BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q20363 (159 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4YDZ-A nrPDB 334 8e-93 2KLR-A nrPDB 80 4e-16 5LTW-C nrPDB 60 5e-10 6F2R-D nrPDB 57 4e-09 6F2R-C nrPDB 57 4e-09 6F2R-T nrPDB 52 1e-07 4ZJA-A nrPDB 28 1.3 4WVX-A nrPDB 26 6.7 5ZNM-A nrPDB 26 8.6 >4YDZ-A nrPDB Length = 159 Score = 334 bits (857), Expect = 8e-93, Method: Compositional matrix adjust. Identities = 159/159 (100%), Positives = 159/159 (100%) Query: 1 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA 60 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA Sbjct: 1 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA 60 Query: 61 AFKPEELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM 120 AFKPEELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM Sbjct: 61 AFKPEELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM 120 Query: 121 TIDAPKTGSNTTVRALPIHTSAGHAVTQKPSSTTTTGKH 159 TIDAPKTGSNTTVRALPIHTSAGHAVTQKPSSTTTTGKH Sbjct: 121 TIDAPKTGSNTTVRALPIHTSAGHAVTQKPSSTTTTGKH 159 >2KLR-A nrPDB Length = 175 Score = 80.1 bits (196), Expect = 4e-16, Method: Compositional matrix adjust. Identities = 50/150 (33%), Positives = 73/150 (48%), Gaps = 19/150 (12%) Query: 2 SSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVAA 61 +SL P+ RP R P W F+ L+E+ +F V LDV Sbjct: 42 TSLSPFYLRPPSFLR-----APSW---------FDT----GLSEMRLEKDRFSVNLDVKH 83 Query: 62 FKPEELKVNLEGHVLTIEGHHEVK-TEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM 120 F PEELKV + G V+ + G HE + EHGF R F R++ +P DVD I + ++ +G + Sbjct: 84 FSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVL 143 Query: 121 TIDAPKTGSNTTVRALPIHTSAGHAVTQKP 150 T++ P+ + R +PI AVT P Sbjct: 144 TVNGPRKQVSGPERTIPITREEKPAVTAAP 173 >5LTW-C nrPDB Length = 149 Score = 59.7 bits (143), Expect = 5e-10, Method: Compositional matrix adjust. Identities = 38/125 (30%), Positives = 59/125 (47%), Gaps = 21/125 (16%) Query: 1 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA 60 +++LCP T + PY+ + S V + +V F V LDV Sbjct: 42 LAALCPTT------------LAPYYLRAPS--------VALPVAQVPTDPGHFSVLLDVK 81 Query: 61 AFKPEELKVNLEGHVLTIEGHHEVKT-EHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQ 119 F PEE+ V + G + + HE + EHGF R F R++ LP VD A + + ++ EG Sbjct: 82 HFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGV 141 Query: 120 MTIDA 124 ++I A Sbjct: 142 LSIQA 146 >6F2R-D nrPDB Length = 182 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 27/86 (31%), Positives = 47/86 (54%), Gaps = 1/86 (1%) Query: 44 NEVENTAQKFCVKLDVAAFKPEELKVNLEGHVLTIEGHHEVKTE-HGFSKRSFTRQFTLP 102 +E+ + KF LDV+ F P+E+ V ++L + H + + HGF R F R + LP Sbjct: 65 SELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYVLP 