BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q15544 (211 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6MZL-P nrPDB 427 e-120 1BH8-B nrPDB 189 9e-49 1BMV-2 nrPDB 32 0.22 >6MZL-P nrPDB Length = 211 Score = 427 bits (1099), Expect = e-120, Method: Compositional matrix adjust. Identities = 211/211 (100%), Positives = 211/211 (100%) Query: 1 MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELES 60 MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELES Sbjct: 1 MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELES 60 Query: 61 QDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNR 120 QDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNR Sbjct: 61 QDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNR 120 Query: 121 YEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMP 180 YEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMP Sbjct: 121 YEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMP 180 Query: 181 PLQPKHMREAVRRLKSKGQIPNSKHKKIIFF 211 PLQPKHMREAVRRLKSKGQIPNSKHKKIIFF Sbjct: 181 PLQPKHMREAVRRLKSKGQIPNSKHKKIIFF 211 >1BH8-B nrPDB Length = 89 Score = 189 bits (479), Expect = 9e-49, Method: Compositional matrix adjust. Identities = 89/89 (100%), Positives = 89/89 (100%) Query: 113 FSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDV 172 FSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDV Sbjct: 1 FSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDV 60 Query: 173 CEKWGEMPPLQPKHMREAVRRLKSKGQIP 201 CEKWGEMPPLQPKHMREAVRRLKSKGQIP Sbjct: 61 CEKWGEMPPLQPKHMREAVRRLKSKGQIP 89 >1BMV-2 nrPDB Length = 374 Score = 32.0 bits (71), Expect = 0.22, Method: Compositional matrix adjust. Identities = 41/182 (22%), Positives = 67/182 (36%), Gaps = 53/182 (29%) Query: 33 TDGIPEETDGDADVDLKEAAAEEGELESQDVSDLTTVEREDSSLLNPAAKKLKIDTKEKK 92 T GIPE T + A A + + SD+ T+ +D L NPA K + Sbjct: 77 TVGIPENTG------IALACAMNSSIRGRASSDIYTICSQDCELWNPACTKAMTMSFNPN 130 Query: 93 E----------KKQKVDEDEI----------QKMQILVSSFSEEQ--------------- 117 K+ D I Q +Q+ + F Q Sbjct: 131 PCSDAWSLEFLKRTGFHCDIICVTGWTATPMQDVQVTIDWFISSQECVPRTYCVLNPQNP 190 Query: 118 --LNRYEMYRRSAFPKA---AIKRLIQSITG-----TSVSQNVVIAMSGISKVFVGEVVE 167 LNR+ + FP+ ++KR+ SI G +++ N+ A+ + + FVG++V Sbjct: 191 FVLNRW--MGKLTFPQGTSRSVKRMPLSIGGGAGAKSAILMNMPNAVLSMWRYFVGDLVF 248 Query: 168 EA 169 E Sbjct: 249 EV 250 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6MZL-P nrPDB 401 e-113 1BH8-B nrPDB 196 4e-51 Sequences not found previously or not previously below threshold: 2CU2-A nrPDB 29 1.2 2I1J-A nrPDB 28 2.8 3JAX-A nrPDB 27 4.6 1BR4-A nrPDB 27 4.6 1JFJ-A nrPDB 27 6.4 CONVERGED! >6MZL-P nrPDB Length = 211 Score = 401 bits (1031), Expect = e-113, Method: Composition-based stats. Identities = 211/211 (100%), Positives = 211/211 (100%) Query: 1 MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELES 60 MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELES Sbjct: 1 MDDAHESPSDKGGETGESDETAAVPGDPGATDTDGIPEETDGDADVDLKEAAAEEGELES 60 Query: 61 QDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNR 120 QDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNR Sbjct: 61 QDVSDLTTVEREDSSLLNPAAKKLKIDTKEKKEKKQKVDEDEIQKMQILVSSFSEEQLNR 