BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q12517 (231 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 1Q67-A nrPDB 475 e-135 5LON-B nrPDB 239 9e-64 2LYD-A nrPDB 36 0.012 4B6H-A nrPDB 31 0.35 5KQ4-D nrPDB 30 1.2 4N81-A nrPDB 29 1.4 3QZ4-A nrPDB 28 3.1 2DYB-A nrPDB 28 4.3 >1Q67-A nrPDB Length = 231 Score = 475 bits (1222), Expect = e-135, Method: Compositional matrix adjust. Identities = 231/231 (100%), Positives = 231/231 (100%) Query: 1 MTGAATAAENSATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLE 60 MTGAATAAENSATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLE Sbjct: 1 MTGAATAAENSATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLE 60 Query: 61 YQGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKS 120 YQGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKS Sbjct: 61 YQGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKS 120 Query: 121 NDSLTYNCGKTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVFNAEEDTLNPLE 180 NDSLTYNCGKTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVFNAEEDTLNPLE Sbjct: 121 NDSLTYNCGKTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVFNAEEDTLNPLE 180 Query: 181 CMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA 231 CMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA Sbjct: 181 CMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA 231 >5LON-B nrPDB Length = 188 Score = 239 bits (609), Expect = 9e-64, Method: Compositional matrix adjust. Identities = 132/224 (58%), Positives = 148/224 (66%), Gaps = 40/224 (17%) Query: 11 SATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVLAIYLR 70 S LE YRKALNFNVI RYDPKIKQLLFHTPHA++YKW D WNKLEYQGVLAIYLR Sbjct: 2 STETLEIYRKALNFNVIARYDPKIKQLLFHTPHATVYKWG--DDNWNKLEYQGVLAIYLR 59 Query: 71 DVSQNTNLLP-VSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKSNDSLTYNCG 129 DV +LP VS + I Q+ +N V Sbjct: 60 DVGDKEAILPEVSSYDDTITGQQSEANTPHV----------------------------- 90 Query: 130 KTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVF--NAEEDTLNPLECMGVEVK 187 L+G DIYNYGLII+NRINPDNFS+ I PNSV+NKRK+F N EE+ LE M VEV+ Sbjct: 91 --LTGHDIYNYGLIIMNRINPDNFSLAIAPNSVLNKRKLFAPNREEE----LEPMKVEVR 144 Query: 188 DELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA 231 D+LV+IK LK EVYGIW+HT DRQNIYELIKYLLENEP DSF Sbjct: 145 DDLVMIKTLKKEVYGIWVHTPEDRQNIYELIKYLLENEPTDSFT 188 >2LYD-A nrPDB Length = 134 Score = 36.2 bits (82), Expect = 0.012, Method: Compositional matrix adjust. Identities = 14/51 (27%), Positives = 28/51 (54%) Query: 22 LNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVLAIYLRDV 72 +N I + DP K+++ + H + Y ++ ++EW K + +G IY R+ Sbjct: 16 MNLAAIKKIDPYAKEIVDSSSHVAFYTFNSSQNEWEKTDVEGAFFIYHRNA 66 >4B6H-A nrPDB Length = 134 Score = 31.2 bits (69), Expect = 0.35, Method: Compositional matrix adjust. Identities = 14/54 (25%), Positives = 28/54 (51%) Query: 20 KALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVLAIYLRDVS 73 + ++ + ++DP I + T +LY + K ++W K + +G L +Y R S Sbjct: 13 QEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSAS 66 >5KQ4-D nrPDB Length = 130 Score = 