BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q16552 (155 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5N7W-X nrPDB 319 4e-88 5HI3-A nrPDB 275 5e-75 5N92-F nrPDB 124 2e-29 1TII-A nrPDB 25 9.6 >5N7W-X nrPDB Length = 155 Score = 319 bits (817), Expect = 4e-88, Method: Compositional matrix adjust. Identities = 155/155 (100%), Positives = 155/155 (100%) Query: 1 MTPGKTSLVSLLLLLSLEAIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNP 60 MTPGKTSLVSLLLLLSLEAIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNP Sbjct: 1 MTPGKTSLVSLLLLLSLEAIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNP 60 Query: 61 KRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEIL 120 KRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEIL Sbjct: 61 KRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEIL 120 Query: 121 VLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA 155 VLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA Sbjct: 121 VLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA 155 >5HI3-A nrPDB Length = 132 Score = 275 bits (704), Expect = 5e-75, Method: Compositional matrix adjust. Identities = 132/132 (100%), Positives = 132/132 (100%) Query: 24 GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPE 83 GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPE Sbjct: 1 GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPE 60 Query: 84 RYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVG 143 RYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVG Sbjct: 61 RYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVG 120 Query: 144 CTCVTPIVHHVA 155 CTCVTPIVHHVA Sbjct: 121 CTCVTPIVHHVA 132 >5N92-F nrPDB Length = 139 Score = 124 bits (310), Expect = 2e-29, Method: Compositional matrix adjust. Identities = 61/108 (56%), Positives = 76/108 (70%) Query: 47 VNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNV 106 + L+I N N S + +RSTSPWN DP RYPS + +A+CR+LGCINA G Sbjct: 31 MKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 90 Query: 107 DYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHV 154 D MNSVPIQQE LV+RR+ C SF+LEK+LV+VGCTCVTP++HHV Sbjct: 91 DISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHV 138 >1TII-A nrPDB Length = 190 Score = 25.4 bits (54), Expect = 9.6, Method: Compositional matrix adjust. Identities = 16/57 (28%), Positives = 29/57 (50%), Gaps = 8/57 (14%) Query: 18 EAIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNI--HNRNTNTNPKRSSDYYNRSTS 72 + + ++G IPR +D+ + R +N+N+ H R T T R +D Y +T+ Sbjct: 12 DEVRRSGGLIPR------GQDEAYERGTPININLYDHARGTATGNTRYNDGYVSTTT 62 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5N7W-X nrPDB 283 2e-77 5HI3-A nrPDB 242 4e-65 5N92-F nrPDB 193 3e-50 Sequences not found previously or not previously below threshold: 5WAC-A nrPDB 28 1.9 CONVERGED! >5N7W-X nrPDB Length = 155 Score = 283 bits (725), Expect = 2e-77, Method: Composition-based stats. Identities = 155/155 (100%), Positives = 155/155 (100%) Query: 1 MTPGKTSLVSLLLLLSLEAIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNP 60 MTPGKTSLVSLLLLLSLEAIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNP Sbjct: 1 MTPGKTSLVSLLLLLSLEAIVKAGITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNP 60 Query: 61 KRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEIL 120 KRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEIL Sbjct: 61 KRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEIL 120 Query: 121 VLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA 155 VLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA Sbjct: 121 VLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHVA 155 >5HI3-A nrPDB Length = 132 Score = 242 bits (618), Expect = 4e-65, Method: Composition-based stats. Identities = 132/132 (100%), Positives = 132/132 (100%) Query: 24 GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPE 83 GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPE Sbjct: 1 GITIPRNPGCPNSEDKNFPRTVMVNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPE 60 Query: 84 RYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVG 143 RYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVG Sbjct: 61 RYPSVIWEAKCRHLGCINADGNVDYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVG 120 Query: 144 CTCVTPIVHHVA 155 CTCVTPIVHHVA Sbjct: 121 CTCVTPIVHHVA 132 >5N92-F nrPDB Length = 139 Score = 193 bits (490), Expect = 3e-50, Method: Composition-based stats. Identities = 61/108 (56%), Positives = 76/108 (70%) Query: 47 VNLNIHNRNTNTNPKRSSDYYNRSTSPWNLHRNEDPERYPSVIWEAKCRHLGCINADGNV 106 + L+I N N S + +RSTSPWN DP RYPS + +A+CR+LGCINA G Sbjct: 31 MKLDIGIINENQRVSMSRNIESRSTSPWNYTVTWDPNRYPSEVVQAQCRNLGCINAQGKE 90 Query: 107 DYHMNSVPIQQEILVLRREPPHCPNSFRLEKILVSVGCTCVTPIVHHV 154 D MNSVPIQQE LV+RR+ C SF+LEK+LV+VGCTCVTP++HHV Sbjct: 91 DISMNSVPIQQETLVVRRKHQGCSVSFQLEKVLVTVGCTCVTPVIHHV 138 >5WAC-A nrPDB Length = 361 Score = 27.7 bits (60), Expect = 1.9, Method: Composition-based stats. Identities = 18/62 (29%), Positives = 26/62 (41%), Gaps = 9/62 (14%) Query: 40 NFPRTVMVNLNIHNRNTNTNPKRSSD------YYNRSTSPWNL---HRNEDPERYPSVIW 90 N P+T M N N + + Y +ST P L H N +P++YP+ I Sbjct: 192 NVPKTQMQNYAFGYNQENQPIRVNPGPLDAPAYGVKSTLPDMLSFIHANLNPQKYPTDIQ 251 Query: 91 EA 92 A Sbjct: 252 RA 253 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.136 0.413 Lambda K H 0.267 0.0413 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 6,762,027 Number of Sequences: 36641 Number of extensions: 252228 Number of successful extensions: 472 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 3 Number of HSP's that attempted gapping in prelim test: 465 Number of HSP's gapped (non-prelim): 10 length of query: 155 length of database: 10,336,785 effective HSP length: 89 effective length of query: 66 effective length of database: 7,075,736 effective search space: 466998576 effective search space used: 466998576 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 55 (25.8 bits)