BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q06263 (330 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6AP1-H nrPDB 669 0.0 1RZR-D nrPDB 31 0.96 3NUH-A nrPDB 29 3.1 1V5B-A nrPDB 28 5.2 5INW-A nrPDB 27 8.9 3OQN-A nrPDB 27 9.2 >6AP1-H nrPDB Length = 330 Score = 669 bits (1726), Expect = 0.0, Method: Compositional matrix adjust. Identities = 330/330 (100%), Positives = 330/330 (100%) Query: 1 MASNAARVVATAKDFDKVGLGIIGYYLQLYAVELILSEEDRSQEMTALATELLDTIEAFK 60 MASNAARVVATAKDFDKVGLGIIGYYLQLYAVELILSEEDRSQEMTALATELLDTIEAFK Sbjct: 1 MASNAARVVATAKDFDKVGLGIIGYYLQLYAVELILSEEDRSQEMTALATELLDTIEAFK 60 Query: 61 KEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQLKDGPWDVMLKRS 120 KEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQLKDGPWDVMLKRS Sbjct: 61 KEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQLKDGPWDVMLKRS 120 Query: 121 LWCCIDLFSCILHLWKENISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLDYADFA 180 LWCCIDLFSCILHLWKENISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLDYADFA Sbjct: 121 LWCCIDLFSCILHLWKENISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLDYADFA 180 Query: 181 DDSEEIKDEDVDHQTSDLENNNNDKVEGLAPKDQTTSYEPVDEVPEFIDDADSVNEEEQT 240 DDSEEIKDEDVDHQTSDLENNNNDKVEGLAPKDQTTSYEPVDEVPEFIDDADSVNEEEQT Sbjct: 181 DDSEEIKDEDVDHQTSDLENNNNDKVEGLAPKDQTTSYEPVDEVPEFIDDADSVNEEEQT 240 Query: 241 VDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAAAEHKSYTKDELTKIMDRASKIEQIQKL 300 VDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAAAEHKSYTKDELTKIMDRASKIEQIQKL Sbjct: 241 VDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAAAEHKSYTKDELTKIMDRASKIEQIQKL 300 Query: 301 AKYAISALNYEDLPTAKDELTKALDLLNSI 330 AKYAISALNYEDLPTAKDELTKALDLLNSI Sbjct: 301 AKYAISALNYEDLPTAKDELTKALDLLNSI 330 >1RZR-D nrPDB Length = 332 Score = 30.8 bits (68), Expect = 0.96, Method: Compositional matrix adjust. Identities = 20/74 (27%), Positives = 38/74 (51%), Gaps = 3/74 (4%) Query: 48 LATELLDTIEAFKKEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQ 107 LA + D +K I + ++ DK LH++N ++ Q I+M + ++ E +++ Sbjct: 78 LARGIEDIATMYKYNIILSNSDQNQDKELHLLNNMLGKQVDGIIFM---SGNVTEEHVEE 134 Query: 108 LKDGPWDVMLKRSL 121 LK P V+L S+ Sbjct: 135 LKKSPVPVVLAASI 148 >3NUH-A nrPDB Length = 525 Score = 28.9 bits (63), Expect = 3.1, Method: Compositional matrix adjust. Identities = 23/85 (27%), Positives = 43/85 (50%), Gaps = 5/85 (5%) Query: 151 IKYCKIYLSKLAKGEIGSSDEKTLDYADFADDSEEIKDEDVDHQTSDLENNNNDKVEGLA 210 ++Y +I L+K+A + +++T+D+ D D +E+I D + L