BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q5CZR5 (315 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4ZNQ-A nrPDB 642 0.0 1II2-A nrPDB 32 0.47 5F9Y-A nrPDB 30 1.3 3HQT-A nrPDB 29 2.9 5XIP-A nrPDB 28 4.3 1GM5-A nrPDB 27 8.4 1NL3-A nrPDB 27 8.5 >4ZNQ-A nrPDB Length = 335 Score = 642 bits (1655), Expect = 0.0, Method: Compositional matrix adjust. Identities = 315/315 (100%), Positives = 315/315 (100%) Query: 1 MTYPTNLEIIGGQGGSSFSFTGENNGASLEKIWVWVGGWQIKAVRAWLSDGRDETFGVPS 60 MTYPTNLEIIGGQGGSSFSFTGENNGASLEKIWVWVGGWQIKAVRAWLSDGRDETFGVPS Sbjct: 21 MTYPTNLEIIGGQGGSSFSFTGENNGASLEKIWVWVGGWQIKAVRAWLSDGRDETFGVPS 80 Query: 61 GSHQEYVFTPGECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSWGLKTEYPMDVGS 120 GSHQEYVFTPGECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSWGLKTEYPMDVGS Sbjct: 81 GSHQEYVFTPGECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSWGLKTEYPMDVGS 140 Query: 121 GYCLGIVGRGGSDIDCMGFMFLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTS 180 GYCLGIVGRGGSDIDCMGFMFLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTS Sbjct: 141 GYCLGIVGRGGSDIDCMGFMFLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTS 200 Query: 181 VKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVSTGFSISFGVESTHSLE 240 VKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVSTGFSISFGVESTHSLE Sbjct: 201 VKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVSTGFSISFGVESTHSLE 260 Query: 241 QTDEKNETLTTTVEVPPKKKVDVHITIGRASFDLPYTGTVKITCKNGSVLQYETKGQYKG 300 QTDEKNETLTTTVEVPPKKKVDVHITIGRASFDLPYTGTVKITCKNGSVLQYETKGQYKG Sbjct: 261 QTDEKNETLTTTVEVPPKKKVDVHITIGRASFDLPYTGTVKITCKNGSVLQYETKGQYKG 320 Query: 301 VAYTDIKVNTVEKDL 315 VAYTDIKVNTVEKDL Sbjct: 321 VAYTDIKVNTVEKDL 335 >1II2-A nrPDB Length = 524 Score = 31.6 bits (70), Expect = 0.47, Method: Compositional matrix adjust. Identities = 35/153 (22%), Positives = 58/153 (37%), Gaps = 13/153 (8%) Query: 85 GTRLGAIKFKTNKGGEFFA-HMTSWGLKTEYPMD--------VGSGYCLGIVGRGGSDID 135 GTR F + GG F H T +G + M + +GY G RG + Sbjct: 370 GTRTARPIFSSCFGGPFLVRHATFYGEQLAEKMQKHNSRVWLLNTGYAGGRADRGAKRMP 429 Query: 136 C-MGFMFLNAVQSTVLTNVNY---PTINQLIPKVATEEIKSVSFENKTSVKQEQKVETSK 191 + ++A+ L Y P IPK + + + K Q ETSK Sbjct: 430 LRVTRAIIDAIHDGTLDRTEYEEYPGWGLHIPKYVAKVPEHLLNPRKAWKDVRQFNETSK 489 Query: 192 KVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVS 224 +++ S + F++ S E+ + +P E + Sbjct: 490 ELVAMFQESFSARFAAKASQEMKSAVPRYVEFA 522 >5F9Y-A nrPDB Length = 511 Score = 30.0 bits (66), Expect = 1.3, Method: Compositional matrix adjust. Identities = 11/27 (40%), Positives = 19/27 (70%) Query: 86 TRLGAIKFKTNKGGEFFAHMTSWGLKT 112 +++ ++F+ KG + +AH TSWGL T Sbjct: 251 SKMFGVEFEDEKGNKEYAHQTSWGLTT 277 >3HQT-A nrPDB Length = 409 Score = 28.9 bits (63), Expect = 2.9, Method: Compositional matrix adjust. Identities = 14/37 (37%), Positives = 17/37 (45%) Query: 72 ECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSW 108 EC S S W G + TN +FFAHM+ W Sbjct: 121 ECLLSQSGWNANVGLLQTICQPNTNVYIDFFAHMSLW 157 >5XIP-A nrPDB Length = 498 Score = 28.5 bits (62), Expect = 4.3, Method: Compositional matrix adjust. Identities = 11/27 (40%), Positives = 17/27 (62%) Query: 86 TRLGAIKFKTNKGGEFFAHMTSWGLKT 112 +R+ +I+F+ KG + H TSWG T Sbjct: 238 SRMFSIEFEDEKGAKQLVHQTSWGCTT 264 >1GM5-A nrPDB Length = 780 Score = 27.3 bits (59), Expect = 8.4, Method: Compositional matrix adjust. Identities = 29/128 (22%), Positives = 52/128 (40%), Gaps = 6/128 (4%) Query: 141 FLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTS-----VKQEQKVETSKKVIK 195 FLN V+ + VN I+QL+ ++ +++ E K VK+ + ++K + Sbjct: 25 FLNEVEKMLKNQVNTRRIHQLLKELDDPLLENKDLEEKLQAFLDYVKEIPNLPEARKRYR 84 Query: 196 TS-SWSMTKSFSSTFSVEVSAGIPEIAEVSTGFSISFGVESTHSLEQTDEKNETLTTTVE 254 S M + S F ++ E ++ST + GV + ETL +E Sbjct: 85 IQKSLEMIEKLRSWFLIDYLECSGEEVDLSTDIQYAKGVGPNRKKKLKKLGIETLRDLLE 144 Query: 255 VPPKKKVD 262 P+ D Sbjct: 145 FFPRDYED 152 >1NL3-A nrPDB Length = 922 Score = 27.3 bits (59), Expect = 8.5, Method: Compositional matrix adjust. Identities = 21/61 (34%), Positives = 29/61 (47%), Gaps = 5/61 (8%) Query: 135 DCMGFMFLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTSVKQEQKVETSKKVI 194 D + F A T+LT +N P + K+ T IKS +T V+Q Q E K V+ Sbjct: 618 DELMRRFNGAALETLLTRLNLPDDVPIEAKMVTRAIKSA----QTQVEQ-QNFEVRKNVL 672 Query: 195 K 195 K Sbjct: 673 K 673 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4ZNQ-A nrPDB 668 0.0 Sequences not found previously or not previously below threshold: 5GAQ-A nrPDB 34 0.11 5F9Y-A nrPDB 32 0.29 1II2-A nrPDB 32 0.47 5XIP-A nrPDB 30 1.4 3UCF-A nrPDB 29 2.5 3HQT-A nrPDB 29 2.7 2OXV-A nrPDB 29 3.4 CONVERGED! >4ZNQ-A nrPDB Length = 335 Score = 668 bits (1723), Expect = 0.0, Method: Composition-based stats. Identities = 315/315 (100%), Positives = 315/315 (100%) Query: 1 MTYPTNLEIIGGQGGSSFSFTGENNGASLEKIWVWVGGWQIKAVRAWLSDGRDETFGVPS 60 MTYPTNLEIIGGQGGSSFSFTGENNGASLEKIWVWVGGWQIKAVRAWLSDGRDETFGVPS Sbjct: 21 MTYPTNLEIIGGQGGSSFSFTGENNGASLEKIWVWVGGWQIKAVRAWLSDGRDETFGVPS 80 Query: 61 GSHQEYVFTPGECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSWGLKTEYPMDVGS 120 GSHQEYVFTPGECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSWGLKTEYPMDVGS Sbjct: 81 GSHQEYVFTPGECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSWGLKTEYPMDVGS 140 Query: 121 GYCLGIVGRGGSDIDCMGFMFLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTS 180 GYCLGIVGRGGSDIDCMGFMFLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTS Sbjct: 141 GYCLGIVGRGGSDIDCMGFMFLNAVQSTVLTNVNYPTINQLIPKVATEEIKSVSFENKTS 200 Query: 181 VKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVSTGFSISFGVESTHSLE 240 VKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVSTGFSISFGVESTHSLE Sbjct: 201 VKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVSTGFSISFGVESTHSLE 260 Query: 241 QTDEKNETLTTTVEVPPKKKVDVHITIGRASFDLPYTGTVKITCKNGSVLQYETKGQYKG 300 QTDEKNETLTTTVEVPPKKKVDVHITIGRASFDLPYTGTVKITCKNGSVLQYETKGQYKG Sbjct: 261 QTDEKNETLTTTVEVPPKKKVDVHITIGRASFDLPYTGTVKITCKNGSVLQYETKGQYKG 320 Query: 301 VAYTDIKVNTVEKDL 315 VAYTDIKVNTVEKDL Sbjct: 321 VAYTDIKVNTVEKDL 335 >5GAQ-A nrPDB Length = 310 Score = 33.5 bits (75), Expect = 0.11, Method: Composition-based stats. Identities = 23/88 (26%), Positives = 45/88 (51%), Gaps = 3/88 (3%) Query: 175 FENKTSVKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGIP-EIAEVSTGFSISFGV 233 +EN+ S +QK+ +K + +S + T + + + +S G EI V +S S Sbjct: 26 