124 Query: 103 KDVDLAHIHTVINKEGQMTIDAPKTG 128 DVD + ++ +G + ++AP+ G Sbjct: 125 ADVDPWRVRAALSHDGILNLEAPRGG 150 >6F2R-C nrPDB Length = 203 Score = 57.0 bits (136), Expect = 4e-09, Method: Compositional matrix adjust. Identities = 27/86 (31%), Positives = 47/86 (54%), Gaps = 1/86 (1%) Query: 44 NEVENTAQKFCVKLDVAAFKPEELKVNLEGHVLTIEGHHEVKTE-HGFSKRSFTRQFTLP 102 +E+ + KF LDV+ F P+E+ V ++L + H + + HGF R F R + LP Sbjct: 86 SELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYVLP 145 Query: 103 KDVDLAHIHTVINKEGQMTIDAPKTG 128 DVD + ++ +G + ++AP+ G Sbjct: 146 ADVDPWRVRAALSHDGILNLEAPRGG 171 >6F2R-T nrPDB Length = 161 Score = 52.0 bits (123), Expect = 1e-07, Method: Compositional matrix adjust. Identities = 26/73 (35%), Positives = 40/73 (54%), Gaps = 1/73 (1%) Query: 53 FCVKLDVAAFKPEELKVN-LEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIH 111 F + LDV F PE++ + EG +L H EHGF RSFTRQ+ LP V++ + Sbjct: 71 FQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFISRSFTRQYKLPDGVEIKDLS 130 Query: 112 TVINKEGQMTIDA 124 V+ +G + ++ Sbjct: 131 AVLCHDGILVVEV 143 >4ZJA-A nrPDB Length = 147 Score = 28.5 bits (62), Expect = 1.3, Method: Compositional matrix adjust. Identities = 18/62 (29%), Positives = 27/62 (43%), Gaps = 8/62 (12%) Query: 50 AQKFCVKLDVAAFKPEELKVNLEGHVLTIEGHHEVKT--------EHGFSKRSFTRQFTL 101 A + + + V +K EEL++ G L I G H +T G K F F+L Sbjct: 51 ANNYLLTVSVPGWKEEELEIETVGGNLNITGKHTEETVEDQTHWIYRGIRKADFQLSFSL 110 Query: 102 PK 103 P+ Sbjct: 111 PE 112 >4WVX-A nrPDB Length = 326 Score = 26.2 bits (56), Expect = 6.7, Method: Compositional matrix adjust. Identities = 15/39 (38%), Positives = 18/39 (46%), Gaps = 4/39 (10%) Query: 7 YTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNE 45 + GRP L F +MM W H F NI+P NE Sbjct: 271 WLGRPFTLPEPFAEMMKNWHVEHL----FVNIIPALKNE 305 >5ZNM-A nrPDB Length = 387 Score = 25.8 bits (55), Expect = 8.6, Method: Compositional matrix adjust. Identities = 12/34 (35%), Positives = 20/34 (58%) Query: 105 VDLAHIHTVINKEGQMTIDAPKTGSNTTVRALPI 138 V+L +IN GQ+ +D KTG+ + A+P+ Sbjct: 149 VNLPVRGQLINSNGQLALDLLKTGNESIPAAVPV 182 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4YDZ-A nrPDB 248 8e-67 2KLR-A nrPDB 175 8e-45 5LTW-C nrPDB 131 1e-31 6F2R-D nrPDB 129 3e-31 6F2R-C nrPDB 129 3e-31 6F2R-T nrPDB 109 4e-25 Sequences not found previously or not previously below threshold: 3W1Z-A nrPDB 46 7e-06 1GME-A nrPDB 34 0.040 4ZJA-A nrPDB 30 0.35 5KU1-A nrPDB 30 0.57 5KSO-A nrPDB 30 0.57 2FGE-A nrPDB 28 2.0 3MSU-A nrPDB 28 2.2 3IML-A nrPDB 26 7.7 >4YDZ-A nrPDB Length = 159 Score = 248 bits (633), Expect = 8e-67, Method: Composition-based stats. Identities = 159/159 (100%), Positives = 159/159 (100%) Query: 