BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q10469 (447 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5VCM-A nrPDB 880 0.0 5NA2-A nrPDB 31 0.99 3LHX-A nrPDB 30 2.2 5GUW-B nrPDB 29 4.9 5LLW-B nrPDB 28 6.1 6GRI-C nrPDB 28 8.3 >5VCM-A nrPDB Length = 419 Score = 880 bits (2273), Expect = 0.0, Method: Compositional matrix adjust. Identities = 419/419 (100%), Positives = 419/419 (100%) Query: 29 GRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLT 88 GRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLT Sbjct: 1 GRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLT 60 Query: 89 LRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVL 148 LRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVL Sbjct: 61 LRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVL 120 Query: 149 VIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKL 208 VIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKL Sbjct: 121 VIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKL 180 Query: 209 GCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDF 268 GCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDF Sbjct: 181 GCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDF 240 Query: 269 YHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAY 328 YHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAY Sbjct: 241 YHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAY 300 Query: 329 QKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRP 388 QKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRP Sbjct: 301 QKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRP 360 Query: 389 STQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ 447 STQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ Sbjct: 361 STQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ 419 >5NA2-A nrPDB Length = 213 Score = 31.2 bits (69), Expect = 0.99, Method: Compositional matrix adjust. Identities = 18/77 (23%), Positives = 33/77 (42%), Gaps = 3/77 (3%) Query: 128 PEYLRLLLDSLRKAQGIDNV---LVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLY 184 P+ + + ++ R+ G + V FS + T++ LI C + S++ Sbjct: 16 PKMVSIFMEKAREGLGGEEVQLWFTAFSANLTPTDMATLIMAAPGCAADKEILDESLKQL 75 Query: 185 PNEFPGSDPRDCPRDLP 201 E+ + P D PR LP Sbjct: 76 TAEYDRTHPPDAPRPLP 92 >3LHX-A nrPDB Length = 319 Score = 30.0 bits (66), Expect = 2.2, Method: Compositional matrix adjust. Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 7/43 (16%) Query: 310 KTWKSTEHNMGLALTRNAYQKLIECTD-TFCTYDDYNWDWTLQ 351 + W S E T+ YQ+++ECTD F T DD + W Q Sbjct: 174 RLWASKEE------TQQVYQQMLECTDIAFLTLDDEDALWGQQ 210 >5GUW-B nrPDB Length = 465 Score = 28.9 bits (63), Expect = 4.9, Method: Compositional matrix adjust. Identities = 14/52 (26%), Positives = 20/52 (38%), Gaps = 4/52 (7%) Query: 198 RDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHF----VWERV 245 R + L G I F Y ++ K +WWW +H VWE + Sbjct: 166 RKTAISMVLMTGLIGLALLFLFSFYNPENLTRDKFYWWWVVHLWVEGVWELI 217 >5LLW-B nrPDB Length = 685 Score = 28.5 bits (62), Expect = 6.1, Method: Compositional matrix adjust. Identities = 11/32 (34%), Positives = 18/32 (56%) Query: 102 QTLRNVDKAGTWAPRELVLVVQVHNRPEYLRL 133 +TLR+ DK G W E +++ PE ++L Sbjct: 595 ETLRDTDKFGRWGGEEFLIIAPQTGMPELMQL 626 >6GRI-C nrPDB Length = 272 Score = 28.1 bits (61), Expect = 8.3, Method: Compositional matrix adjust. Identities = 20/64 (31%), Positives = 29/64 (45%), Gaps = 2/64 (3%) Query: 143 GIDNVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPK 202 G +V+ I S +S E +L +G+NFC ++ +L N F DP R P Sbjct: 65 GFSSVINISSSHNFSRE--RLPSGINFCDKNKLSIRTIEKLLVNAFSSPDPGSVRRPYPS 122 Query: 203 NAAL 206 AL Sbjct: 123 GGAL 126 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5VCM-A nrPDB 925 0.0 Sequences not found previously or not previously below threshold: 3LHX-A nrPDB 30 1.7 5LLW-B nrPDB 29 4.6 5GUW-B nrPDB 29 4.8 5GGG-A nrPDB 28 5.8 CONVERGED! >5VCM-A nrPDB Length = 419 Score = 925 bits (2391), Expect = 0.0, Method: Composition-based stats. Identities = 419/419 (100%), Positives = 419/419 (100%) Query: 29 GRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLT 88 GRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLT Sbjct: 1 