BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q13432 (240 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4GOK-G nrPDB 492 e-140 3RBQ-A nrPDB 386 e-108 5T4X-A nrPDB 47 8e-06 1KSH-B nrPDB 45 4e-05 2PFM-A nrPDB 29 1.4 2X75-A nrPDB 29 1.9 1F1O-A nrPDB 28 2.9 6CC2-A nrPDB 28 4.8 4LV5-B nrPDB 27 6.1 >4GOK-G nrPDB Length = 240 Score = 492 bits (1267), Expect = e-140, Method: Compositional matrix adjust. Identities = 240/240 (100%), Positives = 240/240 (100%) Query: 1 MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPI 60 MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPI Sbjct: 1 MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPI 60 Query: 61 GPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRD 120 GPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRD Sbjct: 61 GPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRD 120 Query: 121 LDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFG 180 LDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFG Sbjct: 121 LDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFG 180 Query: 181 FCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP 240 FCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP Sbjct: 181 FCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP 240 >3RBQ-A nrPDB Length = 195 Score = 386 bits (991), Expect = e-108, Method: Compositional matrix adjust. Identities = 185/186 (99%), Positives = 185/186 (99%) Query: 55 QRKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERL 114 RKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERL Sbjct: 10 HRKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERL 69 Query: 115 PINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKS 174 PINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKS Sbjct: 70 PINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKS 129 Query: 175 FDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADY 234 FDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADY Sbjct: 130 FDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADY 189 Query: 235 SYSGTP 240 SYSGTP Sbjct: 190 SYSGTP 195 >5T4X-A nrPDB Length = 170 Score = 47.0 bits (110), Expect = 8e-06, Method: Compositional matrix adjust. Identities = 27/99 (27%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 138 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 197 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 74 LKCKAVSRELNFSSAEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 132 Query: 198 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 236 P S+ + + ++ ++ +F DD LV +K Y Sbjct: 133 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSKVRLFY 169 >1KSH-B nrPDB Length = 152 Score = 44.7 bits (104), Expect = 4e-05, Method: Compositional matrix adjust. Identities = 26/99 (26%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 138 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 197 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 56 LKCKAVSRELNFSSTEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 114 Query: 198 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 236 P S+ + + ++ ++ +F DD LV ++ Y Sbjct: 115 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSRVRLFY 151 >2PFM-A nrPDB Length = 444 Score = 29.3 bits (64), Expect = 1.4, Method: Compositional matrix adjust. Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Query: 33 AESESGSESEPDAGPGPRPGPLQRKQPIGPEDVLGLQRITGDYLCSPEENI 83 +E+ E+ G P ++ PIG E++ GL R+ Y+ + EN+ Sbjct: 257 SETREVEEAFAKGQKGSSAMP-HKRNPIGSENMTGLARVIRGYMMTAYENV 306 >2X75-A nrPDB Length = 431 Score = 28.9 bits (63), Expect = 1.9, Method: Compositional matrix adjust. Identities = 9/29 (31%), Positives = 18/29 (62%) Query: 55 QRKQPIGPEDVLGLQRITGDYLCSPEENI 83 ++ PIG E++ G+ R+ Y+ + EN+ Sbjct: 267 HKRNPIGSENITGISRVIRGYITTAYENV 295 >1F1O-A nrPDB Length = 431 Score = 28.5 bits (62), Expect = 2.9, Method: Compositional matrix adjust. Identities = 9/29 (31%), Positives = 18/29 (62%) Query: 55 QRKQPIGPEDVLGLQRITGDYLCSPEENI 83 ++ PIG E++ G+ R+ Y+ + EN+ Sbjct: 267 HKRNPIGSENMTGMARVIRGYMMTAYENV 295 >6CC2-A nrPDB Length = 543 Score = 27.7 bits (60), Expect = 4.8, Method: Compositional