BLASTP 2.2.23 [Feb-03-2010] Reference: Altschul, Stephen F., Thomas L. Madden, Alejandro A. Schaffer, Jinghui Zhang, Zheng Zhang, Webb Miller, and David J. Lipman (1997), "Gapped BLAST and PSI-BLAST: a new generation of protein database search programs", Nucleic Acids Res. 25:3389-3402. Reference for compositional score matrix adjustment: Altschul, Stephen F., John C. Wootton, E. Michael Gertz, Richa Agarwala, Aleksandr Morgulis, Alejandro A. Schaffer, and Yi-Kuo Yu (2005) "Protein database searches using compositionally adjusted substitution matrices", FEBS J. 272:5101-5109. Reference for composition-based statistics starting in round 2: Schaffer, Alejandro A., L. Aravind, Thomas L. Madden, Sergei Shavirin, John L. Spouge, Yuri I. Wolf, Eugene V. Koonin, and Stephen F. Altschul (2001), "Improving the accuracy of PSI-BLAST protein database searches with composition-based statistics and other refinements", Nucleic Acids Res. 29:2994-3005. Query= Q06102 (208 letters) Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta 36,641 sequences; 10,336,785 total letters Searching..................................................done Results from round 1 Score E Sequences producing significant alignments: (bits) Value 6EOJ-B nrPDB 426 e-120 6DNH-C nrPDB 137 2e-33 6ID0-O nrPDB 30 0.69 6QDV-M nrPDB 30 0.94 1D2E-A nrPDB 29 1.5 2O1B-A nrPDB 29 1.7 4YH8-A nrPDB 29 1.8 3JB9-Y nrPDB 28 2.0 3UJ2-A nrPDB 28 2.2 4N0B-A nrPDB 28 3.3 5LJ5-M nrPDB 27 6.6 3U1M-A nrPDB 27 8.4 >6EOJ-B nrPDB Length = 208 Score = 426 bits (1095), Expect = e-120, Method: Compositional matrix adjust. Identities = 207/207 (100%), Positives = 207/207 (100%) Query: 2 SLIHPDTAKYPFKFEPFLRQEYSFSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIF 61 SLIHPDTAKYPFKFEPFLRQEYSFSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIF Sbjct: 2 SLIHPDTAKYPFKFEPFLRQEYSFSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIF 61 Query: 62 QNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLHIDPASKI 121 QNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLHIDPASKI Sbjct: 62 QNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLHIDPASKI 121 Query: 122 PKCENYEMGFCPLGSSCPRRHIKKVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRI 181 PKCENYEMGFCPLGSSCPRRHIKKVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRI Sbjct: 122 PKCENYEMGFCPLGSSCPRRHIKKVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRI 181 Query: 182 KRDDEINTRKMDEEKERRLNAIINGEV 208 KRDDEINTRKMDEEKERRLNAIINGEV Sbjct: 182 KRDDEINTRKMDEEKERRLNAIINGEV 208 >6DNH-C nrPDB Length = 245 Score = 137 bits (346), Expect = 2e-33, Method: Compositional matrix adjust. Identities = 68/141 (48%), Positives = 91/141 (64%), Gaps = 8/141 (5%) Query: 33 ICEFYNSREGPKSCPRGPLCPKKHVLPIFQNKIVCRHWLRGLCKKNDQCEYLHEYNLRKM 92 +CEF+ +C +G +CP +H+ + +VC+HWLRGLCKK DQCE+LHEY++ KM Sbjct: 41 VCEFFLK----AACGKGGMCPFRHISG--EKTVVCKHWLRGLCKKGDQCEFLHEYDMTKM 94 Query: 93 