BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q08235 (291 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6EM4-A nrPDB 603 e-173 6CB1-b nrPDB 558 e-159 5WLC-SM nrPDB 35 0.048 3HL2-A nrPDB 31 0.54 2P9I-F nrPDB 30 1.2 1SP3-A nrPDB 30 1.8 3BC8-A nrPDB 29 2.4 4EQM-A nrPDB 29 3.0 6C0F-v nrPDB 28 5.9 4OUN-A nrPDB 27 8.2 >6EM4-A nrPDB Length = 291 Score = 603 bits (1556), Expect = e-173, Method: Compositional matrix adjust. Identities = 291/291 (100%), Positives = 291/291 (100%) Query: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR Sbjct: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 Query: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM Sbjct: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 Query: 121 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS 180 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS Sbjct: 121 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS 180 Query: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK Sbjct: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 Query: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK Sbjct: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 >6CB1-b nrPDB Length = 291 Score = 558 bits (1438), Expect = e-159, Method: Compositional matrix adjust. Identities = 273/291 (93%), Positives = 273/291 (93%) Query: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR Sbjct: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 Query: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM Sbjct: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 Query: 121 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS 180 DE GSRPVLSFDQRFESSPHYQLIKELLVHNFGVPP DHVMSFS Sbjct: 121 DEXXXXXXXXXGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPXXXXXXXXXDHVMSFS 180 Query: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK Sbjct: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 Query: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK Sbjct: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 >5WLC-SM nrPDB Length = 290 Score = 34.7 bits (78), Expect = 0.048, Method: Compositional matrix adjust. Identities = 35/133 (26%), Positives = 61/133 (45%), Gaps = 18/133 (13%) Query: 99 LWLSKPPNGPTIKFYIQNLHTMDELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVH 158 L +S P+GPT +F + N+ ++ GN + + P L FD F ++ +++ +L H Sbjct: 152 LTISHFPHGPTAQFSLHNVVMRHDIINAGNQSEVN-PHLIFDN-FTTALGKRVVC-ILKH 208 Query: 159 NFGVPPNARKSKPFIDHVMSFSIVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPR 218 F P K + V++F+ D I VR + Y E + + E+GPR Sbjct: 209 LFNAGP-----KKDSERVITFANRGDFISVRQH----------VYVRTREGVEIAEVGPR 253 Query: 219 FVMTVILILEGSF 231 F M + + G+ Sbjct: 254 FEMRLFELRLGTL 266 >3HL2-A nrPDB Length = 501 Score = 31.2 bits (69), Expect = 0.54, Method: Compositional matrix adjust. Identities = 20/50 (40%), Positives = 25/50 (50%), Gaps = 7/50 (14%) Query: 92 RKHQDLY-LWLSK---PPNG---PTIKFYIQNLHTMDELNFTGNCLKGSR 134 R H+ L L L K P NG T++ ++ L MD NF GNC G R Sbjct: 26 RSHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGVGER 75 >2P9I-F nrPDB Length = 168 Score = 30.0 bits (66), Expect = 1.2, Method: Compositional matrix adjust. Identities = 14/49 (28%), Positives = 26/49 (53%), Gaps = 1/49 (2%) Query: 71 LQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHT 119 ++Q +EI ++ C+ + F + ++ ++ KP G I F I N HT Sbjct: 76 VKQADEIEKIL-CHKFMRFMMMRAENFFILRRKPVEGYDISFLITNFHT 123 >1SP3-A nrPDB Length = 443 Score = 29.6 bits (65), Expect = 1.8, Method: Compositional matrix adjust. Identities = 30/120 (25%), Positives = 47/120 (39%), Gaps = 20/120 (16%) Query: 48 LIQDLSGLL---PHSRKEP--KLDTKKDLQQLNEIAELYNCNNVLFF----EARKHQDLY 98 + D +G P EP KLD K Q + NC + F+ +A KH DL Sbjct: 100 ICHDTTGTYVKDPAGAGEPMAKLDLAKIAQNVGAPVRD-NCGSCHFYGGGGDAVKHGDLD 158 Query: 99 LWLSKPPNGPTI-------KFYIQNLHTMDELNFTGNCLK---GSRPVLSFDQRFESSPH 148 ++ P + F QN HT ++ +GN + G + + +S+PH Sbjct: 159 SSMAYPDKATDVHMDSDGNNFQCQNCHTTEKHQISGNAMGVSPGGIDHIGCENCHDSAPH 218 >3BC8-A nrPDB Length = 450 Score = 28.9 bits (63), Expect = 2.4, Method: Compositional matrix adjust. Identities = 11/26 (42%), Positives = 15/26 (57%) Query: 109 TIKFYIQNLHTMDELNFTGNCLKGSR 134 T++ ++ L MD NF GNC G R Sbjct: 32 TLELFLHELAVMDSNNFLGNCGVGER 57 >4EQM-A nrPDB Length = 294 Score = 28.9 bits (63), Expect = 3.0, Method: Compositional matrix adjust. Identities = 30/117 (25%), Positives = 53/117 (45%), Gaps = 13/117 (11%) Query: 55 LLPHSRKEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYI 114 +P KE L K+ ++++ ++L + N V + + D Y + + GPT+ YI Sbjct: 45 FIPPREKEETL--KRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYI 102 Query: 115 QN---LHTMDELNFTGNCLKGSRPVLSFDQRF---ESSPHYQLI---KELLVHNFGV 162 ++ L +NFT L G + + D R + P LI K L + +FG+ Sbjct: 103 ESHGPLSVDTAINFTNQILDGIKH--AHDMRIVHRDIKPQNILIDSNKTLKIFDFGI 157 >6C0F-v nrPDB Length = 453 Score = 27.7 bits (60), Expect = 5.9, Method: Compositional matrix adjust. Identities = 15/49 (30%), Positives = 25/49 (51%) Query: 210 ISLVEIGPRFVMTVILILEGSFGGPKIYENKQYVSPNVVRAQIKQQAAE 258 I L E+GPR + ++ I EG G ++ S ++A K+ AA+ Sbjct: 326 IKLTELGPRLTLKLVKIEEGICSGKVLHHEFVQKSSEEIKALEKRHAAK 374 >4OUN-A nrPDB Length = 163 Score = 27.3 bits (59), Expect = 8.2, Method: Compositional matrix adjust. Identities = 17/51 (33%), Positives = 25/51 (49%), Gaps = 1/51 (1%) Query: 54 GLLPHSRKEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKP 104 GL+P + DT KD +QLN +A Y + +F +H L +KP Sbjct: 13 GLVPRGSHMLEFDTIKDSKQLNGLALAY-IGDAIFEVYVRHHLLKQGFTKP 62 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6EM4-A nrPDB 624 e-179 6CB1-b nrPDB 575 e-164 Sequences not found previously or not previously below threshold: 5WLC-SM nrPDB 33 0.13 3HL2-A nrPDB 31 0.48 6C0F-v nrPDB 30 1.3 4EQM-A nrPDB 29 2.6 3BC8-A nrPDB 29 2.7 CONVERGED! >6EM4-A nrPDB Length = 291 Score = 624 bits (1609), Expect = e-179, Method: Composition-based stats. Identities = 291/291 (100%), Positives = 291/291 (100%) Query: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR Sbjct: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 Query: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM Sbjct: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 Query: 121 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS 180 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS Sbjct: 121 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS 180 Query: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK Sbjct: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 Query: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK Sbjct: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 >6CB1-b nrPDB Length = 291 Score = 575 bits (1481), Expect = e-164, Method: Composition-based stats. Identities = 273/291 (93%), Positives = 273/291 (93%) Query: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR Sbjct: 1 MSSIYKALAGKSKDNKSEKKQGNVKQFMNKQRTLLISSRGVNYRHRHLIQDLSGLLPHSR 60 Query: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM Sbjct: 61 KEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYIQNLHTM 120 Query: 121 DELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPNARKSKPFIDHVMSFS 180 DE GSRPVLSFDQRFESSPHYQLIKELLVHNFGVPP DHVMSFS Sbjct: 121 DEXXXXXXXXXGSRPVLSFDQRFESSPHYQLIKELLVHNFGVPPXXXXXXXXXDHVMSFS 180 Query: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK Sbjct: 181 IVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPRFVMTVILILEGSFGGPKIYENK 240 Query: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK Sbjct: 241 QYVSPNVVRAQIKQQAAEEAKSRAEAAVERKIKRRENVLAADPLSNDALFK 291 >5WLC-SM nrPDB Length = 290 Score = 33.1 bits (74), Expect = 0.13, Method: Composition-based stats. Identities = 34/133 (25%), Positives = 58/133 (43%), Gaps = 18/133 (13%) Query: 99 LWLSKPPNGPTIKFYIQNLHTMDELNFTGNCLKGSRPVLSFDQRFESSPHYQLIKELLVH 158 L +S P+GPT +F + N+ ++ GN P L FD F ++ +++ +L H Sbjct: 152 LTISHFPHGPTAQFSLHNVVMRHDIINAGN-QSEVNPHLIFDN-FTTALGKRVVC-ILKH 208 Query: 159 NFGVPPNARKSKPFIDHVMSFSIVDDKIWVRTYEISHSTKNKEEYEDGEEDISLVEIGPR 218 F P + V++F+ D I VR + Y E + + E+GPR Sbjct: 209 LFNAGPKKDSER-----VITFANRGDFISVR----------QHVYVRTREGVEIAEVGPR 253 Query: 219 FVMTVILILEGSF 231 F M + + G+ Sbjct: 254 FEMRLFELRLGTL 266 >3HL2-A nrPDB Length = 501 Score = 31.2 bits (69), Expect = 0.48, Method: Composition-based stats. Identities = 20/53 (37%), Positives = 27/53 (50%), Gaps = 8/53 (15%) Query: 90 EARKHQD--LYLWLSK---PPNG---PTIKFYIQNLHTMDELNFTGNCLKGSR 134 EAR+ + + L L K P NG T++ ++ L MD NF GNC G R Sbjct: 23 EARRSHEHLIRLLLEKGKCPENGWDESTLELFLHELAIMDSNNFLGNCGVGER 75 >6C0F-v nrPDB Length = 453 Score = 30.0 bits (66), Expect = 1.3, Method: Composition-based stats. Identities = 21/69 (30%), Positives = 35/69 (50%), Gaps = 5/69 (7%) Query: 210 ISLVEIGPRFVMTVILILEGSFGGPKIYENKQYVSPNVVRAQIKQQAAEEAKSRAEAAVE 269 I L E+GPR + ++ I EG G ++ S ++A K+ A AK R + + Sbjct: 326 IKLTELGPRLTLKLVKIEEGICSGKVLHHEFVQKSSEEIKALEKRHA---AKMRLKE--Q 380 Query: 270 RKIKRRENV 278 RK ++ EN+ Sbjct: 381 RKKEQEENI 389 >4EQM-A nrPDB Length = 294 Score = 28.9 bits (63), Expect = 2.6, Method: Composition-based stats. Identities = 30/117 (25%), Positives = 53/117 (45%), Gaps = 13/117 (11%) Query: 55 LLPHSRKEPKLDTKKDLQQLNEIAELYNCNNVLFFEARKHQDLYLWLSKPPNGPTIKFYI 114 +P KE L K+ ++++ ++L + N V + + D Y + + GPT+ YI Sbjct: 45 FIPPREKEETL--KRFEREVHNSSQLSHQNIVSMIDVDEEDDCYYLVMEYIEGPTLSEYI 102 Query: 115 QN---LHTMDELNFTGNCLKGSRPVLSFDQRF---ESSPHYQLI---KELLVHNFGV 162 ++ L +NFT L G + + D R + P LI K L + +FG+ Sbjct: 103 ESHGPLSVDTAINFTNQILDGIKH--AHDMRIVHRDIKPQNILIDSNKTLKIFDFGI 157 >3BC8-A nrPDB Length = 450 Score = 28.9 bits (63), Expect = 2.7, Method: Composition-based stats. Identities = 19/53 (35%), Positives = 27/53 (50%), Gaps = 8/53 (15%) Query: 90 EARK-HQDLYLWLSK----PPNG---PTIKFYIQNLHTMDELNFTGNCLKGSR 134 EAR+ H+ L L + P +G T++ ++ L MD NF GNC G R Sbjct: 5 EARRAHEHLIRLLLEQGKCPEDGWDESTLELFLHELAVMDSNNFLGNCGVGER 57 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.316 0.133 0.382 Lambda K H 0.267 0.0416 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,453,424 Number of Sequences: 36641 Number of extensions: 460785 Number of successful extensions: 982 Number of sequences better than 10.0: 10 Number of HSP's better than 10.0 without gapping: 12 Number of HSP's successfully gapped in prelim test: 12 Number of HSP's that attempted gapping in prelim test: 966 Number of HSP's gapped (non-prelim): 26 length of query: 291 length of database: 10,336,785 effective HSP length: 96 effective length of query: 195 effective length of database: 6,819,249 effective search space: 1329753555 effective search space used: 1329753555 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 59 (27.3 bits)