BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q10477 (273 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5U0S-2 nrPDB 557 e-159 2N6L-A nrPDB 33 0.17 3LB6-C nrPDB 28 3.0 6I44-A nrPDB 28 3.9 1WA5-C nrPDB 28 3.9 1H9G-A nrPDB 28 6.1 1YNF-A nrPDB 27 9.1 2XS0-A nrPDB 27 9.4 >5U0S-2 nrPDB Length = 273 Score = 557 bits (1436), Expect = e-159, Method: Compositional matrix adjust. Identities = 273/273 (100%), Positives = 273/273 (100%) Query: 1 MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEK 60 MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEK Sbjct: 1 MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEK 60 Query: 61 VNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDL 120 VNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDL Sbjct: 61 VNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDL 120 Query: 121 KEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTR 180 KEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTR Sbjct: 121 KEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTR 180 Query: 181 GKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLE 240 GKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLE Sbjct: 181 GKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLE 240 Query: 241 LPSVRRNANSTVKPTSKDILALHAECVPAQSDL 273 LPSVRRNANSTVKPTSKDILALHAECVPAQSDL Sbjct: 241 LPSVRRNANSTVKPTSKDILALHAECVPAQSDL 273 >2N6L-A nrPDB Length = 215 Score = 32.7 bits (73), Expect = 0.17, Method: Compositional matrix adjust. Identities = 29/107 (27%), Positives = 47/107 (43%), Gaps = 9/107 (8%) Query: 22 LHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEKVNSFYTHLKSSQNVFDYAEKS 81 L +LA+++ + P+LL +P GT+ F+ + F L S++ ++ Sbjct: 80 LDGKLADIKQLPPTLLLQYYPMGGTNSAFQPYGGLGVNYTTFFDEDLASNRKAQGFSSMK 139 Query: 82 LQADSSNLLP----TYLYNSEDLSNDT----ENNETKSINGKSALDL 120 LQ DS L Y+ N L N + + SING SAL + Sbjct: 140 LQ-DSWGLAGELGFDYMLNEHALFNMAVWYMDIDTKASINGPSALGV 185 >3LB6-C nrPDB Length = 380 Score = 28.5 bits (62), Expect = 3.0, Method: Compositional matrix adjust. Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 4/42 (9%) Query: 154 ILTTHENKSFTLKLANELEFIFFHDTRGKFSVYCSSSKDDAI 195 + T EN+++TLK NE + F R K ++YCS DD I Sbjct: 284 VTATVENETYTLKTTNETRQLCFV-VRSKVNIYCS---DDGI 321 >6I44-A nrPDB Length = 627 Score = 28.1 bits (61), Expect = 3.9, Method: Compositional matrix adjust. Identities = 16/57 (28%), Positives = 28/57 (49%), Gaps = 4/57 (7%) Query: 94 LYNSEDLSNDTENNETKSINGKSALDLK----EPHHSELHDNDNFQNSDINIESFKG 146 L SE + + + +I+G S L K EP HS+++ +F ++N+ KG Sbjct: 247 LKTSESGTPSSSTPQENTISGYSLLTCKRTLPEPCHSKIYPGVDFGGEELNVTFVKG 303 >1WA5-C nrPDB Length = 960 Score = 28.1 bits (61), Expect = 3.9, Method: Compositional matrix adjust. Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 5/55 (9%) Query: 56 EAIEKVNSFYTHLKSSQNV-FDYA----EKSLQADSSNLLPTYLYNSEDLSNDTE 105 + IE + T L++ + V + YA EK L SN P ++++ ED+SN TE Sbjct: 493 QLIELMPILATFLQTDEYVVYTYAAITIEKILTIRESNTSPAFIFHKEDISNSTE 547 >1H9G-A nrPDB Length = 243 Score = 27.7 bits (60), Expect = 6.1, Method: Compositional matrix adjust. Identities = 26/96 (27%), Positives = 34/96 (35%), Gaps = 18/96 (18%) Query: 132 DNFQNSDINIESFKGDIEASG------------SILTTHENKSFTLKLANELEFIFFHDT 179 D F D NI F+G ASG + T F A L F+H Sbjct: 143 DAFAELDYNI--FRGLAFASGNPIYGLILNGMKGLYTRIGRHYFANPEARSLALGFYH-- 198 Query: 180 RGKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPK 215 K S CS D + ++ R + G +W M K Sbjct: 199 --KLSALCSEGAHDQVYETVRRYGHESGEIWHRMQK 232 >1YNF-A nrPDB Length = 458 Score = 26.9 bits (58), Expect = 9.1, Method: Compositional matrix adjust. Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 1/50 (2%) Query: 55 QEAIEKVNSFYTHLKSSQNVFDYAE-KSLQADSSNLLPTYLYNSEDLSND 103 Q+A + + +L++ N F E + Q S+ + TYL+NS+ LS D Sbjct: 275 QQAFARQSQLLANLRARVNGFMAIEVPATQVSVSDTVSTYLFNSQLLSRD 324 >2XS0-A nrPDB Length = 386 Score = 26.9 bits (58), Expect = 9.4, Method: Compositional matrix adjust. Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 10/78 (12%) Query: 19 LNQLHEQLAELRTICPSLLKLLHPETGT---------SRKFEKSAQEAIEKVNSFYTHLK 69 ++Q ++ + +L T CP +K L P T FEK + + +S + LK Sbjct: 233 IDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLK 292 Query: 70 SSQNVFDYAEKSLQADSS 87 +SQ D K L D+S Sbjct: 293 ASQ-ARDLLSKMLVIDAS 309 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5U0S-2 nrPDB 549 e-157 Sequences not found previously or not previously below threshold: 2N6L-A nrPDB 32 0.33 1WA5-C nrPDB 31 0.51 3LB6-C nrPDB 31 0.61 4YY0-B nrPDB 30 1.0 4WSV-B nrPDB 30 1.0 1KHO-A nrPDB 30 1.4 5YFP-B nrPDB 30 1.6 2D4H-A nrPDB 29 1.9 1F5N-A nrPDB 29 1.9 5GY2-A nrPDB 29 2.0 2XS0-A nrPDB 29 2.0 1YNF-A nrPDB 29 3.3 5BNY-B nrPDB 28 5.6 CONVERGED! >5U0S-2 nrPDB Length = 273 Score = 549 bits (1414), Expect = e-157, Method: Composition-based stats. Identities = 273/273 (100%), Positives = 273/273 (100%) Query: 1 MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEK 60 MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEK Sbjct: 1 MSLEEQRTRDELKHKLLDLNQLHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEK 60 Query: 61 VNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDL 120 VNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDL Sbjct: 61 VNSFYTHLKSSQNVFDYAEKSLQADSSNLLPTYLYNSEDLSNDTENNETKSINGKSALDL 120 Query: 121 KEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTR 180 KEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTR Sbjct: 121 KEPHHSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTR 180 Query: 181 GKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLE 240 GKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLE Sbjct: 181 GKFSVYCSSSKDDAITFSINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPVYLE 240 Query: 241 LPSVRRNANSTVKPTSKDILALHAECVPAQSDL 273 LPSVRRNANSTVKPTSKDILALHAECVPAQSDL Sbjct: 241 LPSVRRNANSTVKPTSKDILALHAECVPAQSDL 273 >2N6L-A nrPDB Length = 215 Score = 31.6 bits (70), Expect = 0.33, Method: Composition-based stats. Identities = 27/108 (25%), Positives = 47/108 (43%), Gaps = 7/108 (6%) Query: 22 LHEQLAELRTICPSLLKLLHPETGTSRKFEKSAQEAIEKVNSFYTHLKSSQNVFDYAEKS 81 L +LA+++ + P+LL +P GT+ F+ + F L S++ ++ Sbjct: 80 LDGKLADIKQLPPTLLLQYYPMGGTNSAFQPYGGLGVNYTTFFDEDLASNRKAQGFSSMK 139 Query: 82 LQAD---SSNLLPTYLYNSEDLSNDT----ENNETKSINGKSALDLKE 122 LQ + L Y+ N L N + + SING SAL + + Sbjct: 