BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q07508 (261 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5ZWN-Y nrPDB 543 e-155 6G90-H nrPDB 402 e-112 6E53-A nrPDB 30 0.91 2Q14-A nrPDB 30 1.4 1RQL-A nrPDB 29 1.9 3QOD-A nrPDB 28 2.8 5IP1-A nrPDB 28 2.8 2CHV-A nrPDB 27 7.4 >5ZWN-Y nrPDB Length = 261 Score = 543 bits (1400), Expect = e-155, Method: Compositional matrix adjust. Identities = 261/261 (100%), Positives = 261/261 (100%) Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK Sbjct: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120 Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA Sbjct: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 Query: 241 KNNRTTNASKTATTLPGRRFV 261 KNNRTTNASKTATTLPGRRFV Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261 >6G90-H nrPDB Length = 261 Score = 402 bits (1032), Expect = e-112, Method: Compositional matrix adjust. Identities = 203/261 (77%), Positives = 203/261 (77%) Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120 TKQSLGKCPQMHLTKHKIQYER KQGKTF Sbjct: 61 TKQSLGKCPQMHLTKHKIQYERVEKQGKTFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120 Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 GQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 Query: 241 KNNRTTNASKTATTLPGRRFV 261 KNNRTTNASKTATTLPGRRFV Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261 >6E53-A nrPDB Length = 596 Score = 30.4 bits (67), Expect = 0.91, Method: Compositional matrix adjust. Identities = 23/69 (33%), Positives = 31/69 (44%), Gaps = 12/69 (17%) Query: 200 VCEVCGA-----YLSRLDTD----RRLADHFLGKIHLGYVKMREDYDRLMKNNRTTNASK 250 +CE+ A Y S LD D R + D+F H V D++ L+K N +K Sbjct: 316 LCELYMAFMDRLYFSNLDKDAFIHRTVDDYFFCSPHPHKV---YDFELLIKGVYQVNPTK 372 Query: 251 TATTLPGRR 259 T T LP R Sbjct: 373 TRTNLPTHR 381 >2Q14-A nrPDB Length = 410 Score = 29.6 bits (65), Expect = 1.4, Method: Compositional matrix adjust. Identities = 22/74 (29%), Positives = 35/74 (47%), Gaps = 9/74 (12%) Query: 65 LGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLKHTAE 124 +G P H+ E + QG + E L ++ R E NGQ+S+A+Q K Sbjct: 98 IGHGPFSHVL------EDTIVQGVSHEEIS---LMLMERMNKEMNGQLSLAIQIFKDEYP 148 Query: 125 ERMKIQQVTEELDV 138 +R Q V+ +LD+ Sbjct: 149 KRFLHQLVSGQLDM 162 >1RQL-A nrPDB Length = 267 Score = 29.3 bits (64), Expect = 1.9, Method: Compositional matrix adjust. Identities = 27/107 (25%), Positives = 50/107 (46%), Gaps = 12/107 (11%) Query: 63 QSLGKCPQMHLTKHKI--QYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120 ++L + P++ +++ Q E + + EFE AIL R+ + N A++ + Sbjct: 58 RALTEMPRIASEWNRVFRQLPTEADIQEMYEEFEEILFAILPRYASPIN-----AVKEVI 112 Query: 121 HTAEER-MKIQQVT----EELDVLDVRIGLMGQEIDSLIRADEVSMG 162 + ER +KI T E +D++ L G + D L+ D+V G Sbjct: 113 ASLRERGIKIGSTTGYTREMMDIVAKEAALQGYKPDFLVTPDDVPAG 159 >3QOD-A nrPDB Length = 302 Score = 28.5 bits (62), Expect = 2.8, Method: Compositional matrix adjust. Identities = 14/38 (36%), Positives = 23/38 (60%) Query: 119 LKHTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRA 156 ++ AE+R +V EELD+L ++ E+D +IRA Sbjct: 241 MRDWAEKRPNTMRVLEELDILPEKMQQAKDELDEIIRA 278 >5IP1-A nrPDB Length = 279 Score = 28.5 bits (62), Expect = 2.8, Method: Compositional matrix adjust. Identities = 30/88 (34%), Positives = 43/88 (48%), Gaps = 24/88 (27%) Query: 35 RDLGLHDPKICKSYLVGECPYDLFQGTKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFE 94 +DLG+ DPK YD TK++LGK + +K E +VK+GK Sbjct: 196 KDLGI-DPK----------KYD----TKEALGKVCTVLKSKAFEMNEDQVKKGK------ 234 Query: 95 REYLAILSRFVNECNGQISVALQNLKHT 122 EY AILS + N + SVA+++ T Sbjct: 