAS 207 + E+++ E L A+ Sbjct: 179 MPEEKLVEALAAA 191 >2QJO-A nrPDB Length = 341 Score = 33.1 bits (74), Expect = 0.097, Method: Compositional matrix adjust. Identities = 23/70 (32%), Positives = 37/70 (52%), Gaps = 7/70 (10%) Query: 29 LEGYKHACHALLHAPSQAKLFDRVPIRRVLLMMMRFD-----GRLGFPGGFVDTRDISLE 83 L+ YK A +AP+ D V ++ ++M+R G + PGGF+ + +L Sbjct: 187 LQAYKQAWATAPYAPTFITT-DAVVVQAGHVLMVRRQAKPGLGLIALPGGFIKQNE-TLV 244 Query: 84 EGLKRELEEE 93 EG+ REL+EE Sbjct: 245 EGMLRELKEE 254 >5XXU-U nrPDB Length = 233 Score = 32.7 bits (73), Expect = 0.12, Method: Compositional matrix adjust. Identities = 16/34 (47%), Positives = 24/34 (70%), Gaps = 1/34 (2%) Query: 131 LEEIERIEAEAVN-AKDHGLEVMGLIRVPLYTLR 163 L+ IER+ E +N AK+ L+V G +R+P+ TLR Sbjct: 143 LKSIERVCTELINGAKEKNLQVAGPVRLPVKTLR 176 >3GZ5-A nrPDB Length = 240 Score = 31.6 bits (70), Expect = 0.24, Method: Compositional matrix adjust. Identities = 16/31 (51%), Positives = 21/31 (67%), Gaps = 1/31 (3%) Query: 64 FDGRLGFPGGFVD-TRDISLEEGLKRELEEE 93 F G G PGGF+D T D SLE+ + R+L E+ Sbjct: 49 FLGLWGLPGGFIDETCDESLEQTVLRKLAEK 79 >3N77-A nrPDB Length = 144 Score = 30.0 bits (66), Expect = 0.69, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Query: 64 FDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYR 109 F G+ GG V+ + +EE L+RE+ EELG L ++T +R Sbjct: 32 FPGQWALSGGGVEPGE-RIEEALRREIREELGEQLILSDITPWTFR 76 >6DT3-A nrPDB Length = 144 Score = 29.6 bits (65), Expect = 0.87, Method: Compositional matrix adjust. Identities = 18/55 (32%), Positives = 30/55 (54%), Gaps = 2/55 (3%) Query: 64 FDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYR-SSQVREHP 117 F G+ GG V+ + +EE L+RE+ EELG L + +R ++V+ +P Sbjct: 32 FPGQWALSGGGVEPGE-RIEEALRREIREELGEKLILTHIAPWCFRDDTRVKTYP 85 >3OGA-A nrPDB Length = 165 Score = 29.6 bits (65), Expect = 0.91, Method: Compositional matrix adjust. Identities = 17/46 (36%), Positives = 26/46 (56%), Gaps = 1/46 (2%) Query: 64 FDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYR 109 F G+ GG V+ + +EE L+RE+ EELG L ++T +R Sbjct: 53 FPGQWALSGGGVEPGE-RIEEALRREIREELGEQLILSDITPWTFR 97 >3IGZ-B nrPDB Length = 561 Score = 29.3 bits (64), Expect = 1.2, Method: Compositional matrix adjust. Identities = 14/28 (50%), Positives = 18/28 (64%), Gaps = 1/28 (3%) Query: 49 FDRVPIRRV-LLMMMRFDGRLGFPGGFV 75 FDRV + +V MMR+DG LG P F+ Sbjct: 301 FDRVRVPKVRYAGMMRYDGDLGIPNNFL 328 >3EEU-A nrPDB Length = 153 Score = 28.5 bits (62), Expect = 2.2, Method: Compositional matrix adjust. Identities = 15/30 (50%), Positives = 20/30 (66%), Gaps = 1/30 (3%) Query: 66 GRLGFPGGFVDTRDISLEEGLKRELEEELG 95 G+ FPGG ++ + + EE L REL EELG Sbjct: 48 GQWEFPGGKIENGE-TPEEALARELNEELG 76 >2RRK-A nrPDB Length = 140 Score = 27.7 bits (60), Expect = 3.5, Method: Compositional matrix adjust. Identities = 21/50 (42%), Positives = 26/50 (52%), Gaps = 6/50 (12%) Query: 66 GRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVRE 115 G F GG V+ D S + L REL EELG +E T +Y +S RE Sbjct: 35 GLWEFAGGKVEP-DESQRQALVRELREELG-----IEATVGEYVASHQRE 78 >6M7X-A nrPDB Length = 484 Score = 27.3 bits (59), Expect = 4.3, Method: Compositional matrix adjust. Identities = 16/63 (25%), Positives = 31/63 (49%), Gaps = 10/63 (15%) Query: 110 SSQVREHPQKCVTHFYIKELKLEE----------IERIEAEAVNAKDHGLEVMGLIRVPL 159 ++ + EHPQK T + L+E +ER+ + + +++ + L+RV L Sbjct: 325 AASISEHPQKATTELPLLRAALKETLRLYPVGLFLERVASSDLVLQNYHIPAGTLVRVFL 384 Query: 160 YTL 162 Y+L Sbjct: 385 YSL 387 >2ZUE-A nrPDB Length = 629 Score = 27.3 bits (59), Expect = 5.0, Method: Compositional matrix adjust. Identities = 31/98 (31%), Positives = 46/98 (46%), Gaps = 17/98 (17%) Query: 10 GAKEDKPRPRNISREESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLMMMRFDGRLG 69 GA++ P+ ++ + +LQL G++ A L H + V +F GR G Sbjct: 384 GAEQKHPQ---LAIKYALQLLGFEDAAANLYHLAYE----------HVERPEGKFSGRKG 430 Query: 70 FPGGFVDTRDISLEEGLKR--ELEEELGPALATVEVTE 105 GF T D ++E +KR EL EE PAL+ E E Sbjct: 431 TWVGF--TVDEVIQEAVKRARELIEEKNPALSDEEKAE 466 >4V14-A nrPDB Length = 138 Score = 26.9 bits (58), Expect = 7.0, Method: Compositional matrix adjust. Identities = 18/40 (45%), Positives = 24/40 (60%), Gaps = 6/40 (15%) Query: 66 GRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTE 105 G FPGG V+ + S E+ + RELEEE+G + VTE Sbjct: 38 GFWEFPGGKVEAGE-SREQAMVRELEEEIG-----ITVTE 71 >1UM9-A nrPDB Length = 367 Score = 26.9 bits (58), Expect = 7.0, Method: Compositional matrix adjust. Identities = 21/59 (35%), Positives = 26/59 (44%), Gaps = 3/59 (5%) Query: 67 RLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKCVTHFY 125 R GF F RD L L L+E LG LAT D + Q+ EHP +F+ Sbjct: 85 RPGFDWVFPYYRDHGLALALGIPLKELLGQMLAT---KADPNKGRQMPEHPGSKALNFF 140 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2A8R-A nrPDB 360 e-100 3KVH-A nrPDB 302 6e-83 Sequences not found previously or not previously below threshold: 2QJO-A nrPDB 34 0.037 5XXB-D nrPDB 32 0.19 3VIK-A nrPDB 30 0.91 6CL4-A nrPDB 30 0.92 4C0D-A nrPDB 28 2.3 3GZ5-A nrPDB 28 2.3 5XXU-U nrPDB 27 4.8 5IKK-A nrPDB 27 5.5 3IGZ-B nrPDB 26 8.7 CONVERGED! >2A8R-A nrPDB Length = 212 Score = 360 bits (923), Expect = e-100, Method: Composition-based stats. Identities = 212/212 (100%), Positives = 212/212 (100%) Query: 1 MAESRSPDRGAKEDKPRPRNISREESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLM 60 MAESRSPDRGAKEDKPRPRNISREESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLM Sbjct: 1 MAESRSPDRGAKEDKPRPRNISREESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLM 60 Query: 61 MMRFDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKC 120 MMRFDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKC Sbjct: 61 MMRFDGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKC 120 Query: 121 VTHFYIKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGN 180 VTHFYIKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGN Sbjct: 121 VTHFYIKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGN 180 Query: 181 SKSQLLYALRSLKLLREDQIQEVLKASHRLQY 212 SKSQLLYALRSLKLLREDQIQEVLKASHRLQY Sbjct: 181 SKSQLLYALRSLKLLREDQIQEVLKASHRLQY 212 >3KVH-A nrPDB Length = 214 Score = 302 bits (774), Expect = 6e-83, Method: Composition-based stats. Identities = 97/197 (49%), Positives = 135/197 (68%), Gaps = 5/197 (2%) Query: 16 PRPRNISREESLQL-EGYKHACHALLHAPSQAKLFDRVPIRRVLLMMMRFDGRLGFPGGF 74 P + ISR E+++L G+ H+CHA+L+A + +LF R+P+R +LM MRFDG LGFPGGF Sbjct: 3 PELKQISRVEAMRLGPGWSHSCHAMLYAANPGQLFGRIPMRFSVLMQMRFDGLLGFPGGF 62 Query: 75 VDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKCVTHFYIKELKLEEI 134 VD R SLE+GL R L LG +TE DY SS + E P + V H Y ++L LE++ Sbjct: 63 VDRRFWSLEDGLNRVLGLGLGCL----RLTEADYLSSHLTEGPHRVVAHLYARQLTLEQL 118 Query: 135 ERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGNSKSQLLYALRSLKL 194 +E AV+++DHGLEV+GL+RVPLYT +DRVGG P FL N F+ +K QLL+AL+ L + Sbjct: 119 HAVEISAVHSRDHGLEVLGLVRVPLYTQKDRVGGFPNFLSNAFVSTAKCQLLFALKVLNM 178 Query: 195 LREDQIQEVLKASHRLQ 211 + E+++ E L A+ Q Sbjct: 179 MPEEKLVEALAAATEKQ 195 >2QJO-A nrPDB Length = 341 Score = 34.2 bits (77), Expect = 0.037, Method: Composition-based stats. Identities = 24/101 (23%), Positives = 42/101 (41%), Gaps = 2/101 (1%) Query: 66 GRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKCVTHFY 125 G + PGGF+ + +L EG+ REL+EE + + V S V + P + + Sbjct: 228 GLIALPGGFIKQNE-TLVEGMLRELKEETRLKVP-LPVLRGSIVDSHVFDAPGRSLRGRT 285 Query: 126 IKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRV 166 I ++ E AV D + + LY +++ Sbjct: 286 ITHAYFIQLPGGELPAVKGGDDAQKAWWMSLADLYAQEEQI 326 >5XXB-D nrPDB Length = 310 Score = 31.9 bits (71), Expect = 0.19, Method: Composition-based stats. Identities = 28/141 (19%), Positives = 50/141 (35%), Gaps = 6/141 (4%) Query: 65 DGRLGFPGGFVDTRDISLEEGLKRELEEELGPALATVEVTEDDYRSSQVREHPQKCVTHF 124 DG L P G S EG + + + DY E P+K F Sbjct: 167 DGGLHVPHGIKKFPGYSKPEGEGEGSYDPEAHRARILGLHVADYMRQLKEEDPEKYSAQF 226 Query: 125 YIKELKLEEIERIEAEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGNSKSQ 184 + +IE + EA+ H + + P ++R + N I +K Q Sbjct: 227 --SQYIKNKIEADDIEAMYKNAHA----QIRKNPDAVKKERAANVQNVRQGNVIKTAKGQ 280 Query: 185 LLYALRSLKLLREDQIQEVLK 205 + ++ K R +++Q+ + Sbjct: 281 YVRHVKLTKAQRRERVQQKIA 301 >3VIK-A nrPDB Length = 487 Score = 29.6 bits (65), Expect = 0.91, Method: Composition-based stats. Identities = 26/147 (17%), Positives = 51/147 (34%), Gaps = 30/147 (20%) Query: 80 ISLEEGLKRELEEELG---PALATVEVTEDDYRSSQVREHPQKCVTHFYIKELKLEEIER 136 ++ +G E+ P + DY ++ H + H Y +E + E+ + Sbjct: 176 LTFMDGYASEIGMAPSINTPGIG-------DYLAAHTVIHAHARIYHLYDQEFRAEQGGK 228 Query: 137 IEAE--------AVNAKDHGLEV-------MGLIRVPLYTLRDRVGGLPAFLCNNFIGNS 181 + A N+ + +GL P++T G PA L + NS Sbjct: 229 VGISLNINWCEPATNSAEDRASCENYQQFNLGLYAHPIFTEE---GDYPAVLKDRVSRNS 285 Query: 