140 LQDSWGLAGELGFDYMLNEHALFNMAVWYMDIDTKASINGPSALGVNK 187 >1WA5-C nrPDB Length = 960 Score = 31.2 bits (69), Expect = 0.51, Method: Composition-based stats. Identities = 19/55 (34%), Positives = 30/55 (54%), Gaps = 5/55 (9%) Query: 56 EAIEKVNSFYTHLKSSQNV-FDYA----EKSLQADSSNLLPTYLYNSEDLSNDTE 105 + IE + T L++ + V + YA EK L SN P ++++ ED+SN TE Sbjct: 493 QLIELMPILATFLQTDEYVVYTYAAITIEKILTIRESNTSPAFIFHKEDISNSTE 547 >3LB6-C nrPDB Length = 380 Score = 30.9 bits (68), Expect = 0.61, Method: Composition-based stats. Identities = 17/42 (40%), Positives = 24/42 (57%), Gaps = 4/42 (9%) Query: 154 ILTTHENKSFTLKLANELEFIFFHDTRGKFSVYCSSSKDDAI 195 + T EN+++TLK NE + F R K ++YCS DD I Sbjct: 284 VTATVENETYTLKTTNETRQLCFV-VRSKVNIYCS---DDGI 321 >4YY0-B nrPDB Length = 159 Score = 30.1 bits (66), Expect = 1.0, Method: Composition-based stats. Identities = 27/94 (28%), Positives = 42/94 (44%), Gaps = 13/94 (13%) Query: 52 KSAQEAIE----KVNSFYTHLKSSQNVFDYAEKSLQADSSNL--------LPTYLYNSED 99 +S Q+AI+ KVNS + + D+ +L+ NL L + YN+E Sbjct: 39 ESTQKAIDGITNKVNSIINKMNTQFEAVDHEFSNLERRIGNLNKRMEDGFLDVWTYNAEL 98 Query: 100 LSNDTENNETKSINGKSALDLKEPHHSELHDNDN 133 L EN T ++ + +L E S+L DN N Sbjct: 99 LVL-LENERTLDLHDANVKNLYEKVKSQLRDNAN 131 >4WSV-B nrPDB Length = 181 Score = 30.1 bits (66), Expect = 1.0, Method: Composition-based stats. Identities = 27/94 (28%), Positives = 42/94 (44%), Gaps = 13/94 (13%) Query: 52 KSAQEAIE----KVNSFYTHLKSSQNVFDYAEKSLQADSSNL--------LPTYLYNSED 99 +S Q+AI+ KVNS + + D+ +L+ NL L + YN+E Sbjct: 39 ESTQKAIDGITNKVNSIINKMNTQFEAVDHEFSNLERRIGNLNKRMEDGFLDVWTYNAEL 98 Query: 100 LSNDTENNETKSINGKSALDLKEPHHSELHDNDN 133 L EN T ++ + +L E S+L DN N Sbjct: 99 LVL-LENERTLDLHDANVKNLYEKVKSQLRDNAN 131 >1KHO-A nrPDB Length = 370 Score = 29.7 bits (65), Expect = 1.4, Method: Composition-based stats. Identities = 19/70 (27%), Positives = 37/70 (52%), Gaps = 9/70 (12%) Query: 47 SRKFEKSAQEAIEKVNSFYTH--LKSSQNVFDYAEKSLQADSSNLLPTYLYNS-EDLSND 103 +R F K+A++ +Y+H + S + +DYA K A+S Y+Y D+S+ Sbjct: 193 ARSFAKTAKDL------YYSHANMSCSWDEWDYAAKVALANSQKGTSGYIYRFLHDVSDG 246 Query: 104 TENNETKSIN 113 +++ K++N Sbjct: 247 KDSSANKNVN 256 >5YFP-B nrPDB Length = 971 Score = 29.7 bits (65), Expect = 1.6, Method: Composition-based stats. Identities = 29/106 (27%), Positives = 49/106 (46%), Gaps = 12/106 (11%) Query: 127 ELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFT--LKLANELEFIFFH-DTRGKF 183 ++H D+F + + +++ DI+ L K+FT +K+ N+L+ I+ D + Sbjct: 119 DMHKQDSFNDLNNSLDRLDSDIQDQSIHLKQLVGKNFTKYVKIKNKLDQIYKEFDEKTNE 178 Query: 184 SVYCSSSKDDAITF-SIN-------RNNNF-LGNLWSLMPKILDYQ 220 C S K++ I S+N R F L L KIL+YQ Sbjct: 179 KNQCDSPKENQINVESLNKKVDEVIRTTTFKLKPLMDNYQKILNYQ 224 >2D4H-A nrPDB Length = 328 Score = 29.3 bits (64), Expect = 1.9, Method: Composition-based stats. Identities = 27/90 (30%), Positives = 42/90 (46%), Gaps = 14/90 (15%) Query: 89 LLPTYLYNSEDLSNDTENNE-------TKSINGKSALDLKEPHHSELHDNDNFQN--SDI 139 L T++YNS N ++ T I KS+ D E +E+ D+ +F + D Sbjct: 133 LSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENE---NEVEDSADFVSFFPDF 189 Query: 140 --NIESFKGDIEASGSILTTHENKSFTLKL 167 + F D+EA G LT E +++LKL Sbjct: 190 VWTLRDFSLDLEADGQPLTPDEYLTYSLKL 