BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q6JP77 (353 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3J4Q-A nrPDB 726 0.0 6HIW-DQ nrPDB 57 1e-08 1SU3-A nrPDB 30 1.1 3RIU-C nrPDB 30 1.6 5MRE-B nrPDB 29 3.1 >3J4Q-A nrPDB Length = 353 Score = 726 bits (1875), Expect = 0.0, Method: Compositional matrix adjust. Identities = 353/353 (100%), Positives = 353/353 (100%) Query: 1 MERPAAGEIDANKCDHLSRGEEGTGDLETSPVGSLADLPFAAVDIQDDCGLPDVPQGNVP 60 MERPAAGEIDANKCDHLSRGEEGTGDLETSPVGSLADLPFAAVDIQDDCGLPDVPQGNVP Sbjct: 1 MERPAAGEIDANKCDHLSRGEEGTGDLETSPVGSLADLPFAAVDIQDDCGLPDVPQGNVP 60 Query: 61 QGNPKRSKENRGDRNDHVKKRKKAKKDYQPNYFLSIPITNKKITAGIKVLQNSILRQDNR 120 QGNPKRSKENRGDRNDHVKKRKKAKKDYQPNYFLSIPITNKKITAGIKVLQNSILRQDNR Sbjct: 61 QGNPKRSKENRGDRNDHVKKRKKAKKDYQPNYFLSIPITNKKITAGIKVLQNSILRQDNR 120 Query: 121 LTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ 180 LTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ Sbjct: 121 LTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ 180 Query: 181 GQVGFVKLADGDHVSALLEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAPMLWKKG 240 GQVGFVKLADGDHVSALLEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAPMLWKKG Sbjct: 181 GQVGFVKLADGDHVSALLEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAPMLWKKG 240 Query: 241 VRKIEPGLYEQFIDHRFGEEILYQIDLCSMLKKKQSNGYYHCESSIVIGEKDRKEPEDAE 300 VRKIEPGLYEQFIDHRFGEEILYQIDLCSMLKKKQSNGYYHCESSIVIGEKDRKEPEDAE Sbjct: 241 VRKIEPGLYEQFIDHRFGEEILYQIDLCSMLKKKQSNGYYHCESSIVIGEKDRKEPEDAE 300 Query: 301 LVRLSKRLVENAVLKAVQQYLEETQNKKQPGEGNSVKAEEGDRNGDGSDNNRK 353 LVRLSKRLVENAVLKAVQQYLEETQNKKQPGEGNSVKAEEGDRNGDGSDNNRK Sbjct: 301 LVRLSKRLVENAVLKAVQQYLEETQNKKQPGEGNSVKAEEGDRNGDGSDNNRK 353 >6HIW-DQ nrPDB Length = 268 Score = 57.0 bits (136), Expect = 1e-08, Method: Compositional matrix adjust. Identities = 51/203 (25%), Positives = 91/203 (44%), Gaps = 12/203 (5%) Query: 89 QPNYFLSIPITNK-KITAGIKVLQNSILRQDNRLTKAMVGDGSFHITLLVMQLLNEDEVN 147 +P +F S+ + ++ +T +K + N +L + +V HITL VM + Sbjct: 70 RPTHFFSLRLPSRNSVTLRVKEMHNHLLFSHQQCAPMLVSPTKLHITLGVMTIPENSREE 129 Query: 148 IGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ-GQVGFVKLA-DGDHVSALLEIAETAK 205 + + + E + L L F G+GTF G+V F+++ + D + + + Sbjct: 130 LVKNIQQSMS---ETFACVQPLQLRFRGLGTFDFGRVLFIRVVPEADFGRLERAVCDIRR 186 Query: 206 RTFQEKGI-LAGESR-TFKPHLTFMKLSKAPMLWKKGVRKIEPGLYEQFIDHRFGEEILY 263 R G+ L G ++ PH+T K+ P ++ I L+ + H FG+ L Sbjct: 187 RVGGVLGVDLKGNPHDSYVPHVTVAKIR--PNQRQQLGDTIPQSLWADYQHHDFGDVTLS 244 Query: 264 QIDLCSMLKKKQSNGYYHCESSI 286 +DLC M K +GYYH E ++ Sbjct: 245 TVDLCRM--KGSPDGYYHTEGAV 265 >1SU3-A nrPDB Length = 450 Score = 30.4 bits (67), Expect = 1.1, Method: Compositional matrix adjust. Identities = 23/90 (25%), Positives = 40/90 (44%), Gaps = 6/90 (6%) Query: 46 QDDCGLPDVPQGNVPQGNPKRSKENRGDR-NDHVKKRKKAKKDYQPNYFLSI-----PIT 99 Q CG+PDV Q + +GNP+ + + R ++ +A D+ + P+T Sbjct: 70 QPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLT 129 Query: 100 