BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q08024 (187 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4N9F-a nrPDB 338 7e-94 2JHB-A nrPDB 295 6e-81 3HKO-A nrPDB 30 0.41 5ODN-A nrPDB 28 2.3 4AQ4-A nrPDB 28 2.7 6HV9-A nrPDB 28 3.0 6FF7-0 nrPDB 27 3.6 3ACO-A nrPDB 27 4.5 3HAJ-A nrPDB 27 4.7 3LLL-A nrPDB 27 5.0 >4N9F-a nrPDB Length = 170 Score = 338 bits (868), Expect = 7e-94, Method: Compositional matrix adjust. Identities = 165/170 (97%), Positives = 165/170 (97%) Query: 1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT 60 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQ RFQNACRDGRSEIAFVAT Sbjct: 1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVAT 60 Query: 61 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD 120 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDL RLD Sbjct: 61 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLD 120 Query: 121 GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQ 170 GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEME R Q Sbjct: 121 GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQ 170 >2JHB-A nrPDB Length = 143 Score = 295 bits (756), Expect = 6e-81, Method: Compositional matrix adjust. Identities = 141/141 (100%), Positives = 141/141 (100%) Query: 1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT 60 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT Sbjct: 3 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT 62 Query: 61 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD 120 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD Sbjct: 63 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD 122 Query: 121 GMGCLEFDEERAQQEDALAQQ 141 GMGCLEFDEERAQQEDALAQQ Sbjct: 123 GMGCLEFDEERAQQEDALAQQ 143 >3HKO-A nrPDB Length = 345 Score = 30.4 bits (67), Expect = 0.41, Method: Compositional matrix adjust. Identities = 24/91 (26%), Positives = 36/91 (39%), Gaps = 9/91 (9%) Query: 45 QNAC-RDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMIL 103 Q C RD + E +T + ++ E + + EY + +AG Y AP +L Sbjct: 187 QGICHRDIKPENFLFSTNKSFEIKLVDFGLSKEFYKLNNGEYYGMTTKAGTPYFVAPEVL 246 Query: 104 NGV-------CVIWKGWIDLHRLDGMGCLEF 127 N C W + LH L MG + F Sbjct: 247 NTTNESYGPKCDAWSAGVLLHLLL-MGAVPF 276 >5ODN-A nrPDB Length = 196 Score = 28.1 bits (61), Expect = 2.3, Method: Compositional matrix adjust. Identities = 16/46 (34%), Positives = 24/46 (52%) Query: 139 AQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGGGDDL 184 Q + E + +TR +EDRD + R E + Q+ SN GG D+ Sbjct: 101 GSQVYFEGKLQTRSWEDRDNNTRYSTEVKAQEMMFLDSNRQGGADM 146 >4AQ4-A nrPDB Length = 419 Score = 28.1 bits (61), Expect = 2.7, Method: Compositional matrix adjust. Identities = 16/47 (34%), Positives = 20/47 (42%), Gaps = 2/47 (4%) Query: 81 PSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEF 127 P + + DL A K LKA + G W+GWI L L F Sbjct: 146 PPKTWQDLADYAAK--LKASGMKCGYASGWQGWIQLENFSAWNGLPF 190 >6HV9-A nrPDB Length = 914 Score = 27.7 bits (60), Expect = 3.0, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 23/45 (51%), Gaps = 2/45 (4%) Query: 113 WIDLHRLDGMGCLEFDEERAQQEDALAQQAFEE--ARRRTREFED 155 W+D G+ C+E +E+ Q A++Q ++ + EFED Sbjct: 604 WMDKFNFSGLACIEIEEKENQDYTAVSQWQLKKFLSPIYQPEFED 648 >6FF7-0 nrPDB Length = 396 Score = 27.3 bits (59), Expect = 3.6, Method: Compositional matrix adjust. Identities = 22/56 (39%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Query: 128 DEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGGGDD 183 D R +ED ++ E+ RR R DRDR HR RR + PGS G G D Sbjct: 113 