BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q07307 (574 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 5I6C-A nrPDB 1162 0.0 3QE7-A nrPDB 75 1e-13 >5I6C-A nrPDB Length = 574 Score = 1162 bits (3006), Expect = 0.0, Method: Compositional matrix adjust. Identities = 573/574 (99%), Positives = 573/574 (99%) Query: 1 MDNSIHSTDGPDSVIPNSNPKKTVRQRVRLLARHLTTREGLIGDYDYGFLFRPELPFMKK 60 MDNSIHSTDGPDSVIPNSNPKKTVRQRVRLLARHLTTREGLIGDYDYGFLFRPELPFMKK Sbjct: 1 MDNSIHSTDGPDSVIPNSNPKKTVRQRVRLLARHLTTREGLIGDYDYGFLFRPELPFMKK 60 Query: 61 DPRAPPFFGLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSL 120 DPRAPPFFGLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSL Sbjct: 61 DPRAPPFFGLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSL 120 Query: 121 IVCGLLSMVQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAG 180 IVCGLLSMVQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAG Sbjct: 121 IVCGLLSMVQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAG 180 Query: 181 NRLPCPEAYGALIGTSACCALVEILLAFVPPKVIQKIFPPIVTGPTVMLIGISLIGTGFK 240 NRLPCPEAYGALIGTSACCALVEILLAFVPPKVIQKIFPPIVTGPTVMLIGISLIGTGFK Sbjct: 181 NRLPCPEAYGALIGTSACCALVEILLAFVPPKVIQKIFPPIVTGPTVMLIGISLIGTGFK 240 Query: 241 DWAGGSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVI 300 DWAGGSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVI Sbjct: 241 DWAGGSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVI 300 Query: 301 GLLVGCIVAAACGYFSHADIDAAPAASFIWVKTFPLSVYGPMVLPIIAVFIICACECIGD 360 GLLVGCIVAAACGYFSHADIDAAPAASFIWVKTFPLSVYGPMVLPIIAVFIICACECIGD Sbjct: 301 GLLVGCIVAAACGYFSHADIDAAPAASFIWVKTFPLSVYGPMVLPIIAVFIICACECIGD 360 Query: 361 VTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNNGVIALTRCAN 420 VTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNN VIALTRCAN Sbjct: 361 VTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNNVVIALTRCAN 420 Query: 421 RWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIVAKAPFTRRN 480 RWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIVAKAPFTRRN Sbjct: 421 RWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIVAKAPFTRRN 480 Query: 481 RFILTASMALGYGATLVPTWFGNVFPQTENRDLEGFENAIELVLETGFAVTAFVAMLLNA 540 RFILTASMALGYGATLVPTWFGNVFPQTENRDLEGFENAIELVLETGFAVTAFVAMLLNA Sbjct: 481 RFILTASMALGYGATLVPTWFGNVFPQTENRDLEGFENAIELVLETGFAVTAFVAMLLNA 540 Query: 541 IMPAEVEEIGAVTPMPVSAHDNRDGEAEYQSKQA 574 IMPAEVEEIGAVTPMPVSAHDNRDGEAEYQSKQA Sbjct: 541 IMPAEVEEIGAVTPMPVSAHDNRDGEAEYQSKQA 574 >3QE7-A nrPDB Length = 429 Score = 74.7 bits (182), Expect = 1e-13, Method: Compositional matrix adjust. Identities = 102/430 (23%), Positives = 182/430 (42%), Gaps = 78/430 (18%) Query: 69 GLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSLIVCGLLSM 128 G++E+ P+L L LQH AM V P++ + P+ T L+ G+ ++ Sbjct: 7 GVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHIN---PA--------TVLLFNGIGTL 55 Query: 129 VQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAGNRLPCPEA 188 + + K P Y+GS SF+ IS L P Sbjct: 56 LYLFICK-GKIPAYLGS--------SFAFISPV--------------------LLLLPLG 86 Query: 189 YGALIGTSACCALVEILLAFVPPKV----IQKIFPPIVTGPTVMLIGISLIGTGFKDWAG 244 Y +G C ++ L++F+ K + +FPP G V +IG+ L G A Sbjct: 87 YEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGV-----AA 