YENRGSTSVDQKITITKGMKNVNSETRTVTATHSIGSTISTGDAFEIGSVEVSYSHSHE- 84 Query: 234 ESTHSLEQTD-EKNETLTTTVEVPPKKK 260 ES S+ +T+ +++ + T+ +PP K Sbjct: 85 ESQVSMTETEVYESKVIEHTITIPPTSK 112 >5F9Y-A nrPDB Length = 511 Score = 32.4 bits (72), Expect = 0.29, Method: Composition-based stats. Identities = 11/27 (40%), Positives = 19/27 (70%) Query: 86 TRLGAIKFKTNKGGEFFAHMTSWGLKT 112 +++ ++F+ KG + +AH TSWGL T Sbjct: 251 SKMFGVEFEDEKGNKEYAHQTSWGLTT 277 >1II2-A nrPDB Length = 524 Score = 31.6 bits (70), Expect = 0.47, Method: Composition-based stats. Identities = 35/155 (22%), Positives = 58/155 (37%), Gaps = 13/155 (8%) Query: 83 GAGTRLGAIKFKTNKGGEFFA-HMTSWGLKTEYPMD--------VGSGYCLGIVGRGGSD 133 GTR F + GG F H T +G + M + +GY G RG Sbjct: 368 AGGTRTARPIFSSCFGGPFLVRHATFYGEQLAEKMQKHNSRVWLLNTGYAGGRADRGAKR 427 Query: 134 IDC-MGFMFLNAVQSTVLTNVNY---PTINQLIPKVATEEIKSVSFENKTSVKQEQKVET 189 + + ++A+ L Y P IPK + + + K Q ET Sbjct: 428 MPLRVTRAIIDAIHDGTLDRTEYEEYPGWGLHIPKYVAKVPEHLLNPRKAWKDVRQFNET 487 Query: 190 SKKVIKTSSWSMTKSFSSTFSVEVSAGIPEIAEVS 224 SK+++ S + F++ S E+ + +P E + Sbjct: 488 SKELVAMFQESFSARFAAKASQEMKSAVPRYVEFA 522 >5XIP-A nrPDB Length = 498 Score = 30.1 bits (66), Expect = 1.4, Method: Composition-based stats. Identities = 11/27 (40%), Positives = 17/27 (62%) Query: 86 TRLGAIKFKTNKGGEFFAHMTSWGLKT 112 +R+ +I+F+ KG + H TSWG T Sbjct: 238 SRMFSIEFEDEKGAKQLVHQTSWGCTT 264 >3UCF-A nrPDB Length = 223 Score = 29.3 bits (64), Expect = 2.5, Method: Composition-based stats. Identities = 22/79 (27%), Positives = 33/79 (41%), Gaps = 2/79 (2%) Query: 211 VEVSAGIPEIAEVSTGFSISFGVESTHSLEQTDEKNETLTTTVEVPPKKKV-DVHITIGR 269 +E I +A TG IS H E + + T P KV DV +T + Sbjct: 26 LESEHTIVHVASRQTGLDISDEKSVYHYFETIGAFDHLIVTAGSYAPAGKVVDVEVTQAK 85 Query: 270 ASFDLPYTGTVKITCKNGS 288 +FD + G V + K+G+ Sbjct: 86 YAFDTKFWGAV-LAAKHGA 103 >3HQT-A nrPDB Length = 409 Score = 28.9 bits (63), Expect = 2.7, Method: Composition-based stats. Identities = 14/37 (37%), Positives = 17/37 (45%) Query: 72 ECFTSLSLWGNGAGTRLGAIKFKTNKGGEFFAHMTSW 108 EC S S W G + TN +FFAHM+ W Sbjct: 121 ECLLSQSGWNANVGLLQTICQPNTNVYIDFFAHMSLW 157 >2OXV-A nrPDB Length = 277 Score = 28.5 bits (62), Expect = 3.4, Method: Composition-based stats. Identities = 18/80 (22%), Positives = 38/80 (47%), Gaps = 3/80 (3%) Query: 158 INQLIPKVATEEIKSVSFENKTSVKQEQKVETSKKVIKTSSWSMTKSFSSTFSVEVSAGI 217 +++L+ K + E +SF + S+K+ + E KK+ ++ F S S++ GI Sbjct: 38 VSKLVKKALSNEYPQLSFRYRDSIKKTEINEALKKIDPDLGGTL---FVSNSSIKPDGGI 94 Query: 218 PEIAEVSTGFSISFGVESTH 237 E+ + + + E+ H Sbjct: 95 VEVKDDYGEWRVVLVAEAKH 114 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.131 0.386 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,841,005 Number of Sequences: 36641 Number of extensions: 538898 Number of successful extensions: 1010 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 9 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 999 Number of HSP's gapped (non-prelim): 26 length of query: 315 length of database: 10,336,785 effective HSP length: 97 effective length of query: 218 effective length of database: 6,782,608 effective search space: 1478608544 effective search space used: 1478608544 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 59 (27.3 bits)