DHPRRGREDRQHREPSEQEHRRARN-SDRDR-HRGHSHQRRTSNERPGSGQGQGRD 166 >3ACO-A nrPDB Length = 350 Score = 27.3 bits (59), Expect = 4.5, Method: Compositional matrix adjust. Identities = 20/36 (55%), Positives = 21/36 (58%), Gaps = 5/36 (13%) Query: 115 DLHRL--DGMGCLEFDEERAQQEDALAQQAFEEARR 148 D HRL D M CL ERA+ E A AQQ E ARR Sbjct: 40 DGHRLCSDLMNCLH---ERARIEKAYAQQLTEWARR 72 >3HAJ-A nrPDB Length = 486 Score = 26.9 bits (58), Expect = 4.7, Method: Compositional matrix adjust. Identities = 20/36 (55%), Positives = 21/36 (58%), Gaps = 5/36 (13%) Query: 115 DLHRL--DGMGCLEFDEERAQQEDALAQQAFEEARR 148 D HRL D M CL ERA+ E A AQQ E ARR Sbjct: 33 DGHRLCSDLMNCLH---ERARIEKAYAQQLTEWARR 65 >3LLL-A nrPDB Length = 287 Score = 26.9 bits (58), Expect = 5.0, Method: Compositional matrix adjust. Identities = 20/36 (55%), Positives = 21/36 (58%), Gaps = 5/36 (13%) Query: 115 DLHRL--DGMGCLEFDEERAQQEDALAQQAFEEARR 148 D HRL D M CL ERA+ E A AQQ E ARR Sbjct: 18 DGHRLCGDLMNCLH---ERARIEKAYAQQLTEWARR 50 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4N9F-a nrPDB 312 7e-86 2JHB-A nrPDB 281 2e-76 Sequences not found previously or not previously below threshold: 5ODN-A nrPDB 32 0.13 6FF7-0 nrPDB 32 0.14 2I1J-A nrPDB 29 1.1 4QXA-A nrPDB 29 1.2 3HKO-A nrPDB 28 1.8 4AQ4-A nrPDB 28 2.0 6HV9-A nrPDB 28 2.3 3HAJ-A nrPDB 27 7.2 3JCR-F nrPDB 26 8.3 3ACO-A nrPDB 26 9.2 CONVERGED! >4N9F-a nrPDB Length = 170 Score = 312 bits (799), Expect = 7e-86, Method: Composition-based stats. Identities = 165/170 (97%), Positives = 165/170 (97%) Query: 1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT 60 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQ RFQNACRDGRSEIAFVAT Sbjct: 1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQARFQNACRDGRSEIAFVAT 60 Query: 61 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD 120 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDL RLD Sbjct: 61 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLQRLD 120 Query: 121 GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQ 170 GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEME R Q Sbjct: 121 GMGCLEFDEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEVRVSQ 170 >2JHB-A nrPDB Length = 143 Score = 281 bits (718), Expect = 2e-76, Method: Composition-based stats. Identities = 141/141 (100%), Positives = 141/141 (100%) Query: 1 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT 60 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT Sbjct: 3 MPRVVPDQRSKFENEEFFRKLSRECEIKYTGFRDRPHEERQTRFQNACRDGRSEIAFVAT 62 Query: 61 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD 120 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD Sbjct: 63 GTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLD 122 Query: 121 GMGCLEFDEERAQQEDALAQQ 141 GMGCLEFDEERAQQEDALAQQ Sbjct: 123 GMGCLEFDEERAQQEDALAQQ 143 >5ODN-A nrPDB Length = 196 Score = 32.3 bits (72), Expect = 0.13, Method: Composition-based stats. Identities = 16/46 (34%), Positives = 24/46 (52%) Query: 139 AQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGGGDDL 184 Q + E + +TR +EDRD + R E + Q+ SN GG D+ Sbjct: 101 GSQVYFEGKLQTRSWEDRDNNTRYSTEVKAQEMMFLDSNRQGGADM 146 >6FF7-0 nrPDB Length = 396 Score = 32.3 bits (72), Expect = 0.14, Method: Composition-based stats. Identities = 22/56 (39%), Positives = 27/56 (48%), Gaps = 2/56 (3%) Query: 128 DEERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNLGGGDD 183 D R +ED ++ E+ RR R DRDR HR RR + PGS G G D Sbjct: 113 DHPRRGREDRQHREPSEQEHRRARN-SDRDR-HRGHSHQRRTSNERPGSGQGQGRD 166 >2I1J-A nrPDB Length = 