120 Query: 121 YEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMP 180 YEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMP Sbjct: 121 YEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDVCEKWGEMP 180 Query: 181 PLQPKHMREAVRRLKSKGQIPNSKHKKIIFF 211 PLQPKHMREAVRRLKSKGQIPNSKHKKIIFF Sbjct: 181 PLQPKHMREAVRRLKSKGQIPNSKHKKIIFF 211 >1BH8-B nrPDB Length = 89 Score = 196 bits (499), Expect = 4e-51, Method: Composition-based stats. Identities = 89/89 (100%), Positives = 89/89 (100%) Query: 113 FSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDV 172 FSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDV Sbjct: 1 FSEEQLNRYEMYRRSAFPKAAIKRLIQSITGTSVSQNVVIAMSGISKVFVGEVVEEALDV 60 Query: 173 CEKWGEMPPLQPKHMREAVRRLKSKGQIP 201 CEKWGEMPPLQPKHMREAVRRLKSKGQIP Sbjct: 61 CEKWGEMPPLQPKHMREAVRRLKSKGQIP 89 >2CU2-A nrPDB Length = 337 Score = 29.3 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 15/47 (31%), Positives = 27/47 (57%), Gaps = 7/47 (14%) Query: 151 VIAMSGISKVFVGEVVEEALDVCEKWGEMPPLQPKHMREAVRRLKSK 197 V+A G+S + V +V +E L V + W + +RE V+RL+++ Sbjct: 297 VVATLGVSGLVVAKVGDEVLVVPKDWA-------REVREVVKRLEAQ 336 >2I1J-A nrPDB Length = 575 Score = 28.2 bits (61), Expect = 2.8, Method: Composition-based stats. Identities = 14/51 (27%), Positives = 29/51 (56%), Gaps = 2/51 (3%) Query: 59 ESQDVSDLTTVEREDSSLLNPAAKKLKID--TKEKKEKKQKVDEDEIQKMQ 107 ++ DV + RE+ +KL+++ +E+ EKKQ+ +D +++MQ Sbjct: 298 DTIDVQQMKAQAREEKLAKQAQREKLQLEIAARERAEKKQQEYQDRLRQMQ 348 >3JAX-A nrPDB Length = 974 Score = 27.4 bits (59), Expect = 4.6, Method: Composition-based stats. Identities = 12/44 (27%), Positives = 21/44 (47%) Query: 36 IPEETDGDADVDLKEAAAEEGELESQDVSDLTTVEREDSSLLNP 79 +P E G +KE +E +E Q+ T+ ++D +NP Sbjct: 37 VPSEKHGFEAASIKEEKGDEVTVELQENGKKVTLSKDDIQKMNP 80 >1BR4-A nrPDB Length = 820 Score = 27.4 bits (59), Expect = 4.6, Method: Composition-based stats. Identities = 12/44 (27%), Positives = 21/44 (47%) Query: 36 IPEETDGDADVDLKEAAAEEGELESQDVSDLTTVEREDSSLLNP 79 +P E G +KE +E +E Q+ T+ ++D +NP Sbjct: 36 VPSEKHGFEAASIKEEKGDEVTVELQENGKKVTLSKDDIQKMNP 79 >1JFJ-A nrPDB Length = 134 Score = 27.0 bits (58), Expect = 6.4, Method: Composition-based stats. Identities = 10/27 (37%), Positives = 17/27 (62%) Query: 39 ETDGDADVDLKEAAAEEGELESQDVSD 65 + DG+ ++D E A G ++ QD+SD Sbjct: 46 DADGNGEIDQNEFAKFYGSIQGQDLSD 72 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.307 0.127 0.341 Lambda K H 0.267 0.0395 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,234,025 Number of Sequences: 36641 Number of extensions: 327534 Number of successful extensions: 1077 Number of sequences better than 10.0: 30 Number of HSP's better than 10.0 without gapping: 30 Number of HSP's successfully gapped in prelim test: 45 Number of HSP's that attempted gapping in prelim test: 989 Number of HSP's gapped (non-prelim): 111 length of query: 211 length of database: 10,336,785 effective HSP length: 93 effective length of query: 118 effective length of database: 6,929,172 effective search space: 817642296 effective search space used: 817642296 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.6 bits) S2: 57 (26.6 bits)