PECVFFSKNGYCTQSPDCQYLHIDPASKIPKCENYEMGFCPLGSSCPRRHIKKVFCQRYM 152 PEC F+SK G C+ +C +LHIDP SKI C Y+ GFC G C RH ++V C Y+ Sbjct: 95 PECYFYSKFGECSNK-ECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 153 Query: 153 TGFCPLGKDECDMEHPQFIIP 173 GFCP G C HP+F +P Sbjct: 154 VGFCPEGPS-CKFMHPRFELP 173 >6ID0-O nrPDB Length = 420 Score = 30.0 bits (66), Expect = 0.69, Method: Compositional matrix adjust. Identities = 9/21 (42%), Positives = 15/21 (71%) Query: 66 VCRHWLRGLCKKNDQCEYLHE 86 +C W++G CK+ ++C Y HE Sbjct: 164 ICSFWVKGECKRGEECPYRHE 184 >6QDV-M nrPDB Length = 289 Score = 29.6 bits (65), Expect = 0.94, Method: Compositional matrix adjust. Identities = 9/21 (42%), Positives = 15/21 (71%) Query: 66 VCRHWLRGLCKKNDQCEYLHE 86 +C W++G CK+ ++C Y HE Sbjct: 147 ICSFWVKGECKRGEECPYRHE 167 >1D2E-A nrPDB Length = 397 Score = 28.9 bits (63), Expect = 1.5, Method: Compositional matrix adjust. Identities = 16/44 (36%), Positives = 24/44 (54%), Gaps = 2/44 (4%) Query: 51 LCPKKHVLPI-FQNKIVCRHWLRGLCKKNDQCEYL-HEYNLRKM 92 L P + V I + +V RG+ KK D+CE+L H N+R + Sbjct: 205 LLPVESVYSIPGRGTVVTGTLERGILKKGDECEFLGHSKNIRTV 248 >2O1B-A nrPDB Length = 404 Score = 28.9 bits (63), Expect = 1.7, Method: Compositional matrix adjust. Identities = 11/41 (26%), Positives = 23/41 (56%) Query: 18 FLRQEYSFSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVL 58 F +++Y+ +LD + +C Y ++ G + P + P +VL Sbjct: 97 FYQRQYNVTLDKEDEVCILYGTKNGLVAVPTCVINPGDYVL 137 >4YH8-A nrPDB Length = 216 Score = 28.9 bits (63), Expect = 1.8, Method: Compositional matrix adjust. Identities = 14/43 (32%), Positives = 20/43 (46%), Gaps = 3/43 (6%) Query: 25 FSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIFQNKIVC 67 + + D+ C FY +C G C +KHV P F I+C Sbjct: 9 YGTEQDKVNCSFYYK---IGACRHGERCSRKHVKPNFSQTILC 48 Score = 27.3 bits (59), Expect = 4.8, Method: Compositional matrix adjust. Identities = 13/31 (41%), Positives = 17/31 (54%), Gaps = 3/31 (9%) Query: 127 YEMGFCPLGSSCPRRHIKKVFCQRYMTGFCP 157 Y++G C G C R+H+K F Q T CP Sbjct: 22 YKIGACRHGERCSRKHVKPNFSQ---TILCP 49 >3JB9-Y nrPDB Length = 388 Score = 28.5 bits (62), Expect = 2.0, Method: Compositional matrix adjust. Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 6/39 (15%) Query: 67 CRHWLRGLCKKNDQCEYLHEYNLRKMP-ECVFFSKNGYC 104 C ++ RG+C + +CEYLH ++P + FF+ N C Sbjct: 117 CLYFARGMCSEGSKCEYLH-----RLPKDTDFFNANVDC 150 >3UJ2-A nrPDB Length = 449 Score = 28.5 bits (62), Expect = 2.2, Method: Compositional matrix adjust. Identities = 21/63 (33%), Positives = 34/63 (53%), Gaps = 3/63 (4%) Query: 145 KVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRIKRDD--EINTRKMDEE-KERRLN 201 K C+RY G DE D E Q++ + G K+++ DD NT ++++ KER N Sbjct: 299 KSLCERYPIVSIEDGLDEEDWEGWQYMTRELGDKIQLVGDDLFVTNTERLNKGIKERCGN 358 Query: 202 AII 204 +I+ Sbjct: 359 SIL 361 >4N0B-A nrPDB Length = 479 Score = 27.7 bits (60), Expect = 3.3, Method: Compositional matrix adjust. Identities = 27/131 (20%), Positives = 59/131 (45%), Gaps = 29/131 (22%) Query: 77 KNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLHIDPASKIPK----CENYEMGFC 132 +++Q +Y+++ +K+ + + S+N + P SK+P EN ++ Sbjct: 9 RSEQADYIYQQIYQKLKKEIL-SRN-------------LLPHSKVPSKRELAENLKVSVN 54 Query: 133 PLGSSCPRRHIKKVFCQRYMTGFCPLG--KDECDM----EHPQFIIPDEGSKLRIKRDDE 186 + S+ +++ + Y+ G +E DM EHP F +PD+ ++ I + D Sbjct: 55 SVNSAY-----QQLLAEGYLYAIERKGFFVEELDMFSAEEHPPFALPDDLKEIHIDQSDW 109 Query: 187 INTRKMDEEKE 197 I+ M + + Sbjct: 110 ISFSHMSSDTD 120 >5LJ5-M nrPDB Length = 339 Score = 26.9 bits (58), Expect = 6.6, Method: Compositional matrix adjust. Identities = 11/20 (55%), Positives = 14/20 (70%), Gaps = 1/20 (5%) Query: 95 CVFFSKNGYCTQSPDCQYLH 114 C+FF+K G C P C+YLH Sbjct: 73 CLFFAK-GMCCLGPKCEYLH 91 >3U1M-A nrPDB Length = 240 Score = 26.6 bits (57), Expect = 8.4, Method: Compositional matrix adjust. Identities = 12/23 (52%), Positives = 15/23 (65%), Gaps = 1/23 (4%) Query: 95 CVFFSKNGYCTQSPDCQYLHIDP 117 C+FF+K G C P C+YLH P Sbjct: 73 CLFFAK-GMCCLGPKCEYLHHIP 94 Searching..................................................done Results from round 2 Score E Sequences producing significant alignments: (bits) Value Sequences used in model and found again: 6EOJ-B nrPDB 363 e-101 6DNH-C nrPDB 230 4e-61 Sequences not found previously or not previously below threshold: 6G38-A nrPDB 33 0.071 4YH8-A nrPDB 33 0.094 6ID0-O nrPDB 32 0.19 6QDV-M nrPDB 32 0.23 4CS7-A nrPDB 32 0.23 3JB9-Y nrPDB 32 0.29 1D2E-A nrPDB 29 1.3 2D9M-A nrPDB 29 1.3 5LJ5-M nrPDB 28 4.1 4C3B-A nrPDB 27 5.1 3U1M-A nrPDB 27 5.4 3UJ2-A nrPDB 27 6.1 1ZTD-A nrPDB 27 6.7 5WDX-A nrPDB 26 8.2 4N0B-A nrPDB 26 8.6 CONVERGED! >6EOJ-B nrPDB Length = 208 Score = 363 bits (932), Expect = e-101, Method: Composition-based stats. Identities = 207/207 (100%), Positives = 207/207 (100%) Query: 2 SLIHPDTAKYPFKFEPFLRQEYSFSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIF 61 SLIHPDTAKYPFKFEPFLRQEYSFSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIF Sbjct: 2 SLIHPDTAKYPFKFEPFLRQEYSFSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIF 61 Query: 62 QNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLHIDPASKI 121 QNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLHIDPASKI Sbjct: 62 QNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLHIDPASKI 121 Query: 122 PKCENYEMGFCPLGSSCPRRHIKKVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRI 181 PKCENYEMGFCPLGSSCPRRHIKKVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRI Sbjct: 122 PKCENYEMGFCPLGSSCPRRHIKKVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRI 181 Query: 182 KRDDEINTRKMDEEKERRLNAIINGEV 208 KRDDEINTRKMDEEKERRLNAIINGEV Sbjct: 182 KRDDEINTRKMDEEKERRLNAIINGEV 208 >6DNH-C nrPDB Length = 245 Score = 230 bits (586), Expect = 4e-61, Method: Composition-based stats. Identities = 68/141 (48%), Positives = 91/141 (64%), Gaps = 8/141 (5%) Query: 33 ICEFYNSREGPKSCPRGPLCPKKHVLPIFQNKIVCRHWLRGLCKKNDQCEYLHEYNLRKM 92 +CEF+ +C +G +CP +H+ + +VC+HWLRGLCKK DQCE+LHEY++ KM Sbjct: 41 