29.6 bits (65), Expect = 1.2, Method: Compositional matrix adjust. Identities = 12/49 (24%), Positives = 26/49 (53%) Query: 19 RKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVLAI 67 R A+N V+ + P+I+ ++ H ++Y++D +W K +G + Sbjct: 11 RNAVNLQVLKFHYPEIESIIDIASHVAVYQFDVGSQKWLKTSIEGTFFL 59 >4N81-A nrPDB Length = 259 Score = 29.3 bits (64), Expect = 1.4, Method: Compositional matrix adjust. Identities = 12/28 (42%), Positives = 15/28 (53%) Query: 122 DSLTYNCGKTLSGKDIYNYGLIILNRIN 149 D + C TL G D YNYGL+ L + Sbjct: 172 DKIRLKCRDTLLGGDCYNYGLLSLGHCD 199 >3QZ4-A nrPDB Length = 311 Score = 28.1 bits (61), Expect = 3.1, Method: Compositional matrix adjust. Identities = 13/39 (33%), Positives = 21/39 (53%), Gaps = 4/39 (10%) Query: 164 NKRKVFNAEEDTLNPLECMGVEVKDELVIIKNLKHEVYG 202 N V+ + L P+E K+ +V+I+N K E+YG Sbjct: 220 NYHVVYGTAQSPLGPIEV----AKEPIVLIQNPKEEIYG 254 >2DYB-A nrPDB Length = 341 Score = 27.7 bits (60), Expect = 4.3, Method: Compositional matrix adjust. Identities = 22/64 (34%), Positives = 30/64 (46%), Gaps = 8/64 (12%) Query: 142 LIILNRINPDNF------SMGIVPNSVVNKRKVFNAEEDTLNPLECMGVEVKDELVIIKN 195 + +L+RIN D + GI P S V K F E+D N L C E D + IK+ Sbjct: 199 IFLLSRINKDWLEGTVRGATGIFPLSFVKILKDFPEEDDPTNWLRCYYYE--DTISTIKD 256 Query: 196 LKHE 199 + E Sbjct: 257 IAVE 260 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 1Q67-A nrPDB 397 e-111 5LON-B nrPDB 303 6e-83 Sequences not found previously or not previously below threshold: 2LYD-A nrPDB 36 0.013 4CFF-A nrPDB 34 0.041 5EZV-A nrPDB 34 0.076 4HZS-A nrPDB 32 0.17 1U54-B nrPDB 32 0.17 4B6H-A nrPDB 31 0.53 5KQ4-D nrPDB 30 0.66 1KZH-A nrPDB 28 3.0 3O04-A nrPDB 28 3.8 4N81-A nrPDB 27 5.7 CONVERGED! >1Q67-A nrPDB Length = 231 Score = 397 bits (1021), Expect = e-111, Method: Composition-based stats. Identities = 231/231 (100%), Positives = 231/231 (100%) Query: 1 MTGAATAAENSATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLE 60 MTGAATAAENSATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLE Sbjct: 1 MTGAATAAENSATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLE 60 Query: 61 YQGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKS 120 YQGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKS Sbjct: 61 YQGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKS 120 Query: 121 NDSLTYNCGKTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVFNAEEDTLNPLE 180 NDSLTYNCGKTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVFNAEEDTLNPLE Sbjct: 121 NDSLTYNCGKTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVFNAEEDTLNPLE 180 Query: 181 CMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA 231 CMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA Sbjct: 181 CMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA 231 >5LON-B nrPDB Length = 188 Score = 303 bits (775), Expect = 6e-83, Method: Composition-based stats. Identities = 132/224 (58%), Positives = 148/224 (66%), Gaps = 40/224 (17%) Query: 11 SATQLEFYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVLAIYLR 70 S LE YRKALNFNVI RYDPKIKQLLFHTPHA++YKW D WNKLEYQGVLAIYLR Sbjct: 2 