BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q61420 (336 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6OH3-A nrPDB 643 0.0 4I1I-A nrPDB 30 1.6 1BIP-A nrPDB 30 1.9 2G7U-A nrPDB 28 5.7 >6OH3-A nrPDB Length = 330 Score = 643 bits (1658), Expect = 0.0, Method: Compositional matrix adjust. Identities = 323/326 (99%), Positives = 323/326 (99%) Query: 1 MAPARENVSLFFKLYCLTVMTLVAAAYTVALRYTRTTAEELYFSTTAVCITEVIKLLISV 60 MAPARENVSLFFKLYCLTVMTLVAAAYTVALRYTRTTAEELYFSTTAVCITEVIKLLISV Sbjct: 1 MAPARENVSLFFKLYCLTVMTLVAAAYTVALRYTRTTAEELYFSTTAVCITEVIKLLISV 60 Query: 61 GLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQNNMAFLALSNLDAAVYQVT 120 GLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQNNMAFLALSNLDAAVYQVT Sbjct: 61 GLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQNNMAFLALSNLDAAVYQVT 120 Query: 121 YQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQATKVVVAQNPLLGFGA 180 YQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQATKVVVAQNPLLGFGA Sbjct: 121 YQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQATKVVVAQNPLLGFGA 180 Query: 181 IAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIVVTLAGTYLSDGAEIQEKGFFY 240 IAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIVVTLAGTYLSDGAEIQEKGFFY Sbjct: 181 IAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIVVTLAGTYLSDGAEIQEKGFFY 240 Query: 241 GYTYYVWFVIFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVLLFGLQITLSFA 300 GYTYYVWFVIFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVLLFGLQITLSFA Sbjct: 241 GYTYYVWFVIFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVLLFGLQITLSFA 300 Query: 301 LGALLVCVSIYLYGLPRQDTTSIQQE 326 LGALLVCVSIYLYGLPRQDTTS E Sbjct: 301 LGALLVCVSIYLYGLPRQDTTSNSLE 326 >4I1I-A nrPDB Length = 345 Score = 30.0 bits (66), Expect = 1.6, Method: Compositional matrix adjust. Identities = 14/38 (36%), Positives = 23/38 (60%), Gaps = 3/38 (7%) Query: 166 TKVVVAQNPLLGFGAIAIAVLCSGF---AGVYFEKVLK 200 KVVV +P + F +AIA++C F AG+ + +L+ Sbjct: 86 DKVVVTADPRVAFDGVAIAIMCGAFPRKAGMERKDLLE 123 >1BIP-A nrPDB Length = 122 Score = 29.6 bits (65), Expect = 1.9, Method: Compositional matrix adjust. Identities = 16/59 (27%), Positives = 33/59 (55%), Gaps = 5/59 (8%) Query: 47 AVCITEVIKLLISVGLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQNNMA 105 A C E +++L+ G++ S G+ + L +++ G P+++ + P LV V+ N+A Sbjct: 53 AYCRCEAVRILMD-GVVT----SSGQHEGRLLQDLPGCPRQVQRAFAPKLVTEVECNLA 106 >2G7U-A nrPDB Length = 257 Score = 28.1 bits (61), Expect = 5.7, Method: Compositional matrix adjust. Identities = 32/137 (23%), Positives = 57/137 (41%), Gaps = 15/137 (10%) Query: 53 VIKLLISVGLLAKETGSLGRFKASLSENVLG------SPKELAKLSVPSLVYAVQNNMAF 106 V ++L+++ L GS GR+ SL+ VL L + ++P L+ + Sbjct: 46 VRRILLTLQKLGYVAGSGGRW--SLTPRVLSIGQHYSESHALIEAAMPRLLEVAEKTQES 103 Query: 107 LALSNLDAAVYQVTYQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQAT 166 +L LD A V Y ++P + ++ +S + + G L+ W PA Sbjct: 104 ASLGVLDGA--DVVYAARVPVRRIMSI-----NVSVGTRVPAYATSMGRALLAWAPADVV 156 Query: 167 KVVVAQNPLLGFGAIAI 183 + VVA++ G I Sbjct: 157 ERVVAESTFQKLGPETI 173 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6OH3-A nrPDB 535 e-152 Sequences not found previously or not previously below threshold: 4I1I-A nrPDB 29 3.5 2G7U-A nrPDB 27 9.1 4M89-A nrPDB 27 9.5 CONVERGED! >6OH3-A nrPDB Length = 330 Score = 535 bits (1379), Expect = e-152, Method: Composition-based stats. Identities = 323/326 (99%), Positives = 323/326 (99%) Query: 1 MAPARENVSLFFKLYCLTVMTLVAAAYTVALRYTRTTAEELYFSTTAVCITEVIKLLISV 60 MAPARENVSLFFKLYCLTVMTLVAAAYTVALRYTRTTAEELYFSTTAVCITEVIKLLISV Sbjct: 1 MAPARENVSLFFKLYCLTVMTLVAAAYTVALRYTRTTAEELYFSTTAVCITEVIKLLISV 60 Query: 61 GLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQNNMAFLALSNLDAAVYQVT 120 GLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQNNMAFLALSNLDAAVYQVT Sbjct: 61 GLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQNNMAFLALSNLDAAVYQVT 120 Query: 121 YQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQATKVVVAQNPLLGFGA 180 YQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQATKVVVAQNPLLGFGA Sbjct: 121 YQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQATKVVVAQNPLLGFGA 180 Query: 181 IAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIVVTLAGTYLSDGAEIQEKGFFY 240 IAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIVVTLAGTYLSDGAEIQEKGFFY Sbjct: 181 IAIAVLCSGFAGVYFEKVLKSSDTSLWVRNIQMYLSGIVVTLAGTYLSDGAEIQEKGFFY 240 Query: 241 GYTYYVWFVIFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVLLFGLQITLSFA 300 GYTYYVWFVIFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVLLFGLQITLSFA Sbjct: 241 GYTYYVWFVIFLASVGGLYTSVVVKYTDNIMKGFSAAAAIVLSTIASVLLFGLQITLSFA 300 Query: 301 LGALLVCVSIYLYGLPRQDTTSIQQE 326 LGALLVCVSIYLYGLPRQDTTS E Sbjct: 301 LGALLVCVSIYLYGLPRQDTTSNSLE 326 >4I1I-A nrPDB Length = 345 Score = 28.8 bits (63), Expect = 3.5, Method: Composition-based stats. Identities = 11/24 (45%), Positives = 16/24 (66%) Query: 167 KVVVAQNPLLGFGAIAIAVLCSGF 190 KVVV +P + F +AIA++C F Sbjct: 87 KVVVTADPRVAFDGVAIAIMCGAF 110 >2G7U-A nrPDB Length = 257 Score = 27.2 bits (59), Expect = 9.1, Method: Composition-based stats. Identities = 32/138 (23%), Positives = 57/138 (41%), Gaps = 15/138 (10%) Query: 53 VIKLLISVGLLAKETGSLGRFKASLSENVLG------SPKELAKLSVPSLVYAVQNNMAF 106 V ++L+++ L GS GR+ SL+ VL L + ++P L+ + Sbjct: 46 VRRILLTLQKLGYVAGSGGRW--SLTPRVLSIGQHYSESHALIEAAMPRLLEVAEKTQES 103 Query: 107 LALSNLDAAVYQVTYQLKIPCTALCTVLMLNRTLSKLQWISVFMLCGGVTLVQWKPAQAT 166 +L LD A V Y ++P + ++ +S + + G L+ W PA Sbjct: 104 ASLGVLDGA--DVVYAARVPVRRIMSI-----NVSVGTRVPAYATSMGRALLAWAPADVV 156 Query: 167 KVVVAQNPLLGFGAIAIA 184 + VVA++ G I Sbjct: 157 ERVVAESTFQKLGPETIG 174 >4M89-A nrPDB Length = 261 Score = 27.2 bits (59), Expect = 9.5, Method: Composition-based stats. Identities = 22/64 (34%), Positives = 32/64 (50%), Gaps = 6/64 (9%) Query: 56 LLISVGLLAKETGSLGRFKASLSENVLGSPKELAKLSVPSLVYAVQ-----NNMAFLALS 110 L+ S+G KE S G F S+S + E++ S+P+L A + N A +ALS Sbjct: 88 LVHSIGFAPKEALS-GDFLDSISREAFNTAHEISAYSLPALAKAARPMMRGRNSAIVALS 146 Query: 111 NLDA 114 L A Sbjct: 147 YLGA 150 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.324 0.144 0.436 Lambda K H 0.267 0.0450 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 14,341,342 Number of Sequences: 36641 Number of extensions: 623836 Number of successful extensions: 1505 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 14 Number of HSP's that attempted gapping in prelim test: 1500 Number of HSP's gapped (non-prelim): 19 length of query: 336 length of database: 10,336,785 effective HSP length: 98 effective length of query: 238 effective length of database: 6,745,967 effective search space: 1605540146 effective search space used: 1605540146 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.5 bits) S2: 60 (27.6 bits)