BLASTP 2.2.23 [Feb-03-2010]


Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, 
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), 
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs",  Nucleic Acids Res. 25:3389-3402.


Reference for compositional score matrix adjustment: Altschul, Stephen F., 
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.


Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,  
Eugene V. Koonin, and Stephen F. Altschul (2001), 
"Improving the accuracy of PSI-BLAST protein database searches with 
composition-based statistics and other refinements",  Nucleic Acids Res. 29:2994-3005.

Query= Q08231
         (455 letters)

Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 
           36,641 sequences; 10,336,785 total letters

Searching..................................................done


Results from round 1


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value

3T5V-B nrPDB                                                          939   0.0  
5XXU-J nrPDB                                                           31   0.87 
6FYY-m nrPDB                                                           30   1.9  
6AZT-A nrPDB                                                           30   2.0  

>3T5V-B nrPDB
          Length = 455

 Score =  939 bits (2427), Expect = 0.0,   Method: Compositional matrix adjust.
 Identities = 455/455 (100%), Positives = 455/455 (100%)

Query: 1   MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTR 60
           MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTR
Sbjct: 1   MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTR 60

Query: 61  FKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFI 120
           FKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFI
Sbjct: 61  FKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFI 120

Query: 121 INLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVN 180
           INLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVN
Sbjct: 121 INLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVN 180

Query: 181 KLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAF 240
           KLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAF
Sbjct: 181 KLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAF 240

Query: 241 VQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWS 300
           VQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWS
Sbjct: 241 VQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWS 300

Query: 301 VLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQ 360
           VLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQ
Sbjct: 301 VLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQ 360

Query: 361 NKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQ 420
           NKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQ
Sbjct: 361 NKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQ 420

Query: 421 LQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW 455
           LQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW
Sbjct: 421 LQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW 455


>5XXU-J nrPDB
          Length = 188

 Score = 31.2 bits (69), Expect = 0.87,   Method: Compositional matrix adjust.
 Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 7/71 (9%)

Query: 211 FNEYQLDQQIEYRYLLGRYYLLNSQ----VHNAFVQFNEAFQSLLNLPLTNQAITRNGTR 266
           F + +LDQ+++   LLG Y L N +    V  A  +   A + LL L   +      GT 
Sbjct: 19  FEKERLDQEMK---LLGEYGLKNKREVWRVQYALAKIRSAARELLTLEEKDPRRVFQGTA 75

Query: 267 ILNYMIPTGLI 277
           +L  M+  GL+
Sbjct: 76  LLRRMVRLGLL 86


>6FYY-m nrPDB
          Length = 405

 Score = 30.4 bits (67), Expect = 1.9,   Method: Compositional matrix adjust.
 Identities = 12/46 (26%), Positives = 27/46 (58%), Gaps = 1/46 (2%)

Query: 390 IYNGIHSPKNVENVLVTLINLGLLRANCF-PQLQLCVVKKTTMIQE 434
           + NG+H P  +++VL   IN  +L  +C  P+ ++ + K   ++++
Sbjct: 75  LVNGVHEPAKLQDVLDGFINKFVLCGSCKNPETEIIITKDNDLVRD 120


>6AZT-A nrPDB
          Length = 491

 Score = 30.0 bits (66), Expect = 2.0,   Method: Compositional matrix adjust.
 Identities = 15/40 (37%), Positives = 22/40 (55%)

Query: 308 YGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLI 347
           Y N  G  +    NE HL A+ L+ VL +K  M TY+ ++
Sbjct: 175 YSNHGGPGVLGMPNEPHLVAKDLVDVLKKKHAMGTYKEMV 214


Searching..................................................done


Results from round 2


                                                                 Score    E
Sequences producing significant alignments:                      (bits) Value
Sequences used in model and found again:

3T5V-B nrPDB                                                          958   0.0  

Sequences not found previously or not previously below threshold:

4U0V-A nrPDB                                                           29   3.8  
5MRE-II nrPDB                                                          29   4.0  
5XXU-J nrPDB                                                           28   5.9  
6FYY-m nrPDB                                                           28   6.0  


CONVERGED!
>3T5V-B nrPDB
          Length = 455

 Score =  958 bits (2476), Expect = 0.0,   Method: Composition-based stats.
 Identities = 455/455 (100%), Positives = 455/455 (100%)

Query: 1   MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTR 60
           MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTR
Sbjct: 1   MDMANQLLDELAHGNFSHLTLNLSQNGREIAILQKQLTGFDDKQLETFVEQHPAMPNDTR 60

Query: 61  FKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFI 120
           FKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFI
Sbjct: 61  FKIMCTSFLNYARDVDPWSAWSSSDLIFEFYQCLINCLINDNAPHIEMLIPVATRETEFI 120