NKKITAGIKVLQNSILRQDNRLTKAMVGDG 129 K++ G + S +R D+R G G Sbjct: 130 FTKVSEGQADIMISFVRGDHRDNSPFDGPG 159 >3RIU-C nrPDB Length = 269 Score = 30.0 bits (66), Expect = 1.6, Method: Compositional matrix adjust. Identities = 18/43 (41%), Positives = 28/43 (65%), Gaps = 1/43 (2%) Query: 297 EDAELVRLSKRLVENAVLKAVQQYLEE-TQNKKQPGEGNSVKA 338 EDAE +K + + ++AV QY+EE +Q K++P EG V+A Sbjct: 108 EDAEGENETKSVSDWQAIQAVMQYVEESSQPKEEPTEGEDVQA 150 >5MRE-B nrPDB Length = 393 Score = 29.3 bits (64), Expect = 3.1, Method: Compositional matrix adjust. Identities = 15/37 (40%), Positives = 18/37 (48%), Gaps = 3/37 (8%) Query: 3 RPAAGEIDANKCDHL---SRGEEGTGDLETSPVGSLA 36 RP + NKCDH RG+ + L SP G LA Sbjct: 330 RPTVRGVAMNKCDHPHGGGRGKSKSNKLSMSPWGQLA 366 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3J4Q-A nrPDB 654 0.0 6HIW-DQ nrPDB 301 2e-82 Sequences not found previously or not previously below threshold: 3RIU-C nrPDB 34 0.076 1SU3-A nrPDB 32 0.38 6BOY-B nrPDB 30 1.5 1KA4-A nrPDB 29 2.4 CONVERGED! >3J4Q-A nrPDB Length = 353 Score = 654 bits (1688), Expect = 0.0, Method: Composition-based stats. Identities = 353/353 (100%), Positives = 353/353 (100%) Query: 1 MERPAAGEIDANKCDHLSRGEEGTGDLETSPVGSLADLPFAAVDIQDDCGLPDVPQGNVP 60 MERPAAGEIDANKCDHLSRGEEGTGDLETSPVGSLADLPFAAVDIQDDCGLPDVPQGNVP Sbjct: 1 MERPAAGEIDANKCDHLSRGEEGTGDLETSPVGSLADLPFAAVDIQDDCGLPDVPQGNVP 60 Query: 61 QGNPKRSKENRGDRNDHVKKRKKAKKDYQPNYFLSIPITNKKITAGIKVLQNSILRQDNR 120 QGNPKRSKENRGDRNDHVKKRKKAKKDYQPNYFLSIPITNKKITAGIKVLQNSILRQDNR Sbjct: 61 QGNPKRSKENRGDRNDHVKKRKKAKKDYQPNYFLSIPITNKKITAGIKVLQNSILRQDNR 120 Query: 121 LTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ 180 LTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ Sbjct: 121 LTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ 180 Query: 181 GQVGFVKLADGDHVSALLEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAPMLWKKG 240 GQVGFVKLADGDHVSALLEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAPMLWKKG Sbjct: 181 GQVGFVKLADGDHVSALLEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAPMLWKKG 240 Query: 241 VRKIEPGLYEQFIDHRFGEEILYQIDLCSMLKKKQSNGYYHCESSIVIGEKDRKEPEDAE 300 VRKIEPGLYEQFIDHRFGEEILYQIDLCSMLKKKQSNGYYHCESSIVIGEKDRKEPEDAE Sbjct: 241 VRKIEPGLYEQFIDHRFGEEILYQIDLCSMLKKKQSNGYYHCESSIVIGEKDRKEPEDAE 300 Query: 301 LVRLSKRLVENAVLKAVQQYLEETQNKKQPGEGNSVKAEEGDRNGDGSDNNRK 353 LVRLSKRLVENAVLKAVQQYLEETQNKKQPGEGNSVKAEEGDRNGDGSDNNRK Sbjct: 301 LVRLSKRLVENAVLKAVQQYLEETQNKKQPGEGNSVKAEEGDRNGDGSDNNRK 353 >6HIW-DQ nrPDB Length = 268 Score = 301 bits (772), Expect = 2e-82, Method: Composition-based stats. Identities = 51/203 (25%), Positives = 91/203 (44%), Gaps = 12/203 (5%) Query: 89 QPNYFLSIPITNK-KITAGIKVLQNSILRQDNRLTKAMVGDGSFHITLLVMQLLNEDEVN 147 +P +F S+ + ++ +T +K + N +L + +V HITL VM + Sbjct: 70 RPTHFFSLRLPSRNSVTLRVKEMHNHLLFSHQQCAPMLVSPTKLHITLGVMTIPENSREE 129 Query: 148 IGTDALLELKPFVEEILEGKHLTLPFHGIGTFQ-GQVGFVKLA-DGDHVSALLEIAETAK 205 + + + E + L L F G+GTF G+V F+++ + D + + + Sbjct: 130 