235 -EYAAILSS--SNPNAKGSVAMEHYSET 259 >2CHV-A nrPDB Length = 319 Score = 27.3 bits (59), Expect = 7.4, Method: Compositional matrix adjust. Identities = 26/80 (32%), Positives = 43/80 (53%), Gaps = 12/80 (15%) Query: 134 EELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVAKRVRNITENVGQS 193 E LD L V G+ G++I + + + +SM + S+K+Q LI K EV R+ + Sbjct: 248 ELLDRLMVEYGMSGEDIVAQLFREIISMPIKDSLKVQ-LIDKLGEVDFRLT-------EG 299 Query: 194 AQQKLQVCEVCGAYLSRLDT 213 A +++Q+ AYL+ L T Sbjct: 300 ANERIQL----DAYLAYLST 315 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5ZWN-Y nrPDB 440 e-124 6G90-H nrPDB 358 1e-99 Sequences not found previously or not previously below threshold: 3CS1-A nrPDB 28 5.3 5NP8-A nrPDB 28 5.6 CONVERGED! >5ZWN-Y nrPDB Length = 261 Score = 440 bits (1132), Expect = e-124, Method: Composition-based stats. Identities = 261/261 (100%), Positives = 261/261 (100%) Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK Sbjct: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120 Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA Sbjct: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 Query: 241 KNNRTTNASKTATTLPGRRFV 261 KNNRTTNASKTATTLPGRRFV Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261 >6G90-H nrPDB Length = 261 Score = 358 bits (919), Expect = 1e-99, Method: Composition-based stats. Identities = 203/261 (77%), Positives = 203/261 (77%) Query: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG Sbjct: 1 MSTMSTPAAEQRKLVEQLMGRDFSFRHNRYSHQKRDLGLHDPKICKSYLVGECPYDLFQG 60 Query: 61 TKQSLGKCPQMHLTKHKIQYEREVKQGKTFPEFEREYLAILSRFVNECNGQISVALQNLK 120 TKQSLGKCPQMHLTKHKIQYER KQGKTF Sbjct: 61 TKQSLGKCPQMHLTKHKIQYERVEKQGKTFXXXXXXXXXXXXXXXXXXXXXXXXXXXXXX 120 Query: 121 HTAEERMKIQQVTEELDVLDVRIGLMGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 GQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA Sbjct: 121 XXXXXXXXXXXXXXXXXXXXXXXXXXGQEIDSLIRADEVSMGMLQSVKLQELISKRKEVA 180 Query: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM Sbjct: 181 KRVRNITENVGQSAQQKLQVCEVCGAYLSRLDTDRRLADHFLGKIHLGYVKMREDYDRLM 240 Query: 241 KNNRTTNASKTATTLPGRRFV 261 KNNRTTNASKTATTLPGRRFV Sbjct: 241 KNNRTTNASKTATTLPGRRFV 261 >3CS1-A nrPDB Length = 219 Score = 27.6 bits (60), Expect = 5.3, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%) Query: 157 DEVSMGMLQSVKLQELISKRKEVAKRVRNITENVGQSAQQK 197 DEV G L+ +KL E S+ +++ KR + + +G + K Sbjct: 68 DEVYSGCLEVLKLDEFTSRVRDITKRAFDKSRTLGSKLENK 108 >5NP8-A nrPDB Length = 420 Score = 27.6 bits (60), Expect = 5.6, Method: Composition-based stats. Identities = 12/31 (38%), Positives = 18/31 (58%) Query: 230 VKMREDYDRLMKNNRTTNASKTATTLPGRRF 260 V MR D++ MKNN+ TN + +P +F Sbjct: 22 VVMRVDFNVPMKNNQITNNQRIKAAVPSIKF 52 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.336 0.141 0.444 Lambda K H 0.267 0.0425 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 11,838,284 Number of Sequences: 36641 Number of extensions: 549117 Number of successful extensions: 1559 Number of sequences better than 10.0: 31 Number of HSP's better than 10.0 without gapping: 26 Number of HSP's successfully gapped in prelim test: 25 Number of HSP's that attempted gapping in prelim test: 1528 Number of HSP's gapped (non-prelim): 56 length of query: 261 length of database: 10,336,785 effective HSP length: 95 effective length of query: 166 effective length of database: 6,855,890 effective search space: 1138077740 effective search space used: 1138077740 T: 11 A: 40 X1: 16 ( 7.8 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (22.7 bits) S2: 58 (26.9 bits)