219 >1F5N-A nrPDB Length = 592 Score = 29.3 bits (64), Expect = 1.9, Method: Composition-based stats. Identities = 27/90 (30%), Positives = 42/90 (46%), Gaps = 14/90 (15%) Query: 89 LLPTYLYNSEDLSNDTENNE-------TKSINGKSALDLKEPHHSELHDNDNFQN--SDI 139 L T++YNS N ++ T I KS+ D E +E+ D+ +F + D Sbjct: 122 LSSTFVYNSIGTINQQAMDQLYYVTELTHRIRSKSSPDENE---NEVEDSADFVSFFPDF 178 Query: 140 --NIESFKGDIEASGSILTTHENKSFTLKL 167 + F D+EA G LT E +++LKL Sbjct: 179 VWTLRDFSLDLEADGQPLTPDEYLTYSLKL 208 >5GY2-A nrPDB Length = 455 Score = 29.3 bits (64), Expect = 2.0, Method: Composition-based stats. Identities = 37/134 (27%), Positives = 57/134 (42%), Gaps = 18/134 (13%) Query: 125 HSELHDNDNFQNSDINIESFKGDIEASGSILTTHENKSFTLKLANELEFIFFHDTRGKFS 184 H+ D DNF +FKG +EASG + T +KS L + F F D Sbjct: 258 HTNFKDPDNF--------TFKG-LEASG-VKTCDLSKSKIFALLKSV-FSHFTDLEQLTL 306 Query: 185 VYCSSSKDDAITF-------SINRNNNFLGNLWSLMPKILDYQHLYSKPCDFCKSLISPV 237 +K D F +N + NFLG++ S M + LD + + ++L Sbjct: 307 AQNEINKIDDNAFWGLTHLLKLNLSQNFLGSIDSRMFENLDKLEVLDLSYNHIRALGDQS 366 Query: 238 YLELPSVRRNANST 251 +L LP+++ A T Sbjct: 367 FLGLPNLKELALDT 380 >2XS0-A nrPDB Length = 386 Score = 29.3 bits (64), Expect = 2.0, Method: Composition-based stats. Identities = 22/78 (28%), Positives = 35/78 (44%), Gaps = 10/78 (12%) Query: 19 LNQLHEQLAELRTICPSLLKLLHPETGT---------SRKFEKSAQEAIEKVNSFYTHLK 69 ++Q ++ + +L T CP +K L P T FEK + + +S + LK Sbjct: 233 IDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLK 292 Query: 70 SSQNVFDYAEKSLQADSS 87 +SQ D K L D+S Sbjct: 293 ASQ-ARDLLSKMLVIDAS 309 >1YNF-A nrPDB Length = 458 Score = 28.5 bits (62), Expect = 3.3, Method: Composition-based stats. Identities = 16/50 (32%), Positives = 27/50 (54%), Gaps = 1/50 (2%) Query: 55 QEAIEKVNSFYTHLKSSQNVFDYAE-KSLQADSSNLLPTYLYNSEDLSND 103 Q+A + + +L++ N F E + Q S+ + TYL+NS+ LS D Sbjct: 275 QQAFARQSQLLANLRARVNGFMAIEVPATQVSVSDTVSTYLFNSQLLSRD 324 >5BNY-B nrPDB Length = 191 Score = 27.8 bits (60), Expect = 5.6, Method: Composition-based stats. Identities = 26/95 (27%), Positives = 42/95 (44%), Gaps = 13/95 (13%) Query: 51 EKSAQEAIE----KVNSFYTHLKSSQNVFDYAEKSLQADSSNL--------LPTYLYNSE 98 ++S Q+AI+ KVNS + + + +L+ NL L + YN+E Sbjct: 38 KESTQKAIDGITNKVNSIIDKMNTQFEAVGHEFSNLERRIDNLNKRMEDGFLDVWTYNAE 97 Query: 99 DLSNDTENNETKSINGKSALDLKEPHHSELHDNDN 133 L EN T ++ + +L E S+L DN N Sbjct: 98 LLVL-LENERTLDLHDANVKNLHEKVRSQLRDNAN 131 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.313 0.129 0.365 Lambda K H 0.267 0.0400 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,582,060 Number of Sequences: 36641 Number of extensions: 417196 Number of successful extensions: 883 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 5 Number of HSP's successfully gapped in prelim test: 35 Number of HSP's that attempted gapping in prelim test: 878 Number of HSP's gapped (non-prelim): 40 length of query: 273 length of database: 10,336,785 effective HSP length: 96 effective length of query: 177 effective length of database: 6,819,249 effective search space: 1207007073 effective search space used: 1207007073 T: 11 A: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 58 (27.0 bits)