BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q06208 (395 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 4BJ6-A nrPDB 733 0.0 2CE7-A nrPDB 31 0.77 6N3Q-F nrPDB 29 4.4 3CO4-A nrPDB 28 6.6 3ZXU-B nrPDB 28 7.7 >4BJ6-A nrPDB Length = 365 Score = 733 bits (1893), Expect = 0.0, Method: Compositional matrix adjust. Identities = 361/362 (99%), Positives = 362/362 (100%) Query: 34 KNFTVLRKLNLVPIKKSVSSPKVCKPSPVKERVDHVFYQKFKSMALQELGTNYLSISYVP 93 +NFTVLRKLNLVPIKKSVSSPKVCKPSPVKERVDHVFYQKFKSMALQELGTNYLSISYVP Sbjct: 4 RNFTVLRKLNLVPIKKSVSSPKVCKPSPVKERVDHVFYQKFKSMALQELGTNYLSISYVP 63 Query: 94 SLSKFLSKNLRSMKNCIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEG 153 SLSKFLSKNLRSMKNCIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEG Sbjct: 64 SLSKFLSKNLRSMKNCIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEG 123 Query: 154 IQSSKSKECIESMGQASYSGQLDFEASEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFE 213 IQSSKSKECIESMGQASYSGQLDFEASEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFE Sbjct: 124 IQSSKSKECIESMGQASYSGQLDFEASEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFE 183 Query: 214 QLDKLSTSTIIEPSKLTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTK 273 QLDKLSTSTIIEPSKLTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTK Sbjct: 184 QLDKLSTSTIIEPSKLTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTK 243 Query: 274 YTVFEMPILTCAQEQEYLKKMIKFTFDSGSKLLQSYNSLVTCQLNNKESNLAIFFEFLKV 333 YTVFEMPILTCAQEQEYLKKMIKFTFDSGSKLLQSYNSLVTCQLNNKESNLAIFFEFLKV Sbjct: 244 YTVFEMPILTCAQEQEYLKKMIKFTFDSGSKLLQSYNSLVTCQLNNKESNLAIFFEFLKV 303 Query: 334 FPHPFTYLFNAYTEIIVQSRTFDELLDKIRNRLTIKNYPHSAYNFKKNQRLPLKLTRKVH 393 FPHPFTYLFNAYTEIIVQSRTFDELLDKIRNRLTIKNYPHSAYNFKKNQRLPLKLTRKVH Sbjct: 304 FPHPFTYLFNAYTEIIVQSRTFDELLDKIRNRLTIKNYPHSAYNFKKNQRLPLKLTRKVH 363 Query: 394 DR 395 DR Sbjct: 364 DR 365 >2CE7-A nrPDB Length = 476 Score = 31.2 bits (69), Expect = 0.77, Method: Compositional matrix adjust. Identities = 13/46 (28%), Positives = 25/46 (54%) Query: 109 CIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEGI 154 CIVF D+++ + ++ G +N +++EM+ + KEGI Sbjct: 110 CIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDSKEGI 155 >6N3Q-F nrPDB Length = 193 Score = 28.9 bits (63), Expect = 4.4, Method: Compositional matrix adjust. Identities = 27/93 (29%), Positives = 40/93 (43%), Gaps = 15/93 (16%) Query: 246 AFKVLIYSNNVSISSLLSTSLKKKLNTKYTVFEMPILTCAQEQEYLKKMIKFTFDSGSKL 305 A L N+ ++L+TSL + N +T Q E L KMIK F+SG K Sbjct: 18 AVVALSTETNIDQINVLTTSLIGETNPNFT---------PQPNEALSKMIKGLFESGMKN 68 Query: 306 LQSYNSLVTCQLNNKESNLAIFFEFLKVFPHPF 338 LQ +LN N+++ E + P+ Sbjct: 69 LQQK------KLNEALKNVSLAIEMAQRKRAPW 95 >3CO4-A nrPDB Length = 312 Score = 28.1 bits (61), Expect = 6.6, Method: Compositional matrix adjust. Identities = 16/65 (24%), Positives = 33/65 (50%) Query: 299 FDSGSKLLQSYNSLVTCQLNNKESNLAIFFEFLKVFPHPFTYLFNAYTEIIVQSRTFDEL 358 F G L + N L+TC +N++ N +E + + +Y A+T+ VQ ++D+ Sbjct: 129 FARGLYLAKEKNMLMTCAVNSRWLNYGTEWEQYFDYINLMSYDRGAFTDKPVQHASYDDF 188 Query: 359 LDKIR 363 + ++ Sbjct: 189 VKDLK 193 >3ZXU-B nrPDB Length = 270 Score = 28.1 bits (61), Expect = 7.7, Method: Compositional matrix adjust. Identities = 20/67 (29%), Positives = 36/67 (53%), Gaps = 6/67 (8%) Query: 166 MGQASYSGQLDFEA------SEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFEQLDKLS 