BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q13889 (308 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5IY9-3 nrPDB 638 0.0 6GYM-4 nrPDB 137 8e-33 5OQM-4 nrPDB 122 2e-28 5NUS-A nrPDB 100 7e-22 5OQJ-6 nrPDB 33 0.13 3R7L-A nrPDB 28 3.8 3O07-A nrPDB 28 6.4 >5IY9-3 nrPDB Length = 308 Score = 638 bits (1646), Expect = 0.0, Method: Compositional matrix adjust. Identities = 308/308 (100%), Positives = 308/308 (100%) Query: 1 MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVI 60 MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVI Sbjct: 1 MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVI 60 Query: 61 ASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMT 120 ASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMT Sbjct: 61 ASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMT 120 Query: 121 KSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNV 180 KSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNV Sbjct: 121 KSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNV 180 Query: 181 IFAAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ 240 IFAAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ Sbjct: 181 IFAAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ 240 Query: 241 LILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAK 300 LILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAK Sbjct: 241 LILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAK 300 Query: 301 KKKLKVSA 308 KKKLKVSA Sbjct: 301 KKKLKVSA 308 >6GYM-4 nrPDB Length = 338 Score = 137 bits (344), Expect = 8e-33, Method: Compositional matrix adjust. Identities = 96/321 (29%), Positives = 156/321 (48%), Gaps = 23/321 (7%) Query: 5 EDELNLLVIVVDANP-IWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASH 63 E+ +LL ++++ P +W + ++ K ++A++V N+HL N +NK+AVIA++ Sbjct: 20 EESPSLLTVIIEIAPKLWTTFDEEGNEKGSIIKVLEALIVFLNAHLAFNSANKVAVIAAY 79 Query: 64 IQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDL--MTK 121 Q ++LYP Y + +E +VEEI L + K Sbjct: 80 SQGIKYLYPXXXXXXXXXXXXXXX--XXXXXXXXXXXYRRFRNVDETLVEEIYKLFELEK 137 Query: 122 SDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIK----AAEDSALQYMNF 177 I+ + LAG+++ L Y++R++KE +KSR+LV+ +++D QY+ Sbjct: 138 KQIEQNSQRSTLAGAMSAGLTYVNRISKE-SVTTSLKSRLLVLTCGSGSSKDEIFQYIPI 196 Query: 178 MNVIFAAQKQNILIDACVL--DSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQ 235 MN IF+A K ID + +S LQQ D T G+YL V L+QYL D Sbjct: 197 MNCIFSATKMKCPIDVVKIGGSKESTFLQQTTDATNGVYLHVESTEGLIQYLATAMFIDP 256 Query: 236 DQRSQLILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSP--ICTTCETAF---- 289 R ++ P VD+R +C+ ++ +G++CSVCL + P C C++ F Sbjct: 257 SLRPIIVKPNHGSVDFRTSCYLTGRVVAVGFICSVCLCVLSIIPPGNKCPACDSQFDEHV 316 Query: 290 --KISLPPV---LKAKKKKLK 305 K+ PV LKAKKK K Sbjct: 317 IAKLKRKPVVPRLKAKKKVTK 337 >5OQM-4 nrPDB Length = 333 Score = 122 bits (306), Expect = 2e-28, Method: Compositional matrix adjust. Identities = 91/321 (28%), Positives = 148/321 (46%), Gaps = 28/321 (8%) Query: 5 EDELNLLVIVVDANP-IWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASH 63 E+ +LL ++++ P +W + ++ K ++A++V N+HL N +NK+AVIA++ Sbjct: 20 EESPSLLTVIIEIAPKLWTTFDEEGNEKGSIIKVLEALIVFLNAHLAFNSANKVAVIAAY 79 Query: 64 IQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDL--MTK 121 Q ++LYP Y + +E +VEEI L + K Sbjct: 80 SQGIKYLYPXXXXXXXXXXXX-------XXXXXXXXXYRRFRNVDETLVEEIYKLFELEK 132 Query: 122 SDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIK----AAEDSALQYMNF 177 I+ + LAG+++ L Y++R++KE +KSR+LV+ +++D QY+ Sbjct: 133 KQIEQNSQRSTLAGAMSAGLTYVNRISKE-SVTTSLKSRLLVLTCGSGSSKDEIFQYIPI 191 Query: 178 MNVIFAAQKQNILIDACVL--DSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQ 235 MN IF+A K ID + +S LQQ D T G+YL V L+QYL D Sbjct: 192 MNCIFSATKMKCPIDVVKIGGSKESTFLQQTTDATNGVYLHVESTEGLIQYLATAMFIDP 251 Query: 236 DQRSQLILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSP--ICTTCETAF---- 289 +C+ ++ +G++CSVCL + P C C++ F Sbjct: 252 XXXXXXXXXXXXXXXXXTSCYLTGRVVAVGFICSVCLCVLSIIPPGNKCPACDSQFDEHV 311 Query: 290 --KISLPPV---LKAKKKKLK 305 K+ PV LKAKKK K Sbjct: 312 IAKLKRKPVVPRLKAKKKVTK 332 >5NUS-A nrPDB Length = 303 Score = 100 bits (249), Expect = 7e-22, Method: Compositional matrix adjust. Identities = 77/275 (28%), Positives = 119/275 (43%), Gaps = 67/275 (24%) Query: 5 EDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASHI 64 +D +L I++D NP W A LSK I +++ N+HL + SN++A+IASH Sbjct: 45 DDIPSLRTIIIDTNPRAWAALA---DVLPLSKAIANILIFVNAHLAFSNSNQVAIIASHT 101 Query: 65 QESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKD-----------GKYELLTSANEVIVE 113 + +LYP PPE PSGS GKY + ++ Sbjct: 102 NRAVWLYP--------------QPPEPLPSGSSSHDAAARKSATIGKYPQFAQIEKSLLS 147 Query: 114 EIKDLM---TKSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQE-------------- 156 I+ LM T SD+ T T ++G+L AL +I++ + + Sbjct: 148 SIRALMDDTTPSDLDT--TTTQISGALTLALAHINKTALSLTASNTAAAAVATGHSLTAG 205 Query: 157 --------------------MKSRILVIKAAEDSALQYMNFMNVIFAAQKQNILIDACVL 196 + +RIL+I ++ SA QY+ MN +FAA I ID L Sbjct: 206 SAASVAAKAASTSTSAGLAGLHARILIISVSDSSAAQYIPTMNAVFAAAHARIAIDTLAL 265 Query: 197 DSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVF 231 + L+QA IT G +++ + LLQYL++ F Sbjct: 266 RGSATFLEQASFITRGTFIRAAEPRGLLQYLMFGF 300 >5OQJ-6 nrPDB Length = 461 Score = 33.5 bits (75), Expect = 0.13, Method: Compositional matrix adjust. Identities = 14/43 (32%), Positives = 19/43 (44%) Query: 244 PPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCE 286 P + D C CH L+ GY C C S C+ +C C+ Sbjct: 325 PTRIFEDTPTFCSCHSKLVYGGYFCPNCHSKVCSLPTVCPCCD 367 >3R7L-A nrPDB Length = 304 Score = 28.5 bits (62), Expect = 3.8, Method: Compositional matrix adjust. Identities = 16/41 (39%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Query: 104 LTSANEVIVEEIKDLM-TKSDIKGQHTETLLAGSLAKALCY 143 LT+ +E+ EEIKDL+ T ++K T+ L G A L + Sbjct: 4 LTTMSELSTEEIKDLLQTAQELKSGKTDNQLTGKFAANLFF 44 >3O07-A nrPDB Length = 291 Score = 27.7 bits (60), Expect = 6.4, Method: Compositional matrix adjust. Identities = 20/77 (25%), Positives = 38/77 (49%), Gaps = 3/77 (3%) Query: 135 GSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNVIFAAQKQ--NILID 192 G +++A+ +I R+ +E+K Q++KS + K AE+ + +V+ + N Sbjct: 148 GDVSEAVKHIRRITEEIKACQQLKSEDDIAKVAEEMRVPVSLLKDVLEKGKLPVVNFAAG 207 Query: 193 ACVLDSDSGLLQQ-ACD 208 +D+ LL Q CD Sbjct: 208 GVATPADAALLMQLGCD 224 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5IY9-3 nrPDB 475 e-135 6GYM-4 nrPDB 353 8e-98 5OQM-4 nrPDB 318 2e-87 5NUS-A nrPDB 237 6e-63 Sequences not found previously or not previously below threshold: 5OQJ-6 nrPDB 40 0.002 4LAT-A nrPDB 28 6.0 2LCQ-A nrPDB 28 6.8 >5IY9-3 nrPDB Length = 308 Score = 475 bits (1223), Expect = e-135, Method: Composition-based stats. Identities = 308/308 (100%), Positives = 308/308 (100%) Query: 1 MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVI 60 MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVI Sbjct: 1 MVSDEDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVI 60 Query: 61 ASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMT 120 ASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMT Sbjct: 61 ASHIQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDLMT 120 Query: 121 KSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNV 180 KSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNV Sbjct: 121 KSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIKAAEDSALQYMNFMNV 180 Query: 181 IFAAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ 240 IFAAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ Sbjct: 181 IFAAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQDQRSQ 240 Query: 241 LILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAK 300 LILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAK Sbjct: 241 LILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCETAFKISLPPVLKAK 300 Query: 301 KKKLKVSA 308 KKKLKVSA Sbjct: 301 KKKLKVSA 308 >6GYM-4 nrPDB Length = 338 Score = 353 bits (905), Expect = 8e-98, Method: Composition-based stats. Identities = 96/321 (29%), Positives = 156/321 (48%), Gaps = 23/321 (7%) Query: 5 EDELNLLVIVVDANP-IWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASH 63 E+ +LL ++++ P +W + ++ K ++A++V N+HL N +NK+AVIA++ Sbjct: 20 EESPSLLTVIIEIAPKLWTTFDEEGNEKGSIIKVLEALIVFLNAHLAFNSANKVAVIAAY 79 Query: 64 IQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDL--MTK 121 Q ++LYP Y + +E +VEEI L + K Sbjct: 80 SQGIKYLYPXXXXXXXXXXXXXXXXXXXXXXXXX--XYRRFRNVDETLVEEIYKLFELEK 137 Query: 122 SDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIK----AAEDSALQYMNF 177 I+ + LAG+++ L Y++R++KE +KSR+LV+ +++D QY+ Sbjct: 138 KQIEQNSQRSTLAGAMSAGLTYVNRISKE-SVTTSLKSRLLVLTCGSGSSKDEIFQYIPI 196 Query: 178 MNVIFAAQKQNILIDACVL--DSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQ 235 MN IF+A K ID + +S LQQ D T G+YL V L+QYL D Sbjct: 197 MNCIFSATKMKCPIDVVKIGGSKESTFLQQTTDATNGVYLHVESTEGLIQYLATAMFIDP 256 Query: 236 DQRSQLILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSP--ICTTCETAF---- 289 R ++ P VD+R +C+ ++ +G++CSVCL + P C C++ F Sbjct: 257 SLRPIIVKPNHGSVDFRTSCYLTGRVVAVGFICSVCLCVLSIIPPGNKCPACDSQFDEHV 316 Query: 290 --KISLPPV---LKAKKKKLK 305 K+ PV LKAKKK K Sbjct: 317 IAKLKRKPVVPRLKAKKKVTK 337 >5OQM-4 nrPDB Length = 333 Score = 318 bits (815), Expect = 2e-87, Method: Composition-based stats. Identities = 91/321 (28%), Positives = 148/321 (46%), Gaps = 28/321 (8%) Query: 5 EDELNLLVIVVDANP-IWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASH 63 E+ +LL ++++ P +W + ++ K ++A++V N+HL N +NK+AVIA++ Sbjct: 20 EESPSLLTVIIEIAPKLWTTFDEEGNEKGSIIKVLEALIVFLNAHLAFNSANKVAVIAAY 79 Query: 64 IQESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKDGKYELLTSANEVIVEEIKDL--MTK 121 Q ++LYP Y + +E +VEEI L + K Sbjct: 80 SQGIKYLYPXXXXXXXXXXXXXXXX-------XXXXXYRRFRNVDETLVEEIYKLFELEK 132 Query: 122 SDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQEMKSRILVIK----AAEDSALQYMNF 177 I+ + LAG+++ L Y++R++KE +KSR+LV+ +++D QY+ Sbjct: 133 KQIEQNSQRSTLAGAMSAGLTYVNRISKE-SVTTSLKSRLLVLTCGSGSSKDEIFQYIPI 191 Query: 178 MNVIFAAQKQNILIDACVL--DSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPDQ 235 MN IF+A K ID + +S LQQ D T G+YL V L+QYL D Sbjct: 192 MNCIFSATKMKCPIDVVKIGGSKESTFLQQTTDATNGVYLHVESTEGLIQYLATAMFIDP 251 Query: 236 DQRSQLILPPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSP--ICTTCETAF---- 289 +C+ ++ +G++CSVCL + P C C++ F Sbjct: 252 XXXXXXXXXXXXXXXXXTSCYLTGRVVAVGFICSVCLCVLSIIPPGNKCPACDSQFDEHV 311 Query: 290 --KISLPPV---LKAKKKKLK 305 K+ PV LKAKKK K Sbjct: 312 IAKLKRKPVVPRLKAKKKVTK 332 >5NUS-A nrPDB Length = 303 Score = 237 bits (604), Expect = 6e-63, Method: Composition-based stats. Identities = 77/275 (28%), Positives = 119/275 (43%), Gaps = 67/275 (24%) Query: 5 EDELNLLVIVVDANPIWWGKQALKESQFTLSKCIDAVMVLGNSHLFMNRSNKLAVIASHI 64 +D +L I++D NP W A LSK I +++ N+HL + SN++A+IASH Sbjct: 45 DDIPSLRTIIIDTNPRAWAALA---DVLPLSKAIANILIFVNAHLAFSNSNQVAIIASHT 101 Query: 65 QESRFLYPGKNGRLGDFFGDPGNPPEFNPSGSKD-----------GKYELLTSANEVIVE 113 + +LYP PPE PSGS GKY + ++ Sbjct: 102 NRAVWLYP--------------QPPEPLPSGSSSHDAAARKSATIGKYPQFAQIEKSLLS 147 Query: 114 EIKDLM---TKSDIKGQHTETLLAGSLAKALCYIHRMNKEVKDNQE-------------- 156 I+ LM T SD+ T T ++G+L AL +I++ + + Sbjct: 148 SIRALMDDTTPSDLDT--TTTQISGALTLALAHINKTALSLTASNTAAAAVATGHSLTAG 205 Query: 157 --------------------MKSRILVIKAAEDSALQYMNFMNVIFAAQKQNILIDACVL 196 + +RIL+I ++ SA QY+ MN +FAA I ID L Sbjct: 206 SAASVAAKAASTSTSAGLAGLHARILIISVSDSSAAQYIPTMNAVFAAAHARIAIDTLAL 265 Query: 197 DSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVF 231 + L+QA IT G +++ + LLQYL++ F Sbjct: 266 RGSATFLEQASFITRGTFIRAAEPRGLLQYLMFGF 300 >5OQJ-6 nrPDB Length = 461 Score = 39.7 bits (91), Expect = 0.002, Method: Composition-based stats. Identities = 14/43 (32%), Positives = 19/43 (44%) Query: 244 PPPVHVDYRAACFCHRNLIEIGYVCSVCLSIFCNFSPICTTCE 286 P + D C CH L+ GY C C S C+ +C C+ Sbjct: 325 PTRIFEDTPTFCSCHSKLVYGGYFCPNCHSKVCSLPTVCPCCD 367 >4LAT-A nrPDB Length = 265 Score = 27.7 bits (60), Expect = 6.0, Method: Composition-based stats. Identities = 11/52 (21%), Positives = 20/52 (38%) Query: 183 AAQKQNILIDACVLDSDSGLLQQACDITGGLYLKVPQMPSLLQYLLWVFLPD 234 A D +++ S + Q D G + V + P + YL ++ D Sbjct: 129 AGSGSRATFDTVIMEGQSAMQSQEQDSNGAVKSIVSKSPGAISYLSLTYIDD 180 >2LCQ-A nrPDB Length = 165 Score = 27.7 bits (60), Expect = 6.8, Method: Composition-based stats. Identities = 11/43 (25%), Positives = 18/43 (41%), Gaps = 2/43 (4%) Query: 251 YRAACFCHRNLIEIGYVCSVCLSIFCNFSP--ICTTCETAFKI 291 +R + +I+ YVC C F P +C C + K+ Sbjct: 118 FRTLKRGIKKVIKWRYVCIGCGRKFSTLPPGGVCPDCGSKVKL 160 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.322 0.131 0.347 Lambda K H 0.267 0.0397 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,946,116 Number of Sequences: 36641 Number of extensions: 417388 Number of successful extensions: 1103 Number of sequences better than 10.0: 15 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 1058 Number of HSP's gapped (non-prelim): 31 length of query: 308 length of database: 10,336,785 effective HSP length: 97 effective length of query: 211 effective length of database: 6,782,608 effective search space: 1431130288 effective search space used: 1431130288 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 59 (27.4 bits)