BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q18235 (244 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5MZ6-B nrPDB 498 e-141 2AWD-A nrPDB 31 0.50 2WKX-A nrPDB 31 0.60 3N6R-B nrPDB 29 2.1 2D4G-A nrPDB 28 5.3 3L2D-A nrPDB 27 7.6 3VPX-A nrPDB 27 8.3 5X49-A nrPDB 27 9.3 >5MZ6-B nrPDB Length = 244 Score = 498 bits (1281), Expect = e-141, Method: Compositional matrix adjust. Identities = 244/244 (100%), Positives = 244/244 (100%) Query: 1 MEDLNFEERGSTQIPASLQQHFSAKLGRQNELEKTPSRGGLGLVVNSSKTPGGKSLQSLA 60 MEDLNFEERGSTQIPASLQQHFSAKLGRQNELEKTPSRGGLGLVVNSSKTPGGKSLQSLA Sbjct: 1 MEDLNFEERGSTQIPASLQQHFSAKLGRQNELEKTPSRGGLGLVVNSSKTPGGKSLQSLA 60 Query: 61 SACKVPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPCSPIDTANRCETFDSLAA 120 SACKVPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPCSPIDTANRCETFDSLAA Sbjct: 61 SACKVPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPCSPIDTANRCETFDSLAA 120 Query: 121 DIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAEALAELGVEEWDSYPPIDPASRIGDDF 180 DIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAEALAELGVEEWDSYPPIDPASRIGDDF Sbjct: 121 DIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAEALAELGVEEWDSYPPIDPASRIGDDF 180 Query: 181 NYVLRTEDFAEEGDVKLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEEA 240 NYVLRTEDFAEEGDVKLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEEA Sbjct: 181 NYVLRTEDFAEEGDVKLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEEA 240 Query: 241 NLPL 244 NLPL Sbjct: 241 NLPL 244 >2AWD-A nrPDB Length = 323 Score = 30.8 bits (68), Expect = 0.50, Method: Compositional matrix adjust. Identities = 28/93 (30%), Positives = 38/93 (40%), Gaps = 22/93 (23%) Query: 48 SKTPGGKSLQS-----------LASACK-------VPPSTKKNTIPIAFECYEDETDDQI 89 +KTPGGK L +A+ + KK IP AF ++ET D I Sbjct: 34 TKTPGGKGLNVTRVIHDLGGDVIATGVLGGFHGAFIANELKKANIPQAFTSIKEETRDSI 93 Query: 90 ADVATIKKTE----KHPCSPIDTANRCETFDSL 118 A + +TE SP + +N E FD L Sbjct: 94 AILHEGNQTEILEAGPTVSPEEISNFLENFDQL 126 >2WKX-A nrPDB Length = 261 Score = 30.8 bits (68), Expect = 0.60, Method: Compositional matrix adjust. Identities = 18/67 (26%), Positives = 36/67 (53%), Gaps = 2/67 (2%) Query: 100 KHPCSPIDTANRCETFDSLAADIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAE--ALA 157 + P +P+DTA+ E D++ DM E + V+++ + + E++AE A+A Sbjct: 192 RAPHTPVDTASLLELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIA 251 Query: 158 ELGVEEW 164 E +E++ Sbjct: 252 EALLEKY 258 >3N6R-B nrPDB Length = 531 Score = 28.9 bits (63), Expect = 2.1, Method: Compositional matrix adjust. Identities = 30/131 (22%), Positives = 47/131 (35%), Gaps = 9/131 (6%) Query: 103 CSPIDTANRCETFDSLAADIEDDMLNLEDQDVVL-SEDRPYGDVIDPAESEAEALAELGV 161 C ID++ + F E +L L D L + YG VI A E V Sbjct: 353 CLDIDSSRKAARFVRFCDAFEIPLLTLIDVPGFLPGTSQEYGGVIKHGAKLLYAYGEATV 412 Query: 162 --------EEWDSYPPIDPASRIGDDFNYVLRTEDFAEEGDVKLEETRHRTVIADIDEVK 213 + + + + + DFNY T + A G E HR + D +++ Sbjct: 413 PMVTVITRKAYGGAYVVMSSKHLRADFNYAWPTAEVAVMGAKGATEIIHRGDLGDPEKIA 472 Query: 214 MSKAERNELFS 224 A+ E F+ Sbjct: 473 QHTADYEERFA 483 >2D4G-A