BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q53FT3 (197 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3WVZ-A nrPDB 405 e-114 5JRH-A nrPDB 28 2.6 1YHP-A nrPDB 27 4.7 2RGH-A nrPDB 27 5.7 3EH2-A nrPDB 27 7.5 >3WVZ-A nrPDB Length = 201 Score = 405 bits (1041), Expect = e-114, Method: Compositional matrix adjust. Identities = 197/197 (100%), Positives = 197/197 (100%) Query: 1 MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPD 60 MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPD Sbjct: 5 MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPD 64 Query: 61 SNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDS 120 SNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDS Sbjct: 65 SNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDS 124 Query: 121 MAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKW 180 MAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKW Sbjct: 125 MAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKW 184 Query: 181 YENFQRRLAQNPLFWKT 197 YENFQRRLAQNPLFWKT Sbjct: 185 YENFQRRLAQNPLFWKT 201 >5JRH-A nrPDB Length = 660 Score = 28.1 bits (61), Expect = 2.6, Method: Composition-based stats. Identities = 12/23 (52%), Positives = 14/23 (60%) Query: 171 FIPANVVLKWYENFQRRLAQNPL 193 F P NV +KWYE+ LA N L Sbjct: 60 FAPGNVSIKWYEDGTLNLAANCL 82 >1YHP-A nrPDB Length = 212 Score = 27.3 bits (59), Expect = 4.7, Method: Compositional matrix adjust. Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Query: 28 PDYESINHVVVFMLGTIPFPEGMGGSVYFSYPDSNG 63 PD E ++H+ V T P+ + GSVYF Y + G Sbjct: 145 PDSEIVSHLTVRQTHT-PYDYVVNGSVYFKYSPTTG 179 >2RGH-A nrPDB Length = 571 Score = 26.9 bits (58), Expect = 5.7, Method: Compositional matrix adjust. Identities = 17/69 (24%), Positives = 30/69 (43%) Query: 61 SNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDS 120 ++G P G +P + +SG K + + GA+ ++R + GI + +DS Sbjct: 366 NSGSPSTISRGSSLEREPDGLLTLSGGKITDYRKMAEGALRLIRQLLKEEYGIETKEIDS 425 Query: 121 MAQQTPVGN 129 Q GN Sbjct: 426 KKYQISGGN 434 >3EH2-A nrPDB Length = 766 Score = 26.6 bits (57), Expect = 7.5, Method: Composition-based stats. Identities = 20/75 (26%), Positives = 31/75 (41%), Gaps = 3/75 (4%) Query: 99 AMNIVRTPSVAQIGISVELLDSMAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAV 158 + N +RT + + E L S+ P A S T + +FYN SS A Sbjct: 183 SYNAIRT---GLVRLLCEELKSLLDFLPREGGAEESAIRVGFVTYNKVLHFYNVKSSLAQ 239 Query: 159 SQAQMTPSPSEMFIP 173 Q + ++MF+P Sbjct: 240 PQMMVVSDVADMFVP 254 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3WVZ-A nrPDB 405 e-114 Sequences not found previously or not previously below threshold: 1YHP-A nrPDB 28 2.2 5JRH-A nrPDB 28 2.4 4AY7-A nrPDB 27 5.0 2X5N-A nrPDB 27 6.1 3EH2-A nrPDB 27 6.5 3T6J-A nrPDB 26 9.5 CONVERGED! >3WVZ-A nrPDB Length = 201 Score = 405 bits (1042), Expect = e-114, Method: Composition-based stats. Identities = 197/197 (100%), Positives = 197/197 (100%) Query: 1 MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPD 60 MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPD Sbjct: 5 MFGCLVAGRLVQTAAQQVAEDKFVFDLPDYESINHVVVFMLGTIPFPEGMGGSVYFSYPD 64 Query: 61 SNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDS 120 SNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDS Sbjct: 65 