BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q12308 (588 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 2J04-A nrPDB 1214 0.0 6BX3-B nrPDB 31 1.2 3GW8-A nrPDB 30 2.9 4NOI-A nrPDB 29 4.7 1VRM-A nrPDB 29 6.2 >2J04-A nrPDB Length = 588 Score = 1214 bits (3141), Expect = 0.0, Method: Compositional matrix adjust. Identities = 588/588 (100%), Positives = 588/588 (100%) Query: 1 MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKE 60 MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKE Sbjct: 1 MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKE 60 Query: 61 FPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTN 120 FPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTN Sbjct: 61 FPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTN 120 Query: 121 LDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAG 180 LDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAG Sbjct: 121 LDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAG 180 Query: 181 SKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVV 240 SKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVV Sbjct: 181 SKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVV 240 Query: 241 LTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHV 300 LTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHV Sbjct: 241 LTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHV 300 Query: 301 TADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKI 360 TADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKI Sbjct: 301 TADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKI 360 Query: 361 ASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPIS 420 ASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPIS Sbjct: 361 ASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPIS 420 Query: 421 LDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLS 480 LDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLS Sbjct: 421 LDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLS 480 Query: 481 IAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTT 540 IAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTT Sbjct: 481 IAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTT 540 Query: 541 HVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM 588 HVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM Sbjct: 541 HVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM 588 >6BX3-B nrPDB Length = 412 Score = 31.2 bits (69), Expect = 1.2, Method: Compositional matrix adjust. Identities = 30/128 (23%), Positives = 53/128 (41%), Gaps = 7/128 (5%) Query: 94 SPIDDWMAVLSNNGNVSVFKDNKMLTNLDSKGNLSS---RTYHCFEWNPIESSIVVGNED 150 SP D++A+ NG + ++ D + GN+ R W+P ++ + D Sbjct: 34 SPCGDYLALGCANGALVIY-DMDTFRPICVPGNMLGAHVRPITSIAWSPDGRLLLTSSRD 92 Query: 151 GELQFFSIRKNSENTPEFYFESSI---RLSDAGSKDWVTHIVWYEDVLVAALSNNSVFSM 207 ++ + + K S+ E F+S I + DA + V I D V SN+ V S+ Sbjct: 93 WSIKLWDLSKPSKPLKEIRFDSPIWGCQWLDAKRRLCVATIFEESDAYVIDFSNDPVASL 152 Query: 208 TVSASSHQ 215 + Q Sbjct: 153 LSKSDEKQ 160 >3GW8-A nrPDB Length = 257 Score = 30.0 bits (66), Expect = 2.9, Method: Compositional matrix adjust. Identities = 20/77 (25%), Positives = 34/77 (44%), Gaps = 4/77 (5%) Query: 311 EKKFKKWSTI-WNEFNNYETTLVIHGISLSPDGYSIAIVY-DMERVAFKYKIASEQSFNI 368 E +F W + E N E G L GY+ I Y + + A + + ++ Sbjct: 25 ENRFTGWVDVDLTEQGNREARQA--GQLLKEAGYTFDIAYTSVLKRAIRTLWHVQDQMDL 82 Query: 369 MFAPLYHTWTISERAVG 385 M+ P+ H+W ++ER G Sbjct: 83 MYVPVVHSWRLNERHYG 99 >4NOI-A nrPDB Length = 361 Score = 29.3 bits (64), Expect = 4.7, Method: Compositional matrix adjust. Identities = 16/67 (23%), Positives = 33/67 (49%), Gaps = 1/67 (1%) Query: 70 DFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTNLDSKGNLSS 129 D ++ N+ Y ++ I + +V+ N + ++ K+L N+ + NLS+ Sbjct: 228 DGQITPNEAFQNALEAMYKQLSVFDKITNVRSVIKNQATSNELENTKLLQNI-TDLNLSA 286 Query: 130 RTYHCFE 136 R+Y+C E Sbjct: 287 RSYNCLE 293 >1VRM-A nrPDB Length = 325 Score = 28.9 bits (63), Expect = 6.2, Method: Compositional matrix adjust. Identities = 33/158 (20%), Positives = 65/158 (41%), Gaps = 21/158 (13%) Query: 331 LVIHGISLSPDGYSIAIVYDMERVAFKYKIASEQSF--------NIMFAPLYHTWTISER 382 +V+ ++P + AI+ DM+R+ +K+ E+S N T+++ + Sbjct: 32 IVVSSQKINPRTIAEAILEDMKRITYKFSFTDERSVVKKINDHPNEWVEVDEETYSLIKA 91 Query: 383 AVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPI------SLDFQSYLNALMKSEEM 436 A A T ++ ++ +L E + N P +L Y N L + M Sbjct: 92 ACAFAEL-TDGAFDPTVGRLLELWGFTGNYENLRVPSREEIEEALKHTGYKNVLFDDKNM 150 Query: 437 RIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFD 474 R+++ + ID I YA+++ ++ SFD Sbjct: 151 RVMVKNGVKIDLGGIAK------GYALDRARQIALSFD 182 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 2J04-A nrPDB 1258 0.0 Sequences not found previously or not previously below threshold: 6BX3-B nrPDB 33 0.50 3GW8-A nrPDB 30 2.5 4PXG-A nrPDB 30 2.7 4NOI-A nrPDB 30 3.2 4KQB-A nrPDB 30 4.3 1OJM-A nrPDB 29 5.0 5ZI5-A nrPDB 29 7.4 5WLC-LL nrPDB 28 9.2 CONVERGED! >2J04-A nrPDB Length = 588 Score = 1258 bits (3254), Expect = 0.0, Method: Composition-based stats. Identities = 588/588 (100%), Positives = 588/588 (100%) Query: 1 MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKE 60 MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKE Sbjct: 1 MKLLKDLLVDRKEFEDWKNNLTWARDGTLYLTTFPDISIGQPKYAKDINCNSKNLFHVKE 60 Query: 61 FPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTN 120 FPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTN Sbjct: 61 FPLEFENKLDFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTN 120 Query: 121 LDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAG 180 LDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAG Sbjct: 121 LDSKGNLSSRTYHCFEWNPIESSIVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSDAG 180 Query: 181 SKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVV 240 SKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVV Sbjct: 181 SKDWVTHIVWYEDVLVAALSNNSVFSMTVSASSHQPVSRMIQNASRRKITDLKIVDYKVV 240 Query: 241 LTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHV 300 LTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHV Sbjct: 241 LTCPGYVHKIDLKNYSISSLKTGSLENFHIIPLNHEKESTILLMSNKTSYKVLLEDELHV 300 Query: 301 TADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKI 360 TADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKI Sbjct: 301 TADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGISLSPDGYSIAIVYDMERVAFKYKI 360 Query: 361 ASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPIS 420 ASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPIS Sbjct: 361 ASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQSLPKLPENFSMNKKLLNGNYPIS 420 Query: 421 LDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLS 480 LDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLS Sbjct: 421 LDFQSYLNALMKSEEMRIIMFLNMTIDKPSILSFLEALYEYAINKKSELTNSFDLACVLS 480 Query: 481 IAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTT 540 IAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTT Sbjct: 481 IAAILKREAPIYNGTLLMKNSFLEETFNLESFTADPETVTSTTNNTWKRCGVTLLPILTT 540 Query: 541 HVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM 588 HVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM Sbjct: 541 HVKICPVSKQRVIDIKRDDLNDYGWFTRGLLERFNEISVYCGTTLEVM 588 >6BX3-B nrPDB Length = 412 Score = 32.7 bits (73), Expect = 0.50, Method: Composition-based stats. Identities = 30/128 (23%), Positives = 53/128 (41%), Gaps = 7/128 (5%) Query: 94 SPIDDWMAVLSNNGNVSVFKDNKMLTNLDSKGNL---SSRTYHCFEWNPIESSIVVGNED 150 SP D++A+ NG + ++ D + GN+ R W+P ++ + D Sbjct: 34 SPCGDYLALGCANGALVIY-DMDTFRPICVPGNMLGAHVRPITSIAWSPDGRLLLTSSRD 92 Query: 151 GELQFFSIRKNSENTPEFYFESSI---RLSDAGSKDWVTHIVWYEDVLVAALSNNSVFSM 207 ++ + + K S+ E F+S I + DA + V I D V SN+ V S+ Sbjct: 93 WSIKLWDLSKPSKPLKEIRFDSPIWGCQWLDAKRRLCVATIFEESDAYVIDFSNDPVASL 152 Query: 208 TVSASSHQ 215 + Q Sbjct: 153 LSKSDEKQ 160 >3GW8-A nrPDB Length = 257 Score = 30.4 bits (67), Expect = 2.5, Method: Composition-based stats. Identities = 20/77 (25%), Positives = 34/77 (44%), Gaps = 4/77 (5%) Query: 311 EKKFKKWSTI-WNEFNNYETTLVIHGISLSPDGYSIAIVY-DMERVAFKYKIASEQSFNI 368 E +F W + E N E G L GY+ I Y + + A + + ++ Sbjct: 25 ENRFTGWVDVDLTEQGNREARQA--GQLLKEAGYTFDIAYTSVLKRAIRTLWHVQDQMDL 82 Query: 369 MFAPLYHTWTISERAVG 385 M+ P+ H+W ++ER G Sbjct: 83 MYVPVVHSWRLNERHYG 99 >4PXG-A nrPDB Length = 509 Score = 30.0 bits (66), Expect = 2.7, Method: Composition-based stats. Identities = 22/71 (30%), Positives = 38/71 (53%), Gaps = 5/71 (7%) Query: 351 MERVAFKYKIASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIY-NQSLPKLPENFSMN 409 M + KY + E+ +I+ P + I E+++ ++ +Q Y N S+ KL E+FS+N Sbjct: 228 MTSILRKYVLGDEKIDSIVKDP----FEIKEKSIEDIVFEKFQPYINWSIDKLCEHFSIN 283 Query: 410 KKLLNGNYPIS 420 K NY I+ Sbjct: 284 KGEKGLNYRIA 294 >4NOI-A nrPDB Length = 361 Score = 30.0 bits (66), Expect = 3.2, Method: Composition-based stats. Identities = 16/67 (23%), Positives = 33/67 (49%), Gaps = 1/67 (1%) Query: 70 DFELAQQNGLLNSQPVCYPRVCKPSPIDDWMAVLSNNGNVSVFKDNKMLTNLDSKGNLSS 129 D ++ N+ Y ++ I + +V+ N + ++ K+L N+ + NLS+ Sbjct: 228 DGQITPNEAFQNALEAMYKQLSVFDKITNVRSVIKNQATSNELENTKLLQNI-TDLNLSA 286 Query: 130 RTYHCFE 136 R+Y+C E Sbjct: 287 RSYNCLE 293 >4KQB-A nrPDB Length = 453 Score = 29.7 bits (65), Expect = 4.3, Method: Composition-based stats. Identities = 22/78 (28%), Positives = 36/78 (46%), Gaps = 4/78 (5%) Query: 97 DDWMAVLSNNGNVSVFKDNKMLTNLDSKGNLSS-RTYHCFEWNPIESSIVVGNEDGELQF 155 D+W SN G+ SV+ D++M+ NL N S + H + + D + F Sbjct: 73 DNWERFYSNIGSCSVYSDDQMIDNLLHDLNTSPIKHVHIMDGGTQVKFVFTFKNDKQAVF 132 Query: 156 FSIR--KNSENTP-EFYF 170 +R ++ E+ P FYF Sbjct: 133 KPMRFGRDYESDPNHFYF 150 >1OJM-A nrPDB Length = 729 Score = 29.3 bits (64), Expect = 5.0, Method: Composition-based stats. Identities = 19/49 (38%), Positives = 24/49 (48%), Gaps = 6/49 (12%) Query: 493 NGTL-LMKNSFLEETFN-----LESFTADPETVTSTTNNTWKRCGVTLL 535 N TL LMK F +E +E F DPE TT+N +K G L+ Sbjct: 134 NNTLSLMKEYFSDEEIKKYTDVIEKFVPDPEHFRKTTDNPFKALGGNLV 182 >5ZI5-A nrPDB Length = 921 Score = 28.9 bits (63), Expect = 7.4, Method: Composition-based stats. Identities = 77/322 (23%), Positives = 123/322 (38%), Gaps = 68/322 (21%) Query: 227 RKITDLKIVDYKVVLTCPGYVHKIDLKNYSISSLKTGSLENFHIIP-LNHEKESTIL--- 282 +K++D K +DY +V +DL+ +S S ++P LN + S L Sbjct: 37 KKLSDYKSLDY--------FVIHVDLQ-IDLSKKPVESKARLTVVPNLNVDSHSNDLVLD 87 Query: 283 -----LMSNKTSYKVLLEDELHVTADNIIAPYLEKKFKKWSTIWNEFNNYETTLVIHGIS 337 L+S + + +L E+E +T D++I + + T I S Sbjct: 88 GENMTLVSLQMNDNLLKENEYELTKDSLIIKNIPQN---------------TPFTIEMTS 132 Query: 338 LSPDGYSIAIVYDMERVAFKYKIASEQSFNIMFAPLYHTWTISERAVGLAWYQTYQIYNQ 397 L + + +Y+ E VA K SE + + P +R LA Y+T I NQ Sbjct: 133 LLGENTDLFGLYETEGVAL-VKAESEGLRRVFYLP--------DRPDNLATYKTTIIANQ 183 Query: 398 S-LPKLPENFSM--NKKLLNGNYPIS-LD---FQSYLNALMKSEEMRIIMFLNMTIDK-- 448 P L N + K+L G + ++ LD SYL AL+ R + + + Sbjct: 184 EDYPVLLSNGVLIEKKELPLGLHSVTWLDDVPKPSYLFALVAGNLQRSVTYYQTKSGREL 243 Query: 449 -------PSILSFLEALYEYAINKKSELTNSFDLACVLS---IAAILKREAPIYNGTLLM 498 PS S + E + +F+L C L +A + K + T L Sbjct: 244 PIEFYVPPSATSKCDFAKEVLKEAMAWDERTFNLECALRQHMVAGVDKYASGASEPTGL- 302 Query: 499 KNSFLEETFNLESFTADPETVT 520 FN E+ A PET T Sbjct: 303 ------NLFNTENLFASPETKT 318 >5WLC-LL nrPDB Length = 643 Score = 28.5 bits (62), Expect = 9.2, Method: Composition-based stats. Identities = 35/134 (26%), Positives = 64/134 (47%), Gaps = 21/134 (15%) Query: 134 CFEWNPIESS----IVVGNEDGELQFFSIRKNSENTPEFYFESSIRLSD---AGSKDWV- 185 C +W + SS I++G +GE+ +S+ N E T +F +S + D G++ W Sbjct: 62 CLKWVNLASSDTVAIILGMNNGEIWLYSVLAN-EVTYKFTTGNSYEIKDIDLMGNQLWCI 120 Query: 186 -THIVWYE-DVLVAAL-----SNNSV--FSMTVSASSHQPVSRMIQNASRRKITDLKIVD 236 + +Y+ D+L L NN V +T+ + ++ + S I+ + I + Sbjct: 121 DSSDAFYQFDLLQFKLLQHFRINNCVQLNKLTIVPAGDSVAQLLVASHS---ISLIDIEE 177 Query: 237 YKVVLTCPGYVHKI 250 KVV+T PG+V + Sbjct: 178 KKVVMTFPGHVSPV 191 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.318 0.134 0.398 Lambda K H 0.267 0.0432 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 26,918,687 Number of Sequences: 36641 Number of extensions: 1250194 Number of successful extensions: 2792 Number of sequences better than 10.0: 11 Number of HSP's better than 10.0 without gapping: 3 Number of HSP's successfully gapped in prelim test: 18 Number of HSP's that attempted gapping in prelim test: 2784 Number of HSP's gapped (non-prelim): 28 length of query: 588 length of database: 10,336,785 effective HSP length: 102 effective length of query: 486 effective length of database: 6,599,403 effective search space: 3207309858 effective search space used: 3207309858 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 62 (28.4 bits)