BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q12980 (569 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6CET-M nrPDB 1168 0.0 3S6L-A nrPDB 31 1.4 5JSR-A nrPDB 29 6.3 3K4Z-A nrPDB 28 7.8 >6CET-M nrPDB Length = 569 Score = 1168 bits (3022), Expect = 0.0, Method: Compositional matrix adjust. Identities = 569/569 (100%), Positives = 569/569 (100%) Query: 1 MRDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTSKPRSRYAASNTGDHADEQDG 60 MRDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTSKPRSRYAASNTGDHADEQDG Sbjct: 1 MRDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTSKPRSRYAASNTGDHADEQDG 60 Query: 61 DSRFSDVILATILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQISKTDPSPKREAPT 120 DSRFSDVILATILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQISKTDPSPKREAPT Sbjct: 61 DSRFSDVILATILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQISKTDPSPKREAPT 120 Query: 121 MILFNVVFALRANADPSVINCLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAM 180 MILFNVVFALRANADPSVINCLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAM Sbjct: 121 MILFNVVFALRANADPSVINCLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAM 180 Query: 181 ADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAAS 240 ADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAAS Sbjct: 181 ADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAAS 240 Query: 241 SLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQ 300 SLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQ Sbjct: 241 SLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQ 300 Query: 301 DADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHD 360 DADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHD Sbjct: 301 DADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHD 360 Query: 361 LPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEE 420 LPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEE Sbjct: 361 LPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEE 420 Query: 421 PRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQ 480 PRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQ Sbjct: 421 PRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQ 480 Query: 481 RMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQL 540 RMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQL Sbjct: 481 RMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQL 540 Query: 541 LMLFDKFRSVLVVTTHEDPVIAVFQALLP 569 LMLFDKFRSVLVVTTHEDPVIAVFQALLP Sbjct: 541 LMLFDKFRSVLVVTTHEDPVIAVFQALLP 569 >3S6L-A nrPDB Length = 178 Score = 31.2 bits (69), Expect = 1.4, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Query: 429 PFTARVGGRSL-STPNALSFGSPTSSDDMTLTSPSMDNSSA 468 P +A +G S+ S PN++SFGS +T +P +D + A Sbjct: 119 PNSAAIGANSVASAPNSVSFGSRGHERRLTNVAPGIDGTDA 159 >5JSR-A nrPDB Length = 201 Score = 28.9 bits (63), Expect = 6.3, Method: Compositional matrix adjust. Identities = 23/74 (31%), Positives = 31/74 (41%), Gaps = 5/74 (6%) Query: 248 IERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALV--RVIKTTSAVKNLQQLAQDADLA 305 +E L+A R H L E G P D + + R I T V+ L Q Sbjct: 4 LENVLEAARHLHQTLPALSE---FGNWPTDLTATGLQPRAIPATPLVQALDQPGSPRTTG 60 Query: 306 LLQVFQLAAHLVYW 319 L+Q + AAHL +W Sbjct: 61 LVQAIRSAAHLAHW 74 >3K4Z-A nrPDB Length = 289 Score = 28.5 bits (62), Expect = 7.8, Method: Compositional matrix adjust. Identities = 21/84 (25%), Positives = 35/84 (41%), Gaps = 11/84 (13%) Query: 422 RPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQR 481 +P +D FT +GG +TP + DD++L P +LP D +NQ Sbjct: 139 KPTDDTCEFTFHLGGELAATPPYTVY-----LDDVSLYDPEYTKPVEYILPQPDVRVNQ- 192 Query: 482 MTENLLASLSEHERAAILSVPAAQ 505 + L E ++ A + + Q Sbjct: 193 -----VGYLPEGKKVATVVCNSTQ 211 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6CET-M nrPDB 1179 0.0 Sequences not found previously or not previously below threshold: 5JSR-A nrPDB 31 1.1 3S6L-A nrPDB 31 1.6 3K4Z-A nrPDB 30 3.2 CONVERGED! >6CET-M nrPDB Length = 569 Score = 1179 bits (3051), Expect = 0.0, Method: Composition-based stats. Identities = 569/569 (100%), Positives = 