N ++ G+A Sbjct: 120 MRYTEIRLAKIAHELMADLEKETVDFVDNYDGTEKIPDVMPTKIPNLLVNGSSGIAVGMA 179 Query: 211 ---PKDQTTSYEPVDEVPEFIDDAD 232 P T E ++ +IDD D Sbjct: 180 TNIPPHNLT--EVINGCLAYIDDED 202 >1V5B-A nrPDB Length = 366 Score = 28.1 bits (61), Expect = 5.2, Method: Compositional matrix adjust. Identities = 12/41 (29%), Positives = 25/41 (60%), Gaps = 3/41 (7%) Query: 262 LTRPS---APSEPAAAEHKSYTKDELTKIMDRASKIEQIQK 299 RPS P E + +YT++E+ +I+++A ++ QI++ Sbjct: 142 FGRPSERRGPGENEVVDTLAYTREEIERIIEKAFQLAQIRR 182 >5INW-A nrPDB Length = 421 Score = 27.3 bits (59), Expect = 8.9, Method: Compositional matrix adjust. Identities = 18/82 (21%), Positives = 31/82 (37%), Gaps = 2/82 (2%) Query: 82 LIHDQEKAKI--YMLNFTMSLYNEKLKQLKDGPWDVMLKRSLWCCIDLFSCILHLWKENI 139 +H ++KAK Y +LY G V K+ LW + HL+K N+ Sbjct: 141 FLHGKKKAKSMKYFARLNGALYKRSAGFELMGKNVVFSKKGLWLYRQFTRTVAHLFKSNV 200 Query: 140 SETSTNSLQKRIKYCKIYLSKL 161 + ++ Y+ K+ Sbjct: 201 RSVDFGDSKNAVELMNAYIEKV 222 >3OQN-A nrPDB Length = 339 Score = 27.3 bits (59), Expect = 9.2, Method: Compositional matrix adjust. Identities = 17/74 (22%), Positives = 37/74 (50%), Gaps = 3/74 (4%) Query: 48 LATELLDTIEAFKKEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQ 107 LA + D +K I + ++ +K LH++NT++ Q ++M ++ +E + + Sbjct: 78 LARGIEDIATMYKYNIILSNSDQNMEKELHLLNTMLGKQVDGIVFMGG---NITDEHVAE 134 Query: 108 LKDGPWDVMLKRSL 121 K P ++L S+ Sbjct: 135 FKRSPVPIVLAASV 148 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6AP1-H nrPDB 639 0.0 Sequences not found previously or not previously below threshold: 1RZR-D nrPDB 34 0.14 2XHE-B nrPDB 31 0.69 6FAI-k nrPDB 31 0.71 5WWN-A nrPDB 31 0.71 3RC8-A nrPDB 31 0.85 3EF1-A nrPDB 30 1.4 1V5B-A nrPDB 30 1.5 5GK3-A nrPDB 30 2.0 2I3E-A nrPDB 29 2.2 4PSP-A nrPDB 29 3.2 6ICZ-v nrPDB 28 5.4 6EEP-A nrPDB 28 5.8 5WLC-NA nrPDB 28 7.8 CONVERGED! >6AP1-H nrPDB Length = 330 Score = 639 bits (1647), Expect = 0.0, Method: Composition-based stats. Identities = 330/330 (100%), Positives = 330/330 (100%) Query: 1 MASNAARVVATAKDFDKVGLGIIGYYLQLYAVELILSEEDRSQEMTALATELLDTIEAFK 60 MASNAARVVATAKDFDKVGLGIIGYYLQLYAVELILSEEDRSQEMTALATELLDTIEAFK Sbjct: 1 MASNAARVVATAKDFDKVGLGIIGYYLQLYAVELILSEEDRSQEMTALATELLDTIEAFK 60 Query: 61 KEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQLKDGPWDVMLKRS 120 KEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQLKDGPWDVMLKRS Sbjct: 61 KEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQLKDGPWDVMLKRS 120 Query: 121 LWCCIDLFSCILHLWKENISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLDYADFA 180 LWCCIDLFSCILHLWKENISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLDYADFA Sbjct: 121 LWCCIDLFSCILHLWKENISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLDYADFA 180 Query: 181 DDSEEIKDEDVDHQTSDLENNNNDKVEGLAPKDQTTSYEPVDEVPEFIDDADSVNEEEQT 240 DDSEEIKDEDVDHQTSDLENNNNDKVEGLAPKDQTTSYEPVDEVPEFIDDADSVNEEEQT Sbjct: 181 DDSEEIKDEDVDHQTSDLENNNNDKVEGLAPKDQTTSYEPVDEVPEFIDDADSVNEEEQT 240 Query: 241 VDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAAAEHKSYTKDELTKIMDRASKIEQIQKL 300 VDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAAAEHKSYTKDELTKIMDRASKIEQIQKL Sbjct: 241 VDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAAAEHKSYTKDELTKIMDRASKIEQIQKL 300 Query: 301 AKYAISALNYEDLPTAKDELTKALDLLNSI 330 AKYAISALNYEDLPTAKDELTKALDLLNSI Sbjct: 301 AKYAISALNYEDLPTAKDELTKALDLLNSI 330 >1RZR-D nrPDB Length = 332 Score = 33.6 bits (75), Expect = 0.14, Method: Composition-based stats. Identities = 20/74 (27%), Positives = 38/74 (51%), Gaps = 3/74 (4%) Query: 48 LATELLDTIEAFKKEIGGESEAEDSDKSLHVMNTLIHDQEKAKIYMLNFTMSLYNEKLKQ 107 LA + D +K I + ++ DK LH++N ++ Q I+M + ++ E +++ Sbjct: 78 LARGIEDIATMYKYNIILSNSDQNQDKELHLLNNMLGKQVDGIIFM---SGNVTEEHVEE 134 Query: 108 LKDGPWDVMLKRSL 121 LK P V+L S+ Sbjct: 135 LKKSPVPVVLAASI 148 >2XHE-B nrPDB Length = 279 Score = 31.3 bits (69), Expect = 0.69, Method: Composition-based stats. Identities = 24/93 (25%), Positives = 42/93 (45%), Gaps = 4/93 (4%) Query: 232 DSVNEEEQTVDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAAAEHKSYTKDELTKIMDRA 291 D ++ Q +N+ A D + +++ T SA EP A+ + K I D Sbjct: 2 DRLSRLRQMAAENQPAEASDAAGGAEAQIEETSLSAQPEPFMADFFNRVKRIRDNIEDIE 61 Query: 292 SKIEQIQKLAKYAISALNYEDLPTAKDELTKAL 324 IEQ+ +L ++ A++ ED +D L + L Sbjct: 62 QAIEQVAQLHTESLVAVSKED----RDRLNEKL 90 >6FAI-k nrPDB Length = 788 Score = 30.9 bits (68), Expect = 0.71, Method: Composition-based stats. Identities = 32/131 (24%), Positives = 58/131 (44%), Gaps = 20/131 (15%) Query: 139 ISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLD-YADFADDSEEIKDEDVDHQTSD 197 I E +T+SL + ++ S + + TLD YA + +E+ D D D + Sbjct: 319 IKEKATDSLSLELDLQTVFESNM--------NRDTLDEYA--PEGTEDWSDYDEDFEYDG 368 Query: 198 LENNNNDKVEGLAPKDQTTSYEPVD------EVPEFIDD---ADSVNEEEQTVDKNEDAI 248 L D L ++QT+ V + ++DD A+ E EQT K+E + Sbjct: 369 LTTARYDDHGFLPGREQTSKKAAVPKGTSDYQAKWYLDDVIDANEEEEAEQTNGKDETMM 428 Query: 249 TKDEQQVVKKE 259 D++ +V+++ Sbjct: 429 EIDDEMMVEQD 439 >5WWN-A nrPDB Length = 709 Score = 30.9 bits (68), Expect = 