1 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA 60 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA Sbjct: 1 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA 60 Query: 61 AFKPEELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM 120 AFKPEELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM Sbjct: 61 AFKPEELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM 120 Query: 121 TIDAPKTGSNTTVRALPIHTSAGHAVTQKPSSTTTTGKH 159 TIDAPKTGSNTTVRALPIHTSAGHAVTQKPSSTTTTGKH Sbjct: 121 TIDAPKTGSNTTVRALPIHTSAGHAVTQKPSSTTTTGKH 159 >2KLR-A nrPDB Length = 175 Score = 175 bits (443), Expect = 8e-45, Method: Composition-based stats. Identities = 50/150 (33%), Positives = 73/150 (48%), Gaps = 19/150 (12%) Query: 2 SSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVAA 61 +SL P+ RP R P W F+ L+E+ +F V LDV Sbjct: 42 TSLSPFYLRPPSFLR-----APSW---------FDT----GLSEMRLEKDRFSVNLDVKH 83 Query: 62 FKPEELKVNLEGHVLTIEGHHEVK-TEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQM 120 F PEELKV + G V+ + G HE + EHGF R F R++ +P DVD I + ++ +G + Sbjct: 84 FSPEELKVKVLGDVIEVHGKHEERQDEHGFISREFHRKYRIPADVDPLTITSSLSSDGVL 143 Query: 121 TIDAPKTGSNTTVRALPIHTSAGHAVTQKP 150 T++ P+ + R +PI AVT P Sbjct: 144 TVNGPRKQVSGPERTIPITREEKPAVTAAP 173 >5LTW-C nrPDB Length = 149 Score = 131 bits (330), Expect = 1e-31, Method: Composition-based stats. Identities = 38/125 (30%), Positives = 59/125 (47%), Gaps = 21/125 (16%) Query: 1 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVA 60 +++LCP T + PY+ + S V + +V F V LDV Sbjct: 42 LAALCPTT------------LAPYYLRAPS--------VALPVAQVPTDPGHFSVLLDVK 81 Query: 61 AFKPEELKVNLEGHVLTIEGHHEVK-TEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQ 119 F PEE+ V + G + + HE + EHGF R F R++ LP VD A + + ++ EG Sbjct: 82 HFSPEEIAVKVVGEHVEVHARHEERPDEHGFVAREFHRRYRLPPGVDPAAVTSALSPEGV 141 Query: 120 MTIDA 124 ++I A Sbjct: 142 LSIQA 146 >6F2R-D nrPDB Length = 182 Score = 129 bits (325), Expect = 3e-31, Method: Composition-based stats. Identities = 27/88 (30%), Positives = 47/88 (53%), Gaps = 1/88 (1%) Query: 42 QLNEVENTAQKFCVKLDVAAFKPEELKVNLEGHVLTIEGHHEVKTE-HGFSKRSFTRQFT 100 +E+ + KF LDV+ F P+E+ V ++L + H + + HGF R F R + Sbjct: 63 GASELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYV 122 Query: 101 LPKDVDLAHIHTVINKEGQMTIDAPKTG 128 LP DVD + ++ +G + ++AP+ G Sbjct: 123 LPADVDPWRVRAALSHDGILNLEAPRGG 150 >6F2R-C nrPDB Length = 203 Score = 129 bits (325), Expect = 3e-31, Method: Composition-based stats. Identities = 27/88 (30%), Positives = 47/88 (53%), Gaps = 1/88 (1%) Query: 42 QLNEVENTAQKFCVKLDVAAFKPEELKVNLEGHVLTIEGHHEVKTE-HGFSKRSFTRQFT 100 +E+ + KF LDV+ F P+E+ V ++L + H + + HGF R F R + Sbjct: 84 