575 Score = 29.2 bits (64), Expect = 1.1, Method: Composition-based stats. Identities = 25/75 (33%), Positives = 39/75 (52%), Gaps = 8/75 (10%) Query: 114 IDLHRLDGMGCLEFDEERAQQED-ALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDP 172 ID+ ++ E ++AQ+E L A E A ++ +E++DR R +EEME R Q Sbjct: 300 IDVQQMKAQAREEKLAKQAQREKLQLEIAARERAEKKQQEYQDRLRQMQEEME--RSQ-- 355 Query: 173 SPGSNLGGGDDLKLR 187 +NL D+ LR Sbjct: 356 ---ANLLEAQDMILR 367 >4QXA-A nrPDB Length = 208 Score = 29.2 bits (64), Expect = 1.2, Method: Composition-based stats. Identities = 15/49 (30%), Positives = 21/49 (42%) Query: 130 ERAQQEDALAQQAFEEARRRTREFEDRDRSHREEMEARRQQDPSPGSNL 178 E + ++ AFEEA RR EDR + + P P S+L Sbjct: 153 ETSAKDSTNVAAAFEEAVRRILATEDRSEHLIQTDTVNLHRKPKPNSSL 201 >3HKO-A nrPDB Length = 345 Score = 28.4 bits (62), Expect = 1.8, Method: Composition-based stats. Identities = 24/91 (26%), Positives = 36/91 (39%), Gaps = 9/91 (9%) Query: 45 QNAC-RDGRSEIAFVATGTNLSLQFFPASWQGEQRQTPSREYVDLEREAGKVYLKAPMIL 103 Q C RD + E +T + ++ E + + EY + +AG Y AP +L Sbjct: 187 QGICHRDIKPENFLFSTNKSFEIKLVDFGLSKEFYKLNNGEYYGMTTKAGTPYFVAPEVL 246 Query: 104 NGV-------CVIWKGWIDLHRLDGMGCLEF 127 N C W + LH L MG + F Sbjct: 247 NTTNESYGPKCDAWSAGVLLHLLL-MGAVPF 276 >4AQ4-A nrPDB Length = 419 Score = 28.4 bits (62), Expect = 2.0, Method: Composition-based stats. Identities = 17/53 (32%), Positives = 22/53 (41%), Gaps = 2/53 (3%) Query: 75 GEQRQTPSREYVDLEREAGKVYLKAPMILNGVCVIWKGWIDLHRLDGMGCLEF 127 G + P + + DL A K LKA + G W+GWI L L F Sbjct: 140 GLDPEQPPKTWQDLADYAAK--LKASGMKCGYASGWQGWIQLENFSAWNGLPF 190 >6HV9-A nrPDB Length = 914 Score = 28.0 bits (61), Expect = 2.3, Method: Composition-based stats. Identities = 13/45 (28%), Positives = 23/45 (51%), Gaps = 2/45 (4%) Query: 113 WIDLHRLDGMGCLEFDEERAQQEDALAQQAFEE--ARRRTREFED 155 W+D G+ C+E +E+ Q A++Q ++ + EFED Sbjct: 604 WMDKFNFSGLACIEIEEKENQDYTAVSQWQLKKFLSPIYQPEFED 648 >3HAJ-A nrPDB Length = 486 Score = 26.5 bits (57), Expect = 7.2, Method: Composition-based stats. Identities = 20/36 (55%), Positives = 21/36 (58%), Gaps = 5/36 (13%) Query: 115 DLHRL--DGMGCLEFDEERAQQEDALAQQAFEEARR 148 D HRL D M CL ERA+ E A AQQ E ARR Sbjct: 33 DGHRLCSDLMNCLH---ERARIEKAYAQQLTEWARR 65 >3JCR-F nrPDB Length = 820 Score = 26.1 bits (56), Expect = 8.3, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 20/34 (58%) Query: 130 ERAQQEDALAQQAFEEARRRTREFEDRDRSHREE 163 +R QQE Q+ EE R++ ++F+D R E+ Sbjct: 175 KRRQQEVEERQRMLEEERKKRKQFQDLGRKMLED 208 >3ACO-A nrPDB Length = 350 Score = 26.1 bits (56), Expect = 9.2, Method: Composition-based stats. Identities = 20/36 (55%), Positives = 21/36 (58%), Gaps = 5/36 (13%) Query: 115 DLHRL--DGMGCLEFDEERAQQEDALAQQAFEEARR 148 D HRL D M CL ERA+ E A AQQ E ARR Sbjct: 40 DGHRLCSDLMNCLH---ERARIEKAYAQQLTEWARR 72 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.309 0.139 0.435 Lambda K H 0.267 0.0415 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 7,740,531 Number of Sequences: 36641 Number of extensions: 306294 Number of successful extensions: 761 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 15 Number of HSP's successfully gapped in prelim test: 20 Number of HSP's that attempted gapping in prelim test: 738 Number of HSP's gapped (non-prelim): 45 length of query: 187 length of database: 10,336,785 effective HSP length: 91 effective length of query: 96 effective length of database: 7,002,454 effective search space: 672235584 effective search space used: 672235584 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.1 bits) S2: 56 (26.2 bits)