0.71, Method: Composition-based stats. Identities = 32/131 (24%), Positives = 58/131 (44%), Gaps = 20/131 (15%) Query: 139 ISETSTNSLQKRIKYCKIYLSKLAKGEIGSSDEKTLD-YADFADDSEEIKDEDVDHQTSD 197 I E +T+SL + ++ S + + TLD YA + +E+ D D D + Sbjct: 240 IKEKATDSLSLELDLQTVFESNM--------NRDTLDEYA--PEGTEDWSDYDEDFEYDG 289 Query: 198 LENNNNDKVEGLAPKDQTTSYEPVD------EVPEFIDD---ADSVNEEEQTVDKNEDAI 248 L D L ++QT+ V + ++DD A+ E EQT K+E + Sbjct: 290 LTTARYDDHGFLPGREQTSKKAAVPKGTSDYQAKWYLDDVIDANEEEEAEQTNGKDETMM 349 Query: 249 TKDEQQVVKKE 259 D++ +V+++ Sbjct: 350 EIDDEMMVEQD 360 >3RC8-A nrPDB Length = 677 Score = 30.9 bits (68), Expect = 0.85, Method: Composition-based stats. Identities = 16/44 (36%), Positives = 26/44 (59%), Gaps = 1/44 (2%) Query: 265 PSAPSEPAAAEHKSYTKDELTKIMDRASKIEQIQKL-AKYAISA 307 PSA + A + K+E+ K++D+ K ++IQKL A Y + A Sbjct: 29 PSADGDVGAELTRPLDKNEVKKVLDKFYKRKEIQKLGADYGLDA 72 >3EF1-A nrPDB Length = 442 Score = 30.1 bits (66), Expect = 1.4, Method: Composition-based stats. Identities = 33/143 (23%), Positives = 61/143 (42%), Gaps = 29/143 (20%) Query: 197 DLENNNNDKVEGLAPKDQTTSYE-PVDEVPEFIDDADSVNEEEQTVDKNEDAITKDEQQV 255 D+ +N K L ++Q E P DE P+ +D+ + NEE D + + +D + Sbjct: 185 DINSNFLAKSTPLPEQEQLIPLEIPKDE-PDSVDEINEENEETPEYDSSNSSYAQDSSTI 243 Query: 256 VKK---------------EVDLTRPSA-----PSEPAAAEHKSYTKDE------LTKIMD 289 +K E + R +A P A + + +DE T + + Sbjct: 244 PEKTLLKDTFLQNREALEEQNKERVTALELQKSERPLAKQQNALLEDEGKPTPSHTLLHN 303 Query: 290 RASKIEQIQKLAKYAISALNYED 312 R ++E+++K+ K I A+ YE+ Sbjct: 304 RDHELERLEKVLK-DIHAVYYEE 325 >1V5B-A nrPDB Length = 366 Score = 30.1 bits (66), Expect = 1.5, Method: Composition-based stats. Identities = 12/39 (30%), Positives = 25/39 (64%), Gaps = 3/39 (7%) Query: 264 RPS---APSEPAAAEHKSYTKDELTKIMDRASKIEQIQK 299 RPS P E + +YT++E+ +I+++A ++ QI++ Sbjct: 144 RPSERRGPGENEVVDTLAYTREEIERIIEKAFQLAQIRR 182 >5GK3-A nrPDB Length = 359 Score = 29.7 bits (65), Expect = 2.0, Method: Composition-based stats. Identities = 19/73 (26%), Positives = 38/73 (52%), Gaps = 8/73 (10%) Query: 134 LWKENISETSTNSLQKRIKYCKIYLSKLAK------GEIGSS--DEKTLDYADFADDSEE 185 L+ ++ + S SLQ+ I+ C YL +++K E+G + +E +D + + Sbjct: 138 LFSSHMIDLSEESLQENIEICSKYLERMSKIGMTLEIELGCTGGEEDGVDNSHMDASALY 197 Query: 186 IKDEDVDHQTSDL 198 + EDVD+ ++L Sbjct: 198 TQPEDVDYAYTEL 210 >2I3E-A nrPDB Length = 222 Score = 29.3 bits (64), Expect = 2.2, Method: Composition-based stats. Identities = 17/40 (42%), Positives = 24/40 (60%), Gaps = 3/40 (7%) Query: 34 LILSEEDRSQEMTALATELLDTIEAFKKEIG---GESEAE 