Query: 121 INLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVN 180
           INLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVN
Sbjct: 121 INLAGKLDSFHLQLHTRSHQFLSHISSILSRLFNSIKPPRGNASSTNIPGKQRILLYLVN 180

Query: 181 KLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAF 240
           KLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAF
Sbjct: 181 KLNNIYFRIESPQLCSNIFKNFQPKSMLAHFNEYQLDQQIEYRYLLGRYYLLNSQVHNAF 240

Query: 241 VQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWS 300
           VQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWS
Sbjct: 241 VQFNEAFQSLLNLPLTNQAITRNGTRILNYMIPTGLILGKMVKWGPLRPFLSQETIDNWS 300

Query: 301 VLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQ 360
           VLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQ
Sbjct: 301 VLYKHVRYGNIQGVSLWLRQNERHLCARQLLIVLLEKLPMVTYRNLIKTVIKSWTTEWGQ 360

Query: 361 NKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQ 420
           NKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQ
Sbjct: 361 NKLPYSLIERVLQLSIGPTFEDPGAQEITIYNGIHSPKNVENVLVTLINLGLLRANCFPQ 420

Query: 421 LQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW 455
           LQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW
Sbjct: 421 LQLCVVKKTTMIQEIVPPVNERITKMFPAHSHVLW 455


>4U0V-A nrPDB
          Length = 246

 Score = 29.2 bits (64), Expect = 3.8,   Method: Composition-based stats.
 Identities = 19/65 (29%), Positives = 29/65 (44%), Gaps = 3/65 (4%)

Query: 110 IPVATRETEFIINLAGKLDSFHLQLHTRSHQFLSHISSI-LSRLFNSIKPPRGNASSTNI 168
           IP+A   +     LAG+L+  H Q     H  +   +SI +SR    ++P        NI
Sbjct: 143 IPMAIESSHIPFELAGELNESHFQSSIYDH--IERYNSIPISRAKQELEPSAATTEEANI 200

Query: 169 PGKQR 173
            G Q+
Sbjct: 201 LGIQK 205


>5MRE-II nrPDB
          Length = 244

 Score = 29.2 bits (64), Expect = 4.0,   Method: Composition-based stats.
 Identities = 13/42 (30%), Positives = 28/42 (66%)

Query: 169 PGKQRILLYLVNKLNNIYFRIESPQLCSNIFKNFQPKSMLAH 210
           P +   LLY++NKL+NI  ++ + ++ S + + ++  SML++
Sbjct: 66  PTQYTQLLYMLNKLHNIDPQLTNDEITSELSQYYKKSSMLSN 107


>5XXU-J nrPDB
          Length = 188

 Score = 28.5 bits (62), Expect = 5.9,   Method: Composition-based stats.
 Identities = 22/71 (30%), Positives = 34/71 (47%), Gaps = 7/71 (9%)

Query: 211 FNEYQLDQQIEYRYLLGRYYLLNSQ----VHNAFVQFNEAFQSLLNLPLTNQAITRNGTR 266
           F + +LDQ+++   LLG Y L N +    V  A  +   A + LL L   +      GT 
Sbjct: 19  FEKERLDQEMK---LLGEYGLKNKREVWRVQYALAKIRSAARELLTLEEKDPRRVFQGTA 75

Query: 267 ILNYMIPTGLI 277
           +L  M+  GL+
Sbjct: 76  LLRRMVRLGLL 86


>6FYY-m nrPDB
          Length = 405

 Score = 28.5 bits (62), Expect = 6.0,   Method: Composition-based stats.
 Identities = 12/46 (26%), Positives = 27/46 (58%), Gaps = 1/46 (2%)

Query: 390 IYNGIHSPKNVENVLVTLINLGLLRANC-FPQLQLCVVKKTTMIQE 434
           + NG+H P  +++VL   IN  +L  +C  P+ ++ + K   ++++
Sbjct: 75  LVNGVHEPAKLQDVLDGFINKFVLCGSCKNPETEIIITKDNDLVRD 120


  Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
    Posted date:  Jan 3, 2023  9:05 PM
  Number of letters in database: 10,336,785
  Number of sequences in database:  36,641
  
Lambda     K      H
   0.323    0.138    0.420 

Lambda     K      H
   0.267   0.0434    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 19,928,565
Number of Sequences: 36641
Number of extensions: 866884
Number of successful extensions: 1716
Number of sequences better than 10.0: 7
Number of HSP's better than 10.0 without gapping: 7
Number of HSP's successfully gapped in prelim test: 6
Number of HSP's that attempted gapping in prelim test: 1709
Number of HSP's gapped (non-prelim): 13
length of query: 455
length of database: 10,336,785
effective HSP length: 100
effective length of query: 355
effective length of database: 6,672,685
effective search space: 2368803175
effective search space used: 2368803175
T: 11
A: 40
X1: 16 ( 7.5 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.0 bits)
S2: 61 (28.0 bits)