LVKNIQQSMS---ETFACVQPLQLRFRGLGTFDFGRVLFIRVVPEADFGRLERAVCDIRR 186 Query: 206 RTFQEKGI-LAGESR-TFKPHLTFMKLSKAPMLWKKGVRKIEPGLYEQFIDHRFGEEILY 263 R G+ L G ++ PH+T K+ P ++ I L+ + H FG+ L Sbjct: 187 RVGGVLGVDLKGNPHDSYVPHVTVAKIR--PNQRQQLGDTIPQSLWADYQHHDFGDVTLS 244 Query: 264 QIDLCSMLKKKQSNGYYHCESSI 286 +DLC M K +GYYH E ++ Sbjct: 245 TVDLCRM--KGSPDGYYHTEGAV 265 >3RIU-C nrPDB Length = 269 Score = 34.3 bits (77), Expect = 0.076, Method: Composition-based stats. Identities = 18/43 (41%), Positives = 28/43 (65%), Gaps = 1/43 (2%) Query: 297 EDAELVRLSKRLVENAVLKAVQQYLEE-TQNKKQPGEGNSVKA 338 EDAE +K + + ++AV QY+EE +Q K++P EG V+A Sbjct: 108 EDAEGENETKSVSDWQAIQAVMQYVEESSQPKEEPTEGEDVQA 150 >1SU3-A nrPDB Length = 450 Score = 32.0 bits (71), Expect = 0.38, Method: Composition-based stats. Identities = 23/90 (25%), Positives = 40/90 (44%), Gaps = 6/90 (6%) Query: 46 QDDCGLPDVPQGNVPQGNPKRSKENRGDR-NDHVKKRKKAKKDYQPNYFLSI-----PIT 99 Q CG+PDV Q + +GNP+ + + R ++ +A D+ + P+T Sbjct: 70 QPRCGVPDVAQFVLTEGNPRWEQTHLTYRIENYTPDLPRADVDHAIEKAFQLWSNVTPLT 129 Query: 100 NKKITAGIKVLQNSILRQDNRLTKAMVGDG 129 K++ G + S +R D+R G G Sbjct: 130 FTKVSEGQADIMISFVRGDHRDNSPFDGPG 159 >6BOY-B nrPDB Length = 463 Score = 30.0 bits (66), Expect = 1.5, Method: Composition-based stats. Identities = 28/157 (17%), Positives = 60/157 (38%), Gaps = 13/157 (8%) Query: 82 KKAKKDYQPNYFLSIPITNKKITAGIKVLQNSILRQDNRLTKAMVGDGSFHITL----LV 137 K+AKK N+ S+P ++ + A ++ L D+ V I + L Sbjct: 60 KEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLP 119 Query: 138 MQLLNEDEVNIGTDALLELKPFVEEILEGKHLTLPFHGIGTFQGQVGFVKLADGDHVSAL 197 +QL + EV++ + + + + F +L ++ GT + + D + Sbjct: 120 LQLFHPQEVSMVRNLIQKDRTFA--VLAYSNVQEREAQFGT--TAEIYAYREEQDFGIEI 175 Query: 198 LEIAETAKRTFQEKGILAGESRTFKPHLTFMKLSKAP 234 +++ ++ F+ E RT + K+ P Sbjct: 176 VKVKAIGRQRFKVL-----ELRTQSDGIQQAKVQILP 207 >1KA4-A nrPDB Length = 499 Score = 29.3 bits (64), Expect = 2.4, Method: Composition-based stats. Identities = 15/100 (15%), Positives = 35/100 (35%), Gaps = 5/100 (5%) Query: 101 KKITAGIKVLQNSILRQDNRLTKAMVGDGSFHITLLVMQLLNEDEVNIGTDALLELKPFV 160 + I + + L + + + M + ++L + +E + + + K FV Sbjct: 262 RTILSTVHEF-GHALYELQQDERFMFTPIAGGVSLGI----HESQSRFWENIIGRSKEFV 316 Query: 161 EEILEGKHLTLPFHGIGTFQGQVGFVKLADGDHVSALLEI 200 E I LPF T + + + D + ++ Sbjct: 317 ELIYPVLKENLPFMSNYTPEDVYLYFNIVRPDFIRTEADV 356 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.312 0.133 0.362 Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,313,807 Number of Sequences: 36641 Number of extensions: 592573 Number of successful extensions: 1007 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 995 Number of HSP's gapped (non-prelim): 22 length of query: 353 length of database: 10,336,785 effective HSP length: 98 effective length of query: 255 effective length of database: 6,745,967 effective search space: 1720221585 effective search space used: 1720221585 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.8 bits) S2: 60 (27.7 bits)