70 L+ EE+R + T + LDT+EAFK+ I GE+E E Sbjct: 14 LLPEEEERIKCATMDFLKTLDTLEAFKEHISEFTGEAEKE 53 >4PSP-A nrPDB Length = 585 Score = 28.9 bits (63), Expect = 3.2, Method: Composition-based stats. Identities = 15/44 (34%), Positives = 22/44 (50%) Query: 230 DADSVNEEEQTVDKNEDAITKDEQQVVKKEVDLTRPSAPSEPAA 273 D +S+ E DK D + E + K +D+T PS P+E A Sbjct: 441 DLESLKSVELLGDKAGDYVKVSEWEQSKDALDITLPSQPAESLA 484 >6ICZ-v nrPDB Length = 148 Score = 28.2 bits (61), Expect = 5.4, Method: Composition-based stats. Identities = 19/90 (21%), Positives = 42/90 (46%), Gaps = 16/90 (17%) Query: 200 NNNNDKVEGLAPKDQTTSYEPVDEVPEFIDDADSVNEEEQTVDKNEDAITKDEQQVVKKE 259 NN+N K + + K+ ++E+ IDD++ + K +DA+ +V ++E Sbjct: 38 NNSNYKNDVMIRKEAYVHKSVMEELKRIIDDSE--------ITKEDDALWPPPDRVGRQE 89 Query: 260 VDLTRPSAPSEPAAAEHKSYTKDELTKIMD 289 +++ EH S+T ++ ++D Sbjct: 90 LEIV--------IGDEHISFTTSKIGSLID 111 >6EEP-A nrPDB Length = 218 Score = 28.2 bits (61), Expect = 5.8, Method: Composition-based stats. Identities = 15/53 (28%), Positives = 25/53 (47%), Gaps = 15/53 (28%) Query: 221 VDEVPEFIDDADSVNEEEQTVDKNEDAITK---------------DEQQVVKK 258 V ++P ++D AD VNE + + A+T+ DE +VVK+ Sbjct: 73 VQDLPIYVDGADEVNERGEMIKGGGGALTREKIVANVATQFICIVDESKVVKR 125 >5WLC-NA nrPDB Length = 593 Score = 27.8 bits (60), Expect = 7.8, Method: Composition-based stats. Identities = 24/70 (34%), Positives = 34/70 (48%), Gaps = 5/70 (7%) Query: 197 DLENNNNDKVEGLAPKDQTTSYE-PVDEVPEFIDDADSVNEEEQTVDKNEDAITKDEQQV 255 DLE N D LA +D + E DE ++ D S +EEE+ + ED K ++ Sbjct: 189 DLEKFNRDT---LAAEDSNEASEGSEDEDIDYFQDMPSDDEEEEAI-YYEDFFDKPTKEP 244 Query: 256 VKKEVDLTRP 265 VKK D+ P Sbjct: 245 VKKHSDVKDP 254 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.310 0.128 0.348 Lambda K H 0.267 0.0400 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 12,317,076 Number of Sequences: 36641 Number of extensions: 502905 Number of successful extensions: 1516 Number of sequences better than 10.0: 33 Number of HSP's better than 10.0 without gapping: 28 Number of HSP's successfully gapped in prelim test: 61 Number of HSP's that attempted gapping in prelim test: 1448 Number of HSP's gapped (non-prelim): 116 length of query: 330 length of database: 10,336,785 effective HSP length: 97 effective length of query: 233 effective length of database: 6,782,608 effective search space: 1580347664 effective search space used: 1580347664 T: 11 A: 40 X1: 16 ( 7.1 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.7 bits) S2: 59 (27.4 bits)