219 M S SG LD E S++PS+H+ + ++ I E ++++ + E LD+LS Sbjct: 1 MDFTSSSGVLDSERNTGSNDSDEPSSHSDVIETEELKLIKLQEHKNNLLRQRSELLDQLS 60 Query: 220 TSTIIEP 226 + ++EP Sbjct: 61 QTRVVEP 67 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 4BJ6-A nrPDB 670 0.0 Sequences not found previously or not previously below threshold: 1M9N-A nrPDB 32 0.56 6BWT-A nrPDB 30 1.5 6N3Q-F nrPDB 30 2.1 3HOX-E nrPDB 29 2.9 3VZI-A nrPDB 29 3.5 3ZXU-B nrPDB 29 3.8 2CE7-A nrPDB 28 5.0 4V06-A nrPDB 28 6.9 1YGP-A nrPDB 28 8.7 CONVERGED! >4BJ6-A nrPDB Length = 365 Score = 670 bits (1728), Expect = 0.0, Method: Composition-based stats. Identities = 361/362 (99%), Positives = 362/362 (100%) Query: 34 KNFTVLRKLNLVPIKKSVSSPKVCKPSPVKERVDHVFYQKFKSMALQELGTNYLSISYVP 93 +NFTVLRKLNLVPIKKSVSSPKVCKPSPVKERVDHVFYQKFKSMALQELGTNYLSISYVP Sbjct: 4 RNFTVLRKLNLVPIKKSVSSPKVCKPSPVKERVDHVFYQKFKSMALQELGTNYLSISYVP 63 Query: 94 SLSKFLSKNLRSMKNCIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEG 153 SLSKFLSKNLRSMKNCIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEG Sbjct: 64 SLSKFLSKNLRSMKNCIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEG 123 Query: 154 IQSSKSKECIESMGQASYSGQLDFEASEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFE 213 IQSSKSKECIESMGQASYSGQLDFEASEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFE Sbjct: 124 IQSSKSKECIESMGQASYSGQLDFEASEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFE 183 Query: 214 QLDKLSTSTIIEPSKLTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTK 273 QLDKLSTSTIIEPSKLTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTK Sbjct: 184 QLDKLSTSTIIEPSKLTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTK 243 Query: 274 YTVFEMPILTCAQEQEYLKKMIKFTFDSGSKLLQSYNSLVTCQLNNKESNLAIFFEFLKV 333 YTVFEMPILTCAQEQEYLKKMIKFTFDSGSKLLQSYNSLVTCQLNNKESNLAIFFEFLKV Sbjct: 244 YTVFEMPILTCAQEQEYLKKMIKFTFDSGSKLLQSYNSLVTCQLNNKESNLAIFFEFLKV 303 Query: 334 FPHPFTYLFNAYTEIIVQSRTFDELLDKIRNRLTIKNYPHSAYNFKKNQRLPLKLTRKVH 393 FPHPFTYLFNAYTEIIVQSRTFDELLDKIRNRLTIKNYPHSAYNFKKNQRLPLKLTRKVH Sbjct: 304 FPHPFTYLFNAYTEIIVQSRTFDELLDKIRNRLTIKNYPHSAYNFKKNQRLPLKLTRKVH 363 Query: 394 DR 395 DR Sbjct: 364 DR 365 >1M9N-A nrPDB Length = 613 Score = 31.9 bits (71), Expect = 0.56, Method: Composition-based stats. Identities = 16/40 (40%), Positives = 27/40 (67%), Gaps = 3/40 (7%) Query: 30 DLEQKNFTVLRKL--NLVPIKKSVSSPKVCKPSPVKERVD 67 D+ +++F+++R + NL P K+VSSP V P V E++D Sbjct: 108 DMNKQDFSLVRVVVCNLYPFVKTVSSPGVTVPEAV-EKID 146 >6BWT-A nrPDB Length = 319 Score = 30.4 bits (67), Expect = 1.5, Method: Composition-based stats. Identities = 23/70 (32%), Positives = 32/70 (45%), Gaps = 2/70 (2%) Query: 94 SLSKFLSKNLRSMKNCIVFFDKVEHIHQYAGIDRAVSETLSLVDI--NVVIIEMNDYLMK 151 S KF+ K + + C FF K + + G + AV E L L +I +V +I D L Sbjct: 125 SEEKFMGKGVSACATCDGFFYKNKDVAVVGGGNTAVEEALFLSNIAKSVTLIHRRDTLRS 184 Query: 152 EGIQSSKSKE 161 E I K E Sbjct: 185 EKILIDKLME 194 >6N3Q-F nrPDB Length = 193 Score = 30.0 bits (66), Expect = 2.1, Method: Composition-based stats. Identities = 26/84 (30%), Positives = 37/84 (44%), Gaps = 15/84 (17%) Query: 246 AFKVLIYSNNVSISSLLSTSLKKKLNTKYTVFEMPILTCAQEQEYLKKMIKFTFDSGSKL 305 A L N+ ++L+TSL + N +T Q E L KMIK F+SG K Sbjct: 18 AVVALSTETNIDQINVLTTSLIGETNPNFT---------PQPNEALSKMIKGLFESGMKN 68 Query: 306 LQSYNSLVTCQLNNKESNLAIFFE 329 LQ +LN N+++ E Sbjct: 69 LQQK------KLNEALKNVSLAIE 