BLASTP 2.2.23 [Feb-03-2010]
Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer,
Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997),
"Gapped BLAST and PSI-BLAST: a new generation of protein database search
programs", Nucleic Acids Res. 25:3389-3402.
Reference for compositional score matrix adjustment: Altschul, Stephen F.,
John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis,
Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches
using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109.
Reference for composition-based statistics starting in round 2:
Schaffer, Alejandro A., L. Aravind, Thomas L. Madden,
Sergei Shavirin, John L. Spouge, Yuri I. Wolf,
Eugene V. Koonin, and Stephen F. Altschul (2001),
"Improving the accuracy of PSI-BLAST protein database searches with
composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005.
Query= Q07508
(261 letters)
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
36,641 sequences; 10,336,785 total letters
Searching..................................................done
Results from round 1
Score E
Sequences producing significant alignments: (bits) Value
5ZWN-Y nrPDB 543 e-155
6G90-H nrPDB 402 e-112
6E53-A nrPDB 30 0.91
2Q14-A nrPDB 30 1.4
1RQL-A nrPDB 29 1.9
3QOD-A nrPDB 28 2.8
5IP1-A nrPDB 28 2.8
2CHV-A nrPDB 27 7.4
>5ZWN-Y nrPDB
Length = 261
Score = 543 bits (1400), Expect = e-155, Method: Compositional matrix adjust.
Identities = 261/261 (100%), Positives = 261/261 (100%)
Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG
Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120
TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK
Sbjct: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120
Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA
Sbjct: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM
Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
Query: 241 KNNRTTNASKTATTLPGRRFV 261
KNNRTTNASKTATTLPGRRFV
Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261
>6G90-H nrPDB
Length = 261
Score = 402 bits (1032), Expect = e-112, Method: Compositional matrix adjust.
Identities = 203/261 (77%), Positives = 203/261 (77%)
Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG
Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120
TKQSLGKCPQMHLTKHKIQYER KQGKTF
Sbjct: 61 TKQSLGKCPQMHLTKHKIQYERVEKQGKTFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120
Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
GQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM
Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
Query: 241 KNNRTTNASKTATTLPGRRFV 261
KNNRTTNASKTATTLPGRRFV
Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261
>6E53-A nrPDB
Length = 596
Score = 30.4 bits (67), Expect = 0.91, Method: Compositional matrix adjust.
Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 12/69 (17%)
Query: 200 VCEVCGA-----YLSRLDTD----RRLADHFLGKIHLGYVKMREDYDRLMKNNRTTNASK 250
+CE+ A Y S LD D R + D+F H V D++ L+K N +K
Sbjct: 316 LCELYMAFMDRLYFSNLDKDAFIHRTVDDYFFCSPHPHKV---YDFELLIKGVYQVNPTK 372
Query: 251 TATTLPGRR 259
T T LP R
Sbjct: 373 TRTNLPTHR 381
>2Q14-A nrPDB
Length = 410
Score = 29.6 bits (65), Expect = 1.4, Method: Compositional matrix adjust.
Identities = 22/74 (29%), Positives = 35/74 (47%), Gaps = 9/74 (12%)
Query: 65 LGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLKHTAE 124
+G P H+ E + QG + E L ++ R E NGQ+S+A+Q K
Sbjct: 98 IGHGPFSHVL------EDTIVQGVSHEEIS---LMLMERMNKEMNGQLSLAIQIFKDEYP 148
Query: 125 ERMKIQQVTEELDV 138
+R Q V+ +LD+
Sbjct: 149 KRFLHQLVSGQLDM 162
>1RQL-A nrPDB
Length = 267
Score = 29.3 bits (64), Expect = 1.9, Method: Compositional matrix adjust.
Identities = 27/107 (25%), Positives = 50/107 (46%), Gaps = 12/107 (11%)
Query: 63 QSLGKCPQMHLTKHKI--QYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120
++L + P++ +++ Q E + + EFE AIL R+ + N A++ +
Sbjct: 58 RALTEMPRIASEWNRVFRQLPTEADIQEMYEEFEEILFAILPRYASPIN-----AVKEVI 112
Query: 121 HTAEER-MKIQQVT----EELDVLDVRIGLMGQEIDSLIRADEVSMG 162
+ ER +KI T E +D++ L G + D L+ D+V G
Sbjct: 113 ASLRERGIKIGSTTGYTREMMDIVAKEAALQGYKPDFLVTPDDVPAG 159
>3QOD-A nrPDB
Length = 302
Score = 28.5 bits (62), Expect = 2.8, Method: Compositional matrix adjust.
Identities = 14/38 (36%), Positives = 23/38 (60%)
Query: 119 LKHTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRA 156
++ AE+R +V EELD+L ++ E+D +IRA
Sbjct: 241 MRDWAEKRPNTMRVLEELDILPEKMQQAKDELDEIIRA 278
>5IP1-A nrPDB
Length = 279
Score = 28.5 bits (62), Expect = 2.8, Method: Compositional matrix adjust.