182 KSQLLYALRSLKLLREDQIQEVLKASH 208 + R + E+ E ++ +H Sbjct: 286 ADEGYTDSRLPQFTAEE--VEYIRGTH 310 >6CL4-A nrPDB Length = 313 Score = 29.6 bits (65), Expect = 0.92, Method: Composition-based stats. Identities = 23/68 (33%), Positives = 32/68 (47%), Gaps = 7/68 (10%) Query: 139 AEAVNAKDHGLEVMGLIRVPLYTLRDRVGGLPAFLCNNFIGNSKSQLLYALRSLKLLRED 198 +VN +HG EV L+R+ L G LP + N + + QLL AL L + Sbjct: 123 VTSVNGVNHGSEVADLVRLALTP-----GRLPESIANAAMS-AFGQLLSALAGSPRLPQS 176 Query: 199 QIQEVLKA 206 I E L+A Sbjct: 177 GI-EALEA 183 >4C0D-A nrPDB Length = 812 Score = 28.4 bits (62), Expect = 2.3, Method: Composition-based stats. Identities = 17/67 (25%), Positives = 32/67 (47%), Gaps = 3/67 (4%) Query: 118 QKCVTHFYIKEL--KLEEIERIEAEAVNAKDHGL-EVMGLIRVPLYTLRDRVGGLPAFLC 174 ++ V H +L +E + RI A + GL ++M ++R + DR G P F+ Sbjct: 214 ERSVAHVTEADLFHTIETLMRINAHSRGNAPEGLPQLMEVVRSNYEAMIDRAHGGPNFMM 273 Query: 175 NNFIGNS 181 ++ I + Sbjct: 274 HSGISQA 280 >3GZ5-A nrPDB Length = 240 Score = 28.4 bits (62), Expect = 2.3, Method: Composition-based stats. Identities = 16/31 (51%), Positives = 21/31 (67%), Gaps = 1/31 (3%) Query: 64 FDGRLGFPGGFVD-TRDISLEEGLKRELEEE 93 F G G PGGF+D T D SLE+ + R+L E+ Sbjct: 49 FLGLWGLPGGFIDETCDESLEQTVLRKLAEK 79 >5XXU-U nrPDB Length = 233 Score = 27.3 bits (59), Expect = 4.8, Method: Composition-based stats. Identities = 18/47 (38%), Positives = 27/47 (57%), Gaps = 3/47 (6%) Query: 118 QKCVTHFYIKELKLEEIERIEAEAVN-AKDHGLEVMGLIRVPLYTLR 163 + K+LK IER+ E +N AK+ L+V G +R+P+ TLR Sbjct: 132 HRIRITLTSKDLK--SIERVCTELINGAKEKNLQVAGPVRLPVKTLR 176 >5IKK-A nrPDB Length = 657 Score = 27.3 bits (59), Expect = 5.5, Method: Composition-based stats. Identities = 15/52 (28%), Positives = 26/52 (50%), Gaps = 8/52 (15%) Query: 13 EDKPR-PRNISRE-ESLQLEGYKHACHALLHAPSQAKLFDRVPIRRVLLMMM 62 +D P PR + R E+++ GY + + PS + +F R+P R L + Sbjct: 45 DDHPEDPRRVLRVFEAIKKAGY------VSNVPSPSDVFLRIPAREATLEEL 90 >3IGZ-B nrPDB Length = 561 Score = 26.5 bits (57), Expect = 8.7, Method: Composition-based stats. Identities = 14/28 (50%), Positives = 18/28 (64%), Gaps = 1/28 (3%) Query: 49 FDRVPIRRVLLM-MMRFDGRLGFPGGFV 75 FDRV + +V MMR+DG LG P F+ Sbjct: 301 FDRVRVPKVRYAGMMRYDGDLGIPNNFL 328 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.141 0.401 Lambda K H 0.267 0.0433 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,538,317 Number of Sequences: 36641 Number of extensions: 355163 Number of successful extensions: 786 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 27 Number of HSP's that attempted gapping in prelim test: 771 Number of HSP's gapped (non-prelim): 39 length of query: 212 length of database: 10,336,785 effective HSP length: 93 effective length of query: 119 effective length of database: 6,929,172 effective search space: 824571468 effective search space used: 824571468 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 57 (26.5 bits)