SNGMPVWQLLGFVTNGKPSAIFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDS 124 Query: 121 MAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKW 180 MAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKW Sbjct: 125 MAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAVSQAQMTPSPSEMFIPANVVLKW 184 Query: 181 YENFQRRLAQNPLFWKT 197 YENFQRRLAQNPLFWKT Sbjct: 185 YENFQRRLAQNPLFWKT 201 >1YHP-A nrPDB Length = 212 Score = 28.5 bits (62), Expect = 2.2, Method: Composition-based stats. Identities = 14/36 (38%), Positives = 20/36 (55%), Gaps = 1/36 (2%) Query: 28 PDYESINHVVVFMLGTIPFPEGMGGSVYFSYPDSNG 63 PD E ++H+ V T P+ + GSVYF Y + G Sbjct: 145 PDSEIVSHLTVRQTHT-PYDYVVNGSVYFKYSPTTG 179 >5JRH-A nrPDB Length = 660 Score = 28.1 bits (61), Expect = 2.4, Method: Composition-based stats. Identities = 12/23 (52%), Positives = 14/23 (60%) Query: 171 FIPANVVLKWYENFQRRLAQNPL 193 F P NV +KWYE+ LA N L Sbjct: 60 FAPGNVSIKWYEDGTLNLAANCL 82 >4AY7-A nrPDB Length = 348 Score = 26.9 bits (58), Expect = 5.0, Method: Composition-based stats. Identities = 19/61 (31%), Positives = 28/61 (45%), Gaps = 1/61 (1%) Query: 99 AMNIVRTPSVAQIGISVEL-LDSMAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFA 157 A++I S+ VE D +A PV + + S DSF QF + L F + +S Sbjct: 184 ALDIATEASIIYANAMVEAGADVIAIADPVASPDLMSPDSFRQFLKSRLQKFASSVNSVT 243 Query: 158 V 158 V Sbjct: 244 V 244 >2X5N-A nrPDB Length = 192 Score = 26.9 bits (58), Expect = 6.1, Method: Composition-based stats. Identities = 15/53 (28%), Positives = 24/53 (45%) Query: 81 IFKISGLKSGEGSQHPFGAMNIVRTPSVAQIGISVELLDSMAQQTPVGNAAVS 133 I I L++ QH A N + + I S +LL + Q+P+G V+ Sbjct: 140 IIHIGELQNESALQHFIDAANSSDSCHLVSIPPSPQLLSDLVNQSPIGQGVVA 192 >3EH2-A nrPDB Length = 766 Score = 26.6 bits (57), Expect = 6.5, Method: Composition-based stats. Identities = 20/75 (26%), Positives = 31/75 (41%), Gaps = 3/75 (4%) Query: 99 AMNIVRTPSVAQIGISVELLDSMAQQTPVGNAAVSSVDSFTQFTQKMLDNFYNFASSFAV 158 + N +RT + + E L S+ P A S T + +FYN SS A Sbjct: 183 SYNAIRT---GLVRLLCEELKSLLDFLPREGGAEESAIRVGFVTYNKVLHFYNVKSSLAQ 239 Query: 159 SQAQMTPSPSEMFIP 173 Q + ++MF+P Sbjct: 240 PQMMVVSDVADMFVP 254 >3T6J-A nrPDB Length = 726 Score = 26.2 bits (56), Expect = 9.5, Method: Composition-based stats. Identities = 23/87 (26%), Positives = 37/87 (42%), Gaps = 2/87 (2%) Query: 56 FSYPDSNGMPVWQL-LGFVTNGKPSAIFKI-SGLKSGEGSQHPFGAMNIVRTPSVAQIGI 113 F D G P +++ L V +PS ++ S LKS E PF P + ++ Sbjct: 201 FKEVDGEGKPYYEVRLASVLGSEPSLDSEVTSKLKSYEFRGSPFQVTRGDYAPILQKVVE 260 Query: 114 SVELLDSMAQQTPVGNAAVSSVDSFTQ 140 +E + A + G ++SFTQ Sbjct: 261 QLEKAKAYAANSHQGQMLAQYIESFTQ 287 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.134 0.403 Lambda K H 0.267 0.0418 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,133,424 Number of Sequences: 36641 Number of extensions: 331498 Number of successful extensions: 777 Number of sequences better than 10.0: 8 Number of HSP's better than 10.0 without gapping: 10 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 767 Number of HSP's gapped (non-prelim): 18 length of query: 197 length of database: 10,336,785 effective HSP length: 92 effective length of query: 105 effective length of database: 6,965,813 effective search space: 731410365 effective search space used: 731410365 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 56 (26.2 bits)