matrix adjust. Identities = 17/54 (31%), Positives = 25/54 (46%), Gaps = 15/54 (27%) Query: 175 FDFHFGFCIPSSKNTCE---------------HIYDFPPLSEELISEMIRHPYE 213 +DF F CI S+KN E + Y F +S+EL+S + PY+ Sbjct: 393 YDFGFAKCIESNKNLVETGIILLLSGSCFNEANKYRFCSVSDELLSIRFKTPYK 446 >4LV5-B nrPDB Length = 423 Score = 27.3 bits (59), Expect = 6.1, Method: Compositional matrix adjust. Identities = 12/25 (48%), Positives = 18/25 (72%), Gaps = 2/25 (8%) Query: 183 IPSSKNTCEHIYDFPPLSEELISEM 207 + S+KN C YDFP L ++LIS++ Sbjct: 239 LLSNKNVCH--YDFPVLMDKLISDL 261 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4GOK-G nrPDB 462 e-131 3RBQ-A nrPDB 374 e-104 5T4X-A nrPDB 156 6e-39 1KSH-B nrPDB 154 3e-38 Sequences not found previously or not previously below threshold: 5L3D-A nrPDB 35 0.035 2X75-A nrPDB 33 0.12 2PFM-A nrPDB 33 0.13 1F1O-A nrPDB 32 0.23 5EF4-A nrPDB 32 0.36 1IGN-A nrPDB 31 0.59 6CGR-k nrPDB 30 0.68 1ZCM-A nrPDB 30 1.0 3MAJ-A nrPDB 28 3.4 1JLK-A nrPDB 28 3.9 CONVERGED! >4GOK-G nrPDB Length = 240 Score = 462 bits (1190), Expect = e-131, Method: Composition-based stats. Identities = 240/240 (100%), Positives = 240/240 (100%) Query: 1 MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPI 60 MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPI Sbjct: 1 MKVKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPI 60 Query: 61 GPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRD 120 GPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRD Sbjct: 61 GPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRD 120 Query: 121 LDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFG 180 LDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFG Sbjct: 121 LDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFG 180 Query: 181 FCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP 240 FCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP Sbjct: 181 FCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSYSGTP 240 >3RBQ-A nrPDB Length = 195 Score = 374 bits (960), Expect = e-104, Method: Composition-based stats. Identities = 185/186 (99%), Positives = 185/186 (99%) Query: 55 QRKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERL 114 RKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERL Sbjct: 10 HRKQPIGPEDVLGLQRITGDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERL 69 Query: 115 PINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKS 174 PINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKS Sbjct: 70 PINRRDLDPNAGRFVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKS 129 Query: 175 FDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADY 234 FDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADY Sbjct: 130 FDFHFGFCIPSSKNTCEHIYDFPPLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADY 189 Query: 235 SYSGTP 240 SYSGTP Sbjct: 190 SYSGTP 195 >5T4X-A nrPDB Length = 170 Score = 156 bits (395), Expect = 6e-39, Method: Composition-based stats. Identities = 27/99 (27%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 138 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 197 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 74 LKCKAVSRELNFSSAEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 132 Query: 198 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 236 P S+ + + ++ ++ +F DD LV +K Y Sbjct: 133 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSKVRLFY 169 >1KSH-B nrPDB Length = 152 Score = 154 bits (389), Expect = 3e-38, Method: Composition-based stats. Identities = 26/99 (26%), Positives = 53/99 (53%), Gaps = 3/99 (3%) Query: 138 LRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDFHFGFCIPSSKNTCEHIYDFP 197 L+ + V + F+ ++ + FR+ ++ YF+ Q L+ + F FGF IP+S NT + + + Sbjct: 56 LKCKAVSRELNFSSTEQ-MEKFRLEQKVYFKGQCLEEWFFEFGFVIPNSTNTWQSLIEAA 114 Query: 198 PLSEELISEMIRHPYETQSDSFYFVDDRLVMHNKADYSY 236 P S+ + + ++ ++ +F DD LV ++ Y Sbjct: 115 PESQMMPASVLTGNVIIETK--FFDDDLLVSTSRVRLFY 151 >5L3D-A nrPDB Length = 852 Score = 34.6 bits (78), Expect = 0.035, Method: Composition-based stats. Identities = 15/46 (32%), Positives = 19/46 (41%) Query: 8 GGAGTATESAPGPSGQSVAPIPQPPAESESGSESEPDAGPGPRPGP 53 G E PG G+ +PP S G +EP GP+ GP Sbjct: 42 