BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q6LZY8 (271 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4CAD-C nrPDB 531 e-151 >4CAD-C nrPDB Length = 271 Score = 531 bits (1367), Expect = e-151, Method: Compositional matrix adjust. Identities = 270/271 (99%), Positives = 271/271 (100%) Query: 1 MISSYKYNPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIA 60 MISSYKYNPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIA Sbjct: 1 MISSYKYNPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIA 60 Query: 61 KSKNNELKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPFGGSISQFQFSGGF 120 KSKNN+LKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPFGGSISQFQFSGGF Sbjct: 61 KSKNNDLKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPFGGSISQFQFSGGF 120 Query: 121 SFSTDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVN 180 SFSTDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVN Sbjct: 121 SFSTDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVN 180 Query: 181 NSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAIT 240 NSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAIT Sbjct: 181 NSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAIT 240 Query: 241 QDTKIIETGVLFLVAAAIILYDKKMFFEKLG 271 QDTKIIETGVLFLVAAAIILYDKKMFFEKLG Sbjct: 241 QDTKIIETGVLFLVAAAIILYDKKMFFEKLG 271 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4CAD-C nrPDB 408 e-114 Sequences not found previously or not previously below threshold: 2FTW-A nrPDB 28 5.4 CONVERGED! >4CAD-C nrPDB Length = 271 Score = 408 bits (1049), Expect = e-114, Method: Composition-based stats. Identities = 270/271 (99%), Positives = 271/271 (100%) Query: 1 MISSYKYNPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIA 60 MISSYKYNPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIA Sbjct: 1 MISSYKYNPKLYFLSTFVVTYILWFTGAYLSFSSTYSGIYMLIMLPGLMAPFIISTILIA 60 Query: 61 KSKNNELKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPFGGSISQFQFSGGF 120 KSKNN+LKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPFGGSISQFQFSGGF Sbjct: 61 KSKNNDLKKDFINRLFNLKLINLKTIPVVFLLMPAVILLSILLSIPFGGSISQFQFSGGF 120 Query: 121 SFSTDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVN 180 SFSTDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVN Sbjct: 121 SFSTDFVPVLFLLLLAATFEELGWRGYAFDSLQSRYSLFKASILFGIFWSLWHFPLIFVN 180 Query: 181 NSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAIT 240 NSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAIT Sbjct: 181 NSYQYEIFNQSIWYGLNFFLSILPMGIIITWMCLKNRKSIILAIIFHFLINLNQELLAIT 240 Query: 241 QDTKIIETGVLFLVAAAIILYDKKMFFEKLG 271 QDTKIIETGVLFLVAAAIILYDKKMFFEKLG Sbjct: 241 QDTKIIETGVLFLVAAAIILYDKKMFFEKLG 271 >2FTW-A nrPDB Length = 521 Score = 27.9 bits (61), Expect = 5.4, Method: Composition-based stats. Identities = 25/80 (31%), Positives = 36/80 (45%), Gaps = 12/80 (15%) Query: 91 LLMPAVILLSILLSIPFGGSIS--------QFQFSGGFSFSTDFV-PVLFLLLLAATFEE 141 LL+P I +PF G++S Q +GG +F DFV P LL A + Sbjct: 57 LLLPGGIDTHTHFQLPFMGTVSVDDFDIGTQAAVAGGTTFIIDFVIPTRGQSLLEAYDQ- 115 Query: 142 LGWRGYAFDSLQSRYSLFKA 161 W+ +A + + YSL A Sbjct: 116 --WKKWADEKVNCDYSLHVA 133 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.327 0.153 0.496 Lambda K H 0.267 0.0478 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 13,168,653 Number of Sequences: 36641 Number of extensions: 647093 Number of successful extensions: 1598 Number of sequences better than 10.0: 22 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 31 Number of HSP's that attempted gapping in prelim test: 1590 Number of HSP's gapped (non-prelim): 37 length of query: 271 length of database: 10,336,785 effective HSP length: 95 effective length of query: 176 effective length of database: 6,855,890 effective search space: 1206636640 effective search space used: 1206636640 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.6 bits) S2: 59 (27.1 bits)