STETLEIYRKALNFNVIARYDPKIKQLLFHTPHATVYKWG--DDNWNKLEYQGVLAIYLR 59 Query: 71 DVSQNTNLLP-VSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKSNDSLTYNCG 129 DV +LP VS + I Q+ +N V Sbjct: 60 DVGDKEAILPEVSSYDDTITGQQSEANTPHV----------------------------- 90 Query: 130 KTLSGKDIYNYGLIILNRINPDNFSMGIVPNSVVNKRKVF--NAEEDTLNPLECMGVEVK 187 L+G DIYNYGLII+NRINPDNFS+ I PNSV+NKRK+F N EE+ LE M VEV+ Sbjct: 91 --LTGHDIYNYGLIIMNRINPDNFSLAIAPNSVLNKRKLFAPNREEE----LEPMKVEVR 144 Query: 188 DELVIIKNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSFA 231 D+LV+IK LK EVYGIW+HT DRQNIYELIKYLLENEP DSF Sbjct: 145 DDLVMIKTLKKEVYGIWVHTPEDRQNIYELIKYLLENEPTDSFT 188 >2LYD-A nrPDB Length = 134 Score = 36.3 bits (82), Expect = 0.013, Method: Composition-based stats. Identities = 14/50 (28%), Positives = 28/50 (56%) Query: 22 LNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVLAIYLRD 71 +N I + DP K+++ + H + Y ++ ++EW K + +G IY R+ Sbjct: 16 MNLAAIKKIDPYAKEIVDSSSHVAFYTFNSSQNEWEKTDVEGAFFIYHRN 65 >4CFF-A nrPDB Length = 571 Score = 34.3 bits (77), Expect = 0.041, Method: Composition-based stats. Identities = 23/133 (17%), Positives = 57/133 (42%), Gaps = 11/133 (8%) Query: 7 AAENSATQLEFYRKALNFNVIGRYDPKIKQLLF-----HTPHASLYKWDFKKDEWNKLEY 61 A + + L ++ ++++ +KQL F + H + + + + K+ Sbjct: 415 AVKKAKWHLGIRSQSKPYDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSL 474 Query: 62 QGVLAIYLRDVSQNTNLLPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKSN 121 Q +YL D + LL + ++ + ++GS+ Q + + +R S ++ + Sbjct: 475 Q----LYLVD--NRSYLLDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAES 528 Query: 122 DSLTYNCGKTLSG 134 SL+ + +L+G Sbjct: 529 HSLSGSLTGSLTG 541 >5EZV-A nrPDB Length = 560 Score = 33.6 bits (75), Expect = 0.076, Method: Composition-based stats. Identities = 21/116 (18%), Positives = 51/116 (43%), Gaps = 11/116 (9%) Query: 24 FNVIGRYDPKIKQLLF-----HTPHASLYKWDFKKDEWNKLEYQGVLAIYLRDVSQNTNL 78 ++++ +KQL F + H + + + + K+ Q +YL D + L Sbjct: 421 YDIMAEVYRAMKQLDFEWKVVNAYHLRVRRKNPVTGNYVKMSLQ----LYLVD--NRSYL 474 Query: 79 LPVSPQEVDIFDSQNGSNNIQVNNGSDNSNRNSSGNGNSYKSNDSLTYNCGKTLSG 134 L + ++ + ++GS+ Q + + +R S ++ + SL+ + +L+G Sbjct: 475 LDFKSIDDEVVEQRSGSSTPQRSCSAAGLHRPRSSFDSTTAESHSLSGSLTGSLTG 530 >4HZS-A nrPDB Length = 341 Score = 32.4 bits (72), Expect = 0.17, Method: Composition-based stats. Identities = 11/35 (31%), Positives = 16/35 (45%), Gaps = 4/35 (11%) Query: 198 HEVYGI----WIHTVSDRQNIYELIKYLLENEPKD 228 ++Y + W H DR L +LLE +P D Sbjct: 246 QDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTD 280 >1U54-B nrPDB Length = 291 Score = 32.4 bits (72), Expect = 0.17, Method: Composition-based stats. Identities = 11/35 (31%), Positives = 16/35 (45%), Gaps = 4/35 (11%) Query: 198 HEVYGI----WIHTVSDRQNIYELIKYLLENEPKD 228 ++Y + W H DR L +LLE +P D Sbjct: 252 QDIYNVMVQCWAHKPEDRPTFVALRDFLLEAQPTD 286 >4B6H-A nrPDB Length = 134 Score = 30.9 bits (68), Expect = 0.53, Method: Composition-based stats. Identities = 14/54 (25%), Positives = 28/54 (51%) Query: 20 KALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVLAIYLRDVS 73 + ++ + ++DP I + T +LY + K ++W