AGLSGPAEVGPGAVGERTPRKKEPPRASPPGGLAEPPGSAGPQAGP 87 >2X75-A nrPDB Length = 431 Score = 33.1 bits (74), Expect = 0.12, Method: Composition-based stats. Identities = 13/51 (25%), Positives = 25/51 (49%), Gaps = 11/51 (21%) Query: 33 AESESGSESEPDAGPGPRPGPLQRKQPIGPEDVLGLQRITGDYLCSPEENI 83 A+ + GS + P ++ PIG E++ G+ R+ Y+ + EN+ Sbjct: 256 AKGQKGSSAMP-----------HKRNPIGSENITGISRVIRGYITTAYENV 295 >2PFM-A nrPDB Length = 444 Score = 32.7 bits (73), Expect = 0.13, Method: Composition-based stats. Identities = 14/51 (27%), Positives = 25/51 (49%), Gaps = 11/51 (21%) Query: 33 AESESGSESEPDAGPGPRPGPLQRKQPIGPEDVLGLQRITGDYLCSPEENI 83 A+ + GS + P ++ PIG E++ GL R+ Y+ + EN+ Sbjct: 267 AKGQKGSSAMP-----------HKRNPIGSENMTGLARVIRGYMMTAYENV 306 >1F1O-A nrPDB Length = 431 Score = 31.9 bits (71), Expect = 0.23, Method: Composition-based stats. Identities = 13/51 (25%), Positives = 25/51 (49%), Gaps = 11/51 (21%) Query: 33 AESESGSESEPDAGPGPRPGPLQRKQPIGPEDVLGLQRITGDYLCSPEENI 83 A+ + GS + P ++ PIG E++ G+ R+ Y+ + EN+ Sbjct: 256 AKGQKGSSAMP-----------HKRNPIGSENMTGMARVIRGYMMTAYENV 295 >5EF4-A nrPDB Length = 385 Score = 31.5 bits (70), Expect = 0.36, Method: Composition-based stats. Identities = 18/53 (33%), Positives = 24/53 (45%), Gaps = 3/53 (5%) Query: 11 GTATESAPGPSGQSVAPI---PQPPAESESGSESEPDAGPGPRPGPLQRKQPI 60 G E G + S PI P PP + +G + +PDA P+ QR Q I Sbjct: 326 GARKEGLCGVAMHSSFPIMNDPNPPKDDPNGPKDDPDAPKDPKFKTTQRLQGI 378 >1IGN-A nrPDB Length = 246 Score = 30.8 bits (68), Expect = 0.59, Method: Composition-based stats. Identities = 27/123 (21%), Positives = 45/123 (36%), Gaps = 19/123 (15%) Query: 75 YLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPINR-------------RDL 121 YL E +Y++D +RD D + ++ P + + + RDL Sbjct: 58 YLSKRLEYVYEVDKFGKLVRDDDGNLIKTKVLPPSIKRKFSADEDYTLAIAVKKQFYRDL 117 Query: 122 ---DPNAGR-FVRYQFTPAFLRLRQVGATVEFTVGDKPVNNFRMIERHYFRNQLLKSFDF 177 DP+ GR + + TP + R + G +P NF R + + F Sbjct: 118 FQIDPDTGRSLITDEDTPTAIARRNMTMDPNHVPGSEP--NFAAYRTQSRRGPIAREFFK 175 Query: 178 HFG 180 HF Sbjct: 176 HFA 178 >6CGR-k nrPDB Length = 703 Score = 30.4 bits (67), Expect = 0.68, Method: Composition-based stats. Identities = 15/45 (33%), Positives = 22/45 (48%), Gaps = 7/45 (15%) Query: 22 GQSVAPIPQPPAESESGSESEPDAGPGPRPGPLQRKQPIGPEDVL 66 G+S +P PQP A +G+ ++P P PI PE+ L Sbjct: 201 GESASPRPQPLARPHAGAPADPPIVGASDP-------PISPEEQL 238 >1ZCM-A nrPDB Length = 321 Score = 30.0 bits (66), Expect = 1.0, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 24/41 (58%), Gaps = 2/41 (4%) Query: 87 DFVRFKIRDMDSGTVLFEIKKPPVSERLPINRRDLDPNAGR 127 D+ + ++R + SGT+ + PPV + L +DL PN+ + Sbjct: 9 DYEQLRVRCLQSGTLFRDEAFPPVPQSL--GYKDLGPNSSK 47 >3MAJ-A nrPDB Length = 382 Score = 28.1 bits (61), Expect = 3.4, Method: Composition-based stats. Identities = 20/71 (28%), Positives = 31/71 (43%), Gaps = 1/71 (1%) Query: 3 VKKGGGGAGTATESAPGPSGQSVAPIPQPPAESESGS-ESEPDAGPGPRPGPLQRKQPIG 61 +K+G +A++ + PI P E E E EPD G R L P+G Sbjct: 284 IKQGATLITSASDIVEAVASILERPIELPGREPEHAPPEGEPDTGDRTRILALLGPSPVG 343 Query: 62 PEDVLGLQRIT 72 +D++ L I+ Sbjct: 344 IDDLIRLSGIS 354 >1JLK-A nrPDB Length = 147 Score = 28.1 bits (61), Expect = 3.9, Method: Composition-based stats. Identities = 15/44 (34%), Positives = 23/44 (52%), Gaps = 3/44 (6%) Query: 73 GDYLCSPEENIYKIDFVRFKIRDMDSGTVLFEIKKPPVSERLPI 116 G+Y SP N+ +D + D VL EIK+ P +R+P+ Sbjct: 54 GEYENSPRPNLILLDL---NLPKKDGREVLAEIKQNPDLKRIPV 94 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.308 0.137 0.409 Lambda K H 0.267 0.0404 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,501,318 Number of Sequences: 36641 Number of extensions: 473019 Number of successful extensions: 1279 Number of sequences better than 10.0: 24 Number of HSP's better than 10.0 without gapping: 24 Number of HSP's successfully gapped in prelim test: 29 Number of HSP's that attempted gapping in prelim test: 1143 Number of HSP's gapped (non-prelim): 119 length of query: 240 length of database: 10,336,785 effective HSP length: 94 effective length of query: 146 effective length of database: 6,892,531 effective search space: 1006309526 effective search space used: 1006309526 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 57 (26.6 bits)