nrPDB Length = 171 Score = 27.7 bits (60), Expect = 5.3, Method: Compositional matrix adjust. Identities = 18/59 (30%), Positives = 29/59 (49%), Gaps = 13/59 (22%) Query: 54 KSLQSLASACK---------VPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPC 103 K LQ LA++ + +PP T+ +FEC E++ D ++ + I K E HP Sbjct: 11 KKLQDLANSYRKRYDPSYSLIPPHL---TLRASFECAEEKADQLVSHLRNIAK-ESHPL 65 >3L2D-A nrPDB Length = 390 Score = 26.9 bits (58), Expect = 7.6, Method: Compositional matrix adjust. Identities = 10/31 (32%), Positives = 19/31 (61%) Query: 77 AFECYEDETDDQIADVATIKKTEKHPCSPID 107 ++ECY++ D I ++ K ++KHP +D Sbjct: 93 SYECYKEFFDKCIEEIHHFKPSDKHPAPDLD 123 >3VPX-A nrPDB Length = 364 Score = 26.9 bits (58), Expect = 8.3, Method: Compositional matrix adjust. Identities = 23/90 (25%), Positives = 43/90 (47%), Gaps = 1/90 (1%) Query: 146 IDPAESEAEALAELGVEEWDSYPPIDPASRIGDDFNYVLRTEDFAEEGDVKLEETRHRTV 205 +D + A + E+ +E D + P + I D+ L+ + A + +L+ETRH + Sbjct: 213 VDAFGATAVGINEIYSQEADIFAPCALGAIINDETIPQLKAKVIAGSANNQLKETRHGDL 272 Query: 206 IADIDEVKMSKAERNELFSM-LADDLDSYD 234 I ++ V N + +AD+LD Y+ Sbjct: 273 IHEMGIVYAPDYVINSGGVINVADELDGYN 302 >5X49-A nrPDB Length = 456 Score = 26.6 bits (57), Expect = 9.3, Method: Compositional matrix adjust. Identities = 12/29 (41%), Positives = 17/29 (58%) Query: 113 ETFDSLAADIEDDMLNLEDQDVVLSEDRP 141 E F L IEDD++ +D ++LS D P Sbjct: 414 EKFRGLGVRIEDDVVVTQDSPLILSADCP 442 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5MZ6-B nrPDB 490 e-139 Sequences not found previously or not previously below threshold: 2AWD-A nrPDB 33 0.16 2WKX-A nrPDB 32 0.18 5X49-A nrPDB 30 0.76 2BZT-A nrPDB 29 1.5 3L2D-A nrPDB 29 1.5 1UJ8-A nrPDB 29 1.6 5JRE-A nrPDB 29 1.9 CONVERGED! >5MZ6-B nrPDB Length = 244 Score = 490 bits (1261), Expect = e-139, Method: Composition-based stats. Identities = 244/244 (100%), Positives = 244/244 (100%) Query: 1 MEDLNFEERGSTQIPASLQQHFSAKLGRQNELEKTPSRGGLGLVVNSSKTPGGKSLQSLA 60 MEDLNFEERGSTQIPASLQQHFSAKLGRQNELEKTPSRGGLGLVVNSSKTPGGKSLQSLA Sbjct: 1 MEDLNFEERGSTQIPASLQQHFSAKLGRQNELEKTPSRGGLGLVVNSSKTPGGKSLQSLA 60 Query: 61 SACKVPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPCSPIDTANRCETFDSLAA 120 SACKVPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPCSPIDTANRCETFDSLAA Sbjct: 61 SACKVPPSTKKNTIPIAFECYEDETDDQIADVATIKKTEKHPCSPIDTANRCETFDSLAA 120 Query: 121 DIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAEALAELGVEEWDSYPPIDPASRIGDDF 180 DIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAEALAELGVEEWDSYPPIDPASRIGDDF Sbjct: 121 DIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAEALAELGVEEWDSYPPIDPASRIGDDF 180 Query: 181 NYVLRTEDFAEEGDVKLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEEA 240 NYVLRTEDFAEEGDVKLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEEA Sbjct: 181 NYVLRTEDFAEEGDVKLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEEA 240 Query: 241 NLPL 244 NLPL Sbjct: 241 NLPL 244 >2AWD-A nrPDB Length = 323 Score = 32.8 bits (73), Expect = 0.16, Method: Composition-based stats. Identities = 32/107 (29%), Positives = 45/107 (42%), Gaps = 