BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q6ID70 (150 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2KMW-A nrPDB 305 7e-84 2RH0-A nrPDB 32 0.15 5J46-A nrPDB 29 0.76 1LYL-A nrPDB 27 4.3 >2KMW-A nrPDB Length = 150 Score = 305 bits (780), Expect = 7e-84, Method: Compositional matrix adjust. Identities = 149/149 (100%), Positives = 149/149 (100%) Query: 2 SRNPEVLWAQRSDKVYLTVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLELYGKIM 61 SRNPEVLWAQRSDKVYLTVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLELYGKIM Sbjct: 2 SRNPEVLWAQRSDKVYLTVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLELYGKIM 61 Query: 62 TEYRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIKVDWNKWCDEDEEVNSETASDD 121 TEYRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIKVDWNKWCDEDEEVNSETASDD Sbjct: 62 TEYRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIKVDWNKWCDEDEEVNSETASDD 121 Query: 122 ESAFVNQDSESSDDDGLLYLPDLEKARNK 150 ESAFVNQDSESSDDDGLLYLPDLEKARNK Sbjct: 122 ESAFVNQDSESSDDDGLLYLPDLEKARNK 150 >2RH0-A nrPDB Length = 157 Score = 31.6 bits (70), Expect = 0.15, Method: Compositional matrix adjust. Identities = 24/97 (24%), Positives = 48/97 (49%), Gaps = 7/97 (7%) Query: 9 WAQRSDKVYLTVALP-DAKDISVKCEPQGLFSFSALGA----QGERFEFSLELYGKIMTE 63 W Q ++V++ V +P + ++C Q A+G +G+ F+ ++ G E Sbjct: 18 WYQTLEEVFIEVQVPPGTRAQDIQCGLQSRHVALAVGGREILKGKLFDSTIADEGTWTLE 77 Query: 64 YRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIK 100 RK V R ++ +++ + WT LL+SE P+++ Sbjct: 78 DRKMV--RIVLTKTKRDAANCWTSLLESEYAADPWVQ 112 >5J46-A nrPDB Length = 189 Score = 29.3 bits (64), Expect = 0.76, Method: Compositional matrix adjust. Identities = 25/96 (26%), Positives = 39/96 (40%), Gaps = 18/96 (18%) Query: 4 NPEVLWAQRSDKVY--LTVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLE------ 55 NPE++W+ +VY +++P D + E AL QGE FE E Sbjct: 97 NPEIIWSSDGKQVYEEGCLSVPGIYD---EVERPDRVRVRALNEQGETFELDCEGLLAVC 153 Query: 56 -------LYGKIMTEYRKNVGLRNIIFSIQKEERSW 84 L G++ EY + I ++K ER+ Sbjct: 154 IQHEMDHLMGRVFVEYLSPLKQSRIKTKMKKLERAM 189 >1LYL-A nrPDB Length = 504 Score = 26.6 bits (57), Expect = 4.3, Method: Composition-based stats. Identities = 11/28 (39%), Positives = 20/28 (71%) Query: 111 EEVNSETASDDESAFVNQDSESSDDDGL 138 E+VN++ A DDE+ F ++D ++ + GL Sbjct: 441 EQVNAKAAGDDEAMFYDEDYVTALEYGL 468 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2KMW-A nrPDB 308 6e-85 Sequences not found previously or not previously below threshold: 2RH0-A nrPDB 35 0.016 5J46-A nrPDB 33 0.067 3AAF-A nrPDB 28 1.5 1LYL-A nrPDB 28 1.6 6J6H-n nrPDB 28 1.7 3QFL-A nrPDB 27 2.2 5H8W-A nrPDB 27 2.9 CONVERGED! >2KMW-A nrPDB Length = 150 Score = 308 bits (789), Expect = 6e-85, Method: Composition-based stats. Identities = 149/149 (100%), Positives = 149/149 (100%) Query: 2 SRNPEVLWAQRSDKVYLTVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLELYGKIM 61 SRNPEVLWAQRSDKVYLTVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLELYGKIM Sbjct: 2 SRNPEVLWAQRSDKVYLTVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLELYGKIM 61 Query: 62 TEYRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIKVDWNKWCDEDEEVNSETASDD 121 TEYRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIKVDWNKWCDEDEEVNSETASDD Sbjct: 62 TEYRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIKVDWNKWCDEDEEVNSETASDD 121 Query: 122 ESAFVNQDSESSDDDGLLYLPDLEKARNK 150 ESAFVNQDSESSDDDGLLYLPDLEKARNK Sbjct: 122 ESAFVNQDSESSDDDGLLYLPDLEKARNK 150 >2RH0-A nrPDB Length = 157 Score = 34.7 bits (78), Expect = 0.016, Method: Composition-based stats. Identities = 27/99 (27%), Positives = 50/99 (50%), Gaps = 11/99 (11%) Query: 9 WAQRSDKVYLTVALPD---AKDISVKCEPQGLFSFSALGA----QGERFEFSLELYGKIM 61 W Q ++V++ V +P A+DI +C Q A+G +G+ F+ ++ G Sbjct: 18 WYQTLEEVFIEVQVPPGTRAQDI--QCGLQSRHVALAVGGREILKGKLFDSTIADEGTWT 75 Query: 62 TEYRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIK 100 E RK V R ++ +++ + WT LL+SE P+++ Sbjct: 76 LEDRKMV--RIVLTKTKRDAANCWTSLLESEYAADPWVQ 112 >5J46-A nrPDB Length = 189 Score = 32.8 bits (73), Expect = 0.067, Method: Composition-based stats. Identities = 25/95 (26%), Positives = 39/95 (41%), Gaps = 18/95 (18%) Query: 4 NPEVLWAQRSDKVYL--TVALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLE------ 55 NPE++W+ +VY +++P D + E AL QGE FE E Sbjct: 97 NPEIIWSSDGKQVYEEGCLSVPGIYD---EVERPDRVRVRALNEQGETFELDCEGLLAVC 153 Query: 56 -------LYGKIMTEYRKNVGLRNIIFSIQKEERS 83 L G++ EY + I ++K ER+ Sbjct: 154 IQHEMDHLMGRVFVEYLSPLKQSRIKTKMKKLERA 188 >3AAF-A nrPDB Length = 134 Score = 28.1 bits (61), Expect = 1.5, Method: Composition-based stats. Identities = 19/74 (25%), Positives = 33/74 (44%), Gaps = 13/74 (17%) Query: 20 VALPDAKDISVKCEPQGLFSFSALGAQGERFEFSLELY-------GKIMTEYRKNVGLRN 72 + + D++D S PQ SA+ GE+F L + ++ +YR R+ Sbjct: 2 IRMDDSEDTSWDFGPQAFKLLSAVDILGEKFGIGLPILFLRGSNSQRLADQYR-----RH 56 Query: 73 IIFSIQKEE-RSWW 85 +F K++ SWW Sbjct: 57 SLFGTGKDQTESWW 70 >1LYL-A nrPDB Length = 504 Score = 28.1 bits (61), Expect = 1.6, Method: Composition-based stats. Identities = 11/28 (39%), Positives = 20/28 (71%) Query: 111 EEVNSETASDDESAFVNQDSESSDDDGL 138 E+VN++ A DDE+ F ++D ++ + GL Sbjct: 441 EQVNAKAAGDDEAMFYDEDYVTALEYGL 468 >6J6H-n nrPDB Length = 455 Score = 28.1 bits (61), Expect = 1.7, Method: Composition-based stats. Identities = 12/35 (34%), Positives = 19/35 (54%), Gaps = 2/35 (5%) Query: 103 WNKWCDEDEEVN--SETASDDESAFVNQDSESSDD 135 W W D+E + SET +D+ FV+ +E + D Sbjct: 69 WGSWSSSDDETSQASETQKEDQDIFVHALAEDNLD 103 >3QFL-A nrPDB Length = 115 Score = 27.4 bits (59), Expect = 2.2, Method: Composition-based stats. Identities = 21/80 (26%), Positives = 36/80 (45%), Gaps = 1/80 (1%) Query: 58 GKIMTE-YRKNVGLRNIIFSIQKEERSWWTRLLKSEEKPAPYIKVDWNKWCDEDEEVNSE 116 G+++TE ++ + G++ I + KE S L+K E P + W DE E++ Sbjct: 11 GELLTEEFKLHKGVKKNIEDLGKELESMNAALIKIGEVPREQLDSQDKLWADEVRELSYV 70 Query: 117 TASDDESAFVNQDSESSDDD 136 + V D SDD+ Sbjct: 71 IEDVVDKFLVQVDGIKSDDN 90 >5H8W-A nrPDB Length = 597 Score = 27.4 bits (59), Expect = 2.9, Method: Composition-based stats. Identities = 17/43 (39%), Positives = 21/43 (48%) Query: 40 FSALGAQGERFEFSLELYGKIMTEYRKNVGLRNIIFSIQKEER 82 +AL GE E E GK+ Y +V R I FS Q EE+ Sbjct: 286 LNALYLFGEYVENEKEKVGKVPFSYASSVASRIIAFSDQDEEK 328 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.131 0.389 Lambda K H 0.267 0.0412 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,859,583 Number of Sequences: 36641 Number of extensions: 219793 Number of successful extensions: 502 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 23 Number of HSP's that attempted gapping in prelim test: 469 Number of HSP's gapped (non-prelim): 58 length of query: 150 length of database: 10,336,785 effective HSP length: 88 effective length of query: 62 effective length of database: 7,112,377 effective search space: 440967374 effective search space used: 440967374 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 54 (25.4 bits)