86 >3HOX-E nrPDB Length = 215 Score = 29.2 bits (64), Expect = 2.9, Method: Composition-based stats. Identities = 32/135 (23%), Positives = 54/135 (40%), Gaps = 8/135 (5%) Query: 176 DFEASEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFEQLDKLSTSTIIEPSKLTEFINV 235 DF+A S MM + +ESI KF + L EPS + + Sbjct: 41 DFKAKYCDSMGRPQRKMMSFQANPTEESIS-----KFPDMGSLWVEFCDEPSVGVKTMKT 95 Query: 236 LSVLEKSNNIAFKVLIYSNNVSISSL-LSTSLKKKLNTKYTVFEMPILTCAQEQEYLKKM 294 + + N + +Y NN++ S++ L S+ T T E ++ E + K Sbjct: 96 FVIHIQEKNFQTGIFVYQNNITPSAMKLVPSIPPA--TIETFNEAALVVNITHHELVPKH 153 Query: 295 IKFTFDSGSKLLQSY 309 I+ + D +LL+ Y Sbjct: 154 IRLSSDEKRELLKRY 168 >3VZI-A nrPDB Length = 226 Score = 29.2 bits (64), Expect = 3.5, Method: Composition-based stats. Identities = 12/37 (32%), Positives = 24/37 (64%) Query: 9 APIRRSKKVVDSDKIVKAISDDLEQKNFTVLRKLNLV 45 A I ++ K +D++VK + +D +Q+ TVLR++ + Sbjct: 81 ASISKAIKAGRTDELVKYVEEDRQQRAATVLREVGYI 117 >3ZXU-B nrPDB Length = 270 Score = 28.8 bits (63), Expect = 3.8, Method: Composition-based stats. Identities = 35/136 (25%), Positives = 64/136 (47%), Gaps = 9/136 (6%) Query: 166 MGQASYSGQLDFEA------SEKPSNHTSDLMMMVMRKINNDESIDHIVYFKFEQLDKLS 219 M S SG LD E S++PS+H+ + ++ I E ++++ + E LD+LS Sbjct: 1 MDFTSSSGVLDSERNTGSNDSDEPSSHSDVIETEELKLIKLQEHKNNLLRQRSELLDQLS 60 Query: 220 TSTIIEPSKLTEFINVLSVLEKSNNIAFKVLIYSNNVSISSLLSTSLKKKLNTKYTVFEM 279 + ++EP + +L L + N+ A S+N + +L SL + KY + Sbjct: 61 QTRVVEPRSVQLDDKLLLKLLRRNDNAVSDSSQSSNNPLPRVLP-SLNIEQRKKYLDITL 119 Query: 280 P--ILTCAQEQEYLKK 293 +TC ++ L+K Sbjct: 120 NDVTVTCEKDMILLRK 135 >2CE7-A nrPDB Length = 476 Score = 28.4 bits (62), Expect = 5.0, Method: Composition-based stats. Identities = 13/46 (28%), Positives = 25/46 (54%) Query: 109 CIVFFDKVEHIHQYAGIDRAVSETLSLVDINVVIIEMNDYLMKEGI 154 CIVF D+++ + ++ G +N +++EM+ + KEGI Sbjct: 110 CIVFIDEIDAVGRHRGAGLGGGHDEREQTLNQLLVEMDGFDSKEGI 155 >4V06-A nrPDB Length = 349 Score = 28.0 bits (61), Expect = 6.9, Method: Composition-based stats. Identities = 13/41 (31%), Positives = 23/41 (56%), Gaps = 3/41 (7%) Query: 329 EFLKVFPHPFTYLFNAYT---EIIVQSRTFDELLDKIRNRL 366 +F K PF+ FN YT EI+ +R+ + ++ +R+ L Sbjct: 293 DFAKSITRPFSVYFNPYTQSIEILKDTRSIENVVQDLRSDL 333 >1YGP-A nrPDB Length = 879 Score = 27.7 bits (60), Expect = 8.7, Method: Composition-based stats. Identities = 16/38 (42%), Positives = 24/38 (63%), Gaps = 1/38 (2%) Query: 198 INNDESIDHIVYFKFEQLDKLSTSTIIEP-SKLTEFIN 234 +NNDESI+H++ F +S + II P S L+E I+ Sbjct: 678 VNNDESIEHLLKVVFVADYNVSKAEIIIPASDLSEHIS 715 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.315 0.139 0.404 Lambda K H 0.267 0.0429 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 15,885,221 Number of Sequences: 36641 Number of extensions: 673336 Number of successful extensions: 1739 Number of sequences better than 10.0: 14 Number of HSP's better than 10.0 without gapping: 7 Number of HSP's successfully gapped in prelim test: 20 Number of HSP's that attempted gapping in prelim test: 1724 Number of HSP's gapped (non-prelim): 31 length of query: 395 length of database: 10,336,785 effective HSP length: 99 effective length of query: 296 effective length of database: 6,709,326 effective search space: 1985960496 effective search space used: 1985960496 T: 11 A: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.5 bits) S2: 60 (27.7 bits)