VCEFFLK----AACGKGGMCPFRHISG--EKTVVCKHWLRGLCKKGDQCEFLHEYDMTKM 94 Query: 93 PECVFFSKNGYCTQSPDCQYLHIDPASKIPKCENYEMGFCPLGSSCPRRHIKKVFCQRYM 152 PEC F+SK G C+ +C +LHIDP SKI C Y+ GFC G C RH ++V C Y+ Sbjct: 95 PECYFYSKFGECSNK-ECPFLHIDPESKIKDCPWYDRGFCKHGPLCRHRHTRRVICVNYL 153 Query: 153 TGFCPLGKDECDMEHPQFIIP 173 GFCP G C HP+F +P Sbjct: 154 VGFCPEGPS-CKFMHPRFELP 173 >6G38-A nrPDB Length = 357 Score = 33.4 bits (75), Expect = 0.071, Method: Composition-based stats. Identities = 11/37 (29%), Positives = 17/37 (45%) Query: 94 ECVFFSKNGYCTQSPDCQYLHIDPASKIPKCENYEMG 130 E ++F G C+Q + H P K+ +CE G Sbjct: 16 ENLYFQSMGECSQKGPVPFSHCLPTEKLQRCEKIGEG 52 >4YH8-A nrPDB Length = 216 Score = 33.0 bits (74), Expect = 0.094, Method: Composition-based stats. Identities = 24/91 (26%), Positives = 35/91 (38%), Gaps = 8/91 (8%) Query: 25 FSLDPDRPICEFYNSREGPKSCPRGPLCPKKHVLPIFQNKIVCRHWLRGLC-----KKND 79 + + D+ C FY +C G C +KHV P F I+C + + KK Sbjct: 9 YGTEQDKVNCSFYYK---IGACRHGERCSRKHVKPNFSQTILCPNMYKNPIHEPNGKKFT 65 Query: 80 QCEYLHEYNLRKMPECVFFSKNGYCTQSPDC 110 Q E +++ FSK G Q C Sbjct: 66 QRELAEQFDAFYEDMFCEFSKYGEVEQLVVC 96 >6ID0-O nrPDB Length = 420 Score = 31.9 bits (71), Expect = 0.19, Method: Composition-based stats. Identities = 9/21 (42%), Positives = 15/21 (71%) Query: 66 VCRHWLRGLCKKNDQCEYLHE 86 +C W++G CK+ ++C Y HE Sbjct: 164 ICSFWVKGECKRGEECPYRHE 184 >6QDV-M nrPDB Length = 289 Score = 31.9 bits (71), Expect = 0.23, Method: Composition-based stats. Identities = 9/21 (42%), Positives = 15/21 (71%) Query: 66 VCRHWLRGLCKKNDQCEYLHE 86 +C W++G CK+ ++C Y HE Sbjct: 147 ICSFWVKGECKRGEECPYRHE 167 >4CS7-A nrPDB Length = 189 Score = 31.9 bits (71), Expect = 0.23, Method: Composition-based stats. Identities = 8/29 (27%), Positives = 15/29 (51%) Query: 59 PIFQNKIVCRHWLRGLCKKNDQCEYLHEY 87 K C++ +RG C + +C++ H Y Sbjct: 1 GPMSRKAPCKYEVRGKCNRGSECKFNHNY 29 >3JB9-Y nrPDB Length = 388 Score = 31.5 bits (70), Expect = 0.29, Method: Composition-based stats. Identities = 14/39 (35%), Positives = 23/39 (58%), Gaps = 6/39 (15%) Query: 67 CRHWLRGLCKKNDQCEYLHEYNLRKMP-ECVFFSKNGYC 104 C ++ RG+C + +CEYLH ++P + FF+ N C Sbjct: 117 CLYFARGMCSEGSKCEYLH-----RLPKDTDFFNANVDC 150 >1D2E-A nrPDB Length = 397 Score = 29.2 bits (64), Expect = 1.3, Method: Composition-based stats. Identities = 12/28 (42%), Positives = 17/28 (60%), Gaps = 1/28 (3%) Query: 64 KIVCRHWLRGLCKKNDQCEYL-HEYNLR 90 +V RG+ KK D+CE+L H N+R Sbjct: 219 TVVTGTLERGILKKGDECEFLGHSKNIR 246 >2D9M-A nrPDB Length = 69 Score = 29.2 bits (64), Expect = 1.3, Method: Composition-based stats. Identities = 10/26 (38%), Positives = 11/26 (42%), Gaps = 1/26 (3%) Query: 147 FCQRYMTGFCPLGKDECDMEHPQFII 172 C RYM G CP G C H + Sbjct: 22 ICDRYMNGTCPEGNS-CKFAHGNAEL 46 >5LJ5-M nrPDB Length = 339 Score = 27.6 bits (60), Expect = 4.1, Method: Composition-based stats. Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 1/26 (3%) Query: 95 CVFFSKNGYCTQSPDCQYLHIDPASK 120 C+FF+K G C P C+YLH P + Sbjct: 73 CLFFAK-GMCCLGPKCEYLHHIPDEE 97 >4C3B-A nrPDB Length = 199 Score = 27.3 bits (59), Expect = 5.1, Method: Composition-based stats. Identities = 8/30 (26%), Positives = 13/30 (43%) Query: 58 LPIFQNKIVCRHWLRGLCKKNDQCEYLHEY 87 L + C+ +RG C +C + H Y Sbjct: 3 LGSMSRRNPCKFEIRGHCLNGKRCHFSHNY 32 >3U1M-A nrPDB Length = 240 Score = 27.3 bits (59), Expect = 5.4, Method: Composition-based stats. Identities = 12/26 (46%), Positives = 16/26 (61%), Gaps = 1/26 (3%) Query: 95 CVFFSKNGYCTQSPDCQYLHIDPASK 120 C+FF+K G C P C+YLH P + Sbjct: 73 CLFFAK-GMCCLGPKCEYLHHIPDEE 97 >3UJ2-A nrPDB Length = 449 Score = 26.9 bits (58), Expect = 6.1, Method: Composition-based stats. Identities = 23/79 (29%), Positives = 37/79 (46%), Gaps = 10/79 (12%) Query: 136 SSCPRRHIK-------KVFCQRYMTGFCPLGKDECDMEHPQFIIPDEGSKLRIKRDDEI- 187 C R+ K C+RY G DE D E Q++ + G K+++ DD Sbjct: 283 PKCKRKFASEELVAHWKSLCERYPIVSIEDGLDEEDWEGWQYMTRELGDKIQLVGDDLFV 342 Query: 188 -NTRKMDEE-KERRLNAII 204 NT ++++ KER N+I+ Sbjct: 343 TNTERLNKGIKERCGNSIL 361 >1ZTD-A nrPDB Length = 133 Score = 26.9 bits (58), Expect = 6.7, Method: Composition-based stats. Identities = 14/61 (22%), Positives = 23/61 (37%), Gaps = 3/61 (4%) Query: 55 KHVLPIFQNKIVCRHWLRGLCKKNDQCEYLHEYNLRKMPECVFFSKNGYCTQSPDCQYLH 114 KH + ++ + WL GL + + E + + PE + FSK L Sbjct: 66 KHAKGDYAEALIAKAWLMGLISEREAVEIIKK---NLYPEVLDFSKKKEAIGRALAPLLV 122 Query: 115 I 115 I Sbjct: 123 I 123 >5WDX-A nrPDB Length = 649 Score = 26.5 bits (57), Expect = 8.2, Method: Composition-based stats. Identities = 19/49 (38%), Positives = 22/49 (44%), Gaps = 10/49 (20%) Query: 31 RPICEFYNSREGPKSCPRGPLCPKKHVLPIFQNKIVCRHWLRGLCKKND 79 RPI S GP CPRG HV+ +F VC RG+ K D Sbjct: 148 RPISTLKGSSGGPVLCPRG------HVVGLF-RAAVCS---RGVAKSID 186 >4N0B-A nrPDB Length = 479 Score = 26.5 bits (57), Expect = 8.6, Method: Composition-based stats. Identities = 10/32 (31%), Positives = 18/32 (56%) Query: 166 EHPQFIIPDEGSKLRIKRDDEINTRKMDEEKE 197 EHP F +PD+ ++ I + D I+ M + + Sbjct: 89 EHPPFALPDDLKEIHIDQSDWISFSHMSSDTD 120 Database: ../databases/nrPDB-GO_2019.06.18_sequences.fasta Posted date: Jan 3, 2023 9:05 PM Number of letters in database: 10,336,785 Number of sequences in database: 36,641 Lambda K H 0.320 0.141 0.465 Lambda K H 0.267 0.0419 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Hits to DB: 10,256,283 Number of Sequences: 36641 Number of extensions: 469670 Number of successful extensions: 979 Number of sequences better than 10.0: 25 Number of HSP's better than 10.0 without gapping: 26 Number of HSP's successfully gapped in prelim test: 17 Number of HSP's that attempted gapping in prelim test: 883 Number of HSP's gapped (non-prelim): 76 length of query: 208 length of database: 10,336,785 effective HSP length: 93 effective length of query: 115 effective length of database: 6,929,172 effective search space: 796854780 effective search space used: 796854780 T: 11 A: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 57 (26.5 bits)