141 Query: 245 GSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVIGLLV 304 G A G+L P S I + ++ + G+ + + +I +L+ Sbjct: 142 GMA----GLLPAEGQTPD-----SKTII-----ISITTLAVTVLGSVLFRGFLAIIPILI 187 Query: 305 GCIVAAACGYFSHADIDAAPAASFIWVKTFPL-SVYGPM-----VLPIIAVFIICACECI 358 G +V A F+ +D P + W F L ++Y P +L I+ ++ E + Sbjct: 188 GVLVGYALS-FAMGIVDTTPIINAHW---FALPTLYTPRFEWFAILTILPAALVVIAEHV 243 Query: 359 GDVTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNNGVIALTRC 418 G + T ++ + ++ + + ++ A+G+++V++ TP TT+ +N GV+A+TR Sbjct: 244 GHLVVTANIVKKDLLR---DPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRV 300 Query: 419 ANRWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIV--AKAPF 476 + W + I+ K AAAI IP VMGG+ L+ + SG ++ +K + Sbjct: 301 YSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDY 360 Query: 477 TRRNRFILTA 486 + ILT+ Sbjct: 361 NKAQNLILTS 370 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 5I6C-A nrPDB 949 0.0 3QE7-A nrPDB 385 e-107 Sequences not found previously or not previously below threshold: 2IZV-A nrPDB 30 3.5 3PPI-A nrPDB 29 6.3 CONVERGED! >5I6C-A nrPDB Length = 574 Score = 949 bits (2454), Expect = 0.0, Method: Composition-based stats. Identities = 573/574 (99%), Positives = 573/574 (99%) Query: 1 MDNSIHSTDGPDSVIPNSNPKKTVRQRVRLLARHLTTREGLIGDYDYGFLFRPELPFMKK 60 MDNSIHSTDGPDSVIPNSNPKKTVRQRVRLLARHLTTREGLIGDYDYGFLFRPELPFMKK Sbjct: 1 MDNSIHSTDGPDSVIPNSNPKKTVRQRVRLLARHLTTREGLIGDYDYGFLFRPELPFMKK 60 Query: 61 DPRAPPFFGLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSL 120 DPRAPPFFGLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSL Sbjct: 61 DPRAPPFFGLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSL 120 Query: 121 IVCGLLSMVQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAG 180 IVCGLLSMVQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAG Sbjct: 121 IVCGLLSMVQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAG 180 Query: 181 NRLPCPEAYGALIGTSACCALVEILLAFVPPKVIQKIFPPIVTGPTVMLIGISLIGTGFK 240 NRLPCPEAYGALIGTSACCALVEILLAFVPPKVIQKIFPPIVTGPTVMLIGISLIGTGFK Sbjct: 181 NRLPCPEAYGALIGTSACCALVEILLAFVPPKVIQKIFPPIVTGPTVMLIGISLIGTGFK 240 Query: 241 DWAGGSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVI 300 DWAGGSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVI Sbjct: 241 DWAGGSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVI 300 Query: 301 GLLVGCIVAAACGYFSHADIDAAPAASFIWVKTFPLSVYGPMVLPIIAVFIICACECIGD 360 GLLVGCIVAAACGYFSHADIDAAPAASFIWVKTFPLSVYGPMVLPIIAVFIICACECIGD Sbjct: 301 GLLVGCIVAAACGYFSHADIDAAPAASFIWVKTFPLSVYGPMVLPIIAVFIICACECIGD 360 Query: 361 VTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNNGVIALTRCAN 420 VTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNN VIALTRCAN Sbjct: 361 VTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNNVVIALTRCAN 420 Query: 421 RWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIVAKAPFTRRN 480 RWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIVAKAPFTRRN Sbjct: 421 RWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIVAKAPFTRRN 480 Query: 481 RFILTASMALGYGATLVPTWFGNVFPQTENRDLEGFENAIELVLETGFAVTAFVAMLLNA 540 RFILTASMALGYGATLVPTWFGNVFPQTENRDLEGFENAIELVLETGFAVTAFVAMLLNA Sbjct: 481 RFILTASMALGYGATLVPTWFGNVFPQTENRDLEGFENAIELVLETGFAVTAFVAMLLNA 