11/107 (10%) Query: 26 LGRQNELEKTPSRGGLGL-VVNSSKTPGGKSLQSLA----SACKVPPSTKKNTIPIAFEC 80 + R +++ KTP GG GL V GG + + + KK IP AF Sbjct: 27 VNRTSQVTKTP--GGKGLNVTRVIHDLGGDVIATGVLGGFHGAFIANELKKANIPQAFTS 84 Query: 81 YEDETDDQIADVATIKKTE----KHPCSPIDTANRCETFDSLAADIE 123 ++ET D IA + +TE SP + +N E FD L E Sbjct: 85 IKEETRDSIAILHEGNQTEILEAGPTVSPEEISNFLENFDQLIKQAE 131 >2WKX-A nrPDB Length = 261 Score = 32.4 bits (72), Expect = 0.18, Method: Composition-based stats. Identities = 18/67 (26%), Positives = 36/67 (53%), Gaps = 2/67 (2%) Query: 100 KHPCSPIDTANRCETFDSLAADIEDDMLNLEDQDVVLSEDRPYGDVIDPAESEAE--ALA 157 + P +P+DTA+ E D++ DM E + V+++ + + E++AE A+A Sbjct: 192 RAPHTPVDTASLLELLARYGYDVKPDMTPREQRRVIMAFQMHFRPTLYNGEADAETQAIA 251 Query: 158 ELGVEEW 164 E +E++ Sbjct: 252 EALLEKY 258 >5X49-A nrPDB Length = 456 Score = 30.5 bits (67), Expect = 0.76, Method: Composition-based stats. Identities = 14/37 (37%), Positives = 20/37 (54%) Query: 105 PIDTANRCETFDSLAADIEDDMLNLEDQDVVLSEDRP 141 P D + E F L IEDD++ +D ++LS D P Sbjct: 406 PEDDKDAPEKFRGLGVRIEDDVVVTQDSPLILSADCP 442 >2BZT-A nrPDB Length = 66 Score = 29.3 bits (64), Expect = 1.5, Method: Composition-based stats. Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Query: 158 ELGVEEWDSYPPIDPAS-RIGDDFNYVLRTEDFAEEGDVKLEETRHRTVIADIDEVK 213 E+G +D+YP +DP + R D ++ EDF ++ E+ ++ +DE + Sbjct: 10 EIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDDDPQASNEKILEAILLVWLDEAE 66 >3L2D-A nrPDB Length = 390 Score = 29.3 bits (64), Expect = 1.5, Method: Composition-based stats. Identities = 10/31 (32%), Positives = 19/31 (61%) Query: 77 AFECYEDETDDQIADVATIKKTEKHPCSPID 107 ++ECY++ D I ++ K ++KHP +D Sbjct: 93 SYECYKEFFDKCIEEIHHFKPSDKHPAPDLD 123 >1UJ8-A nrPDB Length = 77 Score = 29.3 bits (64), Expect = 1.6, Method: Composition-based stats. Identities = 15/57 (26%), Positives = 29/57 (50%), Gaps = 1/57 (1%) Query: 158 ELGVEEWDSYPPIDPAS-RIGDDFNYVLRTEDFAEEGDVKLEETRHRTVIADIDEVK 213 E+G +D+YP +DP + R D ++ EDF ++ E+ ++ +DE + Sbjct: 21 EIGEALYDAYPDLDPKTVRFTDMHQWICDLEDFDDDPQASNEKILEAILLVWLDEAE 77 >5JRE-A nrPDB Length = 219 Score = 28.9 bits (63), Expect = 1.9, Method: Composition-based stats. Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 3/44 (6%) Query: 196 KLEETRHRTVIADIDEVKMSKAERNELFSMLADDLDSYDLLAEE 239 KLEE + V I +MSK ++ ++SM+ +D S D EE Sbjct: 48 KLEEKKQEIVDRSI---RMSKLSKSLIYSMIREDYKSADKYKEE 88 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.310 0.130 0.365 Lambda K H 0.267 0.0401 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 9,683,773 Number of Sequences: 36641 Number of extensions: 390238 Number of successful extensions: 772 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 8 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 764 Number of HSP's gapped (non-prelim): 23 length of query: 244 length of database: 10,336,785 effective HSP length: 94 effective length of query: 150 effective length of database: 6,892,531 effective search space: 1033879650 effective search space used: 1033879650 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 58 (27.0 bits)