BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q06405 (101 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6CP5-U nrPDB 196 2e-51 5UR2-A nrPDB 28 0.98 2AD6-A nrPDB 27 1.5 6GCS-h nrPDB 27 1.6 4A5G-A nrPDB 27 1.8 3PZS-A nrPDB 26 3.2 2EMT-A nrPDB 25 9.8 >6CP5-U nrPDB Length = 95 Score = 196 bits (498), Expect = 2e-51, Method: Compositional matrix adjust. Identities = 95/95 (100%), Positives = 95/95 (100%) Query: 7 VSTLIPPKVVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDN 66 VSTLIPPKVVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDN Sbjct: 1 VSTLIPPKVVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDN 60 Query: 67 ASGKPLWHFALGIIAFGYSMEYYFHLRHHKGAEEH 101 ASGKPLWHFALGIIAFGYSMEYYFHLRHHKGAEEH Sbjct: 61 ASGKPLWHFALGIIAFGYSMEYYFHLRHHKGAEEH 95 >5UR2-A nrPDB Length = 984 Score = 28.1 bits (61), Expect = 0.98, Method: Compositional matrix adjust. Identities = 16/57 (28%), Positives = 27/57 (47%), Gaps = 5/57 (8%) Query: 23 SAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDNASGKPLWHFALGI 79 S N K N+ F LP +I + +AR+ +YF D + P+++ LG+ Sbjct: 43 SMKNEKFKTNMFRFVDVLP-----SINSGDEVARHLKEYFSEDGGTLPPVFNVGLGL 94 >2AD6-A nrPDB Length = 571 Score = 27.3 bits (59), Expect = 1.5, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 17/34 (50%) Query: 65 DNASGKPLWHFALGIIAFGYSMEYYFHLRHHKGA 98 DN GK LW+F + G M Y F + + G+ Sbjct: 491 DNKDGKELWNFKMPSGGIGSPMTYSFKGKQYIGS 524 >6GCS-h nrPDB Length = 138 Score = 27.3 bits (59), Expect = 1.6, Method: Compositional matrix adjust. Identities = 17/59 (28%), Positives = 25/59 (42%), Gaps = 2/59 (3%) Query: 20 NIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDNASGKPLWHFALG 78 +IG + + + ++ K I TR YK K D D + +P WHF LG Sbjct: 26 SIGDTKSGRLVGTDIYGNKFYETDHQDEIHLRTRYVEYKEK--DYDMSQVEPGWHFWLG 82 >4A5G-A nrPDB Length = 308 Score = 26.9 bits (58), Expect = 1.8, Method: Composition-based stats. Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 2/64 (3%) Query: 15 VVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDNASGKPLWH 74 ++S KN G N+ R NVV K+ + P + + T + ++ + SG P W Sbjct: 63 IISEKNAGPNANSARGFNVVDNIKTALENACPGVVSCTDVLALASQ--ASVSLSGGPSWT 120 Query: 75 FALG 78 LG Sbjct: 121 VDLG 124 >3PZS-A nrPDB Length = 289 Score = 26.2 bits (56), Expect = 3.2, Method: Compositional matrix adjust. Identities = 17/77 (22%), Positives = 32/77 (41%), Gaps = 2/77 (2%) Query: 8 STLIPPKVVSSKNIGSAPNAKRIANVVHFYKSL-PQGPAPAIKANTRLARYKAKYFDGDN 66 S +I P ++ + + S + + V +SL +GP + + A Y A F+ Sbjct: 142 SDMIAPNLLELEQL-SGERVENVEQAVQVARSLCARGPKVVLVKHLSRAGYHADCFEMLL 200 Query: 67 ASGKPLWHFALGIIAFG 83 + WH ++ FG Sbjct: 201 VTADDAWHICRPLVDFG 217 >2EMT-A nrPDB Length = 322 Score = 24.6 bits (52), Expect = 9.8, Method: Composition-based stats. Identities = 19/79 (24%), Positives = 34/79 (43%), Gaps = 5/79 (6%) Query: 10 LIPPKVVSSKNIGSAPNAKRIANVVHFYKSLPQGPA--PAIKANTRLARYKAKYFDGDNA 67 L+P +V+ + +RI N ++ + + + +K L Y YF+ N Sbjct: 154 LLPQRVLEQHKLTKEQWEERIQNWHEEHRGMLREDSMMEYLKIAQDLEMYGVNYFEIKNK 213 Query: 68 SGKPLWHFALGIIAFGYSM 86 G LW LG+ A G ++ Sbjct: 214 KGTELW---LGVDALGLNI 229 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6CP5-U nrPDB 188 4e-49 Sequences not found previously or not previously below threshold: 5UR2-A nrPDB 28 1.0 2AD6-A nrPDB 28 1.1 4A5G-A nrPDB 27 2.4 6GCS-h nrPDB 27 2.4 5NEW-A nrPDB 25 7.2 2Z0F-A nrPDB 25 7.9 CONVERGED! >6CP5-U nrPDB