569/569 (100%) Query: 1 MRDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTSKPRSRYAASNTGDHADEQDG 60 MRDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTSKPRSRYAASNTGDHADEQDG Sbjct: 1 MRDNTSPISVILVSSGSRGNKLLFRYPFQRSQEHPASQTSKPRSRYAASNTGDHADEQDG 60 Query: 61 DSRFSDVILATILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQISKTDPSPKREAPT 120 DSRFSDVILATILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQISKTDPSPKREAPT Sbjct: 61 DSRFSDVILATILATKSEMCGQKFELKIDNVRFVGHPTLLQHALGQISKTDPSPKREAPT 120 Query: 121 MILFNVVFALRANADPSVINCLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAM 180 MILFNVVFALRANADPSVINCLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAM Sbjct: 121 MILFNVVFALRANADPSVINCLHNLSRRIATVLQHEERRCQYLTREAKLILALQDEVSAM 180 Query: 181 ADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAAS 240 ADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAAS Sbjct: 181 ADGNEGPQSPFHHILPKCKLARDLKEAYDSLCTSGVVRLHINSWLEVSFCLPHKIHYAAS 240 Query: 241 SLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQ 300 SLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQ Sbjct: 241 SLIPPEAIERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALVRVIKTTSAVKNLQQLAQ 300 Query: 301 DADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHD 360 DADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHD Sbjct: 301 DADLALLQVFQLAAHLVYWGKAIIIYPLCENNVYMLSPNASVCLYSPLAEQFSHQFPSHD 360 Query: 361 LPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEE 420 LPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEE Sbjct: 361 LPSVLAKFSLPVSLSEFRNPLAPAVQETQLIQMVVWMLQRRLLIQLHTYVCLMASPSEEE 420 Query: 421 PRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQ 480 PRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQ Sbjct: 421 PRPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQ 480 Query: 481 RMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQL 540 RMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQL Sbjct: 481 RMTENLLASLSEHERAAILSVPAAQNPEDLRMFARLLHYFRGRHHLEEIMYNENTRRSQL 540 Query: 541 LMLFDKFRSVLVVTTHEDPVIAVFQALLP 569 LMLFDKFRSVLVVTTHEDPVIAVFQALLP Sbjct: 541 LMLFDKFRSVLVVTTHEDPVIAVFQALLP 569 >5JSR-A nrPDB Length = 201 Score = 31.2 bits (69), Expect = 1.1, Method: Composition-based stats. Identities = 23/76 (30%), Positives = 32/76 (42%), Gaps = 5/76 (6%) Query: 248 IERSLKAIRPYHALLLLSDEKSLLGELPIDCSPALV--RVIKTTSAVKNLQQLAQDADLA 305 +E L+A R H L E G P D + + R I T V+ L Q Sbjct: 4 LENVLEAARHLHQTLPALSE---FGNWPTDLTATGLQPRAIPATPLVQALDQPGSPRTTG 60 Query: 306 LLQVFQLAAHLVYWGK 321 L+Q + AAHL +W + Sbjct: 61 LVQAIRSAAHLAHWKR 76 >3S6L-A nrPDB Length = 178 Score = 30.8 bits (68), Expect = 1.6, Method: Composition-based stats. Identities = 15/41 (36%), Positives = 24/41 (58%), Gaps = 1/41 (2%) Query: 429 PFTARVGGRSL-STPNALSFGSPTSSDDMTLTSPSMDNSSA 468 P +A +G S+ S PN++SFGS +T +P +D + A Sbjct: 119 PNSAAIGANSVASAPNSVSFGSRGHERRLTNVAPGIDGTDA 159 >3K4Z-A nrPDB Length = 289 Score = 29.7 bits (65), Expect = 3.2, Method: Composition-based stats. Identities = 17/59 (28%), Positives = 26/59 (44%), Gaps = 5/59 (8%) Query: 422 RPREDDVPFTARVGGRSLSTPNALSFGSPTSSDDMTLTSPSMDNSSAELLPSGDSPLNQ 480 +P +D FT +GG +TP + DD++L P +LP D +NQ Sbjct: 139 KPTDDTCEFTFHLGGELAATPPYTVY-----LDDVSLYDPEYTKPVEYILPQPDVRVNQ 192 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.133 0.387 Lambda K H 0.267 0.0408 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 22,068,912 Number of Sequences: 36641 Number of extensions: 851375 Number of successful extensions: 1892 Number of sequences better than 10.0: 4 Number of HSP's better than 10.0 without gapping: 2 Number of HSP's successfully gapped in prelim test: 6 Number of HSP's that attempted gapping in prelim test: 1890 Number of HSP's gapped (non-prelim): 8 length of query: 569 length of database: 10,336,785 effective HSP length: 102 effective length of query: 467 effective length of database: 6,599,403 effective search space: 3081921201 effective search space used: 3081921201 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.8 bits) S2: 62 (28.5 bits)