GASELRLSEGKFQAFLDVSHFTPDEVTVRTVDNLLEVSARHPQRLDRHGFVSREFCRTYV 143 Query: 101 LPKDVDLAHIHTVINKEGQMTIDAPKTG 128 LP DVD + ++ +G + ++AP+ G Sbjct: 144 LPADVDPWRVRAALSHDGILNLEAPRGG 171 >6F2R-T nrPDB Length = 161 Score = 109 bits (273), Expect = 4e-25, Method: Composition-based stats. Identities = 26/79 (32%), Positives = 40/79 (50%), Gaps = 1/79 (1%) Query: 47 ENTAQKFCVKLDVAAFKPEELKVN-LEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDV 105 F + LDV F PE++ + EG +L H EHGF RSFTRQ+ LP V Sbjct: 65 REGKSHFQILLDVVQFLPEDIIIQTFEGWLLIKAQHGTRMDEHGFISRSFTRQYKLPDGV 124 Query: 106 DLAHIHTVINKEGQMTIDA 124 ++ + V+ +G + ++ Sbjct: 125 EIKDLSAVLCHDGILVVEV 143 >3W1Z-A nrPDB Length = 143 Score = 46.0 bits (107), Expect = 7e-06, Method: Composition-based stats. Identities = 41/149 (27%), Positives = 68/149 (45%), Gaps = 21/149 (14%) Query: 3 SLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLN-EVENTAQKFCVKLDVA- 60 SL P+ G P + F D + Y + NN +P +L+ ++ K V +DV Sbjct: 2 SLQPFFGFPPTVNDLFSDFVSYSPR-------LNNQIPGELSPSIDVHEGKDTVSVDVEL 54 Query: 61 -AFKPEELKVNLEGHVLTIEGH--HEVKTE-----HGFSKR---SFTRQFTLPKDVDLAH 109 K E+++V+ + LTI G +E K E +S+R SF+R T+P +D Sbjct: 55 PGVKKEDVQVHYDSGKLTISGEVVNERKNESTEGNQRWSERRFGSFSRTITIPAKIDADR 114 Query: 110 IHTVINKEGQMTIDAPKTGSNTTVRALPI 138 I + G +T+ PK + T + + I Sbjct: 115 IEANFS-NGLLTVTLPKVEKSQTKKQIAI 142 >1GME-A nrPDB Length = 151 Score = 33.7 bits (75), Expect = 0.040, Method: Composition-based stats. Identities = 18/76 (23%), Positives = 31/76 (40%), Gaps = 10/76 (13%) Query: 73 GHVLTIEGHHEVKTE------HGF--SKRSFTRQFTLPKDVDLAHIHTVINKEGQMTIDA 124 G+VL + G + E H S F R+F L +D + + + + G +T+ Sbjct: 76 GNVLVVSGERTKEKEDKNDKWHRVERSSGKFVRRFRLLEDAKVEEVKAGL-ENGVLTVTV 134 Query: 125 PKTGSNTTV-RALPIH 139 PK +A+ I Sbjct: 135 PKAEVKKPEVKAIQIS 150 >4ZJA-A nrPDB Length = 147 Score = 30.2 bits (66), Expect = 0.35, Method: Composition-based stats. Identities = 28/132 (21%), Positives = 51/132 (38%), Gaps = 12/132 (9%) Query: 1 MSSLCPYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEV-ENTAQKFCVKLDV 59 M +L + P F D + S L + ++ + A + + + V Sbjct: 1 MMALRTLSALPVFADSLFSDRFNRIDRLFSQLTGDTPVAATPAYDLQKRDANNYLLTVSV 60 Query: 60 AAFKPEELKVNLEGHVLTIEGHHEVKT----EH----GFSKRSFTRQFTLPKDVDLAHIH 111 +K EEL++ G L I G H +T H G K F F+LP + A ++ Sbjct: 61 PGWKEEELEIETVGGNLNITGKHTEETVEDQTHWIYRGIRKADFQLSFSLP---EHAKVN 117 Query: 112 TVINKEGQMTID 123 ++G + ++ Sbjct: 118 NAKLEQGLLLVE 129 >5KU1-A nrPDB Length = 424 Score = 29.8 bits (65), Expect = 0.57, Method: Composition-based stats. Identities = 26/124 (20%), Positives = 39/124 (31%), Gaps = 25/124 (20%) Query: 43 LNEVENTAQKFCVKLDVAAFKPEELKVNLEGHV--------LTIEGH---HEVKTEHGFS 91 L E E C+ DV+ K E + L + HEVK E+ S Sbjct: 306 LTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSIS 365 Query: 92 KRSFTRQFTLPKDVDLAHIHTVINKEGQMTIDAPKTGSNTTVRALPIHTSAGHAVTQKPS 151 F R+ +P T DAP + + ++ VTQ Sbjct: 366 PTDFCRKHKMPP---PQAFTCN-------TADAPSKDIFVKLTTMAMY----PHVTQADL 411 Query: 152 STTT 155 ++T Sbjct: 412 KSST 415 >5KSO-A nrPDB Length = 190 Score = 29.8 bits (65), Expect = 0.57, Method: Composition-based stats. Identities = 26/124 (20%), Positives = 39/124 (31%), Gaps = 25/124 (20%) Query: 43 LNEVENTAQKFCVKLDVAAFKPEELKVNLEGHV--------LTIEGH---HEVKTEHGFS 91 L E E C+ DV+ K E + L + HEVK E+ S Sbjct: 72 LTEAEIICDVVCLVYDVSNPKSFEYCARIFKQHFMDSRIPCLIVAAKSDLHEVKQEYSIS 131 Query: 92 KRSFTRQFTLPKDVDLAHIHTVINKEGQMTIDAPKTGSNTTVRALPIHTSAGHAVTQKPS 151 F R+ +P T DAP + + ++ VTQ Sbjct: 132 PTDFCRKHKMPP---PQAFTCN-------TADAPSKDIFVKLTTMAMY----PHVTQADL 177 Query: 152 STTT 155 ++T Sbjct: 178 KSST 181 >2FGE-A nrPDB Length = 995 Score = 27.9 bits (60), Expect = 2.0, Method: Composition-based stats. Identities = 18/119 (15%), Positives = 35/119 (29%), Gaps = 6/119 (5%) Query: 6 PYTGRPTGLFRDFEDMMPYWAQRHSMLNNFNNIVPQQLNEVENTAQKFCVKLDVAAFKPE 65 P+ G F + Y + + + N L +V A F +D A Sbjct: 95 PFVELLKGSLHTFLNAFTYPDRTCYPVASTNTKDFYNLVDVYLDAVFFPKCVDDAH---- 150 Query: 66 ELKVNLEGHVLTIEGHHEVKTEHGFSKRSFTRQFTLPKDVDLAHIHTVINKEGQMTIDA 124 EG + E + G ++ P ++ ++ E +D+ Sbjct: 151 --TFQQEGWHYELNDPSEDISYKGVVFNEMKGVYSQPDNILGRIAQQALSPENTYGVDS 207 >3MSU-A nrPDB Length = 427 Score = 27.9 bits (60), Expect = 2.2, Method: Composition-based stats. Identities = 7/32 (21%), Positives = 15/32 (46%) Query: 53 FCVKLDVAAFKPEELKVNLEGHVLTIEGHHEV 84 + + D ++KP+EL + + + HE Sbjct: 205 YMMFADDESYKPDELHIKAMDTIFMLHADHEQ 236 >3IML-A nrPDB Length = 399 Score = 26.0 bits (55), Expect = 7.7, Method: Composition-based stats. Identities = 12/41 (29%), Positives = 19/41 (46%) Query: 100 TLPKDVDLAHIHTVINKEGQMTIDAPKTGSNTTVRALPIHT 140 TLP D+ I ++N G+ I P+ T R + + T Sbjct: 218 TLPADLIKGDIKFLVNPTGRFVIGGPQGDCGLTGRKIIVDT 258 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.303 0.117 0.306 Lambda K H 0.267 0.0347 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 5,396,886 Number of Sequences: 36641 Number of extensions: 182268 Number of successful extensions: 503 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 20 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 471 Number of HSP's gapped (non-prelim): 28 length of query: 159 length of database: 10,336,785 effective HSP length: 89 effective length of query: 70 effective length of database: 7,075,736 effective search space: 495301520 effective search space used: 495301520 T: 11 A: 40 X1: 16 ( 7.0 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.0 bits) S2: 54 (25.6 bits)