Length = 95 Score = 188 bits (478), Expect = 4e-49, Method: Composition-based stats. Identities = 95/95 (100%), Positives = 95/95 (100%) Query: 7 VSTLIPPKVVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDN 66 VSTLIPPKVVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDN Sbjct: 1 VSTLIPPKVVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDN 60 Query: 67 ASGKPLWHFALGIIAFGYSMEYYFHLRHHKGAEEH 101 ASGKPLWHFALGIIAFGYSMEYYFHLRHHKGAEEH Sbjct: 61 ASGKPLWHFALGIIAFGYSMEYYFHLRHHKGAEEH 95 >5UR2-A nrPDB Length = 984 Score = 28.1 bits (61), Expect = 1.0, Method: Composition-based stats. Identities = 16/57 (28%), Positives = 27/57 (47%), Gaps = 5/57 (8%) Query: 23 SAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDNASGKPLWHFALGI 79 S N K N+ F LP +I + +AR+ +YF D + P+++ LG+ Sbjct: 43 SMKNEKFKTNMFRFVDVLP-----SINSGDEVARHLKEYFSEDGGTLPPVFNVGLGL 94 >2AD6-A nrPDB Length = 571 Score = 27.7 bits (60), Expect = 1.1, Method: Composition-based stats. Identities = 12/34 (35%), Positives = 17/34 (50%) Query: 65 DNASGKPLWHFALGIIAFGYSMEYYFHLRHHKGA 98 DN GK LW+F + G M Y F + + G+ Sbjct: 491 DNKDGKELWNFKMPSGGIGSPMTYSFKGKQYIGS 524 >4A5G-A nrPDB Length = 308 Score = 26.5 bits (57), Expect = 2.4, Method: Composition-based stats. Identities = 18/64 (28%), Positives = 29/64 (45%), Gaps = 2/64 (3%) Query: 15 VVSSKNIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDNASGKPLWH 74 ++S KN G N+ R NVV K+ + P + + T + ++ + SG P W Sbjct: 63 IISEKNAGPNANSARGFNVVDNIKTALENACPGVVSCTDVLALASQ--ASVSLSGGPSWT 120 Query: 75 FALG 78 LG Sbjct: 121 VDLG 124 >6GCS-h nrPDB Length = 138 Score = 26.5 bits (57), Expect = 2.4, Method: Composition-based stats. Identities = 17/59 (28%), Positives = 25/59 (42%), Gaps = 2/59 (3%) Query: 20 NIGSAPNAKRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDNASGKPLWHFALG 78 +IG + + + ++ K I TR YK K D D + +P WHF LG Sbjct: 26 SIGDTKSGRLVGTDIYGNKFYETDHQDEIHLRTRYVEYKEK--DYDMSQVEPGWHFWLG 82 >5NEW-A nrPDB Length = 102 Score = 25.0 bits (53), Expect = 7.2, Method: Composition-based stats. Identities = 11/29 (37%), Positives = 19/29 (65%), Gaps = 2/29 (6%) Query: 1 MIFKRAVSTLIPPKVVS--SKNIGSAPNA 27 M++K A+ST++P + VS S N G ++ Sbjct: 53 MVYKHAISTVVPSRPVSHHSNNAGGGTSS 81 >2Z0F-A nrPDB Length = 524 Score = 25.0 bits (53), Expect = 7.9, Method: Composition-based stats. Identities = 17/57 (29%), Positives = 26/57 (45%), Gaps = 10/57 (17%) Query: 22 GSAPNA------KRIANVVHFYKSLPQGPAPAIKANTRLARYKAKYFDGDNASGKPL 72 G AP+A +++ + K LP P +A RLAR AK +G+P+ Sbjct: 406 GQAPDALYEALAEKLGRPYYARKDLPVSP----EAKARLARLSAKEVHPSTLAGEPV 458 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.321 0.136 0.419 Lambda K H 0.267 0.0418 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 4,566,126 Number of Sequences: 36641 Number of extensions: 169739 Number of successful extensions: 307 Number of sequences better than 10.0: 7 Number of HSP's better than 10.0 without gapping: 6 Number of HSP's successfully gapped in prelim test: 9 Number of HSP's that attempted gapping in prelim test: 301 Number of HSP's gapped (non-prelim): 15 length of query: 101 length of database: 10,336,785 effective HSP length: 68 effective length of query: 33 effective length of database: 7,845,197 effective search space: 258891501 effective search space used: 258891501 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 53 (25.0 bits)