GRQRKNEALAPPLLDAEPARGAGGRGGDHPSVAVGIRRVSNVSAASLVPAVPQPEADNLT 60 Query: 89 LRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVL 148 LRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVL Sbjct: 61 LRYRSLVYQLNFDQTLRNVDKAGTWAPRELVLVVQVHNRPEYLRLLLDSLRKAQGIDNVL 120 Query: 149 VIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKL 208 VIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKL Sbjct: 121 VIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLYPNEFPGSDPRDCPRDLPKNAALKL 180 Query: 209 GCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDF 268 GCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDF Sbjct: 181 GCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWERVKILRDYAGLILFLEEDHYLAPDF 240 Query: 269 YHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAY 328 YHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAY Sbjct: 241 YHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMADKVDVKTWKSTEHNMGLALTRNAY 300 Query: 329 QKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRP 388 QKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRP Sbjct: 301 QKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKVLVPQIPRIFHAGDCGMHHKKTCRP 360 Query: 389 STQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ 447 STQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ Sbjct: 361 STQSAQIESLLNNNKQYMFPETLTISEKFTVVAISPPRKNGGWGDIRDHELCKSYRRLQ 419 >3LHX-A nrPDB Length = 319 Score = 30.4 bits (67), Expect = 1.7, Method: Composition-based stats. Identities = 16/43 (37%), Positives = 22/43 (51%), Gaps = 7/43 (16%) Query: 310 KTWKSTEHNMGLALTRNAYQKLIECTD-TFCTYDDYNWDWTLQ 351 + W S E T+ YQ+++ECTD F T DD + W Q Sbjct: 174 RLWASKEE------TQQVYQQMLECTDIAFLTLDDEDALWGQQ 210 >5LLW-B nrPDB Length = 685 Score = 28.8 bits (63), Expect = 4.6, Method: Composition-based stats. Identities = 11/32 (34%), Positives = 18/32 (56%) Query: 102 QTLRNVDKAGTWAPRELVLVVQVHNRPEYLRL 133 +TLR+ DK G W E +++ PE ++L Sbjct: 595 ETLRDTDKFGRWGGEEFLIIAPQTGMPELMQL 626 >5GUW-B nrPDB Length = 465 Score = 28.8 bits (63), Expect = 4.8, Method: Composition-based stats. Identities = 10/31 (32%), Positives = 15/31 (48%), Gaps = 4/31 (12%) Query: 219 FGHYREAKFSQTKHHWWWKLHF----VWERV 245 F Y ++ K +WWW +H VWE + Sbjct: 187 FSFYNPENLTRDKFYWWWVVHLWVEGVWELI 217 >5GGG-A nrPDB Length = 578 Score = 28.4 bits (62), Expect = 5.8, Method: Composition-based stats. Identities = 58/286 (20%), Positives = 106/286 (37%), Gaps = 72/286 (25%) Query: 126 NRPEYLRLLLDSLRKAQGID-NVLVIFSHDFWSTEINQLIAGVNFCPVLQVFFPFSIQLY 184 NRP YL +L SL AQG+ ++ +F ++ ++ V+ +F IQ Sbjct: 219 NRPNYLYRMLRSLLSAQGVSPQMITVFIDGYYEEPMD----------VVALFGLRGIQHT 268 Query: 185 PNEFPGSDPRDCPRDLPKNAALKLGCINAEYPDSFGHYREAKFSQTKHHWWWKLHFVWER 244 P KNA + A +F + EAKF+ Sbjct: 269 PISI-------------KNARVSQH-YKASLTATFNLFPEAKFA---------------- 298 Query: 245 VKILRDYAGLILFLEEDHYLAPDFYHVFKKMWKLKQQECPECDVLSLGTYSASRSFYGMA 304 + LEED +A DF+ + L +++ D L + + + A Sbjct: 299 -----------VVLEEDLDIAVDFFSFLSQSIHLLEED----DSLYCISAWNDQGYEHTA 343 Query: 305 DKVDVKTWKSTEHNMGLALTRNAYQKLIECTDTFCTYDDYNWDWTLQYLTVSCLPKFWKV 364 + + T +G L R+ Y++ +E + T + WDW + ++ + + + Sbjct: 344 EDPALLYRVETMPGLGWVLRRSLYKEELE--PKWPTPEKL-WDWDM-WMRMPEQRRGREC 399 Query: 365 LVPQIPRIFHAGDCGMH------------HKKTCRPSTQSAQIESL 398 ++P + R +H G G++ HK P Q ++SL Sbjct: 400 IIPDVSRSYHFGIVGLNMNGYFHEAYFKKHKFNTVPGVQLRNVDSL 445 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.323 0.138 0.448 Lambda K H 0.267 0.0433 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 21,595,484 Number of Sequences: 36641 Number of extensions: 986178 Number of successful extensions: 1988 Number of sequences better than 10.0: 9 Number of HSP's better than 10.0 without gapping: 4 Number of HSP's successfully gapped in prelim test: 15 Number of HSP's that attempted gapping in prelim test: 1983 Number of HSP's gapped (non-prelim): 20 length of query: 447 length of database: 10,336,785 effective HSP length: 100 effective length of query: 347 effective length of database: 6,672,685 effective search space: 2315421695 effective search space used: 2315421695 T: 11 A: 40 X1: 16 ( 7.5 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.0 bits) S2: 61 (28.0 bits)