BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q16658 (493 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 3P53-A nrPDB 1028 0.0 >3P53-A nrPDB Length = 496 Score = 1028 bits (2657), Expect = 0.0, Method: Compositional matrix adjust. Identities = 493/493 (100%), Positives = 493/493 (100%) Query: 1 MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAV 60 MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAV Sbjct: 4 MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAV 63 Query: 61 CLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLS 120 CLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLS Sbjct: 64 CLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLS 123 Query: 121 CFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLA 180 CFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLA Sbjct: 124 CFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLA 183 Query: 181 FQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTL 240 FQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTL Sbjct: 184 FQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTL 243 Query: 241 KAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR 300 KAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR Sbjct: 244 KAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR 303 Query: 301 DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKN 360 DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKN Sbjct: 304 DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKN 363 Query: 361 GQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND 420 GQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND Sbjct: 364 GQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND 423 Query: 421 GAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA 480 GAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA Sbjct: 424 GAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA 483 Query: 481 SAETVDPASLWEY 493 SAETVDPASLWEY Sbjct: 484 SAETVDPASLWEY 496 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 3P53-A nrPDB 1064 0.0 Sequences not found previously or not previously below threshold: 4XA6-A nrPDB 30 2.7 1YNA-A nrPDB 28 7.9 1Z4S-A nrPDB 28 9.0 CONVERGED! >3P53-A nrPDB Length = 496 Score = 1064 bits (2751), Expect = 0.0, Method: Composition-based stats. Identities = 493/493 (100%), Positives = 493/493 (100%) Query: 1 MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAV 60 MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAV Sbjct: 4 MTANGTAEAVQIQFGLINCGNKYLTAEAFGFKVNASASSLKKKQIWTLEQPPDEAGSAAV 63 Query: 61 CLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLS 120 CLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLS Sbjct: 64 CLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAHRRYFGGTEDRLS 123 Query: 121 CFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLA 180 CFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLA Sbjct: 124 CFAQTVSPAEKWSVHIAMHPQVNIYSVTRKRYAHLSARPADEIAVDRDVPWGVDSLITLA 183 Query: 181 FQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTL 240 FQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTL Sbjct: 184 FQDQRYSVQTADHRFLRHDGRLVARPEPATGYTLEFRSGKVAFRDCEGRYLAPSGPSGTL 243 Query: 241 KAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR 300 KAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR Sbjct: 244 KAGKATKVGKDELFALEQSCAQVVLQAANERNVSTRQGMDLSANQDEETDQETFQLEIDR 303 Query: 301 DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKN 360 DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKN Sbjct: 304 DTKKCAFRTHTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKN 363 Query: 361 GQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND 420 GQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND Sbjct: 364 GQLAASVETAGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFND 423 Query: 421 GAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA 480 GAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA Sbjct: 424 GAYNIKDSTGKYWTVGSDSAVTSSGDTPVDFFFEFCDYNKVAIKVGGRYLKGDHAGVLKA 483 Query: 481 SAETVDPASLWEY 493 SAETVDPASLWEY Sbjct: 484 SAETVDPASLWEY 496 >4XA6-A nrPDB Length = 175 Score = 30.0 bits (66), Expect = 2.7, Method: Composition-based stats. Identities = 20/59 (33%), Positives = 31/59 (52%), Gaps = 2/59 (3%) Query: 245 ATKVGKDELFALEQSCAQVV--LQAANERNVSTRQGMDLSANQDEETDQETFQLEIDRD 301 A K GK +L LE ++ L+A +RN + +GM S + +E +T LE +RD Sbjct: 81 ALKGGKKQLQKLEARVRELENELEAEQKRNAESVKGMRKSERRIKELTYQTEDLEKERD 139 >1YNA-A nrPDB Length = 194 Score = 28.1 bits (61), Expect = 7.9, Method: Composition-based stats. Identities = 29/155 (18%), Positives = 56/155 (36%), Gaps = 21/155 (13%) Query: 310 HTGKYWTLTATGGVQSTASSKNASCYFDIEWRDRRITLRASNGKFVTSKKNGQLAASVET 369 H G Y++ + GG Q+T ++ Y +I W D + + + + Sbjct: 10 HDGYYYSWWSDGGAQATYTNLEGGTY-EISWGDGGNLVGGKGWNPGLNARAIHFEGVYQP 68 Query: 370 AGDSELFLMKLINRPIIVFRGEHGFIGCRKVTGTLDANRSSYDVFQLEFNDGAYNIKDST 429 G+S L + P++ + F GT D + + D+ +E + Y + +T Sbjct: 69 NGNSYLAVYGWTRNPLVEYYIVENF-------GTYDPSSGATDLGTVECDGSIYRLGKTT 121 Query: 430 -------------GKYWTVGSDSAVTSSGDTPVDF 451 +YW+V D + + T F Sbjct: 122 RVNAPSIDGTQTFDQYWSVRQDKRTSGTVQTGCHF 156 >1Z4S-A nrPDB Length = 144 Score = 28.1 bits (61), Expect = 9.0, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 32/64 (50%), Gaps = 3/64 (4%) Query: 60 VCLRSHLGRYLAADKDGNVTCEREVPGPDCRFLIVAHDDGRWSLQSEAH--RRYFGGTED 117 V ++S G+YLA D DG + + P +C FL ++ + S+ H + +F G + Sbjct: 59 VYIKSTTGQYLAMDTDG-LLYGSQTPNEECLFLERLEENHYNTYISKKHAEKNWFVGLKK 117 Query: 118 RLSC 121 SC Sbjct: 118 NGSC 121 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.132 0.399 Lambda K H 0.267 0.0407 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 20,495,358 Number of Sequences: 36641 Number of extensions: 868124 Number of successful extensions: 1948 Number of sequences better than 10.0: 5 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 1922 Number of HSP's gapped (non-prelim): 14 length of query: 493 length of database: 10,336,785 effective HSP length: 101 effective length of query: 392 effective length of database: 6,636,044 effective search space: 2601329248 effective search space used: 2601329248 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 61 (28.1 bits)