540 Query: 541 IMPAEVEEIGAVTPMPVSAHDNRDGEAEYQSKQA 574 IMPAEVEEIGAVTPMPVSAHDNRDGEAEYQSKQA Sbjct: 541 IMPAEVEEIGAVTPMPVSAHDNRDGEAEYQSKQA 574 >3QE7-A nrPDB Length = 429 Score = 385 bits (990), Expect = e-107, Method: Composition-based stats. Identities = 101/430 (23%), Positives = 181/430 (42%), Gaps = 78/430 (18%) Query: 69 GLNEKIPVLLAFILGLQHALAMLAGVVTPPLIISSSLSLPSDLQQYLVSTSLIVCGLLSM 128 G++E+ P+L L LQH AM V P++ + +T L+ G+ ++ Sbjct: 7 GVSERPPLLQTIPLSLQHLFAMFGATVLVPVLFHIN-----------PATVLLFNGIGTL 55 Query: 129 VQITRFHIYKTPYYIGSGVLSVMGVSFSIISVASGAFNQMYSNGFCQLDEAGNRLPCPEA 188 + + K P Y+GS SF+ IS L P Sbjct: 56 LYLFICK-GKIPAYLGS--------SFAFISPV--------------------LLLLPLG 86 Query: 189 YGALIGTSACCALVEILLAFVPPKV----IQKIFPPIVTGPTVMLIGISLIGTGFKDWAG 244 Y +G C ++ L++F+ K + +FPP G V +IG+ L G A Sbjct: 87 YEVALGGFIMCGVLFCLVSFIVKKAGTGWLDVLFPPAAMGAIVAVIGLELAGV-----AA 141 Query: 245 GSACMDDGMLCPSATAPRPLPWGSPEFIGLGFLVFVSIILCERFGAPIMKSCSVVIGLLV 304 G A G+L P S I + ++ + G+ + + +I +L+ Sbjct: 142 GMA----GLLPAEGQTPD-----SKTII-----ISITTLAVTVLGSVLFRGFLAIIPILI 187 Query: 305 GCIVAAACGYFSHADIDAAPAASFIWVKTFPL-SVYGPM-----VLPIIAVFIICACECI 358 G +V A F+ +D P + W F L ++Y P +L I+ ++ E + Sbjct: 188 GVLVGYALS-FAMGIVDTTPIINAHW---FALPTLYTPRFEWFAILTILPAALVVIAEHV 243 Query: 359 GDVTATCDVSRLEVRGGTFESRIQGAVLADGINSVVAALATMTPMTTFAQNNGVIALTRC 418 G + T ++ + ++ + + ++ A+G+++V++ TP TT+ +N GV+A+TR Sbjct: 244 GHLVVTANIVKKDLLR---DPGLHRSMFANGLSTVISGFFGSTPNTTYGENIGVMAITRV 300 Query: 419 ANRWAGYCCCLILIVAGIFAKFAAAIVAIPNSVMGGMKTFLFASVVISGQAIV--AKAPF 476 + W + I+ K AAAI IP VMGG+ L+ + SG ++ +K + Sbjct: 301 YSTWVIGGAAIFAILLSCVGKLAAAIQMIPLPVMGGVSLLLYGVIGASGIRVLIESKVDY 360 Query: 477 TRRNRFILTA 486 + ILT+ Sbjct: 361 NKAQNLILTS 370 >2IZV-A nrPDB Length = 187 Score = 29.7 bits (65), Expect = 3.5, Method: Composition-based stats. Identities = 11/28 (39%), Positives = 13/28 (46%) Query: 60 KDPRAPPFFGLNEKIPVLLAFILGLQHA 87 KDP A FF P++ F LQH Sbjct: 115 KDPSACMFFEPLLSTPLIRTFPFSLQHI 142 >3PPI-A nrPDB Length = 281 Score = 28.9 bits (63), Expect = 6.3, Method: Composition-based stats. Identities = 19/64 (29%), Positives = 31/64 (48%), Gaps = 1/64 (1%) Query: 512 DLEGFENAIELVLETGFAVTAFVAMLLNAIMPAEVEEIGA-VTPMPVSAHDNRDGEAEYQ 570 D+ GF I+L L + V VA + A P E E GA V ++ ++ + G+ Y Sbjct: 129 DMGGFTKTIDLYLNGTYNVARLVAASIAAAEPRENGERGALVLTASIAGYEGQIGQTAYA 188 Query: 571 SKQA 574 + +A Sbjct: 189 AAKA 192 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.317 0.133 0.387 Lambda K H 0.267 0.0409 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 22,346,091 Number of Sequences: 36641 Number of extensions: 902755 Number of successful extensions: 2652 Number of sequences better than 10.0: 16 Number of HSP's better than 10.0 without gapping: 14 Number of HSP's successfully gapped in prelim test: 8 Number of HSP's that attempted gapping in prelim test: 2621 Number of HSP's gapped (non-prelim): 27 length of query: 574 length of database: 10,336,785 effective HSP length: 102 effective length of query: 472 effective length of database: 6,599,403 effective search space: 3114918216 effective search space used: 3114918216 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 40 (21.2 bits) S2: 62 (28.5 bits)