K + +G L +Y R S Sbjct: 13 QEMSLAALKQHDPYITSIADLTGQVALYTFCPKANQWEKTDIEGTLFVYRRSAS 66 Score = 27.4 bits (59), Expect = 5.1, Method: Composition-based stats. Identities = 15/65 (23%), Positives = 32/65 (49%), Gaps = 3/65 (4%) Query: 161 SVVNKRKVFNAEEDTLNPLECMGVEVKDELVIIKNLKHEVYGIWIHTVSDRQNIYELIKY 220 ++VN+ + N E LE ++ + ++ +N +Y IW + +D I +L+ Sbjct: 72 TIVNRLNMHNLVEPVNKDLE---FQLHEPFLLYRNASLSIYSIWFYDKNDCHRIAKLMAD 128 Query: 221 LLENE 225 ++E E Sbjct: 129 VVEEE 133 >5KQ4-D nrPDB Length = 130 Score = 30.5 bits (67), Expect = 0.66, Method: Composition-based stats. Identities = 12/49 (24%), Positives = 25/49 (51%) Query: 17 FYRKALNFNVIGRYDPKIKQLLFHTPHASLYKWDFKKDEWNKLEYQGVL 65 R A+N V+ + P+I+ ++ H ++Y++D +W K +G Sbjct: 9 ILRNAVNLQVLKFHYPEIESIIDIASHVAVYQFDVGSQKWLKTSIEGTF 57 Score = 30.1 bits (66), Expect = 0.87, Method: Composition-based stats. Identities = 17/79 (21%), Positives = 30/79 (37%), Gaps = 21/79 (26%) Query: 141 GLIILNRINPDNFSMGIVPNSVVNKRKVFNAEEDTLNPLECMGVEVKDELVIIKNLKHEV 200 G +ILNR +P+N + I V + D +I + V Sbjct: 68 GYVILNRNSPENLYLFIN---------------------HPSNVHLVDRYLIHRTENQHV 106 Query: 201 YGIWIHTVSDRQNIYELIK 219 G+W+ +D I+ ++K Sbjct: 107 VGLWMFDPNDMSRIFNIVK 125 >1KZH-A nrPDB Length = 555 Score = 28.2 bits (61), Expect = 3.0, Method: Composition-based stats. Identities = 24/97 (24%), Positives = 46/97 (47%), Gaps = 12/97 (12%) Query: 143 IILNR-INPDNFSMGIVPNSVVN--------KRKVFNAEEDTLNPLECMGVEVKDELVII 193 +IL R +N DNF + IVP ++ ++ + + + + +E E+ + Sbjct: 294 VILKRSLNGDNFGVVIVPEGLIEFIPEVKSLMLELCDIFDKNEGEFKGLNIEKMKEIFVA 353 Query: 194 KNLKHEVYGIWIHTVSDRQNIYELIKYLLENEPKDSF 230 K L + G+++ Q +ELIK +LE +P +F Sbjct: 354 K-LSDYMKGVYLSLPLFIQ--FELIKSILERDPHGNF 387 >3O04-A nrPDB Length = 413 Score = 27.8 bits (60), Expect = 3.8, Method: Composition-based stats. Identities = 15/72 (20%), Positives = 31/72 (43%), Gaps = 7/72 (9%) Query: 141 GLIILNRINPDNFSMGIVP-------NSVVNKRKVFNAEEDTLNPLECMGVEVKDELVII 193 G+ + R+NPD+F + I + K++ + T + + V+D ++I Sbjct: 34 GVAKMTRLNPDDFPVKIAAELKDFDVEKYLEKKEARKMDRFTHYAIASAEMAVQDSGLVI 93 Query: 194 KNLKHEVYGIWI 205 + G+WI Sbjct: 94 DDSNANRVGVWI 105 >4N81-A nrPDB Length = 259 Score = 27.4 bits (59), Expect = 5.7, Method: Composition-based stats. Identities = 12/24 (50%), Positives = 14/24 (58%) Query: 122 DSLTYNCGKTLSGKDIYNYGLIIL 145 D + C TL G D YNYGL+ L Sbjct: 172 DKIRLKCRDTLLGGDCYNYGLLSL 195 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.128 0.364 Lambda K H 0.267 0.0366 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 8,548,322 Number of Sequences: 36641 Number of extensions: 336316 Number of successful extensions: 868 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 26 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 793 Number of HSP's gapped (non-prelim): 67 length of query: 231 length of database: 10,336,785 effective HSP length: 94 effective length of query: 137 effective length of database: 6,892,531 effective search space: 944276747 effective search space used: 944276747 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (22.0 bits) S2: 57 (26.7 bits)