BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q62264 (150 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3ONT-A nrPDB 313 1e-86 1TQJ-A nrPDB 30 0.53 1S4O-A nrPDB 28 1.5 5ZWM-J nrPDB 26 6.0 1VRL-A nrPDB 25 9.1 >3ONT-A nrPDB Length = 152 Score = 313 bits (803), Expect = 1e-86, Method: Compositional matrix adjust. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MQVLTKRYPKNCLLTVMDRYSAVVRNMEQVVMIPSLLRDVQLSGPGGSVQDGAPDLYTYF 60 MQVLTKRYPKNCLLTVMDRYSAVVRNMEQVVMIPSLLRDVQLSGPGGSVQDGAPDLYTYF Sbjct: 3 MQVLTKRYPKNCLLTVMDRYSAVVRNMEQVVMIPSLLRDVQLSGPGGSVQDGAPDLYTYF 62 Query: 61 TMLKSICVEVDHGLLPREEWQAKVAGNETSEAENDAAETEEAEEDRISEELDLEAQFHLH 120 TMLKSICVEVDHGLLPREEWQAKVAGNETSEAENDAAETEEAEEDRISEELDLEAQFHLH Sbjct: 63 TMLKSICVEVDHGLLPREEWQAKVAGNETSEAENDAAETEEAEEDRISEELDLEAQFHLH 122 Query: 121 FCSLHHILTHLTRKAQEVTRKYQEMTGQVL 150 FCSLHHILTHLTRKAQEVTRKYQEMTGQVL Sbjct: 123 FCSLHHILTHLTRKAQEVTRKYQEMTGQVL 152 >1TQJ-A nrPDB Length = 230 Score = 29.6 bits (65), Expect = 0.53, Method: Compositional matrix adjust. Identities = 21/67 (31%), Positives = 27/67 (40%), Gaps = 15/67 (22%) Query: 45 PGGSVQDGAPDLYTYFTMLKSIC--------VEVDHGLLPREEWQ-------AKVAGNET 89 PG Q P++ L+ +C +EVD GL P WQ A VAG+ Sbjct: 145 PGFGGQSFIPEVLPKIRALRQMCDERGLDPWIEVDGGLKPNNTWQVLEAGANAIVAGSAV 204 Query: 90 SEAENDA 96 A N A Sbjct: 205 FNAPNYA 211 >1S4O-A nrPDB Length = 348 Score = 28.1 bits (61), Expect = 1.5, Method: Compositional matrix adjust. Identities = 12/30 (40%), Positives = 17/30 (56%) Query: 64 KSICVEVDHGLLPREEWQAKVAGNETSEAE 93 K++ EV G+LP+E W N+T AE Sbjct: 80 KAVSSEVKFGILPKEHWSYPEWINQTKAAE 109 >5ZWM-J nrPDB Length = 469 Score = 26.2 bits (56), Expect = 6.0, Method: Composition-based stats. Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 2/53 (3%) Query: 68 VEVDHGLLPREEWQAKVAGNETSEAENDAAETE-EAEEDRISEELDLEAQFHL 119 +++ GLLP+ E + K++ N S END T+ A E + +++DL + HL Sbjct: 258 IKIKLGLLPKPEPKVKLS-NMMSVFENDQNITDPTAWEKVVKDQVDLRKRKHL 309 >1VRL-A nrPDB Length = 369 Score = 25.4 bits (54), Expect = 9.1, Method: Compositional matrix adjust. Identities = 12/45 (26%), Positives = 19/45 (42%) Query: 88 ETSEAENDAAETEEAEEDRISEELDLEAQFHLHFCSLHHILTHLT 132 E E D A+ +E E + E+ L+ + S H +HL Sbjct: 270 EFPSCETDGADGKEKLEQMVGEQYGLQVELTEPIVSFEHAFSHLV 314 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3ONT-A nrPDB 317 1e-87 Sequences not found previously or not previously below threshold: 1TQJ-A nrPDB 29 1.1 1S4O-A nrPDB 29 1.2 5ZWM-J nrPDB 29 1.2 3PDX-A nrPDB 28 2.2 3DYD-A nrPDB 27 2.4 1VRL-A nrPDB 27 4.8 3PRB-A nrPDB 26 7.4 CONVERGED! >3ONT-A nrPDB Length = 152 Score = 317 bits (813), Expect = 1e-87, Method: Composition-based stats. Identities = 150/150 (100%), Positives = 150/150 (100%) Query: 1 MQVLTKRYPKNCLLTVMDRYSAVVRNMEQVVMIPSLLRDVQLSGPGGSVQDGAPDLYTYF 60 MQVLTKRYPKNCLLTVMDRYSAVVRNMEQVVMIPSLLRDVQLSGPGGSVQDGAPDLYTYF Sbjct: 3 MQVLTKRYPKNCLLTVMDRYSAVVRNMEQVVMIPSLLRDVQLSGPGGSVQDGAPDLYTYF 62 Query: 61 TMLKSICVEVDHGLLPREEWQAKVAGNETSEAENDAAETEEAEEDRISEELDLEAQFHLH 120 TMLKSICVEVDHGLLPREEWQAKVAGNETSEAENDAAETEEAEEDRISEELDLEAQFHLH Sbjct: 63 TMLKSICVEVDHGLLPREEWQAKVAGNETSEAENDAAETEEAEEDRISEELDLEAQFHLH 122 Query: 121 FCSLHHILTHLTRKAQEVTRKYQEMTGQVL 150 