BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q47150 (86 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4Q2U-A nrPDB 171 6e-44 4FXE-A nrPDB 33 0.028 3FEW-X nrPDB 28 1.1 2MV2-A nrPDB 27 2.6 2V6B-A nrPDB 26 3.9 3WGI-A nrPDB 25 7.7 1BG3-A nrPDB 25 8.6 1GG3-A nrPDB 25 8.8 3ZZN-A nrPDB 25 9.3 >4Q2U-A nrPDB Length = 86 Score = 171 bits (433), Expect = 6e-44, Method: Compositional matrix adjust. Identities = 86/86 (100%), Positives = 86/86 (100%) Query: 1 MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQ 60 MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQ Sbjct: 1 MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQ 60 Query: 61 SIKNSEAGIDVHKAKDADDLFDKLGI 86 SIKNSEAGIDVHKAKDADDLFDKLGI Sbjct: 61 SIKNSEAGIDVHKAKDADDLFDKLGI 86 >4FXE-A nrPDB Length = 79 Score = 33.1 bits (74), Expect = 0.028, Method: Compositional matrix adjust. Identities = 16/40 (40%), Positives = 26/40 (65%) Query: 10 RIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPF 49 RID++LK ++ L MG+T S+ +R+ L +A + LPF Sbjct: 7 RIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPF 46 >3FEW-X nrPDB Length = 505 Score = 27.7 bits (60), Expect = 1.1, Method: Compositional matrix adjust. Identities = 26/98 (26%), Positives = 46/98 (46%), Gaps = 22/98 (22%) Query: 8 RARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEA 67 R RI D+ N+AA+V++ +G + D + +ARE A D++ TI+S ++ A Sbjct: 299 RDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIAD--DVKNFQGKTIRSYDDAMA 356 Query: 68 GI--------------------DVHKAKDADDLFDKLG 85 + +V ++ DA D+ +KLG Sbjct: 357 SLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLG 394 >2MV2-A nrPDB Length = 148 Score = 26.6 bits (57), Expect = 2.6, Method: Compositional matrix adjust. Identities = 11/47 (23%), Positives = 28/47 (59%), Gaps = 3/47 (6%) Query: 27 GLTISDLVRITLTKVAREK---ALPFDLREPNQLTIQSIKNSEAGID 70 G+T+SD+ + T ++ ++K + F +R+ Q+ ++++ + A D Sbjct: 4 GVTVSDVCKTTYEEIKKDKKHRYVIFYIRDEKQIDVETVADRNAEYD 50 >2V6B-A nrPDB Length = 304 Score = 25.8 bits (55), Expect = 3.9, Method: Compositional matrix adjust. Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 4/38 (10%) Query: 4 NAFVRARIDEDLKNQAADVLAGMGLTI----SDLVRIT 37 N VRA+IDE +N AA ++ G T + L RIT Sbjct: 200 NEQVRAKIDEGTRNAAASIIEGKRATYYGIGAALARIT 237 >3WGI-A nrPDB Length = 224 Score = 25.0 bits (53), Expect = 7.7, Method: Compositional matrix adjust. Identities = 11/38 (28%), Positives = 23/38 (60%) Query: 47 LPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDLFDKL 84 LP DL+ P+ + ++++ S++ V + ++LF KL Sbjct: 178 LPIDLKVPDDVILENVHLSDSLFTVSYRLNEEELFKKL 215 >1BG3-A nrPDB Length = 918 Score = 25.0 bits (53), Expect = 8.6, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 5/64 (7%) Query: 27 GLTISDLVRITLTKVAREKALPFDLR-EPNQLTIQSIKNSEAGI---DVHKAKDADDLFD 82 G+ + +LVR+ L K+A+E L F+ R P LT S+ D ++A ++ Sbjct: 299 GMYMGELVRLILVKMAKE-GLLFEGRITPELLTRGKFNTSDVSAIEKDKEGIQNAKEILT 357 Query: 83 KLGI 86 +LG+ Sbjct: 358 RLGV 361 >1GG3-A nrPDB Length = 279 Score = 24.6 bits (52), Expect = 8.8, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 23/37 (62%), Gaps = 4/37 (10%) Query: 54 PNQLTIQSIKNSEA----GIDVHKAKDADDLFDKLGI 86 P Q ++ ++N++ G+D+HKAKD + + LG+ Sbjct: 170 PAQADLEFLENAKKLSMYGVDLHKAKDLEGVDIILGV 206 >3ZZN-A nrPDB Length = 310 Score = 24.6 bits (52), Expect = 9.3, Method: Composition-based stats. Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 7/39 (17%) Query: 8 RARIDEDLKNQAADVLAGMGLT-------ISDLVRITLT 39 RARIDE ++ A ++ G G T ++ LVR LT Sbjct: 206 RARIDEGVRRAAYRIIEGKGATYYGIGAGLARLVRAILT 244 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4Q2U-A nrPDB 157 1e-39 Sequences not found previously or not previously below threshold: 4FXE-A nrPDB 33 0.022 3FEW-X nrPDB 32 0.072 2V6B-A nrPDB 27 1.7 2MV2-A nrPDB 27 2.6 1BG3-A nrPDB 26 3.5 3WGI-A nrPDB 26 3.8 3ZZN-A nrPDB 26 4.4 4FOI-A nrPDB 25 5.3 5Z62-H nrPDB 25 6.5 CONVERGED! >4Q2U-A nrPDB Length = 86 Score = 157 bits (396), Expect = 1e-39, Method: Composition-based stats. Identities = 86/86 (100%), Positives = 86/86 (100%) Query: 1 MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQ 60 MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQ Sbjct: 1 MAANAFVRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQ 60 Query: 61 SIKNSEAGIDVHKAKDADDLFDKLGI 86 SIKNSEAGIDVHKAKDADDLFDKLGI Sbjct: 61 SIKNSEAGIDVHKAKDADDLFDKLGI 86 >4FXE-A nrPDB Length = 79 Score = 33.5 bits (75), Expect = 0.022, Method: Composition-based stats. Identities = 16/43 (37%), Positives = 27/43 (62%) Query: 7 VRARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPF 49 + RID++LK ++ L MG+T S+ +R+ L +A + LPF Sbjct: 4 INLRIDDELKARSYAALEKMGVTPSEALRLMLEYIADNERLPF 46 >3FEW-X nrPDB Length = 505 Score = 31.6 bits (70), Expect = 0.072, Method: Composition-based stats. Identities = 27/98 (27%), Positives = 46/98 (46%), Gaps = 22/98 (22%) Query: 8 RARIDEDLKNQAADVLAGMGLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIK---- 63 R RI D+ N+AA+V++ +G + D + +ARE A D++ TI+S Sbjct: 299 RDRIQSDVLNKAAEVISDIGNKVGDYLGDAYKSLAREIA--DDVKNFQGKTIRSYDDAMA 356 Query: 64 ----------------NSEAGIDVHKAKDADDLFDKLG 85 +S+A +V ++ DA D+ +KLG Sbjct: 357 SLNKVLSNPGFKFNRADSDALANVWRSIDAQDMANKLG 394 >2V6B-A nrPDB Length = 304 Score = 27.3 bits (59), Expect = 1.7, Method: Composition-based stats. Identities = 16/38 (42%), Positives = 21/38 (55%), Gaps = 4/38 (10%) Query: 4 NAFVRARIDEDLKNQAADVLAGMGLTI----SDLVRIT 37 N VRA+IDE +N AA ++ G T + L RIT Sbjct: 200 NEQVRAKIDEGTRNAAASIIEGKRATYYGIGAALARIT 237 >2MV2-A nrPDB Length = 148 Score = 26.6 bits (57), Expect = 2.6, Method: Composition-based stats. Identities = 11/47 (23%), Positives = 28/47 (59%), Gaps = 3/47 (6%) Query: 27 GLTISDLVRITLTKVAREKA---LPFDLREPNQLTIQSIKNSEAGID 70 G+T+SD+ + T ++ ++K + F +R+ Q+ ++++ + A D Sbjct: 4 GVTVSDVCKTTYEEIKKDKKHRYVIFYIRDEKQIDVETVADRNAEYD 50 >1BG3-A nrPDB Length = 918 Score = 26.2 bits (56), Expect = 3.5, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 33/64 (51%), Gaps = 5/64 (7%) Query: 27 GLTISDLVRITLTKVAREKALPFDLR-EPNQLTIQSIKNSEAGI---DVHKAKDADDLFD 82 G+ + +LVR+ L K+A+E L F+ R P LT S+ D ++A ++ Sbjct: 299 GMYMGELVRLILVKMAKE-GLLFEGRITPELLTRGKFNTSDVSAIEKDKEGIQNAKEILT 357 Query: 83 KLGI 86 +LG+ Sbjct: 358 RLGV 361 >3WGI-A nrPDB Length = 224 Score = 26.2 bits (56), Expect = 3.8, Method: Composition-based stats. Identities = 11/38 (28%), Positives = 23/38 (60%) Query: 47 LPFDLREPNQLTIQSIKNSEAGIDVHKAKDADDLFDKL 84 LP DL+ P+ + ++++ S++ V + ++LF KL Sbjct: 178 LPIDLKVPDDVILENVHLSDSLFTVSYRLNEEELFKKL 215 >3ZZN-A nrPDB Length = 310 Score = 25.8 bits (55), Expect = 4.4, Method: Composition-based stats. Identities = 15/39 (38%), Positives = 21/39 (53%), Gaps = 7/39 (17%) Query: 8 RARIDEDLKNQAADVLAGMGLT-------ISDLVRITLT 39 RARIDE ++ A ++ G G T ++ LVR LT Sbjct: 206 RARIDEGVRRAAYRIIEGKGATYYGIGAGLARLVRAILT 244 >4FOI-A nrPDB Length = 917 Score = 25.4 bits (54), Expect = 5.3, Method: Composition-based stats. Identities = 18/64 (28%), Positives = 34/64 (53%), Gaps = 5/64 (7%) Query: 27 GLTISDLVRITLTKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKD----ADDLFD 82 G+ + +LVR+ L K+A+E L F+ R +L + N+ + K K+ A ++ Sbjct: 299 GMYLGELVRLILVKMAKE-GLLFEGRITPELLTRGKFNTSDVSAIEKNKEGLHNAKEILT 357 Query: 83 KLGI 86 +LG+ Sbjct: 358 RLGV 361 >5Z62-H nrPDB Length = 82 Score = 25.4 bits (54), Expect = 6.5, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 20/39 (51%), Gaps = 2/39 (5%) Query: 39 TKVAREKALPFDLREPNQLTIQSIKNSEAGIDVHKAKDA 77 TK+ K PFD R PNQ Q+ + +D H+ + A Sbjct: 3 TKIKNYKTAPFDSRFPNQ--NQTRNCWQNYLDFHRCQKA 39 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.134 0.359 Lambda K H 0.267 0.0452 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 3,437,830 Number of Sequences: 36641 Number of extensions: 123015 Number of successful extensions: 342 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 16 Number of HSP's that attempted gapping in prelim test: 327 Number of HSP's gapped (non-prelim): 31 length of query: 86 length of database: 10,336,785 effective HSP length: 55 effective length of query: 31 effective length of database: 8,321,530 effective search space: 257967430 effective search space used: 257967430 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 53 (24.9 bits)