BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q53TN4 (286 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5ZLG-A nrPDB 578 e-165 4O6Y-A nrPDB 120 5e-28 1HMW-A nrPDB 35 0.027 1ZAU-A nrPDB 31 0.65 3SGI-A nrPDB 31 0.78 5M5W-M nrPDB 28 4.4 6H7W-Q nrPDB 28 6.0 3NFI-A nrPDB 27 7.7 >5ZLG-A nrPDB Length = 292 Score = 578 bits (1489), Expect = e-165, Method: Compositional matrix adjust. Identities = 286/286 (100%), Positives = 286/286 (100%) Query: 1 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF 60 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF Sbjct: 1 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF 60 Query: 61 IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH 120 IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH Sbjct: 61 IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH 120 Query: 121 SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE 180 SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE Sbjct: 121 SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE 180 Query: 181 KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG 240 KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG Sbjct: 181 KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG 240 Query: 241 TEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM 286 TEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM Sbjct: 241 TEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM 286 >4O6Y-A nrPDB Length = 230 Score = 120 bits (302), Expect = 5e-28, Method: Compositional matrix adjust. Identities = 74/207 (35%), Positives = 109/207 (52%), Gaps = 5/207 (2%) Query: 18 LVGFLSVIFALVWVLHYREGLGW--DGSALEFNWHPVLMVTGFVFIQGIAIIVYRLPWTW 75 ++GF+ L W +HYR GL D FN HPV+MV G + G A++ Y+ + Sbjct: 17 VLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLAYK---SV 73 Query: 76 KCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLHSWVGLIAVICYLLQL 135 + +K L K +H L A IL++I V A + H I N YSLHSW+GL + + Q Sbjct: 74 QGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFAFQW 133 Query: 136 LSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIFSLRDPAYSTFP 195 +GF + P + RA LMP HV+ GI I+ + TA G+ EK+ F + + + Sbjct: 134 AAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVITRYS 193 Query: 196 PEGVFVNTLGLLILVFGALIFWIVTRP 222 E + VNT+G+LIL+ G + V P Sbjct: 194 TEAMLVNTMGVLILILGGFVILGVVTP 220 >1HMW-A nrPDB Length = 700 Score = 35.4 bits (80), Expect = 0.027, Method: Compositional matrix adjust. Identities = 28/85 (32%), Positives = 41/85 (48%), Gaps = 7/85 (8%) Query: 1 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVT--GF 58 +A+ ++R L ALL + +F V +HY EGL +D S L+ P L ++ G Sbjct: 179 IALHYFYRALLTSDEALLSFAVKELFYPVQFVHYEEGLQYDYSYLQHG--PQLQISSYGA 236 Query: 59 VFIQGI---AIIVYRLPWTWKCSKL 80 VFI G+ A V P+ KL Sbjct: 237 VFITGVLKLANYVRDTPYALSTEKL 261 >1ZAU-A nrPDB Length = 328 Score = 30.8 bits (68), Expect = 0.65, Method: Compositional matrix adjust. Identities = 18/59 (30%), Positives = 32/59 (54%), Gaps = 7/59 (11%) Query: 126 IAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIF 184 + +IC+ L + GF L A L+LRA+ +P+ + T +AT L G+ E++ + Sbjct: 231 LRMICHGLGHVEGFRPATLHQAYLALRAWGLPVSEH-------TTLATDLAGVRERIDY 282 >3SGI-A nrPDB Length = 615 Score = 30.8 bits (68), Expect = 0.78, Method: Compositional matrix adjust. Identities = 18/59 (30%), Positives = 32/59 (54%), Gaps = 7/59 (11%) Query: 126 IAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIF 184 + +IC+ L + GF L A L+LRA+ +P+ + T +AT L G+ E++ + Sbjct: 231 LRMICHGLGHVEGFRPATLHQAYLALRAWGLPVSEH-------TTLATDLAGVRERIDY 282 >5M5W-M nrPDB Length = 415 Score = 28.1 bits (61), Expect = 4.4, Method: Compositional matrix adjust. Identities = 13/53 (24%), Positives = 25/53 (47%) Query: 128 VICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE 180 ++CY+L ++ F++ PL+ L P V S + G ++ A + E Sbjct: 328 ILCYILAIIMHLDNFIVEITPLAHELNLKPSKVVSLFRVLGAIVKGATVAQAE 380 >6H7W-Q nrPDB Length = 846 Score = 27.7 bits (60), Expect = 6.0, Method: Compositional matrix adjust. Identities = 12/28 (42%), Positives = 18/28 (64%) Query: 106 ENHNVNNIANMYSLHSWVGLIAVICYLL 133 ENH VN++A++Y L + G I YL+ Sbjct: 71 ENHPVNHLADLYELVQYAGNIIPRLYLM 98 >3NFI-A nrPDB Length = 237 Score = 27.3 bits (59), Expect = 7.7, Method: Compositional matrix adjust. Identities = 13/54 (24%), Positives = 25/54 (46%) Query: 127 AVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE 180 ++CY+L ++ F++ PL+ L P V S + G ++ A + E Sbjct: 161 KILCYILAIIMHLDNFIVEITPLAHELNLKPSKVVSLFRVLGAIVKGATVAQAE 214 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5ZLG-A nrPDB 456 e-129 4O6Y-A nrPDB 283 9e-77 Sequences not found previously or not previously below threshold: 1HMW-A nrPDB 27 9.1 CONVERGED! >5ZLG-A nrPDB Length = 292 Score = 456 bits (1174), Expect = e-129, Method: Composition-based stats. Identities = 286/286 (100%), Positives = 286/286 (100%) Query: 1 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF 60 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF Sbjct: 1 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF 60 Query: 61 IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH 120 IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH Sbjct: 61 IQGIAIIVYRLPWTWKCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLH 120 Query: 121 SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE 180 SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE Sbjct: 121 SWVGLIAVICYLLQLLSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTE 180 Query: 181 KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG 240 KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG Sbjct: 181 KLIFSLRDPAYSTFPPEGVFVNTLGLLILVFGALIFWIVTRPQWKRPKEPNSTILHPNGG 240 Query: 241 TEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM 286 TEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM Sbjct: 241 TEQGARGSMPAYSGNNMDKSDSELNSEVAARKRNLALDEAGQRSTM 286 >4O6Y-A nrPDB Length = 230 Score = 283 bits (723), Expect = 9e-77, Method: Composition-based stats. Identities = 74/207 (35%), Positives = 109/207 (52%), Gaps = 5/207 (2%) Query: 18 LVGFLSVIFALVWVLHYREGLGW--DGSALEFNWHPVLMVTGFVFIQGIAIIVYRLPWTW 75 ++GF+ L W +HYR GL D FN HPV+MV G + G A++ Y+ + Sbjct: 17 VLGFIIAALVLTWTVHYRGGLALSSDNKDHIFNVHPVMMVIGLILFNGEAMLAYK---SV 73 Query: 76 KCSKLLMKSIHAGLNAVAAILAIISVVAVFENHNVNNIANMYSLHSWVGLIAVICYLLQL 135 + +K L K +H L A IL++I V A + H I N YSLHSW+GL + + Q Sbjct: 74 QGTKNLKKLVHLTLQLTAFILSLIGVWAALKFHIDKGIENFYSLHSWLGLACLFLFAFQW 133 Query: 136 LSGFSVFLLPWAPLSLRAFLMPIHVYSGIVIFGTVIATALMGLTEKLIFSLRDPAYSTFP 195 +GF + P + RA LMP HV+ GI I+ + TA G+ EK+ F + + + Sbjct: 134 AAGFVTYWYPGGSRNSRASLMPWHVFLGISIYALALVTATTGILEKVTFLQVNQVITRYS 193 Query: 196 PEGVFVNTLGLLILVFGALIFWIVTRP 222 E + VNT+G+LIL+ G + V P Sbjct: 194 TEAMLVNTMGVLILILGGFVILGVVTP 220 >1HMW-A nrPDB Length = 700 Score = 27.3 bits (59), Expect = 9.1, Method: Composition-based stats. Identities = 21/68 (30%), Positives = 33/68 (48%) Query: 1 MAMEGYWRFLALLGSALLVGFLSVIFALVWVLHYREGLGWDGSALEFNWHPVLMVTGFVF 60 +A+ ++R L ALL + +F V +HY EGL +D S L+ + G VF Sbjct: 179 IALHYFYRALLTSDEALLSFAVKELFYPVQFVHYEEGLQYDYSYLQHGPQLQISSYGAVF 238 Query: 61 IQGIAIIV 68 I G+ + Sbjct: 239 ITGVLKLA 246 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.327 0.140 0.424 Lambda K H 0.267 0.0428 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,039,862 Number of Sequences: 36641 Number of extensions: 506719 Number of successful extensions: 1346 Number of sequences better than 10.0: 32 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 36 Number of HSP's that attempted gapping in prelim test: 1318 Number of HSP's gapped (non-prelim): 58 length of query: 286 length of database: 10,336,785 effective HSP length: 96 effective length of query: 190 effective length of database: 6,819,249 effective search space: 1295657310 effective search space used: 1295657310 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.7 bits) S2: 59 (27.3 bits)