Identities = 30/88 (34%), Positives = 43/88 (48%), Gaps = 24/88 (27%)
Query: 35 RDLGLHDPKICKSYLVGECPYDLFQGTKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFE 94
+DLG+ DPK YD TK++LGK + +K E +VK+GK
Sbjct: 196 KDLGI-DPK----------KYD----TKEALGKVCTVLKSKAFEMNEDQVKKGK------ 234
Query: 95 REYLAILSRFVNECNGQISVALQNLKHT 122
EY AILS + N + SVA+++ T
Sbjct: 235 -EYAAILSS--SNPNAKGSVAMEHYSET 259
>2CHV-A nrPDB
Length = 319
Score = 27.3 bits (59), Expect = 7.4, Method: Compositional matrix adjust.
Identities = 26/80 (32%), Positives = 43/80 (53%), Gaps = 12/80 (15%)
Query: 134 EELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVAKRVRNITENVGQS 193
E LD L V G+ G++I + + + +SM + S+K+Q LI K EV R+ +
Sbjct: 248 ELLDRLMVEYGMSGEDIVAQLFREIISMPIKDSLKVQ-LIDKLGEVDFRLT-------EG 299
Query: 194 AQQKLQVCEVCGAYLSRLDT 213
A +++Q+ AYL+ L T
Sbjct: 300 ANERIQL----DAYLAYLST 315
Searching..................................................done
Results from round 2
Score E
Sequences producing significant alignments: (bits) Value
Sequences used in model and found again:
5ZWN-Y nrPDB 440 e-124
6G90-H nrPDB 358 1e-99
Sequences not found previously or not previously below threshold:
3CS1-A nrPDB 28 5.3
5NP8-A nrPDB 28 5.6
CONVERGED!
>5ZWN-Y nrPDB
Length = 261
Score = 440 bits (1132), Expect = e-124, Method: Composition-based stats.
Identities = 261/261 (100%), Positives = 261/261 (100%)
Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG
Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120
TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK
Sbjct: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120
Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA
Sbjct: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM
Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
Query: 241 KNNRTTNASKTATTLPGRRFV 261
KNNRTTNASKTATTLPGRRFV
Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261
>6G90-H nrPDB
Length = 261
Score = 358 bits (919), Expect = 1e-99, Method: Composition-based stats.
Identities = 203/261 (77%), Positives = 203/261 (77%)
Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG
Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60
Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120
TKQSLGKCPQMHLTKHKIQYER KQGKTF
Sbjct: 61 TKQSLGKCPQMHLTKHKIQYERVEKQGKTFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120
Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
GQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA
Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180
Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM
Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240
Query: 241 KNNRTTNASKTATTLPGRRFV 261
KNNRTTNASKTATTLPGRRFV
Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261
>3CS1-A nrPDB
Length = 219
Score = 27.6 bits (60), Expect = 5.3, Method: Composition-based stats.
Identities = 13/41 (31%), Positives = 23/41 (56%)
Query: 157 DEVSMGMLQSVKLQELISKRKEVAKRVRNITENVGQSAQQK 197
DEV G L+ +KL E S+ +++ KR + + +G + K
Sbjct: 68 DEVYSGCLEVLKLDEFTSRVRDITKRAFDKSRTLGSKLENK 108
>5NP8-A nrPDB
Length = 420
Score = 27.6 bits (60), Expect = 5.6, Method: Composition-based stats.
Identities = 12/31 (38%), Positives = 18/31 (58%)
Query: 230 VKMREDYDRLMKNNRTTNASKTATTLPGRRF 260
V MR D++ MKNN+ TN + +P +F
Sbjct: 22 VVMRVDFNVPMKNNQITNNQRIKAAVPSIKF 52
Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta
Posted date: Jan 3, 2023 9:05 PM
Number of letters in database: 10,336,785
Number of sequences in database: 36,641
Lambda K H
0.336 0.141 0.444
Lambda K H
0.267 0.0425 0.140
Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Hits to DB: 11,838,284
Number of Sequences: 36641
Number of extensions: 549117
Number of successful extensions: 1559
Number of sequences better than 10.0: 31
Number of HSP's better than 10.0 without gapping: 26
Number of HSP's successfully gapped in prelim test: 25
Number of HSP's that attempted gapping in prelim test: 1528
Number of HSP's gapped (non-prelim): 56
length of query: 261
length of database: 10,336,785
effective HSP length: 95
effective length of query: 166
effective length of database: 6,855,890
effective search space: 1138077740
effective search space used: 1138077740
T: 11
A: 40
X1: 16 ( 7.8 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (22.7 bits)
S2: 58 (26.9 bits)