FCSLHHILTHLTRKAQEVTRKYQEMTGQVL Sbjct: 123 FCSLHHILTHLTRKAQEVTRKYQEMTGQVL 152 >1TQJ-A nrPDB Length = 230 Score = 28.5 bits (62), Expect = 1.1, Method: Composition-based stats. Identities = 21/67 (31%), Positives = 27/67 (40%), Gaps = 15/67 (22%) Query: 45 PGGSVQDGAPDLYTYFTMLKSIC--------VEVDHGLLPREEWQ-------AKVAGNET 89 PG Q P++ L+ +C +EVD GL P WQ A VAG+ Sbjct: 145 PGFGGQSFIPEVLPKIRALRQMCDERGLDPWIEVDGGLKPNNTWQVLEAGANAIVAGSAV 204 Query: 90 SEAENDA 96 A N A Sbjct: 205 FNAPNYA 211 >1S4O-A nrPDB Length = 348 Score = 28.5 bits (62), Expect = 1.2, Method: Composition-based stats. Identities = 15/36 (41%), Positives = 20/36 (55%), Gaps = 2/36 (5%) Query: 64 KSICVEVDHGLLPREEWQAKVAGNETSEAE--NDAA 97 K++ EV G+LP+E W N+T AE DAA Sbjct: 80 KAVSSEVKFGILPKEHWSYPEWINQTKAAEIRADAA 115 >5ZWM-J nrPDB Length = 469 Score = 28.5 bits (62), Expect = 1.2, Method: Composition-based stats. Identities = 18/53 (33%), Positives = 31/53 (58%), Gaps = 2/53 (3%) Query: 68 VEVDHGLLPREEWQAKVAGNETSEAENDAAETE-EAEEDRISEELDLEAQFHL 119 +++ GLLP+ E + K++ N S END T+ A E + +++DL + HL Sbjct: 258 IKIKLGLLPKPEPKVKLS-NMMSVFENDQNITDPTAWEKVVKDQVDLRKRKHL 309 >3PDX-A nrPDB Length = 402 Score = 27.8 bits (60), Expect = 2.2, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Query: 42 LSGPGGSVQDGAPDLYTYFTMLKSICVEVD-HGLLPREEWQ 81 L+ PG ++ P Y T+ +S+ +EV + LLP + W+ Sbjct: 115 LANPGQNILIPRPGFSLYRTLAESMGIEVKLYNLLPEKSWE 155 >3DYD-A nrPDB Length = 427 Score = 27.4 bits (59), Expect = 2.4, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 1/41 (2%) Query: 42 LSGPGGSVQDGAPDLYTYFTMLKSICVEVD-HGLLPREEWQ 81 L+ PG ++ P Y T+ +S+ +EV + LLP + W+ Sbjct: 138 LANPGQNILVPRPGFSLYKTLAESMGIEVKLYNLLPEKSWE 178 >1VRL-A nrPDB Length = 369 Score = 26.6 bits (57), Expect = 4.8, Method: Composition-based stats. Identities = 12/44 (27%), Positives = 19/44 (43%) Query: 88 ETSEAENDAAETEEAEEDRISEELDLEAQFHLHFCSLHHILTHL 131 E E D A+ +E E + E+ L+ + S H +HL Sbjct: 270 EFPSCETDGADGKEKLEQMVGEQYGLQVELTEPIVSFEHAFSHL 313 >3PRB-A nrPDB Length = 231 Score = 25.8 bits (55), Expect = 7.4, Method: Composition-based stats. Identities = 21/83 (25%), Positives = 41/83 (49%), Gaps = 2/83 (2%) Query: 16 VMDRYSAVVRNMEQVVMIPSLLRDVQLSGPGGSVQDGAPDLYTYFTMLKSICVEVDHGLL 75 V+D +V+ E V M L DV+++ G+V+ P+ + +++ + + + +L Sbjct: 148 VVDDKKNIVK--EIVKMYVPRLSDVKVTIRNGTVKIELPEFAPFIPNIQTAKMAIANEIL 205 Query: 76 PREEWQAKVAGNETSEAENDAAE 98 R E KV+ ET E + + E Sbjct: 206 KRLEDAEKVSFVETFERKKETKE 228 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.131 0.376 Lambda K H 0.267 0.0405 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,120,759 Number of Sequences: 36641 Number of extensions: 230783 Number of successful extensions: 605 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 17 Number of HSP's successfully gapped in prelim test: 13 Number of HSP's that attempted gapping in prelim test: 586 Number of HSP's gapped (non-prelim): 32 length of query: 150 length of database: 10,336,785 effective HSP length: 88 effective length of query: 62 effective length of database: 7,112,377 effective search space: 440967374 effective search space used: 440967374 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 54 (25.4 bits)